Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina

Autores
Kulik, Tomasz; Busko, Maciej; Bilska, Katarzyna; Ostrowska-Kołodziejczak, Anna; van Diepeningen, Anne D.; Perkowski, Juliusz; Stenglein, Sebastian Alberto
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between trichothecene genotype and chemotype might result from inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.
Fil: Kulik, Tomasz. University of Warmia and Mazury; Polonia
Fil: Busko, Maciej. Poznan University of Life Sciences; Polonia
Fil: Bilska, Katarzyna. University of Warmia and Mazury; Polonia
Fil: Ostrowska-Kołodziejczak, Anna. Poznan University of Life Sciences; Polonia
Fil: van Diepeningen, Anne D.. Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países Bajos
Fil: Perkowski, Juliusz. Poznan University of Life Sciences; Polonia
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina
Materia
FUSARIUM
TRI GENOTYPES
CHEMOTYPES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/27073
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from ArgentinaKulik, TomaszBusko, MaciejBilska, KatarzynaOstrowska-Kołodziejczak, Annavan Diepeningen, Anne D.Perkowski, JuliuszStenglein, Sebastian AlbertoFUSARIUMTRI GENOTYPESCHEMOTYPEShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between trichothecene genotype and chemotype might result from inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.Fil: Kulik, Tomasz. University of Warmia and Mazury; PoloniaFil: Busko, Maciej. Poznan University of Life Sciences; PoloniaFil: Bilska, Katarzyna. University of Warmia and Mazury; PoloniaFil: Ostrowska-Kołodziejczak, Anna. Poznan University of Life Sciences; PoloniaFil: van Diepeningen, Anne D.. Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países BajosFil: Perkowski, Juliusz. Poznan University of Life Sciences; PoloniaFil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaMolecular Diversity Preservation International2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27073Kulik, Tomasz; Busko, Maciej; Bilska, Katarzyna; Ostrowska-Kołodziejczak, Anna; van Diepeningen, Anne D.; et al.; Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina; Molecular Diversity Preservation International; Toxins; 8; 11; 11-2016; 330-3372072-6651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/toxins8110330info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2072-6651/8/11/330info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:53Zoai:ri.conicet.gov.ar:11336/27073instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:53.51CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina
title Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina
spellingShingle Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina
Kulik, Tomasz
FUSARIUM
TRI GENOTYPES
CHEMOTYPES
title_short Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina
title_full Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina
title_fullStr Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina
title_full_unstemmed Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina
title_sort Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina
dc.creator.none.fl_str_mv Kulik, Tomasz
Busko, Maciej
Bilska, Katarzyna
Ostrowska-Kołodziejczak, Anna
van Diepeningen, Anne D.
Perkowski, Juliusz
Stenglein, Sebastian Alberto
author Kulik, Tomasz
author_facet Kulik, Tomasz
Busko, Maciej
Bilska, Katarzyna
Ostrowska-Kołodziejczak, Anna
van Diepeningen, Anne D.
Perkowski, Juliusz
Stenglein, Sebastian Alberto
author_role author
author2 Busko, Maciej
Bilska, Katarzyna
Ostrowska-Kołodziejczak, Anna
van Diepeningen, Anne D.
Perkowski, Juliusz
Stenglein, Sebastian Alberto
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv FUSARIUM
TRI GENOTYPES
CHEMOTYPES
topic FUSARIUM
TRI GENOTYPES
CHEMOTYPES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between trichothecene genotype and chemotype might result from inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.
Fil: Kulik, Tomasz. University of Warmia and Mazury; Polonia
Fil: Busko, Maciej. Poznan University of Life Sciences; Polonia
Fil: Bilska, Katarzyna. University of Warmia and Mazury; Polonia
Fil: Ostrowska-Kołodziejczak, Anna. Poznan University of Life Sciences; Polonia
Fil: van Diepeningen, Anne D.. Fungal Biodiversity Centre; Países Bajos. University of Amsterdam; Países Bajos
Fil: Perkowski, Juliusz. Poznan University of Life Sciences; Polonia
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina
description Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between trichothecene genotype and chemotype might result from inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/27073
Kulik, Tomasz; Busko, Maciej; Bilska, Katarzyna; Ostrowska-Kołodziejczak, Anna; van Diepeningen, Anne D.; et al.; Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina; Molecular Diversity Preservation International; Toxins; 8; 11; 11-2016; 330-337
2072-6651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/27073
identifier_str_mv Kulik, Tomasz; Busko, Maciej; Bilska, Katarzyna; Ostrowska-Kołodziejczak, Anna; van Diepeningen, Anne D.; et al.; Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina; Molecular Diversity Preservation International; Toxins; 8; 11; 11-2016; 330-337
2072-6651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/toxins8110330
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2072-6651/8/11/330
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269548504416256
score 13.13397

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