Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-...

Autores
Yerkovich, Nadia; Palazzini, Juan Manuel; Sulyok, Michael; Chulze, Sofia Noemi
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Members of Fusarium graminearum species complex are the main pathogens associated with Fusarium head blight (FHB) in wheat in Argentina, which produce trichothecene mycotoxins that are found in wheat grain and by-products. The aim of this study was to determine the chemotype and trichothecene genotypes of Fusarium graminearum species complex strains isolated from wheat in Argentina during an epidemic and a non-epidemic harvest season. A total of 115 and 108 strains obtained from wheat during 2012/13 and 2014/15 harvest seasons, respectively, were identified as belonging to the Fusarium graminearum species complex. PCR assays were used to differentiate the 15-acetyldeoxynivalenol (15-ADON), 3-acetyldeoxynivalenol (3-ADON) and nivalenol (NIV) genotypes. The trichothecene type B chemotype, zearalenone, and NX-2 toxin profiles were determined based on chromatographic (LC-MS/MS) analysis. Differences in the genotype frequency were due to the target locus analyzed and year of isolation of the strain. The most common genotype and chemotype was 15-ADON in both seasons, but an increasing frequency of the 3-ADON chemotype was found in the non-epidemic season (2014/15). The strains were able to produce zearalenone and its masked derivatives, but not the type A trichothecene NX-2. This is the first report of the production of zearalenone and its modified mycotoxins by Fusarium graminearum species complex strains isolated from Argentina.
Fil: Yerkovich, Nadia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Palazzini, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Sulyok, Michael. Universitat Fur Bodenkultur Wien; Austria
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
Chemotype
Fusarium
Genotype
Mycotoxins
Triticum Aestivum L
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/74569
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/74569
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic seasonYerkovich, NadiaPalazzini, Juan ManuelSulyok, MichaelChulze, Sofia NoemiChemotypeFusariumGenotypeMycotoxinsTriticum Aestivum Lhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Members of Fusarium graminearum species complex are the main pathogens associated with Fusarium head blight (FHB) in wheat in Argentina, which produce trichothecene mycotoxins that are found in wheat grain and by-products. The aim of this study was to determine the chemotype and trichothecene genotypes of Fusarium graminearum species complex strains isolated from wheat in Argentina during an epidemic and a non-epidemic harvest season. A total of 115 and 108 strains obtained from wheat during 2012/13 and 2014/15 harvest seasons, respectively, were identified as belonging to the Fusarium graminearum species complex. PCR assays were used to differentiate the 15-acetyldeoxynivalenol (15-ADON), 3-acetyldeoxynivalenol (3-ADON) and nivalenol (NIV) genotypes. The trichothecene type B chemotype, zearalenone, and NX-2 toxin profiles were determined based on chromatographic (LC-MS/MS) analysis. Differences in the genotype frequency were due to the target locus analyzed and year of isolation of the strain. The most common genotype and chemotype was 15-ADON in both seasons, but an increasing frequency of the 3-ADON chemotype was found in the non-epidemic season (2014/15). The strains were able to produce zearalenone and its masked derivatives, but not the type A trichothecene NX-2. This is the first report of the production of zearalenone and its modified mycotoxins by Fusarium graminearum species complex strains isolated from Argentina.Fil: Yerkovich, Nadia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Palazzini, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Sulyok, Michael. Universitat Fur Bodenkultur Wien; AustriaFil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaSociedade Brasileira de Fitopatologia2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/74569Yerkovich, Nadia; Palazzini, Juan Manuel; Sulyok, Michael; Chulze, Sofia Noemi; Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season; Sociedade Brasileira de Fitopatologia; Tropical Plant Pathology; 42; 3; 6-2017; 190-1961982-56761983-2052CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s40858-017-0155-3info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs40858-017-0155-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:52Zoai:ri.conicet.gov.ar:11336/74569instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:53.025CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
title Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
spellingShingle Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
Yerkovich, Nadia
Chemotype
Fusarium
Genotype
Mycotoxins
Triticum Aestivum L
title_short Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
title_full Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
title_fullStr Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
title_full_unstemmed Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
title_sort Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
dc.creator.none.fl_str_mv Yerkovich, Nadia
Palazzini, Juan Manuel
Sulyok, Michael
Chulze, Sofia Noemi
author Yerkovich, Nadia
author_facet Yerkovich, Nadia
Palazzini, Juan Manuel
Sulyok, Michael
Chulze, Sofia Noemi
author_role author
author2 Palazzini, Juan Manuel
Sulyok, Michael
Chulze, Sofia Noemi
author2_role author
author
author
dc.subject.none.fl_str_mv Chemotype
Fusarium
Genotype
Mycotoxins
Triticum Aestivum L
topic Chemotype
Fusarium
Genotype
Mycotoxins
Triticum Aestivum L
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Members of Fusarium graminearum species complex are the main pathogens associated with Fusarium head blight (FHB) in wheat in Argentina, which produce trichothecene mycotoxins that are found in wheat grain and by-products. The aim of this study was to determine the chemotype and trichothecene genotypes of Fusarium graminearum species complex strains isolated from wheat in Argentina during an epidemic and a non-epidemic harvest season. A total of 115 and 108 strains obtained from wheat during 2012/13 and 2014/15 harvest seasons, respectively, were identified as belonging to the Fusarium graminearum species complex. PCR assays were used to differentiate the 15-acetyldeoxynivalenol (15-ADON), 3-acetyldeoxynivalenol (3-ADON) and nivalenol (NIV) genotypes. The trichothecene type B chemotype, zearalenone, and NX-2 toxin profiles were determined based on chromatographic (LC-MS/MS) analysis. Differences in the genotype frequency were due to the target locus analyzed and year of isolation of the strain. The most common genotype and chemotype was 15-ADON in both seasons, but an increasing frequency of the 3-ADON chemotype was found in the non-epidemic season (2014/15). The strains were able to produce zearalenone and its masked derivatives, but not the type A trichothecene NX-2. This is the first report of the production of zearalenone and its modified mycotoxins by Fusarium graminearum species complex strains isolated from Argentina.
Fil: Yerkovich, Nadia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Palazzini, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Sulyok, Michael. Universitat Fur Bodenkultur Wien; Austria
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description Members of Fusarium graminearum species complex are the main pathogens associated with Fusarium head blight (FHB) in wheat in Argentina, which produce trichothecene mycotoxins that are found in wheat grain and by-products. The aim of this study was to determine the chemotype and trichothecene genotypes of Fusarium graminearum species complex strains isolated from wheat in Argentina during an epidemic and a non-epidemic harvest season. A total of 115 and 108 strains obtained from wheat during 2012/13 and 2014/15 harvest seasons, respectively, were identified as belonging to the Fusarium graminearum species complex. PCR assays were used to differentiate the 15-acetyldeoxynivalenol (15-ADON), 3-acetyldeoxynivalenol (3-ADON) and nivalenol (NIV) genotypes. The trichothecene type B chemotype, zearalenone, and NX-2 toxin profiles were determined based on chromatographic (LC-MS/MS) analysis. Differences in the genotype frequency were due to the target locus analyzed and year of isolation of the strain. The most common genotype and chemotype was 15-ADON in both seasons, but an increasing frequency of the 3-ADON chemotype was found in the non-epidemic season (2014/15). The strains were able to produce zearalenone and its masked derivatives, but not the type A trichothecene NX-2. This is the first report of the production of zearalenone and its modified mycotoxins by Fusarium graminearum species complex strains isolated from Argentina.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/74569
Yerkovich, Nadia; Palazzini, Juan Manuel; Sulyok, Michael; Chulze, Sofia Noemi; Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season; Sociedade Brasileira de Fitopatologia; Tropical Plant Pathology; 42; 3; 6-2017; 190-196
1982-5676
1983-2052
CONICET Digital
CONICET
url http://hdl.handle.net/11336/74569
identifier_str_mv Yerkovich, Nadia; Palazzini, Juan Manuel; Sulyok, Michael; Chulze, Sofia Noemi; Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season; Sociedade Brasileira de Fitopatologia; Tropical Plant Pathology; 42; 3; 6-2017; 190-196
1982-5676
1983-2052
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s40858-017-0155-3
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs40858-017-0155-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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