Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom

Autores
Tavares, F. L.; Peichoto, María Elisa; Marcelino, J. R.; Barbaro, K. C.; Cirillo, M. C.; Santoro, Marcelo Larami; Sano Martins, I. S.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Loxosceles gaucho spider venom induces in vitro platelet activation and marked thrombocytopenia in rabbits. Herein, we investigated the involvement of platelets in the development of the dermonecrosis induced by L. gaucho venom, using thrombocytopenic rabbits as a model. L. gaucho venom evoked a drop in platelet and neutrophil counts 4 h after venom injection. Ecchymotic areas at the site of venom inoculation were noticed as soon as 4 h in thrombocytopenic animals but not in animals with initial normal platelet counts. After 5 days, areas of scars in thrombocytopenic animals were also larger, evidencing the marked development of lesions in the condition of thrombocytopenia. Histologically, local hemorrhage, collagen fiber disorganization, and edema were more severe in thrombocytopenic animals. Leukocyte infiltration, predominantly due to polymorphonuclears, was observed in the presence or not of thrombocytopenia. Thrombus formation was demonstrated by immunohistochemistry at the microvasculature, and it occurred even under marked thrombocytopenia. Taken together, platelets have an important role in minimizing not only the hemorrhagic phenomena but also the inflammatory and wound-healing processes, suggesting that cutaneous loxoscelism may be aggravated under thrombocytopenic conditions.
Fil: Tavares, F. L.. Instituto Butantan. Laboratório de Fisiopatologia; Brasil
Fil: Peichoto, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Medicina Tropical ; Argentina
Fil: Marcelino, J. R.. Instituto Butantan. Divisão de Desenvolvimento Tecnológico e Produção; Brasil
Fil: Barbaro, K. C.. Instituto Butantan. Laboratório de Imunopatologia; Brasil
Fil: Cirillo, M. C.. Instituto Butantan. Laboratório de Fisiopatologia; Brasil
Fil: Santoro, Marcelo Larami. Instituto Butantan. Laboratório de Fisiopatologia; Brasil
Fil: Sano Martins, I. S.. Instituto Butantan. Laboratório de Fisiopatologia; Brasil
Materia
Dermonecrosis
Loxosceles
Platelets
Spider Venom
Thrombocytopenia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37554
Acceder
id CONICETDig_472d0b7bc48d4181f98dcfd4abbcb258
oai_identifier_str oai:ri.conicet.gov.ar:11336/37554
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venomTavares, F. L.Peichoto, María ElisaMarcelino, J. R.Barbaro, K. C.Cirillo, M. C.Santoro, Marcelo LaramiSano Martins, I. S.DermonecrosisLoxoscelesPlateletsSpider VenomThrombocytopeniahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Loxosceles gaucho spider venom induces in vitro platelet activation and marked thrombocytopenia in rabbits. Herein, we investigated the involvement of platelets in the development of the dermonecrosis induced by L. gaucho venom, using thrombocytopenic rabbits as a model. L. gaucho venom evoked a drop in platelet and neutrophil counts 4 h after venom injection. Ecchymotic areas at the site of venom inoculation were noticed as soon as 4 h in thrombocytopenic animals but not in animals with initial normal platelet counts. After 5 days, areas of scars in thrombocytopenic animals were also larger, evidencing the marked development of lesions in the condition of thrombocytopenia. Histologically, local hemorrhage, collagen fiber disorganization, and edema were more severe in thrombocytopenic animals. Leukocyte infiltration, predominantly due to polymorphonuclears, was observed in the presence or not of thrombocytopenia. Thrombus formation was demonstrated by immunohistochemistry at the microvasculature, and it occurred even under marked thrombocytopenia. Taken together, platelets have an important role in minimizing not only the hemorrhagic phenomena but also the inflammatory and wound-healing processes, suggesting that cutaneous loxoscelism may be aggravated under thrombocytopenic conditions.Fil: Tavares, F. L.. Instituto Butantan. Laboratório de Fisiopatologia; BrasilFil: Peichoto, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Medicina Tropical ; ArgentinaFil: Marcelino, J. R.. Instituto Butantan. Divisão de Desenvolvimento Tecnológico e Produção; BrasilFil: Barbaro, K. C.. Instituto Butantan. Laboratório de Imunopatologia; BrasilFil: Cirillo, M. C.. Instituto Butantan. Laboratório de Fisiopatologia; BrasilFil: Santoro, Marcelo Larami. Instituto Butantan. Laboratório de Fisiopatologia; BrasilFil: Sano Martins, I. S.. Instituto Butantan. Laboratório de Fisiopatologia; BrasilSage Publications Ltd2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37554Tavares, F. L.; Peichoto, María Elisa; Marcelino, J. R.; Barbaro, K. C.; Cirillo, M. C.; et al.; Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom; Sage Publications Ltd; Human and Experimental Toxicoloxy; 35; 6; 6-2015; 666-6760960-32711477-0903CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1177/0960327115597983info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/0960327115597983info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:28:32Zoai:ri.conicet.gov.ar:11336/37554instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:28:33.066CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom
title Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom
spellingShingle Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom
Tavares, F. L.
Dermonecrosis
Loxosceles
Platelets
Spider Venom
Thrombocytopenia
title_short Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom
title_full Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom
title_fullStr Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom
title_full_unstemmed Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom
title_sort Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom
dc.creator.none.fl_str_mv Tavares, F. L.
Peichoto, María Elisa
Marcelino, J. R.
Barbaro, K. C.
Cirillo, M. C.
Santoro, Marcelo Larami
Sano Martins, I. S.
author Tavares, F. L.
author_facet Tavares, F. L.
Peichoto, María Elisa
Marcelino, J. R.
Barbaro, K. C.
Cirillo, M. C.
Santoro, Marcelo Larami
Sano Martins, I. S.
author_role author
author2 Peichoto, María Elisa
Marcelino, J. R.
Barbaro, K. C.
Cirillo, M. C.
Santoro, Marcelo Larami
Sano Martins, I. S.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Dermonecrosis
Loxosceles
Platelets
Spider Venom
Thrombocytopenia
topic Dermonecrosis
Loxosceles
Platelets
Spider Venom
Thrombocytopenia
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Loxosceles gaucho spider venom induces in vitro platelet activation and marked thrombocytopenia in rabbits. Herein, we investigated the involvement of platelets in the development of the dermonecrosis induced by L. gaucho venom, using thrombocytopenic rabbits as a model. L. gaucho venom evoked a drop in platelet and neutrophil counts 4 h after venom injection. Ecchymotic areas at the site of venom inoculation were noticed as soon as 4 h in thrombocytopenic animals but not in animals with initial normal platelet counts. After 5 days, areas of scars in thrombocytopenic animals were also larger, evidencing the marked development of lesions in the condition of thrombocytopenia. Histologically, local hemorrhage, collagen fiber disorganization, and edema were more severe in thrombocytopenic animals. Leukocyte infiltration, predominantly due to polymorphonuclears, was observed in the presence or not of thrombocytopenia. Thrombus formation was demonstrated by immunohistochemistry at the microvasculature, and it occurred even under marked thrombocytopenia. Taken together, platelets have an important role in minimizing not only the hemorrhagic phenomena but also the inflammatory and wound-healing processes, suggesting that cutaneous loxoscelism may be aggravated under thrombocytopenic conditions.
Fil: Tavares, F. L.. Instituto Butantan. Laboratório de Fisiopatologia; Brasil
Fil: Peichoto, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Medicina Tropical ; Argentina
Fil: Marcelino, J. R.. Instituto Butantan. Divisão de Desenvolvimento Tecnológico e Produção; Brasil
Fil: Barbaro, K. C.. Instituto Butantan. Laboratório de Imunopatologia; Brasil
Fil: Cirillo, M. C.. Instituto Butantan. Laboratório de Fisiopatologia; Brasil
Fil: Santoro, Marcelo Larami. Instituto Butantan. Laboratório de Fisiopatologia; Brasil
Fil: Sano Martins, I. S.. Instituto Butantan. Laboratório de Fisiopatologia; Brasil
description Loxosceles gaucho spider venom induces in vitro platelet activation and marked thrombocytopenia in rabbits. Herein, we investigated the involvement of platelets in the development of the dermonecrosis induced by L. gaucho venom, using thrombocytopenic rabbits as a model. L. gaucho venom evoked a drop in platelet and neutrophil counts 4 h after venom injection. Ecchymotic areas at the site of venom inoculation were noticed as soon as 4 h in thrombocytopenic animals but not in animals with initial normal platelet counts. After 5 days, areas of scars in thrombocytopenic animals were also larger, evidencing the marked development of lesions in the condition of thrombocytopenia. Histologically, local hemorrhage, collagen fiber disorganization, and edema were more severe in thrombocytopenic animals. Leukocyte infiltration, predominantly due to polymorphonuclears, was observed in the presence or not of thrombocytopenia. Thrombus formation was demonstrated by immunohistochemistry at the microvasculature, and it occurred even under marked thrombocytopenia. Taken together, platelets have an important role in minimizing not only the hemorrhagic phenomena but also the inflammatory and wound-healing processes, suggesting that cutaneous loxoscelism may be aggravated under thrombocytopenic conditions.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37554
Tavares, F. L.; Peichoto, María Elisa; Marcelino, J. R.; Barbaro, K. C.; Cirillo, M. C.; et al.; Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom; Sage Publications Ltd; Human and Experimental Toxicoloxy; 35; 6; 6-2015; 666-676
0960-3271
1477-0903
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37554
identifier_str_mv Tavares, F. L.; Peichoto, María Elisa; Marcelino, J. R.; Barbaro, K. C.; Cirillo, M. C.; et al.; Platelet participation in the pathogenesis of dermonecrosis induced by Loxosceles gaucho venom; Sage Publications Ltd; Human and Experimental Toxicoloxy; 35; 6; 6-2015; 666-676
0960-3271
1477-0903
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1177/0960327115597983
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/0960327115597983
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sage Publications Ltd
publisher.none.fl_str_mv Sage Publications Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082749691068416
score 13.22299

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