ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium
- Autores
- Kulik, Tomasz; Abarenkov, Kessy; Busko, Maciej; Bilska, Katarzyna; van Diepeningen, Anne D.; Ostrowska-Kolodziejczak, Anna; Krawczyk, Katarzyna; Brankovics, Balázs; Stenglein, Sebastian Alberto; Sawicki, Jakub; Perkowski, Juliusz
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Type B trichothecenes, which pose a serious hazard to consumer health, occur worldwide in grains. These mycotoxins are produced mainly by three different trichothecene genotypes/chemotypes: 3ADON (3-acetyldeoxynivalenol), 15ADON (15-acetyldeoxynivalenol) and NIV (nivalenol), named after these three major mycotoxin compounds. Correct identification of these genotypes is elementary for all studies relating to population surveys, fungal ecology and mycotoxicology. Trichothecene producers exhibit enormous strain-dependent chemical diversity, which may result in variation in levels of the genotype´s determining toxin and in the production of low to high amounts of atypical compounds. New high-throughput DNA-sequencing technologies promise to boost the diagnostics of mycotoxin genotypes. However, this requires a reference database containing a satisfactory taxonomic sampling of sequences showing high correlation to actually produced chemotypes. We believe that one of the most pressing current challenges of such a database is the linking of molecular identification with chemical diversity of the strains, as well as other metadata. In this study, we use the Tri12 gene involved in mycotoxin biosynthesis for identification of Tri genotypes through sequence comparison. Tri12 sequences from a range of geographically diverse fungal strains comprising 22 Fusarium species were stored in the ToxGen database, which covers descriptive and up-to-date annotations such as indication on Tri genotype and chemotype of the strains, chemical diversity, information on trichothecene-inducing host, substrate or media, geographical locality, and most recent taxonomic affiliations. The present initiative bridges the gap between the demands of comprehensive studies on trichothecene producers and the existing nucleotide sequence databases, which lack toxicological and other auxiliary data. We invite researchers working in the fields of fungal taxonomy, epidemiology and mycotoxicology to join the freely available annotation effort.
Fil: Kulik, Tomasz. Uniwersytet Warminsko-mazurski W Olsztynie;
Fil: Abarenkov, Kessy. University Of Tartu.; Estonia
Fil: Busko, Maciej. Poznań University of Life Sciences; Polonia
Fil: Bilska, Katarzyna. University of Warmia and Mazury; Polonia
Fil: van Diepeningen, Anne D.. University of Amsterdam; Países Bajos
Fil: Ostrowska-Kolodziejczak, Anna. Poznań University of Life Science; Polonia
Fil: Krawczyk, Katarzyna. University of Warmia and Mazur; Polonia
Fil: Brankovics, Balázs. CBS-KNAW Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países Bajos
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Sawicki, Jakub. University of Warmia and Mazury; Polonia
Fil: Perkowski, Juliusz. Poznań University of Life Sciences; Polonia - Materia
-
ANNOTATION
CHEMOTYPES
FUSARIUM
MOLECULAR IDENTIFICATION
TRICHOTHECENE GENOTYPES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/30587
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in FusariumKulik, TomaszAbarenkov, KessyBusko, MaciejBilska, Katarzynavan Diepeningen, Anne D.Ostrowska-Kolodziejczak, AnnaKrawczyk, KatarzynaBrankovics, BalázsStenglein, Sebastian AlbertoSawicki, JakubPerkowski, JuliuszANNOTATIONCHEMOTYPESFUSARIUMMOLECULAR IDENTIFICATIONTRICHOTHECENE GENOTYPEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.2https://purl.org/becyt/ford/1Type B trichothecenes, which pose a serious hazard to consumer health, occur worldwide in grains. These mycotoxins are produced mainly by three different trichothecene genotypes/chemotypes: 3ADON (3-acetyldeoxynivalenol), 15ADON (15-acetyldeoxynivalenol) and NIV (nivalenol), named after these three major mycotoxin compounds. Correct identification of these genotypes is elementary for all studies relating to population surveys, fungal ecology and mycotoxicology. Trichothecene producers exhibit enormous strain-dependent chemical diversity, which may result in variation in levels of the genotype´s determining toxin and in the production of low to high amounts of atypical compounds. New high-throughput DNA-sequencing technologies promise to boost the diagnostics of mycotoxin genotypes. However, this requires a reference database containing a satisfactory taxonomic sampling of sequences showing high correlation to actually produced chemotypes. We believe that one of the most pressing current challenges of such a database is the linking of molecular identification with chemical diversity of the strains, as well as other metadata. In this study, we use the Tri12 gene involved in mycotoxin biosynthesis for identification of Tri genotypes through sequence comparison. Tri12 sequences from a range of geographically diverse fungal strains comprising 22 Fusarium species were stored in the ToxGen database, which covers descriptive and up-to-date annotations such as indication on Tri genotype and chemotype of the strains, chemical diversity, information on trichothecene-inducing host, substrate or media, geographical locality, and most recent taxonomic affiliations. The present initiative bridges the gap between the demands of comprehensive studies on trichothecene producers and the existing nucleotide sequence databases, which lack toxicological and other auxiliary data. We invite researchers working in the fields of fungal taxonomy, epidemiology and mycotoxicology to join the freely available annotation effort.Fil: Kulik, Tomasz. Uniwersytet Warminsko-mazurski W Olsztynie;Fil: Abarenkov, Kessy. University Of Tartu.; EstoniaFil: Busko, Maciej. Poznań University of Life Sciences; PoloniaFil: Bilska, Katarzyna. University of Warmia and Mazury; PoloniaFil: van Diepeningen, Anne D.. University of Amsterdam; Países BajosFil: Ostrowska-Kolodziejczak, Anna. Poznań University of Life Science; PoloniaFil: Krawczyk, Katarzyna. University of Warmia and Mazur; PoloniaFil: Brankovics, Balázs. CBS-KNAW Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países BajosFil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaFil: Sawicki, Jakub. University of Warmia and Mazury; PoloniaFil: Perkowski, Juliusz. Poznań University of Life Sciences; PoloniaPeerJ Inc2017-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30587Kulik, Tomasz; Abarenkov, Kessy; Busko, Maciej; Bilska, Katarzyna; van Diepeningen, Anne D.; et al.; ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium; PeerJ Inc; Peer J; 2017; 2; 2-2017; 1-192167-8359CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.2992info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:33Zoai:ri.conicet.gov.ar:11336/30587instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:34.051CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium |
title |
ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium |
spellingShingle |
ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium Kulik, Tomasz ANNOTATION CHEMOTYPES FUSARIUM MOLECULAR IDENTIFICATION TRICHOTHECENE GENOTYPES |
title_short |
ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium |
title_full |
ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium |
title_fullStr |
ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium |
title_full_unstemmed |
ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium |
title_sort |
ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium |
dc.creator.none.fl_str_mv |
Kulik, Tomasz Abarenkov, Kessy Busko, Maciej Bilska, Katarzyna van Diepeningen, Anne D. Ostrowska-Kolodziejczak, Anna Krawczyk, Katarzyna Brankovics, Balázs Stenglein, Sebastian Alberto Sawicki, Jakub Perkowski, Juliusz |
author |
Kulik, Tomasz |
author_facet |
Kulik, Tomasz Abarenkov, Kessy Busko, Maciej Bilska, Katarzyna van Diepeningen, Anne D. Ostrowska-Kolodziejczak, Anna Krawczyk, Katarzyna Brankovics, Balázs Stenglein, Sebastian Alberto Sawicki, Jakub Perkowski, Juliusz |
author_role |
author |
author2 |
Abarenkov, Kessy Busko, Maciej Bilska, Katarzyna van Diepeningen, Anne D. Ostrowska-Kolodziejczak, Anna Krawczyk, Katarzyna Brankovics, Balázs Stenglein, Sebastian Alberto Sawicki, Jakub Perkowski, Juliusz |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
ANNOTATION CHEMOTYPES FUSARIUM MOLECULAR IDENTIFICATION TRICHOTHECENE GENOTYPES |
topic |
ANNOTATION CHEMOTYPES FUSARIUM MOLECULAR IDENTIFICATION TRICHOTHECENE GENOTYPES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.2 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Type B trichothecenes, which pose a serious hazard to consumer health, occur worldwide in grains. These mycotoxins are produced mainly by three different trichothecene genotypes/chemotypes: 3ADON (3-acetyldeoxynivalenol), 15ADON (15-acetyldeoxynivalenol) and NIV (nivalenol), named after these three major mycotoxin compounds. Correct identification of these genotypes is elementary for all studies relating to population surveys, fungal ecology and mycotoxicology. Trichothecene producers exhibit enormous strain-dependent chemical diversity, which may result in variation in levels of the genotype´s determining toxin and in the production of low to high amounts of atypical compounds. New high-throughput DNA-sequencing technologies promise to boost the diagnostics of mycotoxin genotypes. However, this requires a reference database containing a satisfactory taxonomic sampling of sequences showing high correlation to actually produced chemotypes. We believe that one of the most pressing current challenges of such a database is the linking of molecular identification with chemical diversity of the strains, as well as other metadata. In this study, we use the Tri12 gene involved in mycotoxin biosynthesis for identification of Tri genotypes through sequence comparison. Tri12 sequences from a range of geographically diverse fungal strains comprising 22 Fusarium species were stored in the ToxGen database, which covers descriptive and up-to-date annotations such as indication on Tri genotype and chemotype of the strains, chemical diversity, information on trichothecene-inducing host, substrate or media, geographical locality, and most recent taxonomic affiliations. The present initiative bridges the gap between the demands of comprehensive studies on trichothecene producers and the existing nucleotide sequence databases, which lack toxicological and other auxiliary data. We invite researchers working in the fields of fungal taxonomy, epidemiology and mycotoxicology to join the freely available annotation effort. Fil: Kulik, Tomasz. Uniwersytet Warminsko-mazurski W Olsztynie; Fil: Abarenkov, Kessy. University Of Tartu.; Estonia Fil: Busko, Maciej. Poznań University of Life Sciences; Polonia Fil: Bilska, Katarzyna. University of Warmia and Mazury; Polonia Fil: van Diepeningen, Anne D.. University of Amsterdam; Países Bajos Fil: Ostrowska-Kolodziejczak, Anna. Poznań University of Life Science; Polonia Fil: Krawczyk, Katarzyna. University of Warmia and Mazur; Polonia Fil: Brankovics, Balázs. CBS-KNAW Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países Bajos Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina Fil: Sawicki, Jakub. University of Warmia and Mazury; Polonia Fil: Perkowski, Juliusz. Poznań University of Life Sciences; Polonia |
description |
Type B trichothecenes, which pose a serious hazard to consumer health, occur worldwide in grains. These mycotoxins are produced mainly by three different trichothecene genotypes/chemotypes: 3ADON (3-acetyldeoxynivalenol), 15ADON (15-acetyldeoxynivalenol) and NIV (nivalenol), named after these three major mycotoxin compounds. Correct identification of these genotypes is elementary for all studies relating to population surveys, fungal ecology and mycotoxicology. Trichothecene producers exhibit enormous strain-dependent chemical diversity, which may result in variation in levels of the genotype´s determining toxin and in the production of low to high amounts of atypical compounds. New high-throughput DNA-sequencing technologies promise to boost the diagnostics of mycotoxin genotypes. However, this requires a reference database containing a satisfactory taxonomic sampling of sequences showing high correlation to actually produced chemotypes. We believe that one of the most pressing current challenges of such a database is the linking of molecular identification with chemical diversity of the strains, as well as other metadata. In this study, we use the Tri12 gene involved in mycotoxin biosynthesis for identification of Tri genotypes through sequence comparison. Tri12 sequences from a range of geographically diverse fungal strains comprising 22 Fusarium species were stored in the ToxGen database, which covers descriptive and up-to-date annotations such as indication on Tri genotype and chemotype of the strains, chemical diversity, information on trichothecene-inducing host, substrate or media, geographical locality, and most recent taxonomic affiliations. The present initiative bridges the gap between the demands of comprehensive studies on trichothecene producers and the existing nucleotide sequence databases, which lack toxicological and other auxiliary data. We invite researchers working in the fields of fungal taxonomy, epidemiology and mycotoxicology to join the freely available annotation effort. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/30587 Kulik, Tomasz; Abarenkov, Kessy; Busko, Maciej; Bilska, Katarzyna; van Diepeningen, Anne D.; et al.; ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium; PeerJ Inc; Peer J; 2017; 2; 2-2017; 1-19 2167-8359 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/30587 |
identifier_str_mv |
Kulik, Tomasz; Abarenkov, Kessy; Busko, Maciej; Bilska, Katarzyna; van Diepeningen, Anne D.; et al.; ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium; PeerJ Inc; Peer J; 2017; 2; 2-2017; 1-19 2167-8359 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.2992 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
PeerJ Inc |
publisher.none.fl_str_mv |
PeerJ Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269527863197696 |
score |
13.13397 |