Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution

Autores
Gómez Quispe, O. E.; Gutiérrez Reynoso, G. A.; Gallegos Cardenas, A.; Fumuso, Fernanda Gabriela; Asparrin, M.; Asparrin Del Carpio, M.; Jara, C. W.; Ponce, D.; Miguel, M.; Youngs, C. R.; Vivanco, H. W.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. Aims: The objective of this investigation was to evaluate recovery, motility, and plasma membrane integrity of alpaca spermatozoa after centrifugation in one of two different solutions at one of three different combinations of speed and time. Methods: A total of 24 ejaculates was recovered from seven reproductively sound Huacaya males using a modified artificial vagina (AV) after training the animals for semen collection. A 2 × 3 factorial treatment arrangement was utilized for this study. Ejaculates were divided into fractions for centrifugation in one of two solutions (Tris extender or PureSperm®80 density gradient solution) at one of three combinations of speed and time (492 × g for 15 min, 1968 × g for 10 min, or 4448 × g for 7 min). The experiment was replicated eight times. Results: Analysis revealed that centrifugation at 4448 × g for 7 min in PureSperm®80 provided a high recovery rate of spermatozoa with the highest sperm motility and functional integrity of plasma membrane post-centrifugation. Conclusion: Results suggest that adoption of this procedure (centrifugation at 4448 × g for 7 min in PureSperm®80) in the initial processing of alpaca ejaculates may enhance subsequent ability to use semen for AI and other assisted reproductive biotechnologies in this species
Fil: Gómez Quispe, O. E.. Universidad Nacional Agraria La Molina; Perú. Universidad Nacional Micaela Bastidas de Apurímac; Perú
Fil: Gutiérrez Reynoso, G. A.. Universidad Nacional Agraria La Molina; Perú
Fil: Gallegos Cardenas, A.. Universidad Nacional Agraria La Molina; Perú
Fil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asparrin, M.. Fundo Mallkini de Michael & Cia S.A.; Perú
Fil: Asparrin Del Carpio, M.. Fundo Mallkini de Michael & Cia S.A.; Perú
Fil: Jara, C. W.. Fundo Mallkini de Michael & Cia S.A.; Perú
Fil: Ponce, D.. Vivanco International SAC; Perú
Fil: Miguel, M.. Vivanco International SAC; Perú
Fil: Youngs, C. R.. Iowa State University; Estados Unidos
Fil: Vivanco, H. W.. Vivanco International SAC; Perú
Materia
Camelids
Ejaculate
PureSperm®80
Seminal plasma
Viscosity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128953
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/128953
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solutionGómez Quispe, O. E.Gutiérrez Reynoso, G. A.Gallegos Cardenas, A.Fumuso, Fernanda GabrielaAsparrin, M.Asparrin Del Carpio, M.Jara, C. W.Ponce, D.Miguel, M.Youngs, C. R.Vivanco, H. W.CamelidsEjaculatePureSperm®80Seminal plasmaViscosityhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. Aims: The objective of this investigation was to evaluate recovery, motility, and plasma membrane integrity of alpaca spermatozoa after centrifugation in one of two different solutions at one of three different combinations of speed and time. Methods: A total of 24 ejaculates was recovered from seven reproductively sound Huacaya males using a modified artificial vagina (AV) after training the animals for semen collection. A 2 × 3 factorial treatment arrangement was utilized for this study. Ejaculates were divided into fractions for centrifugation in one of two solutions (Tris extender or PureSperm®80 density gradient solution) at one of three combinations of speed and time (492 × g for 15 min, 1968 × g for 10 min, or 4448 × g for 7 min). The experiment was replicated eight times. Results: Analysis revealed that centrifugation at 4448 × g for 7 min in PureSperm®80 provided a high recovery rate of spermatozoa with the highest sperm motility and functional integrity of plasma membrane post-centrifugation. Conclusion: Results suggest that adoption of this procedure (centrifugation at 4448 × g for 7 min in PureSperm®80) in the initial processing of alpaca ejaculates may enhance subsequent ability to use semen for AI and other assisted reproductive biotechnologies in this speciesFil: Gómez Quispe, O. E.. Universidad Nacional Agraria La Molina; Perú. Universidad Nacional Micaela Bastidas de Apurímac; PerúFil: Gutiérrez Reynoso, G. A.. Universidad Nacional Agraria La Molina; PerúFil: Gallegos Cardenas, A.. Universidad Nacional Agraria La Molina; PerúFil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Asparrin, M.. Fundo Mallkini de Michael & Cia S.A.; PerúFil: Asparrin Del Carpio, M.. Fundo Mallkini de Michael & Cia S.A.; PerúFil: Jara, C. W.. Fundo Mallkini de Michael & Cia S.A.; PerúFil: Ponce, D.. Vivanco International SAC; PerúFil: Miguel, M.. Vivanco International SAC; PerúFil: Youngs, C. R.. Iowa State University; Estados UnidosFil: Vivanco, H. W.. Vivanco International SAC; PerúShiraz Univ2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128953Gómez Quispe, O. E.; Gutiérrez Reynoso, G. A.; Gallegos Cardenas, A.; Fumuso, Fernanda Gabriela; Asparrin, M.; et al.; Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution; Shiraz Univ; Iranian Journal Of Veterinary Research; 20; 2; 10-2019; 96-1041728-19972252-0589CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ijvr.shirazu.ac.ir/article_5257.htmlinfo:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/31531031/info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716282/info:eu-repo/semantics/altIdentifier/doi/10.22099/IJVR.2019.5257info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:36Zoai:ri.conicet.gov.ar:11336/128953instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:36.516CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution
title Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution
spellingShingle Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution
Gómez Quispe, O. E.
Camelids
Ejaculate
PureSperm®80
Seminal plasma
Viscosity
title_short Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution
title_full Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution
title_fullStr Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution
title_full_unstemmed Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution
title_sort Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution
dc.creator.none.fl_str_mv Gómez Quispe, O. E.
Gutiérrez Reynoso, G. A.
Gallegos Cardenas, A.
Fumuso, Fernanda Gabriela
Asparrin, M.
Asparrin Del Carpio, M.
Jara, C. W.
Ponce, D.
Miguel, M.
Youngs, C. R.
Vivanco, H. W.
author Gómez Quispe, O. E.
author_facet Gómez Quispe, O. E.
Gutiérrez Reynoso, G. A.
Gallegos Cardenas, A.
Fumuso, Fernanda Gabriela
Asparrin, M.
Asparrin Del Carpio, M.
Jara, C. W.
Ponce, D.
Miguel, M.
Youngs, C. R.
Vivanco, H. W.
author_role author
author2 Gutiérrez Reynoso, G. A.
Gallegos Cardenas, A.
Fumuso, Fernanda Gabriela
Asparrin, M.
Asparrin Del Carpio, M.
Jara, C. W.
Ponce, D.
Miguel, M.
Youngs, C. R.
Vivanco, H. W.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Camelids
Ejaculate
PureSperm®80
Seminal plasma
Viscosity
topic Camelids
Ejaculate
PureSperm®80
Seminal plasma
Viscosity
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. Aims: The objective of this investigation was to evaluate recovery, motility, and plasma membrane integrity of alpaca spermatozoa after centrifugation in one of two different solutions at one of three different combinations of speed and time. Methods: A total of 24 ejaculates was recovered from seven reproductively sound Huacaya males using a modified artificial vagina (AV) after training the animals for semen collection. A 2 × 3 factorial treatment arrangement was utilized for this study. Ejaculates were divided into fractions for centrifugation in one of two solutions (Tris extender or PureSperm®80 density gradient solution) at one of three combinations of speed and time (492 × g for 15 min, 1968 × g for 10 min, or 4448 × g for 7 min). The experiment was replicated eight times. Results: Analysis revealed that centrifugation at 4448 × g for 7 min in PureSperm®80 provided a high recovery rate of spermatozoa with the highest sperm motility and functional integrity of plasma membrane post-centrifugation. Conclusion: Results suggest that adoption of this procedure (centrifugation at 4448 × g for 7 min in PureSperm®80) in the initial processing of alpaca ejaculates may enhance subsequent ability to use semen for AI and other assisted reproductive biotechnologies in this species
Fil: Gómez Quispe, O. E.. Universidad Nacional Agraria La Molina; Perú. Universidad Nacional Micaela Bastidas de Apurímac; Perú
Fil: Gutiérrez Reynoso, G. A.. Universidad Nacional Agraria La Molina; Perú
Fil: Gallegos Cardenas, A.. Universidad Nacional Agraria La Molina; Perú
Fil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asparrin, M.. Fundo Mallkini de Michael & Cia S.A.; Perú
Fil: Asparrin Del Carpio, M.. Fundo Mallkini de Michael & Cia S.A.; Perú
Fil: Jara, C. W.. Fundo Mallkini de Michael & Cia S.A.; Perú
Fil: Ponce, D.. Vivanco International SAC; Perú
Fil: Miguel, M.. Vivanco International SAC; Perú
Fil: Youngs, C. R.. Iowa State University; Estados Unidos
Fil: Vivanco, H. W.. Vivanco International SAC; Perú
description Background: One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. Aims: The objective of this investigation was to evaluate recovery, motility, and plasma membrane integrity of alpaca spermatozoa after centrifugation in one of two different solutions at one of three different combinations of speed and time. Methods: A total of 24 ejaculates was recovered from seven reproductively sound Huacaya males using a modified artificial vagina (AV) after training the animals for semen collection. A 2 × 3 factorial treatment arrangement was utilized for this study. Ejaculates were divided into fractions for centrifugation in one of two solutions (Tris extender or PureSperm®80 density gradient solution) at one of three combinations of speed and time (492 × g for 15 min, 1968 × g for 10 min, or 4448 × g for 7 min). The experiment was replicated eight times. Results: Analysis revealed that centrifugation at 4448 × g for 7 min in PureSperm®80 provided a high recovery rate of spermatozoa with the highest sperm motility and functional integrity of plasma membrane post-centrifugation. Conclusion: Results suggest that adoption of this procedure (centrifugation at 4448 × g for 7 min in PureSperm®80) in the initial processing of alpaca ejaculates may enhance subsequent ability to use semen for AI and other assisted reproductive biotechnologies in this species
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128953
Gómez Quispe, O. E.; Gutiérrez Reynoso, G. A.; Gallegos Cardenas, A.; Fumuso, Fernanda Gabriela; Asparrin, M.; et al.; Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution; Shiraz Univ; Iranian Journal Of Veterinary Research; 20; 2; 10-2019; 96-104
1728-1997
2252-0589
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128953
identifier_str_mv Gómez Quispe, O. E.; Gutiérrez Reynoso, G. A.; Gallegos Cardenas, A.; Fumuso, Fernanda Gabriela; Asparrin, M.; et al.; Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution; Shiraz Univ; Iranian Journal Of Veterinary Research; 20; 2; 10-2019; 96-104
1728-1997
2252-0589
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ijvr.shirazu.ac.ir/article_5257.html
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/31531031/
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716282/
info:eu-repo/semantics/altIdentifier/doi/10.22099/IJVR.2019.5257
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Shiraz Univ
publisher.none.fl_str_mv Shiraz Univ
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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