Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit platelets

Autores
Tavares, Flávio L.; Peichoto, María Elisa; Rangel, Danieli de Morais; Barbaro, Kátia Cristina; Cirillo, Maria Cristina; Santoro, Marcelo Larami; Sano Martins, Ida S.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Loxosceles venoms can promote severe local and systemic damages. We have previously reported that Loxosceles gaucho spider venom causes a severe early thrombocytopenia in rabbits. Herein, we investigated the in vitro effects of this venom and its sphingomyelinase fraction on the main functions of platelets. Whole venom and its fraction induced aggregation of both human and rabbit platelets. Aggregation was dependent of plasma component(s) but independent of venom-induced lysophosphatidic acid generation. There was no increase in the levels of lactate dehydrogenase during platelet aggregation, ruling out the possibility of platelet lysis. The increased expression of ligand-induced binding site 1 (LIBS1) induced by L. gaucho venom and its sphingomyelinase fraction, as well as of P-selectin by the whole venom, evidenced the activation state of both human and rabbit platelets. Adhesion assays showed an irregular response when platelets were exposed to the whole venom, whereas the sphingomyelinase fraction induced a dose-dependent increase in the platelet adhesion to collagen. These findings evidence that L. gaucho venom and its sphingomyelinase fraction trigger adhesion, activation, and aggregation of both human and rabbit platelets. Thus, this work justifies the use of rabbits to investigate Loxosceles venom-induced platelet disturbances, and it also supports research on the role of platelets in the pathogenesis of loxoscelism.
Fil: Tavares, Flávio L.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Peichoto, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Rangel, Danieli de Morais. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Barbaro, Kátia Cristina. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Cirillo, Maria Cristina. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Santoro, Marcelo Larami. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Sano Martins, Ida S.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Materia
Loxosceles Gaucho
Venom
Sphingomyelinase
Platelet Activation
Platelet Adhesion
Platelet Aggregation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43597
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43597
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit plateletsTavares, Flávio L.Peichoto, María ElisaRangel, Danieli de MoraisBarbaro, Kátia CristinaCirillo, Maria CristinaSantoro, Marcelo LaramiSano Martins, Ida S.Loxosceles GauchoVenomSphingomyelinasePlatelet ActivationPlatelet AdhesionPlatelet Aggregationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Loxosceles venoms can promote severe local and systemic damages. We have previously reported that Loxosceles gaucho spider venom causes a severe early thrombocytopenia in rabbits. Herein, we investigated the in vitro effects of this venom and its sphingomyelinase fraction on the main functions of platelets. Whole venom and its fraction induced aggregation of both human and rabbit platelets. Aggregation was dependent of plasma component(s) but independent of venom-induced lysophosphatidic acid generation. There was no increase in the levels of lactate dehydrogenase during platelet aggregation, ruling out the possibility of platelet lysis. The increased expression of ligand-induced binding site 1 (LIBS1) induced by L. gaucho venom and its sphingomyelinase fraction, as well as of P-selectin by the whole venom, evidenced the activation state of both human and rabbit platelets. Adhesion assays showed an irregular response when platelets were exposed to the whole venom, whereas the sphingomyelinase fraction induced a dose-dependent increase in the platelet adhesion to collagen. These findings evidence that L. gaucho venom and its sphingomyelinase fraction trigger adhesion, activation, and aggregation of both human and rabbit platelets. Thus, this work justifies the use of rabbits to investigate Loxosceles venom-induced platelet disturbances, and it also supports research on the role of platelets in the pathogenesis of loxoscelism.Fil: Tavares, Flávio L.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Peichoto, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Rangel, Danieli de Morais. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Barbaro, Kátia Cristina. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Cirillo, Maria Cristina. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Santoro, Marcelo Larami. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Sano Martins, Ida S.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilSage Publications Ltd2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43597Tavares, Flávio L.; Peichoto, María Elisa; Rangel, Danieli de Morais; Barbaro, Kátia Cristina; Cirillo, Maria Cristina; et al.; Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit platelets; Sage Publications Ltd; Human and Experimental Toxicoloxy; 30; 10; 1-2011; 1567-15740960-3271CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.1177/0960327110393761info:eu-repo/semantics/altIdentifier/doi/10.1177/0960327110393761info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:39Zoai:ri.conicet.gov.ar:11336/43597instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:39.737CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit platelets
title Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit platelets
spellingShingle Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit platelets
Tavares, Flávio L.
Loxosceles Gaucho
Venom
Sphingomyelinase
Platelet Activation
Platelet Adhesion
Platelet Aggregation
title_short Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit platelets
title_full Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit platelets
title_fullStr Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit platelets
title_full_unstemmed Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit platelets
title_sort Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit platelets
dc.creator.none.fl_str_mv Tavares, Flávio L.
Peichoto, María Elisa
Rangel, Danieli de Morais
Barbaro, Kátia Cristina
Cirillo, Maria Cristina
Santoro, Marcelo Larami
Sano Martins, Ida S.
author Tavares, Flávio L.
author_facet Tavares, Flávio L.
Peichoto, María Elisa
Rangel, Danieli de Morais
Barbaro, Kátia Cristina
Cirillo, Maria Cristina
Santoro, Marcelo Larami
Sano Martins, Ida S.
author_role author
author2 Peichoto, María Elisa
Rangel, Danieli de Morais
Barbaro, Kátia Cristina
Cirillo, Maria Cristina
Santoro, Marcelo Larami
Sano Martins, Ida S.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Loxosceles Gaucho
Venom
Sphingomyelinase
Platelet Activation
Platelet Adhesion
Platelet Aggregation
topic Loxosceles Gaucho
Venom
Sphingomyelinase
Platelet Activation
Platelet Adhesion
Platelet Aggregation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Loxosceles venoms can promote severe local and systemic damages. We have previously reported that Loxosceles gaucho spider venom causes a severe early thrombocytopenia in rabbits. Herein, we investigated the in vitro effects of this venom and its sphingomyelinase fraction on the main functions of platelets. Whole venom and its fraction induced aggregation of both human and rabbit platelets. Aggregation was dependent of plasma component(s) but independent of venom-induced lysophosphatidic acid generation. There was no increase in the levels of lactate dehydrogenase during platelet aggregation, ruling out the possibility of platelet lysis. The increased expression of ligand-induced binding site 1 (LIBS1) induced by L. gaucho venom and its sphingomyelinase fraction, as well as of P-selectin by the whole venom, evidenced the activation state of both human and rabbit platelets. Adhesion assays showed an irregular response when platelets were exposed to the whole venom, whereas the sphingomyelinase fraction induced a dose-dependent increase in the platelet adhesion to collagen. These findings evidence that L. gaucho venom and its sphingomyelinase fraction trigger adhesion, activation, and aggregation of both human and rabbit platelets. Thus, this work justifies the use of rabbits to investigate Loxosceles venom-induced platelet disturbances, and it also supports research on the role of platelets in the pathogenesis of loxoscelism.
Fil: Tavares, Flávio L.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Peichoto, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Rangel, Danieli de Morais. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Barbaro, Kátia Cristina. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Cirillo, Maria Cristina. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Santoro, Marcelo Larami. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Sano Martins, Ida S.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
description Loxosceles venoms can promote severe local and systemic damages. We have previously reported that Loxosceles gaucho spider venom causes a severe early thrombocytopenia in rabbits. Herein, we investigated the in vitro effects of this venom and its sphingomyelinase fraction on the main functions of platelets. Whole venom and its fraction induced aggregation of both human and rabbit platelets. Aggregation was dependent of plasma component(s) but independent of venom-induced lysophosphatidic acid generation. There was no increase in the levels of lactate dehydrogenase during platelet aggregation, ruling out the possibility of platelet lysis. The increased expression of ligand-induced binding site 1 (LIBS1) induced by L. gaucho venom and its sphingomyelinase fraction, as well as of P-selectin by the whole venom, evidenced the activation state of both human and rabbit platelets. Adhesion assays showed an irregular response when platelets were exposed to the whole venom, whereas the sphingomyelinase fraction induced a dose-dependent increase in the platelet adhesion to collagen. These findings evidence that L. gaucho venom and its sphingomyelinase fraction trigger adhesion, activation, and aggregation of both human and rabbit platelets. Thus, this work justifies the use of rabbits to investigate Loxosceles venom-induced platelet disturbances, and it also supports research on the role of platelets in the pathogenesis of loxoscelism.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43597
Tavares, Flávio L.; Peichoto, María Elisa; Rangel, Danieli de Morais; Barbaro, Kátia Cristina; Cirillo, Maria Cristina; et al.; Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit platelets; Sage Publications Ltd; Human and Experimental Toxicoloxy; 30; 10; 1-2011; 1567-1574
0960-3271
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43597
identifier_str_mv Tavares, Flávio L.; Peichoto, María Elisa; Rangel, Danieli de Morais; Barbaro, Kátia Cristina; Cirillo, Maria Cristina; et al.; Loxosceles gaucho spider venom and its sphingomyelinase fraction trigger the main functions of human and rabbit platelets; Sage Publications Ltd; Human and Experimental Toxicoloxy; 30; 10; 1-2011; 1567-1574
0960-3271
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.1177/0960327110393761
info:eu-repo/semantics/altIdentifier/doi/10.1177/0960327110393761
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sage Publications Ltd
publisher.none.fl_str_mv Sage Publications Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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