ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium

Autores
Kulik, Tomasz; Abarenkov, Kessy; Busko, Maciej; Bilska, Katarzyna; van Diepeningen, Anne D.; Ostrowska-Kolodziejczak, Anna; Krawczyk, Katarzyna; Brankovics, Balázs; Stenglein, Sebastian Alberto; Sawicki, Jakub; Perkowski, Juliusz
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Type B trichothecenes, which pose a serious hazard to consumer health, occur worldwide in grains. These mycotoxins are produced mainly by three different trichothecene genotypes/chemotypes: 3ADON (3-acetyldeoxynivalenol), 15ADON (15-acetyldeoxynivalenol) and NIV (nivalenol), named after these three major mycotoxin compounds. Correct identification of these genotypes is elementary for all studies relating to population surveys, fungal ecology and mycotoxicology. Trichothecene producers exhibit enormous strain-dependent chemical diversity, which may result in variation in levels of the genotype´s determining toxin and in the production of low to high amounts of atypical compounds. New high-throughput DNA-sequencing technologies promise to boost the diagnostics of mycotoxin genotypes. However, this requires a reference database containing a satisfactory taxonomic sampling of sequences showing high correlation to actually produced chemotypes. We believe that one of the most pressing current challenges of such a database is the linking of molecular identification with chemical diversity of the strains, as well as other metadata. In this study, we use the Tri12 gene involved in mycotoxin biosynthesis for identification of Tri genotypes through sequence comparison. Tri12 sequences from a range of geographically diverse fungal strains comprising 22 Fusarium species were stored in the ToxGen database, which covers descriptive and up-to-date annotations such as indication on Tri genotype and chemotype of the strains, chemical diversity, information on trichothecene-inducing host, substrate or media, geographical locality, and most recent taxonomic affiliations. The present initiative bridges the gap between the demands of comprehensive studies on trichothecene producers and the existing nucleotide sequence databases, which lack toxicological and other auxiliary data. We invite researchers working in the fields of fungal taxonomy, epidemiology and mycotoxicology to join the freely available annotation effort.
Fil: Kulik, Tomasz. Uniwersytet Warminsko-mazurski W Olsztynie;
Fil: Abarenkov, Kessy. University Of Tartu.; Estonia
Fil: Busko, Maciej. Poznań University of Life Sciences; Polonia
Fil: Bilska, Katarzyna. University of Warmia and Mazury; Polonia
Fil: van Diepeningen, Anne D.. University of Amsterdam; Países Bajos
Fil: Ostrowska-Kolodziejczak, Anna. Poznań University of Life Science; Polonia
Fil: Krawczyk, Katarzyna. University of Warmia and Mazur; Polonia
Fil: Brankovics, Balázs. CBS-KNAW Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países Bajos
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Sawicki, Jakub. University of Warmia and Mazury; Polonia
Fil: Perkowski, Juliusz. Poznań University of Life Sciences; Polonia
Materia
ANNOTATION
CHEMOTYPES
FUSARIUM
MOLECULAR IDENTIFICATION
TRICHOTHECENE GENOTYPES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30587

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network_name_str CONICET Digital (CONICET)
spelling ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in FusariumKulik, TomaszAbarenkov, KessyBusko, MaciejBilska, Katarzynavan Diepeningen, Anne D.Ostrowska-Kolodziejczak, AnnaKrawczyk, KatarzynaBrankovics, BalázsStenglein, Sebastian AlbertoSawicki, JakubPerkowski, JuliuszANNOTATIONCHEMOTYPESFUSARIUMMOLECULAR IDENTIFICATIONTRICHOTHECENE GENOTYPEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.2https://purl.org/becyt/ford/1Type B trichothecenes, which pose a serious hazard to consumer health, occur worldwide in grains. These mycotoxins are produced mainly by three different trichothecene genotypes/chemotypes: 3ADON (3-acetyldeoxynivalenol), 15ADON (15-acetyldeoxynivalenol) and NIV (nivalenol), named after these three major mycotoxin compounds. Correct identification of these genotypes is elementary for all studies relating to population surveys, fungal ecology and mycotoxicology. Trichothecene producers exhibit enormous strain-dependent chemical diversity, which may result in variation in levels of the genotype´s determining toxin and in the production of low to high amounts of atypical compounds. New high-throughput DNA-sequencing technologies promise to boost the diagnostics of mycotoxin genotypes. However, this requires a reference database containing a satisfactory taxonomic sampling of sequences showing high correlation to actually produced chemotypes. We believe that one of the most pressing current challenges of such a database is the linking of molecular identification with chemical diversity of the strains, as well as other metadata. In this study, we use the Tri12 gene involved in mycotoxin biosynthesis for identification of Tri genotypes through sequence comparison. Tri12 sequences from a range of geographically diverse fungal strains comprising 22 Fusarium species were stored in the ToxGen database, which covers descriptive and up-to-date annotations such as indication on Tri genotype and chemotype of the strains, chemical diversity, information on trichothecene-inducing host, substrate or media, geographical locality, and most recent taxonomic affiliations. The present initiative bridges the gap between the demands of comprehensive studies on trichothecene producers and the existing nucleotide sequence databases, which lack toxicological and other auxiliary data. We invite researchers working in the fields of fungal taxonomy, epidemiology and mycotoxicology to join the freely available annotation effort.Fil: Kulik, Tomasz. Uniwersytet Warminsko-mazurski W Olsztynie;Fil: Abarenkov, Kessy. University Of Tartu.; EstoniaFil: Busko, Maciej. Poznań University of Life Sciences; PoloniaFil: Bilska, Katarzyna. University of Warmia and Mazury; PoloniaFil: van Diepeningen, Anne D.. University of Amsterdam; Países BajosFil: Ostrowska-Kolodziejczak, Anna. Poznań University of Life Science; PoloniaFil: Krawczyk, Katarzyna. University of Warmia and Mazur; PoloniaFil: Brankovics, Balázs. CBS-KNAW Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países BajosFil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaFil: Sawicki, Jakub. University of Warmia and Mazury; PoloniaFil: Perkowski, Juliusz. Poznań University of Life Sciences; PoloniaPeerJ Inc2017-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30587Kulik, Tomasz; Abarenkov, Kessy; Busko, Maciej; Bilska, Katarzyna; van Diepeningen, Anne D.; et al.; ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium; PeerJ Inc; Peer J; 2017; 2; 2-2017; 1-192167-8359CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.2992info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:33Zoai:ri.conicet.gov.ar:11336/30587instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:34.051CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium
title ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium
spellingShingle ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium
Kulik, Tomasz
ANNOTATION
CHEMOTYPES
FUSARIUM
MOLECULAR IDENTIFICATION
TRICHOTHECENE GENOTYPES
title_short ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium
title_full ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium
title_fullStr ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium
title_full_unstemmed ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium
title_sort ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium
dc.creator.none.fl_str_mv Kulik, Tomasz
Abarenkov, Kessy
Busko, Maciej
Bilska, Katarzyna
van Diepeningen, Anne D.
Ostrowska-Kolodziejczak, Anna
Krawczyk, Katarzyna
Brankovics, Balázs
Stenglein, Sebastian Alberto
Sawicki, Jakub
Perkowski, Juliusz
author Kulik, Tomasz
author_facet Kulik, Tomasz
Abarenkov, Kessy
Busko, Maciej
Bilska, Katarzyna
van Diepeningen, Anne D.
Ostrowska-Kolodziejczak, Anna
Krawczyk, Katarzyna
Brankovics, Balázs
Stenglein, Sebastian Alberto
Sawicki, Jakub
Perkowski, Juliusz
author_role author
author2 Abarenkov, Kessy
Busko, Maciej
Bilska, Katarzyna
van Diepeningen, Anne D.
Ostrowska-Kolodziejczak, Anna
Krawczyk, Katarzyna
Brankovics, Balázs
Stenglein, Sebastian Alberto
Sawicki, Jakub
Perkowski, Juliusz
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANNOTATION
CHEMOTYPES
FUSARIUM
MOLECULAR IDENTIFICATION
TRICHOTHECENE GENOTYPES
topic ANNOTATION
CHEMOTYPES
FUSARIUM
MOLECULAR IDENTIFICATION
TRICHOTHECENE GENOTYPES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.2
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Type B trichothecenes, which pose a serious hazard to consumer health, occur worldwide in grains. These mycotoxins are produced mainly by three different trichothecene genotypes/chemotypes: 3ADON (3-acetyldeoxynivalenol), 15ADON (15-acetyldeoxynivalenol) and NIV (nivalenol), named after these three major mycotoxin compounds. Correct identification of these genotypes is elementary for all studies relating to population surveys, fungal ecology and mycotoxicology. Trichothecene producers exhibit enormous strain-dependent chemical diversity, which may result in variation in levels of the genotype´s determining toxin and in the production of low to high amounts of atypical compounds. New high-throughput DNA-sequencing technologies promise to boost the diagnostics of mycotoxin genotypes. However, this requires a reference database containing a satisfactory taxonomic sampling of sequences showing high correlation to actually produced chemotypes. We believe that one of the most pressing current challenges of such a database is the linking of molecular identification with chemical diversity of the strains, as well as other metadata. In this study, we use the Tri12 gene involved in mycotoxin biosynthesis for identification of Tri genotypes through sequence comparison. Tri12 sequences from a range of geographically diverse fungal strains comprising 22 Fusarium species were stored in the ToxGen database, which covers descriptive and up-to-date annotations such as indication on Tri genotype and chemotype of the strains, chemical diversity, information on trichothecene-inducing host, substrate or media, geographical locality, and most recent taxonomic affiliations. The present initiative bridges the gap between the demands of comprehensive studies on trichothecene producers and the existing nucleotide sequence databases, which lack toxicological and other auxiliary data. We invite researchers working in the fields of fungal taxonomy, epidemiology and mycotoxicology to join the freely available annotation effort.
Fil: Kulik, Tomasz. Uniwersytet Warminsko-mazurski W Olsztynie;
Fil: Abarenkov, Kessy. University Of Tartu.; Estonia
Fil: Busko, Maciej. Poznań University of Life Sciences; Polonia
Fil: Bilska, Katarzyna. University of Warmia and Mazury; Polonia
Fil: van Diepeningen, Anne D.. University of Amsterdam; Países Bajos
Fil: Ostrowska-Kolodziejczak, Anna. Poznań University of Life Science; Polonia
Fil: Krawczyk, Katarzyna. University of Warmia and Mazur; Polonia
Fil: Brankovics, Balázs. CBS-KNAW Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países Bajos
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Sawicki, Jakub. University of Warmia and Mazury; Polonia
Fil: Perkowski, Juliusz. Poznań University of Life Sciences; Polonia
description Type B trichothecenes, which pose a serious hazard to consumer health, occur worldwide in grains. These mycotoxins are produced mainly by three different trichothecene genotypes/chemotypes: 3ADON (3-acetyldeoxynivalenol), 15ADON (15-acetyldeoxynivalenol) and NIV (nivalenol), named after these three major mycotoxin compounds. Correct identification of these genotypes is elementary for all studies relating to population surveys, fungal ecology and mycotoxicology. Trichothecene producers exhibit enormous strain-dependent chemical diversity, which may result in variation in levels of the genotype´s determining toxin and in the production of low to high amounts of atypical compounds. New high-throughput DNA-sequencing technologies promise to boost the diagnostics of mycotoxin genotypes. However, this requires a reference database containing a satisfactory taxonomic sampling of sequences showing high correlation to actually produced chemotypes. We believe that one of the most pressing current challenges of such a database is the linking of molecular identification with chemical diversity of the strains, as well as other metadata. In this study, we use the Tri12 gene involved in mycotoxin biosynthesis for identification of Tri genotypes through sequence comparison. Tri12 sequences from a range of geographically diverse fungal strains comprising 22 Fusarium species were stored in the ToxGen database, which covers descriptive and up-to-date annotations such as indication on Tri genotype and chemotype of the strains, chemical diversity, information on trichothecene-inducing host, substrate or media, geographical locality, and most recent taxonomic affiliations. The present initiative bridges the gap between the demands of comprehensive studies on trichothecene producers and the existing nucleotide sequence databases, which lack toxicological and other auxiliary data. We invite researchers working in the fields of fungal taxonomy, epidemiology and mycotoxicology to join the freely available annotation effort.
publishDate 2017
dc.date.none.fl_str_mv 2017-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30587
Kulik, Tomasz; Abarenkov, Kessy; Busko, Maciej; Bilska, Katarzyna; van Diepeningen, Anne D.; et al.; ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium; PeerJ Inc; Peer J; 2017; 2; 2-2017; 1-19
2167-8359
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30587
identifier_str_mv Kulik, Tomasz; Abarenkov, Kessy; Busko, Maciej; Bilska, Katarzyna; van Diepeningen, Anne D.; et al.; ToxGen: An improved reference database for the identification of type B-trichothecene genotypes in Fusarium; PeerJ Inc; Peer J; 2017; 2; 2-2017; 1-19
2167-8359
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.2992
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv PeerJ Inc
publisher.none.fl_str_mv PeerJ Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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