Improving reproducibility between batches of silver nanoparticles using an experimental design approach

Autores
Núñez, Rodrigo Nicolás; Veglia, Alicia Viviana; Pacioni, Natalia Lorena
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An optimized method for the synthesis of silver nanoparticles (AgNP) using gallic acid as reductant was achieved using design of experiment strategies based on response surface methodologies. Fractional Factorial Design was used in the screening stage, the Box-Behnken method was employed to model the target responses and finally, the optimization step was done using Desirability function. The obtained AgNP presented improved repetitivity and reproducibility of photophysical properties between batches compared to the synthesis method reported in literature. Intra-assays, intermediate precision and reproducibility tests were performed and proved the different AgNP batches presented equal optical responses, average size and size distribution at a 95% confidence level. In addition, a test on shelf life estimated the optimized AgNP preserve their properties at least for 38 days and especially, zeta potential measurements indicated their low tendency to flocculation as long as 120 days. Furthermore, a remarkable improvement in obtaining reproducible Stern-Volmer constants in fluorescence quenching experiments using Carbazole as fluorophore was observed compared to nanoparticles synthesized by a non-optimal method.
Fil: Núñez, Rodrigo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Pacioni, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
BOX-BEHNKEN
FLUORESCENCE QUENCHING
FRACTIONAL FACTORIAL DESIGN
GALLIC ACID
RESPONSE SURFACE MODELS
SILVER NANOPARTICLES SYNTHESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95523
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/95523
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Improving reproducibility between batches of silver nanoparticles using an experimental design approachNúñez, Rodrigo NicolásVeglia, Alicia VivianaPacioni, Natalia LorenaBOX-BEHNKENFLUORESCENCE QUENCHINGFRACTIONAL FACTORIAL DESIGNGALLIC ACIDRESPONSE SURFACE MODELSSILVER NANOPARTICLES SYNTHESIShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An optimized method for the synthesis of silver nanoparticles (AgNP) using gallic acid as reductant was achieved using design of experiment strategies based on response surface methodologies. Fractional Factorial Design was used in the screening stage, the Box-Behnken method was employed to model the target responses and finally, the optimization step was done using Desirability function. The obtained AgNP presented improved repetitivity and reproducibility of photophysical properties between batches compared to the synthesis method reported in literature. Intra-assays, intermediate precision and reproducibility tests were performed and proved the different AgNP batches presented equal optical responses, average size and size distribution at a 95% confidence level. In addition, a test on shelf life estimated the optimized AgNP preserve their properties at least for 38 days and especially, zeta potential measurements indicated their low tendency to flocculation as long as 120 days. Furthermore, a remarkable improvement in obtaining reproducible Stern-Volmer constants in fluorescence quenching experiments using Carbazole as fluorophore was observed compared to nanoparticles synthesized by a non-optimal method.Fil: Núñez, Rodrigo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Pacioni, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95523Núñez, Rodrigo Nicolás; Veglia, Alicia Viviana; Pacioni, Natalia Lorena; Improving reproducibility between batches of silver nanoparticles using an experimental design approach; Elsevier Science; Microchemical Journal; 141; 9-2018; 110-1170026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X18303151info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2018.05.017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:14Zoai:ri.conicet.gov.ar:11336/95523instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:14.369CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Improving reproducibility between batches of silver nanoparticles using an experimental design approach
title Improving reproducibility between batches of silver nanoparticles using an experimental design approach
spellingShingle Improving reproducibility between batches of silver nanoparticles using an experimental design approach
Núñez, Rodrigo Nicolás
BOX-BEHNKEN
FLUORESCENCE QUENCHING
FRACTIONAL FACTORIAL DESIGN
GALLIC ACID
RESPONSE SURFACE MODELS
SILVER NANOPARTICLES SYNTHESIS
title_short Improving reproducibility between batches of silver nanoparticles using an experimental design approach
title_full Improving reproducibility between batches of silver nanoparticles using an experimental design approach
title_fullStr Improving reproducibility between batches of silver nanoparticles using an experimental design approach
title_full_unstemmed Improving reproducibility between batches of silver nanoparticles using an experimental design approach
title_sort Improving reproducibility between batches of silver nanoparticles using an experimental design approach
dc.creator.none.fl_str_mv Núñez, Rodrigo Nicolás
Veglia, Alicia Viviana
Pacioni, Natalia Lorena
author Núñez, Rodrigo Nicolás
author_facet Núñez, Rodrigo Nicolás
Veglia, Alicia Viviana
Pacioni, Natalia Lorena
author_role author
author2 Veglia, Alicia Viviana
Pacioni, Natalia Lorena
author2_role author
author
dc.subject.none.fl_str_mv BOX-BEHNKEN
FLUORESCENCE QUENCHING
FRACTIONAL FACTORIAL DESIGN
GALLIC ACID
RESPONSE SURFACE MODELS
SILVER NANOPARTICLES SYNTHESIS
topic BOX-BEHNKEN
FLUORESCENCE QUENCHING
FRACTIONAL FACTORIAL DESIGN
GALLIC ACID
RESPONSE SURFACE MODELS
SILVER NANOPARTICLES SYNTHESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An optimized method for the synthesis of silver nanoparticles (AgNP) using gallic acid as reductant was achieved using design of experiment strategies based on response surface methodologies. Fractional Factorial Design was used in the screening stage, the Box-Behnken method was employed to model the target responses and finally, the optimization step was done using Desirability function. The obtained AgNP presented improved repetitivity and reproducibility of photophysical properties between batches compared to the synthesis method reported in literature. Intra-assays, intermediate precision and reproducibility tests were performed and proved the different AgNP batches presented equal optical responses, average size and size distribution at a 95% confidence level. In addition, a test on shelf life estimated the optimized AgNP preserve their properties at least for 38 days and especially, zeta potential measurements indicated their low tendency to flocculation as long as 120 days. Furthermore, a remarkable improvement in obtaining reproducible Stern-Volmer constants in fluorescence quenching experiments using Carbazole as fluorophore was observed compared to nanoparticles synthesized by a non-optimal method.
Fil: Núñez, Rodrigo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Pacioni, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description An optimized method for the synthesis of silver nanoparticles (AgNP) using gallic acid as reductant was achieved using design of experiment strategies based on response surface methodologies. Fractional Factorial Design was used in the screening stage, the Box-Behnken method was employed to model the target responses and finally, the optimization step was done using Desirability function. The obtained AgNP presented improved repetitivity and reproducibility of photophysical properties between batches compared to the synthesis method reported in literature. Intra-assays, intermediate precision and reproducibility tests were performed and proved the different AgNP batches presented equal optical responses, average size and size distribution at a 95% confidence level. In addition, a test on shelf life estimated the optimized AgNP preserve their properties at least for 38 days and especially, zeta potential measurements indicated their low tendency to flocculation as long as 120 days. Furthermore, a remarkable improvement in obtaining reproducible Stern-Volmer constants in fluorescence quenching experiments using Carbazole as fluorophore was observed compared to nanoparticles synthesized by a non-optimal method.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95523
Núñez, Rodrigo Nicolás; Veglia, Alicia Viviana; Pacioni, Natalia Lorena; Improving reproducibility between batches of silver nanoparticles using an experimental design approach; Elsevier Science; Microchemical Journal; 141; 9-2018; 110-117
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95523
identifier_str_mv Núñez, Rodrigo Nicolás; Veglia, Alicia Viviana; Pacioni, Natalia Lorena; Improving reproducibility between batches of silver nanoparticles using an experimental design approach; Elsevier Science; Microchemical Journal; 141; 9-2018; 110-117
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X18303151
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2018.05.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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