Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses

Autores
Fiorentino, Maria Andrea; Paolicchi, Fernando Alberto; Campero, Carlos Manuel; Barbeito, Claudio Gustavo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the identification of complex structures to which the terminal sugar attaches and may reveal physiological or pathological changes in cells, intracellular interactions or extracellular transport pathways. This study was carried out to investigate the effect of infection with Brucella abortus on the pattern of lectin binding in bovine fetal lungs (n=6) and bovine placentas (n=5). Fetal lungs and placenta from heifers experimentally inoculated with B. abortus, strain 2308 were examined by histological, lectin-histochemical, immunohistochemical and cultural techniques. B. abortus antigens were immunohistochemically detected in fetal lungs and placenta. An increase in the labeling with UEA-1, DBA, PNA, RCA-1 and SBA was found in the lungs and an increase in the labeling with UEA-1, ConA, PNA, DBA was found in the placentas. The present lectin histochemical study revealed a distinctive pattern of oligosaccharide distribution in the lungs and placenta of B. abortus-infected fetuses.
Fil: Fiorentino, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Paolicchi, Fernando Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
BOVINE FETUSES
BRUCELLA ABORTUS
IMMUNOHISTOCHEMICAL
LECTIN
PLACENTA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/101378
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetusesFiorentino, Maria AndreaPaolicchi, Fernando AlbertoCampero, Carlos ManuelBarbeito, Claudio GustavoBOVINE FETUSESBRUCELLA ABORTUSIMMUNOHISTOCHEMICALLECTINPLACENTAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the identification of complex structures to which the terminal sugar attaches and may reveal physiological or pathological changes in cells, intracellular interactions or extracellular transport pathways. This study was carried out to investigate the effect of infection with Brucella abortus on the pattern of lectin binding in bovine fetal lungs (n=6) and bovine placentas (n=5). Fetal lungs and placenta from heifers experimentally inoculated with B. abortus, strain 2308 were examined by histological, lectin-histochemical, immunohistochemical and cultural techniques. B. abortus antigens were immunohistochemically detected in fetal lungs and placenta. An increase in the labeling with UEA-1, DBA, PNA, RCA-1 and SBA was found in the lungs and an increase in the labeling with UEA-1, ConA, PNA, DBA was found in the placentas. The present lectin histochemical study revealed a distinctive pattern of oligosaccharide distribution in the lungs and placenta of B. abortus-infected fetuses.Fil: Fiorentino, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Paolicchi, Fernando Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaUniversity of Tripoli. Faculty of Veterinary Medicine2018-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101378Fiorentino, Maria Andrea; Paolicchi, Fernando Alberto; Campero, Carlos Manuel; Barbeito, Claudio Gustavo; Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 8; 1; 2-2018; 57-632226-44852218-6050CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ajol.info/index.php/ovj/article/view/167390info:eu-repo/semantics/altIdentifier/doi/10.4314/ovj.v8i1.10info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:08Zoai:ri.conicet.gov.ar:11336/101378instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:08.655CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses
title Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses
spellingShingle Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses
Fiorentino, Maria Andrea
BOVINE FETUSES
BRUCELLA ABORTUS
IMMUNOHISTOCHEMICAL
LECTIN
PLACENTA
title_short Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses
title_full Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses
title_fullStr Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses
title_full_unstemmed Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses
title_sort Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses
dc.creator.none.fl_str_mv Fiorentino, Maria Andrea
Paolicchi, Fernando Alberto
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
author Fiorentino, Maria Andrea
author_facet Fiorentino, Maria Andrea
Paolicchi, Fernando Alberto
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
author_role author
author2 Paolicchi, Fernando Alberto
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
author2_role author
author
author
dc.subject.none.fl_str_mv BOVINE FETUSES
BRUCELLA ABORTUS
IMMUNOHISTOCHEMICAL
LECTIN
PLACENTA
topic BOVINE FETUSES
BRUCELLA ABORTUS
IMMUNOHISTOCHEMICAL
LECTIN
PLACENTA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the identification of complex structures to which the terminal sugar attaches and may reveal physiological or pathological changes in cells, intracellular interactions or extracellular transport pathways. This study was carried out to investigate the effect of infection with Brucella abortus on the pattern of lectin binding in bovine fetal lungs (n=6) and bovine placentas (n=5). Fetal lungs and placenta from heifers experimentally inoculated with B. abortus, strain 2308 were examined by histological, lectin-histochemical, immunohistochemical and cultural techniques. B. abortus antigens were immunohistochemically detected in fetal lungs and placenta. An increase in the labeling with UEA-1, DBA, PNA, RCA-1 and SBA was found in the lungs and an increase in the labeling with UEA-1, ConA, PNA, DBA was found in the placentas. The present lectin histochemical study revealed a distinctive pattern of oligosaccharide distribution in the lungs and placenta of B. abortus-infected fetuses.
Fil: Fiorentino, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Paolicchi, Fernando Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the identification of complex structures to which the terminal sugar attaches and may reveal physiological or pathological changes in cells, intracellular interactions or extracellular transport pathways. This study was carried out to investigate the effect of infection with Brucella abortus on the pattern of lectin binding in bovine fetal lungs (n=6) and bovine placentas (n=5). Fetal lungs and placenta from heifers experimentally inoculated with B. abortus, strain 2308 were examined by histological, lectin-histochemical, immunohistochemical and cultural techniques. B. abortus antigens were immunohistochemically detected in fetal lungs and placenta. An increase in the labeling with UEA-1, DBA, PNA, RCA-1 and SBA was found in the lungs and an increase in the labeling with UEA-1, ConA, PNA, DBA was found in the placentas. The present lectin histochemical study revealed a distinctive pattern of oligosaccharide distribution in the lungs and placenta of B. abortus-infected fetuses.
publishDate 2018
dc.date.none.fl_str_mv 2018-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/101378
Fiorentino, Maria Andrea; Paolicchi, Fernando Alberto; Campero, Carlos Manuel; Barbeito, Claudio Gustavo; Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 8; 1; 2-2018; 57-63
2226-4485
2218-6050
CONICET Digital
CONICET
url http://hdl.handle.net/11336/101378
identifier_str_mv Fiorentino, Maria Andrea; Paolicchi, Fernando Alberto; Campero, Carlos Manuel; Barbeito, Claudio Gustavo; Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 8; 1; 2-2018; 57-63
2226-4485
2218-6050
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ajol.info/index.php/ovj/article/view/167390
info:eu-repo/semantics/altIdentifier/doi/10.4314/ovj.v8i1.10
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv University of Tripoli. Faculty of Veterinary Medicine
publisher.none.fl_str_mv University of Tripoli. Faculty of Veterinary Medicine
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269783887708160
score 13.13397

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