Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributes

Autores
Rajkowska, Katarzyna; Otlewska, Anna; Guiamet, Patricia Sandra; Wrzosek, Henryk; Machnowski, Waldemar
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pseudomonas aeruginosa is an opportunistic pathogen associated with a broad spectrum of infections in humans. However, the pathogenicity of environmental P. aeruginosa strains, especially isolates from museums and conservation laboratories, is not widely recognized. In this study, the virulence attributes of P. aeruginosa isolated from pre-Columbian textiles were compared to those of a clinical strain. Both genetically identified environmental strains (KP842564 and KP842565) exhibited a high ability to form biofilms on abiotic surfaces and high hemolytic activity. In addition, strain KP842564 was a moderate pyocyanin producer and showed proteolytic properties toward bovine serum albumin, fibrinogen, mucin, and casein. In contrast to the clinical isolate, the environmental strainswere susceptible to all the tested antimicrobial agents. The strains also showed high bioadhesion and colonization capacity on archeological textile samples, in which wool fibers were the only source of nutrients, as confirmed by scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX) analysis. This study highlights the need to identify microorganisms which inhabit historic objects, in order to avoid exposure to occupational hazards. Although the strain KP842565 exhibited only some of the examined virulence-related features, given that the production of pyocyanin and hemolysins as well as the formation of biofilm are important virulence factors of P. aeruginosa, the results indicate that these strains may present a potential health risk for humans.
Fil: Rajkowska, Katarzyna. Lodz University of Technology; Polonia
Fil: Otlewska, Anna. Lodz University of Technology; Polonia
Fil: Guiamet, Patricia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Wrzosek, Henryk. Lodz University of Technology; Polonia
Fil: Machnowski, Waldemar. Lodz University of Technology; Polonia
Materia
ARCHEOLOGICAL TEXTILE
FTIR
OCCUPATIONAL HAZARD
PSEUDOMONAS AERUGINOSA
SEM-EDX
VIRULENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/141698
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/141698
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributesRajkowska, KatarzynaOtlewska, AnnaGuiamet, Patricia SandraWrzosek, HenrykMachnowski, WaldemarARCHEOLOGICAL TEXTILEFTIROCCUPATIONAL HAZARDPSEUDOMONAS AERUGINOSASEM-EDXVIRULENCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/6.1https://purl.org/becyt/ford/6Pseudomonas aeruginosa is an opportunistic pathogen associated with a broad spectrum of infections in humans. However, the pathogenicity of environmental P. aeruginosa strains, especially isolates from museums and conservation laboratories, is not widely recognized. In this study, the virulence attributes of P. aeruginosa isolated from pre-Columbian textiles were compared to those of a clinical strain. Both genetically identified environmental strains (KP842564 and KP842565) exhibited a high ability to form biofilms on abiotic surfaces and high hemolytic activity. In addition, strain KP842564 was a moderate pyocyanin producer and showed proteolytic properties toward bovine serum albumin, fibrinogen, mucin, and casein. In contrast to the clinical isolate, the environmental strainswere susceptible to all the tested antimicrobial agents. The strains also showed high bioadhesion and colonization capacity on archeological textile samples, in which wool fibers were the only source of nutrients, as confirmed by scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX) analysis. This study highlights the need to identify microorganisms which inhabit historic objects, in order to avoid exposure to occupational hazards. Although the strain KP842565 exhibited only some of the examined virulence-related features, given that the production of pyocyanin and hemolysins as well as the formation of biofilm are important virulence factors of P. aeruginosa, the results indicate that these strains may present a potential health risk for humans.Fil: Rajkowska, Katarzyna. Lodz University of Technology; PoloniaFil: Otlewska, Anna. Lodz University of Technology; PoloniaFil: Guiamet, Patricia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Wrzosek, Henryk. Lodz University of Technology; PoloniaFil: Machnowski, Waldemar. Lodz University of Technology; PoloniaMDPI Multidisciplinary Digital Publishing Institute2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141698Rajkowska, Katarzyna; Otlewska, Anna; Guiamet, Patricia Sandra; Wrzosek, Henryk; Machnowski, Waldemar; Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributes; MDPI Multidisciplinary Digital Publishing Institute; Applied Sciences (Switzerland); 10; 1; 1-2020; 1-112076-3417CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-3417/10/1/116info:eu-repo/semantics/altIdentifier/doi/10.3390/app10010116info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:04:54Zoai:ri.conicet.gov.ar:11336/141698instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:04:55.077CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributes
title Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributes
spellingShingle Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributes
Rajkowska, Katarzyna
ARCHEOLOGICAL TEXTILE
FTIR
OCCUPATIONAL HAZARD
PSEUDOMONAS AERUGINOSA
SEM-EDX
VIRULENCE
title_short Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributes
title_full Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributes
title_fullStr Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributes
title_full_unstemmed Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributes
title_sort Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributes
dc.creator.none.fl_str_mv Rajkowska, Katarzyna
Otlewska, Anna
Guiamet, Patricia Sandra
Wrzosek, Henryk
Machnowski, Waldemar
author Rajkowska, Katarzyna
author_facet Rajkowska, Katarzyna
Otlewska, Anna
Guiamet, Patricia Sandra
Wrzosek, Henryk
Machnowski, Waldemar
author_role author
author2 Otlewska, Anna
Guiamet, Patricia Sandra
Wrzosek, Henryk
Machnowski, Waldemar
author2_role author
author
author
author
dc.subject.none.fl_str_mv ARCHEOLOGICAL TEXTILE
FTIR
OCCUPATIONAL HAZARD
PSEUDOMONAS AERUGINOSA
SEM-EDX
VIRULENCE
topic ARCHEOLOGICAL TEXTILE
FTIR
OCCUPATIONAL HAZARD
PSEUDOMONAS AERUGINOSA
SEM-EDX
VIRULENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/6.1
https://purl.org/becyt/ford/6
dc.description.none.fl_txt_mv Pseudomonas aeruginosa is an opportunistic pathogen associated with a broad spectrum of infections in humans. However, the pathogenicity of environmental P. aeruginosa strains, especially isolates from museums and conservation laboratories, is not widely recognized. In this study, the virulence attributes of P. aeruginosa isolated from pre-Columbian textiles were compared to those of a clinical strain. Both genetically identified environmental strains (KP842564 and KP842565) exhibited a high ability to form biofilms on abiotic surfaces and high hemolytic activity. In addition, strain KP842564 was a moderate pyocyanin producer and showed proteolytic properties toward bovine serum albumin, fibrinogen, mucin, and casein. In contrast to the clinical isolate, the environmental strainswere susceptible to all the tested antimicrobial agents. The strains also showed high bioadhesion and colonization capacity on archeological textile samples, in which wool fibers were the only source of nutrients, as confirmed by scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX) analysis. This study highlights the need to identify microorganisms which inhabit historic objects, in order to avoid exposure to occupational hazards. Although the strain KP842565 exhibited only some of the examined virulence-related features, given that the production of pyocyanin and hemolysins as well as the formation of biofilm are important virulence factors of P. aeruginosa, the results indicate that these strains may present a potential health risk for humans.
Fil: Rajkowska, Katarzyna. Lodz University of Technology; Polonia
Fil: Otlewska, Anna. Lodz University of Technology; Polonia
Fil: Guiamet, Patricia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Wrzosek, Henryk. Lodz University of Technology; Polonia
Fil: Machnowski, Waldemar. Lodz University of Technology; Polonia
description Pseudomonas aeruginosa is an opportunistic pathogen associated with a broad spectrum of infections in humans. However, the pathogenicity of environmental P. aeruginosa strains, especially isolates from museums and conservation laboratories, is not widely recognized. In this study, the virulence attributes of P. aeruginosa isolated from pre-Columbian textiles were compared to those of a clinical strain. Both genetically identified environmental strains (KP842564 and KP842565) exhibited a high ability to form biofilms on abiotic surfaces and high hemolytic activity. In addition, strain KP842564 was a moderate pyocyanin producer and showed proteolytic properties toward bovine serum albumin, fibrinogen, mucin, and casein. In contrast to the clinical isolate, the environmental strainswere susceptible to all the tested antimicrobial agents. The strains also showed high bioadhesion and colonization capacity on archeological textile samples, in which wool fibers were the only source of nutrients, as confirmed by scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX) analysis. This study highlights the need to identify microorganisms which inhabit historic objects, in order to avoid exposure to occupational hazards. Although the strain KP842565 exhibited only some of the examined virulence-related features, given that the production of pyocyanin and hemolysins as well as the formation of biofilm are important virulence factors of P. aeruginosa, the results indicate that these strains may present a potential health risk for humans.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/141698
Rajkowska, Katarzyna; Otlewska, Anna; Guiamet, Patricia Sandra; Wrzosek, Henryk; Machnowski, Waldemar; Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributes; MDPI Multidisciplinary Digital Publishing Institute; Applied Sciences (Switzerland); 10; 1; 1-2020; 1-11
2076-3417
CONICET Digital
CONICET
url http://hdl.handle.net/11336/141698
identifier_str_mv Rajkowska, Katarzyna; Otlewska, Anna; Guiamet, Patricia Sandra; Wrzosek, Henryk; Machnowski, Waldemar; Pre-columbian archeological textiles: A source of pseudomonas aeruginosa with virulence attributes; MDPI Multidisciplinary Digital Publishing Institute; Applied Sciences (Switzerland); 10; 1; 1-2020; 1-11
2076-3417
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-3417/10/1/116
info:eu-repo/semantics/altIdentifier/doi/10.3390/app10010116
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv MDPI Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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