Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes
- Autores
- Navarrete Zamora, Miluska Beatriz; Acuña, Francisco; Duarte da Silva, Mônica; Santos Silva, Thamires; Garcia, Matheus Henrique Herminio; Barreto, Rodrigo da Silva Nunes; Sato Sato, Alberto; Barbeito, Claudio Gustavo; Miglino, Maria Angelica
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- South American camelids inhabit high-altitude environments characterized by hypoxia, influencing embryonic, fetal, and placental development. This study examined the term placenta morphology of alpacas (Vicugna pacos, N = 12) and the immunoexpression of antioxidant selenoproteins (SP). We hypothesize that the placenta of alpacas, adapted to high altitudes, has characteristics with other species also adapted to altitude. Placentas were paraffin-embedded, sectioned (3-5 µm), stained with hematoxylin-eosin (H&E), Masson´s trichrome, and picrosirius red, and analyzed via light and polarized light microscopy. The chorion showed simple cuboidal epithelium with binucleated cells, a subepithelial mesenchyme rich in blood capillaries (area: 124.90 ± 9.82 µm2), and type III collagen fibers. The chorionic villi measured 2740.22 ± 132.75 µm. The allantois contained a simple columnar epithelium and mesenchyme with type I collagen fibers. Immunohistochemistry localized SP-N, SP-P, Dio-3, and GPx-3 in the blood capillaries and mesenchymal tissue of the chorion but not in the allantois. These findings were compared to human and sheep placentas from different altitudes due to a lack of camelid data at low levels. The morphological features resembled adaptations to hypoxia observed in other species. This preliminary study suggests a potential role for selenoproteins in hypoxia adaptation, providing a basis for future functional studies.
Fil: Navarrete Zamora, Miluska Beatriz. Universidad Nacional Mayor de San Marcos; Perú
Fil: Acuña, Francisco. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Duarte da Silva, Mônica. Universidade de Sao Paulo; Brasil
Fil: Santos Silva, Thamires. Universidade de Sao Paulo; Brasil
Fil: Garcia, Matheus Henrique Herminio. Universidade de Sao Paulo; Brasil
Fil: Barreto, Rodrigo da Silva Nunes. Universidade de Sao Paulo; Brasil
Fil: Sato Sato, Alberto. Universidade de Sao Paulo; Brasil
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Miglino, Maria Angelica. Universidade de Sao Paulo; Brasil - Materia
-
Camelids
Extreme environments
Fetus;
Placenta
Selenium - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/267078
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/267078 |
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CONICET Digital (CONICET) |
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Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian AndesNavarrete Zamora, Miluska BeatrizAcuña, FranciscoDuarte da Silva, MônicaSantos Silva, ThamiresGarcia, Matheus Henrique HerminioBarreto, Rodrigo da Silva NunesSato Sato, AlbertoBarbeito, Claudio GustavoMiglino, Maria AngelicaCamelidsExtreme environmentsFetus;PlacentaSeleniumhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1South American camelids inhabit high-altitude environments characterized by hypoxia, influencing embryonic, fetal, and placental development. This study examined the term placenta morphology of alpacas (Vicugna pacos, N = 12) and the immunoexpression of antioxidant selenoproteins (SP). We hypothesize that the placenta of alpacas, adapted to high altitudes, has characteristics with other species also adapted to altitude. Placentas were paraffin-embedded, sectioned (3-5 µm), stained with hematoxylin-eosin (H&E), Masson´s trichrome, and picrosirius red, and analyzed via light and polarized light microscopy. The chorion showed simple cuboidal epithelium with binucleated cells, a subepithelial mesenchyme rich in blood capillaries (area: 124.90 ± 9.82 µm2), and type III collagen fibers. The chorionic villi measured 2740.22 ± 132.75 µm. The allantois contained a simple columnar epithelium and mesenchyme with type I collagen fibers. Immunohistochemistry localized SP-N, SP-P, Dio-3, and GPx-3 in the blood capillaries and mesenchymal tissue of the chorion but not in the allantois. These findings were compared to human and sheep placentas from different altitudes due to a lack of camelid data at low levels. The morphological features resembled adaptations to hypoxia observed in other species. This preliminary study suggests a potential role for selenoproteins in hypoxia adaptation, providing a basis for future functional studies.Fil: Navarrete Zamora, Miluska Beatriz. Universidad Nacional Mayor de San Marcos; PerúFil: Acuña, Francisco. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Duarte da Silva, Mônica. Universidade de Sao Paulo; BrasilFil: Santos Silva, Thamires. Universidade de Sao Paulo; BrasilFil: Garcia, Matheus Henrique Herminio. Universidade de Sao Paulo; BrasilFil: Barreto, Rodrigo da Silva Nunes. Universidade de Sao Paulo; BrasilFil: Sato Sato, Alberto. Universidade de Sao Paulo; BrasilFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Miglino, Maria Angelica. Universidade de Sao Paulo; BrasilMDPI2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267078Navarrete Zamora, Miluska Beatriz; Acuña, Francisco; Duarte da Silva, Mônica; Santos Silva, Thamires; Garcia, Matheus Henrique Herminio; et al.; Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes; MDPI; Biology; 14; 1; 1-2025; 1-122079-7737CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/biology14010064info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:52Zoai:ri.conicet.gov.ar:11336/267078instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:52.243CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes |
title |
Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes |
spellingShingle |
Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes Navarrete Zamora, Miluska Beatriz Camelids Extreme environments Fetus; Placenta Selenium |
title_short |
Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes |
title_full |
Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes |
title_fullStr |
Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes |
title_full_unstemmed |
Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes |
title_sort |
Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes |
dc.creator.none.fl_str_mv |
Navarrete Zamora, Miluska Beatriz Acuña, Francisco Duarte da Silva, Mônica Santos Silva, Thamires Garcia, Matheus Henrique Herminio Barreto, Rodrigo da Silva Nunes Sato Sato, Alberto Barbeito, Claudio Gustavo Miglino, Maria Angelica |
author |
Navarrete Zamora, Miluska Beatriz |
author_facet |
Navarrete Zamora, Miluska Beatriz Acuña, Francisco Duarte da Silva, Mônica Santos Silva, Thamires Garcia, Matheus Henrique Herminio Barreto, Rodrigo da Silva Nunes Sato Sato, Alberto Barbeito, Claudio Gustavo Miglino, Maria Angelica |
author_role |
author |
author2 |
Acuña, Francisco Duarte da Silva, Mônica Santos Silva, Thamires Garcia, Matheus Henrique Herminio Barreto, Rodrigo da Silva Nunes Sato Sato, Alberto Barbeito, Claudio Gustavo Miglino, Maria Angelica |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Camelids Extreme environments Fetus; Placenta Selenium |
topic |
Camelids Extreme environments Fetus; Placenta Selenium |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
South American camelids inhabit high-altitude environments characterized by hypoxia, influencing embryonic, fetal, and placental development. This study examined the term placenta morphology of alpacas (Vicugna pacos, N = 12) and the immunoexpression of antioxidant selenoproteins (SP). We hypothesize that the placenta of alpacas, adapted to high altitudes, has characteristics with other species also adapted to altitude. Placentas were paraffin-embedded, sectioned (3-5 µm), stained with hematoxylin-eosin (H&E), Masson´s trichrome, and picrosirius red, and analyzed via light and polarized light microscopy. The chorion showed simple cuboidal epithelium with binucleated cells, a subepithelial mesenchyme rich in blood capillaries (area: 124.90 ± 9.82 µm2), and type III collagen fibers. The chorionic villi measured 2740.22 ± 132.75 µm. The allantois contained a simple columnar epithelium and mesenchyme with type I collagen fibers. Immunohistochemistry localized SP-N, SP-P, Dio-3, and GPx-3 in the blood capillaries and mesenchymal tissue of the chorion but not in the allantois. These findings were compared to human and sheep placentas from different altitudes due to a lack of camelid data at low levels. The morphological features resembled adaptations to hypoxia observed in other species. This preliminary study suggests a potential role for selenoproteins in hypoxia adaptation, providing a basis for future functional studies. Fil: Navarrete Zamora, Miluska Beatriz. Universidad Nacional Mayor de San Marcos; Perú Fil: Acuña, Francisco. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Duarte da Silva, Mônica. Universidade de Sao Paulo; Brasil Fil: Santos Silva, Thamires. Universidade de Sao Paulo; Brasil Fil: Garcia, Matheus Henrique Herminio. Universidade de Sao Paulo; Brasil Fil: Barreto, Rodrigo da Silva Nunes. Universidade de Sao Paulo; Brasil Fil: Sato Sato, Alberto. Universidade de Sao Paulo; Brasil Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina Fil: Miglino, Maria Angelica. Universidade de Sao Paulo; Brasil |
description |
South American camelids inhabit high-altitude environments characterized by hypoxia, influencing embryonic, fetal, and placental development. This study examined the term placenta morphology of alpacas (Vicugna pacos, N = 12) and the immunoexpression of antioxidant selenoproteins (SP). We hypothesize that the placenta of alpacas, adapted to high altitudes, has characteristics with other species also adapted to altitude. Placentas were paraffin-embedded, sectioned (3-5 µm), stained with hematoxylin-eosin (H&E), Masson´s trichrome, and picrosirius red, and analyzed via light and polarized light microscopy. The chorion showed simple cuboidal epithelium with binucleated cells, a subepithelial mesenchyme rich in blood capillaries (area: 124.90 ± 9.82 µm2), and type III collagen fibers. The chorionic villi measured 2740.22 ± 132.75 µm. The allantois contained a simple columnar epithelium and mesenchyme with type I collagen fibers. Immunohistochemistry localized SP-N, SP-P, Dio-3, and GPx-3 in the blood capillaries and mesenchymal tissue of the chorion but not in the allantois. These findings were compared to human and sheep placentas from different altitudes due to a lack of camelid data at low levels. The morphological features resembled adaptations to hypoxia observed in other species. This preliminary study suggests a potential role for selenoproteins in hypoxia adaptation, providing a basis for future functional studies. |
publishDate |
2025 |
dc.date.none.fl_str_mv |
2025-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/267078 Navarrete Zamora, Miluska Beatriz; Acuña, Francisco; Duarte da Silva, Mônica; Santos Silva, Thamires; Garcia, Matheus Henrique Herminio; et al.; Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes; MDPI; Biology; 14; 1; 1-2025; 1-12 2079-7737 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/267078 |
identifier_str_mv |
Navarrete Zamora, Miluska Beatriz; Acuña, Francisco; Duarte da Silva, Mônica; Santos Silva, Thamires; Garcia, Matheus Henrique Herminio; et al.; Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes; MDPI; Biology; 14; 1; 1-2025; 1-12 2079-7737 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.3390/biology14010064 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269774436892672 |
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13.13397 |