Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase

Autores
Muñoz Labrador, Ana; Doyagüez, Elisa G.; Azcarate, Silvana Mariela; Julio Gonzalez, Cristina; Barile, Daniela; Moreno, F. Javier; Hernandez-Hernandez, Oswaldo
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polyols, or sugar alcohols, are widely used in the industry as sweeteners and food formulation ingredients, aiming to combat the incidence of diet-related Non-Communicable Diseases. Given the attractive use of Generally Regarded As Safe (GRAS) enzymes in both academia and industry, this study reports on an optimized process to achieve polyols transglucosylation using a dextransucrase enzyme derived from Leuconostoc mesenteroides. These enzyme modifications could lead to the creation of a new generation of glucosylated polyols with isomalto-oligosaccharides (IMOS) structures, potentially offering added functionalities such as prebiotic effects. These reactions were guided by a design of experiment framework, aimed at maximizing the yields of potential new sweeteners. Under the optimized conditions, dextransucrase first cleared the glycosidic bond of sucrose, releasing fructose with the formation of an enzyme-glucosyl covalent intermediate complex. Then, the acceptor substrate (i.e., polyols) is bound to the enzyme-glucosyl intermediate, resulting in the transfer of glucosyl unit to the tested polyols. Structural insights into the reaction products were obtained through nuclear maneic resonance (NMR) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analyses, which revealed the presence of linear α(1 → 6) glycosidic linkages attached to the polyols, yielding oligosaccharide structures containing from 4 to 10 glucose residues. These new polyols-based oligosaccharides hold promise as innovative prebiotic sweeteners, potentially offering valuable health benefits.
Fil: Muñoz Labrador, Ana. Consejo Superior de Investigaciones Científicas; España
Fil: Doyagüez, Elisa G.. Consejo Superior de Investigaciones Científicas; España
Fil: Azcarate, Silvana Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Julio Gonzalez, Cristina. Consejo Superior de Investigaciones Científicas; España
Fil: Barile, Daniela. University of California at Davis; Estados Unidos
Fil: Moreno, F. Javier. Consejo Superior de Investigaciones Científicas; España
Fil: Hernandez-Hernandez, Oswaldo. University of California at Davis; Estados Unidos. Consejo Superior de Investigaciones Científicas; España
Materia
SWEETENER
PREBIOTIC
GLUCO-OLIGOSACCHARIDES
ISOMALTO-OLIGOSACCHARIDES
ACCEPTOR REACTION
GLUCOSYLATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/267436
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/267436
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides DextransucraseMuñoz Labrador, AnaDoyagüez, Elisa G.Azcarate, Silvana MarielaJulio Gonzalez, CristinaBarile, DanielaMoreno, F. JavierHernandez-Hernandez, OswaldoSWEETENERPREBIOTICGLUCO-OLIGOSACCHARIDESISOMALTO-OLIGOSACCHARIDESACCEPTOR REACTIONGLUCOSYLATIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Polyols, or sugar alcohols, are widely used in the industry as sweeteners and food formulation ingredients, aiming to combat the incidence of diet-related Non-Communicable Diseases. Given the attractive use of Generally Regarded As Safe (GRAS) enzymes in both academia and industry, this study reports on an optimized process to achieve polyols transglucosylation using a dextransucrase enzyme derived from Leuconostoc mesenteroides. These enzyme modifications could lead to the creation of a new generation of glucosylated polyols with isomalto-oligosaccharides (IMOS) structures, potentially offering added functionalities such as prebiotic effects. These reactions were guided by a design of experiment framework, aimed at maximizing the yields of potential new sweeteners. Under the optimized conditions, dextransucrase first cleared the glycosidic bond of sucrose, releasing fructose with the formation of an enzyme-glucosyl covalent intermediate complex. Then, the acceptor substrate (i.e., polyols) is bound to the enzyme-glucosyl intermediate, resulting in the transfer of glucosyl unit to the tested polyols. Structural insights into the reaction products were obtained through nuclear maneic resonance (NMR) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analyses, which revealed the presence of linear α(1 → 6) glycosidic linkages attached to the polyols, yielding oligosaccharide structures containing from 4 to 10 glucose residues. These new polyols-based oligosaccharides hold promise as innovative prebiotic sweeteners, potentially offering valuable health benefits.Fil: Muñoz Labrador, Ana. Consejo Superior de Investigaciones Científicas; EspañaFil: Doyagüez, Elisa G.. Consejo Superior de Investigaciones Científicas; EspañaFil: Azcarate, Silvana Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Julio Gonzalez, Cristina. Consejo Superior de Investigaciones Científicas; EspañaFil: Barile, Daniela. University of California at Davis; Estados UnidosFil: Moreno, F. Javier. Consejo Superior de Investigaciones Científicas; EspañaFil: Hernandez-Hernandez, Oswaldo. University of California at Davis; Estados Unidos. Consejo Superior de Investigaciones Científicas; EspañaAmerican Chemical Society2024-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267436Muñoz Labrador, Ana; Doyagüez, Elisa G.; Azcarate, Silvana Mariela; Julio Gonzalez, Cristina; Barile, Daniela; et al.; Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase; American Chemical Society; Journal of Agricultural and Food Chemistry; 72; 39; 9-2024; 21690-217010021-8561CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.jafc.4c04222info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jafc.4c04222info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:38Zoai:ri.conicet.gov.ar:11336/267436instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:38.853CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase
title Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase
spellingShingle Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase
Muñoz Labrador, Ana
SWEETENER
PREBIOTIC
GLUCO-OLIGOSACCHARIDES
ISOMALTO-OLIGOSACCHARIDES
ACCEPTOR REACTION
GLUCOSYLATION
title_short Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase
title_full Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase
title_fullStr Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase
title_full_unstemmed Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase
title_sort Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase
dc.creator.none.fl_str_mv Muñoz Labrador, Ana
Doyagüez, Elisa G.
Azcarate, Silvana Mariela
Julio Gonzalez, Cristina
Barile, Daniela
Moreno, F. Javier
Hernandez-Hernandez, Oswaldo
author Muñoz Labrador, Ana
author_facet Muñoz Labrador, Ana
Doyagüez, Elisa G.
Azcarate, Silvana Mariela
Julio Gonzalez, Cristina
Barile, Daniela
Moreno, F. Javier
Hernandez-Hernandez, Oswaldo
author_role author
author2 Doyagüez, Elisa G.
Azcarate, Silvana Mariela
Julio Gonzalez, Cristina
Barile, Daniela
Moreno, F. Javier
Hernandez-Hernandez, Oswaldo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv SWEETENER
PREBIOTIC
GLUCO-OLIGOSACCHARIDES
ISOMALTO-OLIGOSACCHARIDES
ACCEPTOR REACTION
GLUCOSYLATION
topic SWEETENER
PREBIOTIC
GLUCO-OLIGOSACCHARIDES
ISOMALTO-OLIGOSACCHARIDES
ACCEPTOR REACTION
GLUCOSYLATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Polyols, or sugar alcohols, are widely used in the industry as sweeteners and food formulation ingredients, aiming to combat the incidence of diet-related Non-Communicable Diseases. Given the attractive use of Generally Regarded As Safe (GRAS) enzymes in both academia and industry, this study reports on an optimized process to achieve polyols transglucosylation using a dextransucrase enzyme derived from Leuconostoc mesenteroides. These enzyme modifications could lead to the creation of a new generation of glucosylated polyols with isomalto-oligosaccharides (IMOS) structures, potentially offering added functionalities such as prebiotic effects. These reactions were guided by a design of experiment framework, aimed at maximizing the yields of potential new sweeteners. Under the optimized conditions, dextransucrase first cleared the glycosidic bond of sucrose, releasing fructose with the formation of an enzyme-glucosyl covalent intermediate complex. Then, the acceptor substrate (i.e., polyols) is bound to the enzyme-glucosyl intermediate, resulting in the transfer of glucosyl unit to the tested polyols. Structural insights into the reaction products were obtained through nuclear maneic resonance (NMR) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analyses, which revealed the presence of linear α(1 → 6) glycosidic linkages attached to the polyols, yielding oligosaccharide structures containing from 4 to 10 glucose residues. These new polyols-based oligosaccharides hold promise as innovative prebiotic sweeteners, potentially offering valuable health benefits.
Fil: Muñoz Labrador, Ana. Consejo Superior de Investigaciones Científicas; España
Fil: Doyagüez, Elisa G.. Consejo Superior de Investigaciones Científicas; España
Fil: Azcarate, Silvana Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Julio Gonzalez, Cristina. Consejo Superior de Investigaciones Científicas; España
Fil: Barile, Daniela. University of California at Davis; Estados Unidos
Fil: Moreno, F. Javier. Consejo Superior de Investigaciones Científicas; España
Fil: Hernandez-Hernandez, Oswaldo. University of California at Davis; Estados Unidos. Consejo Superior de Investigaciones Científicas; España
description Polyols, or sugar alcohols, are widely used in the industry as sweeteners and food formulation ingredients, aiming to combat the incidence of diet-related Non-Communicable Diseases. Given the attractive use of Generally Regarded As Safe (GRAS) enzymes in both academia and industry, this study reports on an optimized process to achieve polyols transglucosylation using a dextransucrase enzyme derived from Leuconostoc mesenteroides. These enzyme modifications could lead to the creation of a new generation of glucosylated polyols with isomalto-oligosaccharides (IMOS) structures, potentially offering added functionalities such as prebiotic effects. These reactions were guided by a design of experiment framework, aimed at maximizing the yields of potential new sweeteners. Under the optimized conditions, dextransucrase first cleared the glycosidic bond of sucrose, releasing fructose with the formation of an enzyme-glucosyl covalent intermediate complex. Then, the acceptor substrate (i.e., polyols) is bound to the enzyme-glucosyl intermediate, resulting in the transfer of glucosyl unit to the tested polyols. Structural insights into the reaction products were obtained through nuclear maneic resonance (NMR) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analyses, which revealed the presence of linear α(1 → 6) glycosidic linkages attached to the polyols, yielding oligosaccharide structures containing from 4 to 10 glucose residues. These new polyols-based oligosaccharides hold promise as innovative prebiotic sweeteners, potentially offering valuable health benefits.
publishDate 2024
dc.date.none.fl_str_mv 2024-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/267436
Muñoz Labrador, Ana; Doyagüez, Elisa G.; Azcarate, Silvana Mariela; Julio Gonzalez, Cristina; Barile, Daniela; et al.; Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase; American Chemical Society; Journal of Agricultural and Food Chemistry; 72; 39; 9-2024; 21690-21701
0021-8561
CONICET Digital
CONICET
url http://hdl.handle.net/11336/267436
identifier_str_mv Muñoz Labrador, Ana; Doyagüez, Elisa G.; Azcarate, Silvana Mariela; Julio Gonzalez, Cristina; Barile, Daniela; et al.; Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase; American Chemical Society; Journal of Agricultural and Food Chemistry; 72; 39; 9-2024; 21690-21701
0021-8561
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.jafc.4c04222
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jafc.4c04222
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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