Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomaces

Autores
Fontana, Ariel Ramón; Antoniolli, Andrea Noelia; Bottini, Ambrosio Rubén
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A rapid and economically affordable reverse-phase chromatographic approach based on a core-shell column with high-performance liquid chromatography multi-wavelength detector (HPLC-MWD) is proposed for the quantification and quality control of multiclass polyphenols (PPs). The separation of 20 relevant polyphenols from grape pomace extracts (GPEs) was achieved in less than 12 min by using a Kinetex C18 column (3.0 mm x 100 mm, 2.6 µm) with a gradient system of ultrapure water (0.1 % formic acid) and acetonitrile, a temperature of 35 °C and a flow rate of 0.8 mL min-1. The maximum backpressure reached was 327 bar, meaning the developed method is adequate for standard HPLC instruments. The applicability of the method was demonstrated by the determination of PPs in GPEs of different red grape varieties. Cabernet Sauvignon GPE showed the highest content of studied PPs (9804.2 µg g-1 GPE) followed by Bonarda GPE (7302.0 µg g-1 GPE). Besides the methodological development for a high throughput routine quality control of GPEs, this is the first report of PPs content for Bonarda and Aspirant Bouchet GPE, so the results add knowledge for these grape varieties cultivated in Argentina.
Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Antoniolli, Andrea Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
Superficially Porous Particles
Grape Pomace Extracts
Characterization
Bonarda
Aspirant Bouchet
Cabernet Sauvignon
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22406
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22406
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomacesFontana, Ariel RamónAntoniolli, Andrea NoeliaBottini, Ambrosio RubénSuperficially Porous ParticlesGrape Pomace ExtractsCharacterizationBonardaAspirant BouchetCabernet Sauvignonhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A rapid and economically affordable reverse-phase chromatographic approach based on a core-shell column with high-performance liquid chromatography multi-wavelength detector (HPLC-MWD) is proposed for the quantification and quality control of multiclass polyphenols (PPs). The separation of 20 relevant polyphenols from grape pomace extracts (GPEs) was achieved in less than 12 min by using a Kinetex C18 column (3.0 mm x 100 mm, 2.6 µm) with a gradient system of ultrapure water (0.1 % formic acid) and acetonitrile, a temperature of 35 °C and a flow rate of 0.8 mL min-1. The maximum backpressure reached was 327 bar, meaning the developed method is adequate for standard HPLC instruments. The applicability of the method was demonstrated by the determination of PPs in GPEs of different red grape varieties. Cabernet Sauvignon GPE showed the highest content of studied PPs (9804.2 µg g-1 GPE) followed by Bonarda GPE (7302.0 µg g-1 GPE). Besides the methodological development for a high throughput routine quality control of GPEs, this is the first report of PPs content for Bonarda and Aspirant Bouchet GPE, so the results add knowledge for these grape varieties cultivated in Argentina.Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Antoniolli, Andrea Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaElsevier2015-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22406Fontana, Ariel Ramón; Antoniolli, Andrea Noelia; Bottini, Ambrosio Rubén; Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomaces; Elsevier; Food Chemistry; 192; 30-6-2015; 1-80308-8146CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2015.06.101info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0308814615009930info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:02Zoai:ri.conicet.gov.ar:11336/22406instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:02.892CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomaces
title Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomaces
spellingShingle Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomaces
Fontana, Ariel Ramón
Superficially Porous Particles
Grape Pomace Extracts
Characterization
Bonarda
Aspirant Bouchet
Cabernet Sauvignon
title_short Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomaces
title_full Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomaces
title_fullStr Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomaces
title_full_unstemmed Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomaces
title_sort Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomaces
dc.creator.none.fl_str_mv Fontana, Ariel Ramón
Antoniolli, Andrea Noelia
Bottini, Ambrosio Rubén
author Fontana, Ariel Ramón
author_facet Fontana, Ariel Ramón
Antoniolli, Andrea Noelia
Bottini, Ambrosio Rubén
author_role author
author2 Antoniolli, Andrea Noelia
Bottini, Ambrosio Rubén
author2_role author
author
dc.subject.none.fl_str_mv Superficially Porous Particles
Grape Pomace Extracts
Characterization
Bonarda
Aspirant Bouchet
Cabernet Sauvignon
topic Superficially Porous Particles
Grape Pomace Extracts
Characterization
Bonarda
Aspirant Bouchet
Cabernet Sauvignon
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A rapid and economically affordable reverse-phase chromatographic approach based on a core-shell column with high-performance liquid chromatography multi-wavelength detector (HPLC-MWD) is proposed for the quantification and quality control of multiclass polyphenols (PPs). The separation of 20 relevant polyphenols from grape pomace extracts (GPEs) was achieved in less than 12 min by using a Kinetex C18 column (3.0 mm x 100 mm, 2.6 µm) with a gradient system of ultrapure water (0.1 % formic acid) and acetonitrile, a temperature of 35 °C and a flow rate of 0.8 mL min-1. The maximum backpressure reached was 327 bar, meaning the developed method is adequate for standard HPLC instruments. The applicability of the method was demonstrated by the determination of PPs in GPEs of different red grape varieties. Cabernet Sauvignon GPE showed the highest content of studied PPs (9804.2 µg g-1 GPE) followed by Bonarda GPE (7302.0 µg g-1 GPE). Besides the methodological development for a high throughput routine quality control of GPEs, this is the first report of PPs content for Bonarda and Aspirant Bouchet GPE, so the results add knowledge for these grape varieties cultivated in Argentina.
Fil: Fontana, Ariel Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Antoniolli, Andrea Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description A rapid and economically affordable reverse-phase chromatographic approach based on a core-shell column with high-performance liquid chromatography multi-wavelength detector (HPLC-MWD) is proposed for the quantification and quality control of multiclass polyphenols (PPs). The separation of 20 relevant polyphenols from grape pomace extracts (GPEs) was achieved in less than 12 min by using a Kinetex C18 column (3.0 mm x 100 mm, 2.6 µm) with a gradient system of ultrapure water (0.1 % formic acid) and acetonitrile, a temperature of 35 °C and a flow rate of 0.8 mL min-1. The maximum backpressure reached was 327 bar, meaning the developed method is adequate for standard HPLC instruments. The applicability of the method was demonstrated by the determination of PPs in GPEs of different red grape varieties. Cabernet Sauvignon GPE showed the highest content of studied PPs (9804.2 µg g-1 GPE) followed by Bonarda GPE (7302.0 µg g-1 GPE). Besides the methodological development for a high throughput routine quality control of GPEs, this is the first report of PPs content for Bonarda and Aspirant Bouchet GPE, so the results add knowledge for these grape varieties cultivated in Argentina.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22406
Fontana, Ariel Ramón; Antoniolli, Andrea Noelia; Bottini, Ambrosio Rubén; Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomaces; Elsevier; Food Chemistry; 192; 30-6-2015; 1-8
0308-8146
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22406
identifier_str_mv Fontana, Ariel Ramón; Antoniolli, Andrea Noelia; Bottini, Ambrosio Rubén; Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid determination of multiclass polyphenols in grape pomaces; Elsevier; Food Chemistry; 192; 30-6-2015; 1-8
0308-8146
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2015.06.101
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0308814615009930
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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