A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanism

Autores
Mustafi, S.; Veisaga, M. L.; Lopez, Luis Alberto; Barbieri, M. A.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Increasing resistance of Pseudomonas aeruginosa (P. aeruginosa) to conventional treatments demands the search for noveltherapeutic strategies. In this study, the antimicrobial activity of dehydroleucodine (DhL), a sesquiterpene lactone obtainedfrom Artemisia (A.) douglasiana, was screened against several pathogenic virulence effectors of P. aeruginosa. In vitro, minimuminhibitory concentration of DhL was determined against P. aeruginosa strains PAO1, PA103, PA14, and multidrug resistant clinicalstrain, CDN118. Results showed that DhL was active against each strain where PAO1 and PA103 showed higher susceptibility (MIC0.48 mg/mL) as compared to PA14 (MIC 0.96 mg/mL) and CDN118 (MIC 0.98 mg/mL). Also, when PAO1 strain was grown inthe presence of DhL (MIC50, 0.12 mg/mL), a delay in the generation time was noticed along with significant inhibition of secretoryprotease and elastase activities, interruption in biofilm attachment phase in a stationary culture, and a significant decline in Type IIIeffector ExoS. At MIC50, DhL treatment increased the sensitivity of P. aeruginosa towards potent antibiotics. Furthermore, treatmentof P. aeruginosa with DhL prevented toxin-induced apoptosis in macrophages. These observations suggest that DhL activity wasat the bacterial transcriptional level. Hence, antimicrobial activity of DhL may serve as leads in the development of new antiPseudomonaspharmaceuticals.
Fil: Mustafi, S.. Florida International University; Estados Unidos
Fil: Veisaga, M. L.. Florida International University; Estados Unidos
Fil: Lopez, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Barbieri, M. A.. Florida International University; Estados Unidos. Fairchild Tropical Botanic Garden; Estados Unidos. Universidad Nacional de Cuyo; Argentina
Materia
DEHYDROLEUCODINE
PSEUDOMONAS AERUGINOSA
ARTEMISIA DOUGLASIANA
VIRULENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40985
Acceder
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network_name_str CONICET Digital (CONICET)
spelling A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanismMustafi, S.Veisaga, M. L.Lopez, Luis AlbertoBarbieri, M. A.DEHYDROLEUCODINEPSEUDOMONAS AERUGINOSAARTEMISIA DOUGLASIANAVIRULENCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Increasing resistance of Pseudomonas aeruginosa (P. aeruginosa) to conventional treatments demands the search for noveltherapeutic strategies. In this study, the antimicrobial activity of dehydroleucodine (DhL), a sesquiterpene lactone obtainedfrom Artemisia (A.) douglasiana, was screened against several pathogenic virulence effectors of P. aeruginosa. In vitro, minimuminhibitory concentration of DhL was determined against P. aeruginosa strains PAO1, PA103, PA14, and multidrug resistant clinicalstrain, CDN118. Results showed that DhL was active against each strain where PAO1 and PA103 showed higher susceptibility (MIC0.48 mg/mL) as compared to PA14 (MIC 0.96 mg/mL) and CDN118 (MIC 0.98 mg/mL). Also, when PAO1 strain was grown inthe presence of DhL (MIC50, 0.12 mg/mL), a delay in the generation time was noticed along with significant inhibition of secretoryprotease and elastase activities, interruption in biofilm attachment phase in a stationary culture, and a significant decline in Type IIIeffector ExoS. At MIC50, DhL treatment increased the sensitivity of P. aeruginosa towards potent antibiotics. Furthermore, treatmentof P. aeruginosa with DhL prevented toxin-induced apoptosis in macrophages. These observations suggest that DhL activity wasat the bacterial transcriptional level. Hence, antimicrobial activity of DhL may serve as leads in the development of new antiPseudomonaspharmaceuticals.Fil: Mustafi, S.. Florida International University; Estados UnidosFil: Veisaga, M. L.. Florida International University; Estados UnidosFil: Lopez, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Barbieri, M. A.. Florida International University; Estados Unidos. Fairchild Tropical Botanic Garden; Estados Unidos. Universidad Nacional de Cuyo; ArgentinaIndawi2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40985Mustafi, S.; Veisaga, M. L.; Lopez, Luis Alberto; Barbieri, M. A.; A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanism; Indawi; BioMed Research International; 2015; 12-2015; 1-112314-61332314-6141CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2015/216097info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2015/216097/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:00:11Zoai:ri.conicet.gov.ar:11336/40985instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:00:12.25CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanism
title A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanism
spellingShingle A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanism
Mustafi, S.
DEHYDROLEUCODINE
PSEUDOMONAS AERUGINOSA
ARTEMISIA DOUGLASIANA
VIRULENCE
title_short A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanism
title_full A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanism
title_fullStr A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanism
title_full_unstemmed A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanism
title_sort A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanism
dc.creator.none.fl_str_mv Mustafi, S.
Veisaga, M. L.
Lopez, Luis Alberto
Barbieri, M. A.
author Mustafi, S.
author_facet Mustafi, S.
Veisaga, M. L.
Lopez, Luis Alberto
Barbieri, M. A.
author_role author
author2 Veisaga, M. L.
Lopez, Luis Alberto
Barbieri, M. A.
author2_role author
author
author
dc.subject.none.fl_str_mv DEHYDROLEUCODINE
PSEUDOMONAS AERUGINOSA
ARTEMISIA DOUGLASIANA
VIRULENCE
topic DEHYDROLEUCODINE
PSEUDOMONAS AERUGINOSA
ARTEMISIA DOUGLASIANA
VIRULENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Increasing resistance of Pseudomonas aeruginosa (P. aeruginosa) to conventional treatments demands the search for noveltherapeutic strategies. In this study, the antimicrobial activity of dehydroleucodine (DhL), a sesquiterpene lactone obtainedfrom Artemisia (A.) douglasiana, was screened against several pathogenic virulence effectors of P. aeruginosa. In vitro, minimuminhibitory concentration of DhL was determined against P. aeruginosa strains PAO1, PA103, PA14, and multidrug resistant clinicalstrain, CDN118. Results showed that DhL was active against each strain where PAO1 and PA103 showed higher susceptibility (MIC0.48 mg/mL) as compared to PA14 (MIC 0.96 mg/mL) and CDN118 (MIC 0.98 mg/mL). Also, when PAO1 strain was grown inthe presence of DhL (MIC50, 0.12 mg/mL), a delay in the generation time was noticed along with significant inhibition of secretoryprotease and elastase activities, interruption in biofilm attachment phase in a stationary culture, and a significant decline in Type IIIeffector ExoS. At MIC50, DhL treatment increased the sensitivity of P. aeruginosa towards potent antibiotics. Furthermore, treatmentof P. aeruginosa with DhL prevented toxin-induced apoptosis in macrophages. These observations suggest that DhL activity wasat the bacterial transcriptional level. Hence, antimicrobial activity of DhL may serve as leads in the development of new antiPseudomonaspharmaceuticals.
Fil: Mustafi, S.. Florida International University; Estados Unidos
Fil: Veisaga, M. L.. Florida International University; Estados Unidos
Fil: Lopez, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Barbieri, M. A.. Florida International University; Estados Unidos. Fairchild Tropical Botanic Garden; Estados Unidos. Universidad Nacional de Cuyo; Argentina
description Increasing resistance of Pseudomonas aeruginosa (P. aeruginosa) to conventional treatments demands the search for noveltherapeutic strategies. In this study, the antimicrobial activity of dehydroleucodine (DhL), a sesquiterpene lactone obtainedfrom Artemisia (A.) douglasiana, was screened against several pathogenic virulence effectors of P. aeruginosa. In vitro, minimuminhibitory concentration of DhL was determined against P. aeruginosa strains PAO1, PA103, PA14, and multidrug resistant clinicalstrain, CDN118. Results showed that DhL was active against each strain where PAO1 and PA103 showed higher susceptibility (MIC0.48 mg/mL) as compared to PA14 (MIC 0.96 mg/mL) and CDN118 (MIC 0.98 mg/mL). Also, when PAO1 strain was grown inthe presence of DhL (MIC50, 0.12 mg/mL), a delay in the generation time was noticed along with significant inhibition of secretoryprotease and elastase activities, interruption in biofilm attachment phase in a stationary culture, and a significant decline in Type IIIeffector ExoS. At MIC50, DhL treatment increased the sensitivity of P. aeruginosa towards potent antibiotics. Furthermore, treatmentof P. aeruginosa with DhL prevented toxin-induced apoptosis in macrophages. These observations suggest that DhL activity wasat the bacterial transcriptional level. Hence, antimicrobial activity of DhL may serve as leads in the development of new antiPseudomonaspharmaceuticals.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40985
Mustafi, S.; Veisaga, M. L.; Lopez, Luis Alberto; Barbieri, M. A.; A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanism; Indawi; BioMed Research International; 2015; 12-2015; 1-11
2314-6133
2314-6141
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40985
identifier_str_mv Mustafi, S.; Veisaga, M. L.; Lopez, Luis Alberto; Barbieri, M. A.; A novel insight into Dehydroleucodine mediated attenuation of Pseudomonas aeruginosa virulence mechanism; Indawi; BioMed Research International; 2015; 12-2015; 1-11
2314-6133
2314-6141
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2015/216097
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2015/216097/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Indawi
publisher.none.fl_str_mv Indawi
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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