Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes

Autores
Navarrete Zamora, Miluska Beatriz; Acuña, Francisco; Duarte da Silva, Mônica; Santos Silva, Thamires; Garcia, Matheus Henrique Herminio; Barreto, Rodrigo da Silva Nunes; Sato Sato, Alberto; Barbeito, Claudio Gustavo; Miglino, Maria Angelica
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
South American camelids inhabit high-altitude environments characterized by hypoxia, influencing embryonic, fetal, and placental development. This study examined the term placenta morphology of alpacas (Vicugna pacos, N = 12) and the immunoexpression of antioxidant selenoproteins (SP). We hypothesize that the placenta of alpacas, adapted to high altitudes, has characteristics with other species also adapted to altitude. Placentas were paraffin-embedded, sectioned (3-5 µm), stained with hematoxylin-eosin (H&E), Masson´s trichrome, and picrosirius red, and analyzed via light and polarized light microscopy. The chorion showed simple cuboidal epithelium with binucleated cells, a subepithelial mesenchyme rich in blood capillaries (area: 124.90 ± 9.82 µm2), and type III collagen fibers. The chorionic villi measured 2740.22 ± 132.75 µm. The allantois contained a simple columnar epithelium and mesenchyme with type I collagen fibers. Immunohistochemistry localized SP-N, SP-P, Dio-3, and GPx-3 in the blood capillaries and mesenchymal tissue of the chorion but not in the allantois. These findings were compared to human and sheep placentas from different altitudes due to a lack of camelid data at low levels. The morphological features resembled adaptations to hypoxia observed in other species. This preliminary study suggests a potential role for selenoproteins in hypoxia adaptation, providing a basis for future functional studies.
Fil: Navarrete Zamora, Miluska Beatriz. Universidad Nacional Mayor de San Marcos; Perú
Fil: Acuña, Francisco. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Duarte da Silva, Mônica. Universidade de Sao Paulo; Brasil
Fil: Santos Silva, Thamires. Universidade de Sao Paulo; Brasil
Fil: Garcia, Matheus Henrique Herminio. Universidade de Sao Paulo; Brasil
Fil: Barreto, Rodrigo da Silva Nunes. Universidade de Sao Paulo; Brasil
Fil: Sato Sato, Alberto. Universidade de Sao Paulo; Brasil
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Miglino, Maria Angelica. Universidade de Sao Paulo; Brasil
Materia
Camelids
Extreme environments
Fetus;
Placenta
Selenium
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/267078
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/267078
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian AndesNavarrete Zamora, Miluska BeatrizAcuña, FranciscoDuarte da Silva, MônicaSantos Silva, ThamiresGarcia, Matheus Henrique HerminioBarreto, Rodrigo da Silva NunesSato Sato, AlbertoBarbeito, Claudio GustavoMiglino, Maria AngelicaCamelidsExtreme environmentsFetus;PlacentaSeleniumhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1South American camelids inhabit high-altitude environments characterized by hypoxia, influencing embryonic, fetal, and placental development. This study examined the term placenta morphology of alpacas (Vicugna pacos, N = 12) and the immunoexpression of antioxidant selenoproteins (SP). We hypothesize that the placenta of alpacas, adapted to high altitudes, has characteristics with other species also adapted to altitude. Placentas were paraffin-embedded, sectioned (3-5 µm), stained with hematoxylin-eosin (H&E), Masson´s trichrome, and picrosirius red, and analyzed via light and polarized light microscopy. The chorion showed simple cuboidal epithelium with binucleated cells, a subepithelial mesenchyme rich in blood capillaries (area: 124.90 ± 9.82 µm2), and type III collagen fibers. The chorionic villi measured 2740.22 ± 132.75 µm. The allantois contained a simple columnar epithelium and mesenchyme with type I collagen fibers. Immunohistochemistry localized SP-N, SP-P, Dio-3, and GPx-3 in the blood capillaries and mesenchymal tissue of the chorion but not in the allantois. These findings were compared to human and sheep placentas from different altitudes due to a lack of camelid data at low levels. The morphological features resembled adaptations to hypoxia observed in other species. This preliminary study suggests a potential role for selenoproteins in hypoxia adaptation, providing a basis for future functional studies.Fil: Navarrete Zamora, Miluska Beatriz. Universidad Nacional Mayor de San Marcos; PerúFil: Acuña, Francisco. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Duarte da Silva, Mônica. Universidade de Sao Paulo; BrasilFil: Santos Silva, Thamires. Universidade de Sao Paulo; BrasilFil: Garcia, Matheus Henrique Herminio. Universidade de Sao Paulo; BrasilFil: Barreto, Rodrigo da Silva Nunes. Universidade de Sao Paulo; BrasilFil: Sato Sato, Alberto. Universidade de Sao Paulo; BrasilFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Miglino, Maria Angelica. Universidade de Sao Paulo; BrasilMDPI2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267078Navarrete Zamora, Miluska Beatriz; Acuña, Francisco; Duarte da Silva, Mônica; Santos Silva, Thamires; Garcia, Matheus Henrique Herminio; et al.; Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes; MDPI; Biology; 14; 1; 1-2025; 1-122079-7737CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/biology14010064info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:11Zoai:ri.conicet.gov.ar:11336/267078instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:11.29CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes
title Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes
spellingShingle Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes
Navarrete Zamora, Miluska Beatriz
Camelids
Extreme environments
Fetus;
Placenta
Selenium
title_short Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes
title_full Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes
title_fullStr Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes
title_full_unstemmed Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes
title_sort Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes
dc.creator.none.fl_str_mv Navarrete Zamora, Miluska Beatriz
Acuña, Francisco
Duarte da Silva, Mônica
Santos Silva, Thamires
Garcia, Matheus Henrique Herminio
Barreto, Rodrigo da Silva Nunes
Sato Sato, Alberto
Barbeito, Claudio Gustavo
Miglino, Maria Angelica
author Navarrete Zamora, Miluska Beatriz
author_facet Navarrete Zamora, Miluska Beatriz
Acuña, Francisco
Duarte da Silva, Mônica
Santos Silva, Thamires
Garcia, Matheus Henrique Herminio
Barreto, Rodrigo da Silva Nunes
Sato Sato, Alberto
Barbeito, Claudio Gustavo
Miglino, Maria Angelica
author_role author
author2 Acuña, Francisco
Duarte da Silva, Mônica
Santos Silva, Thamires
Garcia, Matheus Henrique Herminio
Barreto, Rodrigo da Silva Nunes
Sato Sato, Alberto
Barbeito, Claudio Gustavo
Miglino, Maria Angelica
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Camelids
Extreme environments
Fetus;
Placenta
Selenium
topic Camelids
Extreme environments
Fetus;
Placenta
Selenium
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv South American camelids inhabit high-altitude environments characterized by hypoxia, influencing embryonic, fetal, and placental development. This study examined the term placenta morphology of alpacas (Vicugna pacos, N = 12) and the immunoexpression of antioxidant selenoproteins (SP). We hypothesize that the placenta of alpacas, adapted to high altitudes, has characteristics with other species also adapted to altitude. Placentas were paraffin-embedded, sectioned (3-5 µm), stained with hematoxylin-eosin (H&E), Masson´s trichrome, and picrosirius red, and analyzed via light and polarized light microscopy. The chorion showed simple cuboidal epithelium with binucleated cells, a subepithelial mesenchyme rich in blood capillaries (area: 124.90 ± 9.82 µm2), and type III collagen fibers. The chorionic villi measured 2740.22 ± 132.75 µm. The allantois contained a simple columnar epithelium and mesenchyme with type I collagen fibers. Immunohistochemistry localized SP-N, SP-P, Dio-3, and GPx-3 in the blood capillaries and mesenchymal tissue of the chorion but not in the allantois. These findings were compared to human and sheep placentas from different altitudes due to a lack of camelid data at low levels. The morphological features resembled adaptations to hypoxia observed in other species. This preliminary study suggests a potential role for selenoproteins in hypoxia adaptation, providing a basis for future functional studies.
Fil: Navarrete Zamora, Miluska Beatriz. Universidad Nacional Mayor de San Marcos; Perú
Fil: Acuña, Francisco. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Duarte da Silva, Mônica. Universidade de Sao Paulo; Brasil
Fil: Santos Silva, Thamires. Universidade de Sao Paulo; Brasil
Fil: Garcia, Matheus Henrique Herminio. Universidade de Sao Paulo; Brasil
Fil: Barreto, Rodrigo da Silva Nunes. Universidade de Sao Paulo; Brasil
Fil: Sato Sato, Alberto. Universidade de Sao Paulo; Brasil
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Miglino, Maria Angelica. Universidade de Sao Paulo; Brasil
description South American camelids inhabit high-altitude environments characterized by hypoxia, influencing embryonic, fetal, and placental development. This study examined the term placenta morphology of alpacas (Vicugna pacos, N = 12) and the immunoexpression of antioxidant selenoproteins (SP). We hypothesize that the placenta of alpacas, adapted to high altitudes, has characteristics with other species also adapted to altitude. Placentas were paraffin-embedded, sectioned (3-5 µm), stained with hematoxylin-eosin (H&E), Masson´s trichrome, and picrosirius red, and analyzed via light and polarized light microscopy. The chorion showed simple cuboidal epithelium with binucleated cells, a subepithelial mesenchyme rich in blood capillaries (area: 124.90 ± 9.82 µm2), and type III collagen fibers. The chorionic villi measured 2740.22 ± 132.75 µm. The allantois contained a simple columnar epithelium and mesenchyme with type I collagen fibers. Immunohistochemistry localized SP-N, SP-P, Dio-3, and GPx-3 in the blood capillaries and mesenchymal tissue of the chorion but not in the allantois. These findings were compared to human and sheep placentas from different altitudes due to a lack of camelid data at low levels. The morphological features resembled adaptations to hypoxia observed in other species. This preliminary study suggests a potential role for selenoproteins in hypoxia adaptation, providing a basis for future functional studies.
publishDate 2025
dc.date.none.fl_str_mv 2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/267078
Navarrete Zamora, Miluska Beatriz; Acuña, Francisco; Duarte da Silva, Mônica; Santos Silva, Thamires; Garcia, Matheus Henrique Herminio; et al.; Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes; MDPI; Biology; 14; 1; 1-2025; 1-12
2079-7737
CONICET Digital
CONICET
url http://hdl.handle.net/11336/267078
identifier_str_mv Navarrete Zamora, Miluska Beatriz; Acuña, Francisco; Duarte da Silva, Mônica; Santos Silva, Thamires; Garcia, Matheus Henrique Herminio; et al.; Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (Vicugna pacos) from the Peruvian Andes; MDPI; Biology; 14; 1; 1-2025; 1-12
2079-7737
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/biology14010064
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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