Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage

Autores
Hernández, Martín; Gonzalo, Tomas; Marandino, Ana; Iraola, Gregorio; Maya, Leticia; Mattion, Nora Marta; Hernandez, Diego; Villegas, Pedro; Banda, Alejandro; Panzera, Yanina; Perez, Ruben
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Infectious Bursal Disease Virus (IBDV) is one of the most concerning sanitary problems to the world poultry production. IBDV comprises four well-defined evolutionary lineages known as classic (cIBDV), classic virulent (cvIBDV), variant (vaIBDV) and hypervirulent (vvIBDV) strains. In the present study, we characterized IBDV samples in Argentina and Uruguay by sequencing the coding region of the hypervariable domain of the capsid protein VP2. Samples belonging to three strains (cIBDV, vvIBDV, and cvIBDV) were unambiguously classified by the presence of molecular markers and phylogenetic analysis. Notably, a high proportion of samples (60 %) could not be accurately assign to any of the previously described strains, and were then denoted as novel IBDV (nIBDV). These Uruguayan and Argentine nIBDVs constitute an independent evolutionary lineage that also includes viruses from others countries of America and Asia. The hypervariable VP2 sequence of these nIBDVs shares amino acids markers with other IBDV strains, but has a unique and conserved molecular signature (272T, 289P, 290I and 296F) that may be considered a diagnostic character for classification. A discriminant analysis of principal components (DAPC) also identified the nIBDVs as a cluster of genetically related viruses separated from the typical IBDV strains. DAPC and genetic distance estimation indicated that the nIBDV is one of the most genetically divergent lineages of IBDV. Together, the present study suggests that the highly divergent nIBDV lineage is a previously undescribed IBDV group that is widely circulating in the world poultry production. Further studies using antigenic, pathogenic, epidemiologic, and additional genetic studies are needed to completely characterize this lineage and determine if it should be considered alongside conventional IBDV strains.
Fil: Hernández, Martín. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Gonzalo, Tomas. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Marandino, Ana. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Iraola, Gregorio. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Maya, Leticia. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología ; Argentina
Fil: Hernandez, Diego. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Villegas, Pedro. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Banda, Alejandro. Mississippi State University. College of Veterinary Medicine. Poultry Research and Diagnostic Laboratory; Estados Unidos
Fil: Panzera, Yanina. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Perez, Ruben. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Materia
Infectious Bursal Disease Virus
Evolution
Lineage
Strains
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4152
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineageHernández, MartínGonzalo, TomasMarandino, AnaIraola, GregorioMaya, LeticiaMattion, Nora MartaHernandez, DiegoVillegas, PedroBanda, AlejandroPanzera, YaninaPerez, RubenInfectious Bursal Disease VirusEvolutionLineageStrainshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Infectious Bursal Disease Virus (IBDV) is one of the most concerning sanitary problems to the world poultry production. IBDV comprises four well-defined evolutionary lineages known as classic (cIBDV), classic virulent (cvIBDV), variant (vaIBDV) and hypervirulent (vvIBDV) strains. In the present study, we characterized IBDV samples in Argentina and Uruguay by sequencing the coding region of the hypervariable domain of the capsid protein VP2. Samples belonging to three strains (cIBDV, vvIBDV, and cvIBDV) were unambiguously classified by the presence of molecular markers and phylogenetic analysis. Notably, a high proportion of samples (60 %) could not be accurately assign to any of the previously described strains, and were then denoted as novel IBDV (nIBDV). These Uruguayan and Argentine nIBDVs constitute an independent evolutionary lineage that also includes viruses from others countries of America and Asia. The hypervariable VP2 sequence of these nIBDVs shares amino acids markers with other IBDV strains, but has a unique and conserved molecular signature (272T, 289P, 290I and 296F) that may be considered a diagnostic character for classification. A discriminant analysis of principal components (DAPC) also identified the nIBDVs as a cluster of genetically related viruses separated from the typical IBDV strains. DAPC and genetic distance estimation indicated that the nIBDV is one of the most genetically divergent lineages of IBDV. Together, the present study suggests that the highly divergent nIBDV lineage is a previously undescribed IBDV group that is widely circulating in the world poultry production. Further studies using antigenic, pathogenic, epidemiologic, and additional genetic studies are needed to completely characterize this lineage and determine if it should be considered alongside conventional IBDV strains.Fil: Hernández, Martín. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; UruguayFil: Gonzalo, Tomas. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; UruguayFil: Marandino, Ana. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; UruguayFil: Iraola, Gregorio. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; UruguayFil: Maya, Leticia. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; UruguayFil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología ; ArgentinaFil: Hernandez, Diego. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; UruguayFil: Villegas, Pedro. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados UnidosFil: Banda, Alejandro. Mississippi State University. College of Veterinary Medicine. Poultry Research and Diagnostic Laboratory; Estados UnidosFil: Panzera, Yanina. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; UruguayFil: Perez, Ruben. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; UruguayTaylor & Francis2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4152Hernández, Martín; Gonzalo, Tomas; Marandino, Ana; Iraola, Gregorio; Maya, Leticia; et al.; Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage; Taylor & Francis; Avian Pathology; 44; 3; 3-2015; 212-2210307-9457enginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/03079457.2015.1025696?journalCode=cavp20info:eu-repo/semantics/altIdentifier/doi/10.1080/03079457.2015.1025696info:eu-repo/semantics/altIdentifier/issn/0307-9457info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:21Zoai:ri.conicet.gov.ar:11336/4152instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:22.193CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage
title Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage
spellingShingle Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage
Hernández, Martín
Infectious Bursal Disease Virus
Evolution
Lineage
Strains
title_short Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage
title_full Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage
title_fullStr Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage
title_full_unstemmed Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage
title_sort Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage
dc.creator.none.fl_str_mv Hernández, Martín
Gonzalo, Tomas
Marandino, Ana
Iraola, Gregorio
Maya, Leticia
Mattion, Nora Marta
Hernandez, Diego
Villegas, Pedro
Banda, Alejandro
Panzera, Yanina
Perez, Ruben
author Hernández, Martín
author_facet Hernández, Martín
Gonzalo, Tomas
Marandino, Ana
Iraola, Gregorio
Maya, Leticia
Mattion, Nora Marta
Hernandez, Diego
Villegas, Pedro
Banda, Alejandro
Panzera, Yanina
Perez, Ruben
author_role author
author2 Gonzalo, Tomas
Marandino, Ana
Iraola, Gregorio
Maya, Leticia
Mattion, Nora Marta
Hernandez, Diego
Villegas, Pedro
Banda, Alejandro
Panzera, Yanina
Perez, Ruben
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Infectious Bursal Disease Virus
Evolution
Lineage
Strains
topic Infectious Bursal Disease Virus
Evolution
Lineage
Strains
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Infectious Bursal Disease Virus (IBDV) is one of the most concerning sanitary problems to the world poultry production. IBDV comprises four well-defined evolutionary lineages known as classic (cIBDV), classic virulent (cvIBDV), variant (vaIBDV) and hypervirulent (vvIBDV) strains. In the present study, we characterized IBDV samples in Argentina and Uruguay by sequencing the coding region of the hypervariable domain of the capsid protein VP2. Samples belonging to three strains (cIBDV, vvIBDV, and cvIBDV) were unambiguously classified by the presence of molecular markers and phylogenetic analysis. Notably, a high proportion of samples (60 %) could not be accurately assign to any of the previously described strains, and were then denoted as novel IBDV (nIBDV). These Uruguayan and Argentine nIBDVs constitute an independent evolutionary lineage that also includes viruses from others countries of America and Asia. The hypervariable VP2 sequence of these nIBDVs shares amino acids markers with other IBDV strains, but has a unique and conserved molecular signature (272T, 289P, 290I and 296F) that may be considered a diagnostic character for classification. A discriminant analysis of principal components (DAPC) also identified the nIBDVs as a cluster of genetically related viruses separated from the typical IBDV strains. DAPC and genetic distance estimation indicated that the nIBDV is one of the most genetically divergent lineages of IBDV. Together, the present study suggests that the highly divergent nIBDV lineage is a previously undescribed IBDV group that is widely circulating in the world poultry production. Further studies using antigenic, pathogenic, epidemiologic, and additional genetic studies are needed to completely characterize this lineage and determine if it should be considered alongside conventional IBDV strains.
Fil: Hernández, Martín. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Gonzalo, Tomas. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Marandino, Ana. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Iraola, Gregorio. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Maya, Leticia. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología ; Argentina
Fil: Hernandez, Diego. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Villegas, Pedro. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Banda, Alejandro. Mississippi State University. College of Veterinary Medicine. Poultry Research and Diagnostic Laboratory; Estados Unidos
Fil: Panzera, Yanina. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
Fil: Perez, Ruben. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva, Departamento de Biología Animal; Uruguay
description Infectious Bursal Disease Virus (IBDV) is one of the most concerning sanitary problems to the world poultry production. IBDV comprises four well-defined evolutionary lineages known as classic (cIBDV), classic virulent (cvIBDV), variant (vaIBDV) and hypervirulent (vvIBDV) strains. In the present study, we characterized IBDV samples in Argentina and Uruguay by sequencing the coding region of the hypervariable domain of the capsid protein VP2. Samples belonging to three strains (cIBDV, vvIBDV, and cvIBDV) were unambiguously classified by the presence of molecular markers and phylogenetic analysis. Notably, a high proportion of samples (60 %) could not be accurately assign to any of the previously described strains, and were then denoted as novel IBDV (nIBDV). These Uruguayan and Argentine nIBDVs constitute an independent evolutionary lineage that also includes viruses from others countries of America and Asia. The hypervariable VP2 sequence of these nIBDVs shares amino acids markers with other IBDV strains, but has a unique and conserved molecular signature (272T, 289P, 290I and 296F) that may be considered a diagnostic character for classification. A discriminant analysis of principal components (DAPC) also identified the nIBDVs as a cluster of genetically related viruses separated from the typical IBDV strains. DAPC and genetic distance estimation indicated that the nIBDV is one of the most genetically divergent lineages of IBDV. Together, the present study suggests that the highly divergent nIBDV lineage is a previously undescribed IBDV group that is widely circulating in the world poultry production. Further studies using antigenic, pathogenic, epidemiologic, and additional genetic studies are needed to completely characterize this lineage and determine if it should be considered alongside conventional IBDV strains.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4152
Hernández, Martín; Gonzalo, Tomas; Marandino, Ana; Iraola, Gregorio; Maya, Leticia; et al.; Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage; Taylor & Francis; Avian Pathology; 44; 3; 3-2015; 212-221
0307-9457
url http://hdl.handle.net/11336/4152
identifier_str_mv Hernández, Martín; Gonzalo, Tomas; Marandino, Ana; Iraola, Gregorio; Maya, Leticia; et al.; Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage; Taylor & Francis; Avian Pathology; 44; 3; 3-2015; 212-221
0307-9457
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/03079457.2015.1025696?journalCode=cavp20
info:eu-repo/semantics/altIdentifier/doi/10.1080/03079457.2015.1025696
info:eu-repo/semantics/altIdentifier/issn/0307-9457
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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