B-cell lymphopoiesis is influenced by cathepsin L

Autores
Badano, Maria Noel; Camicia, Gabriela Lorena; Lombardi, Maria Gabriela; Maglioco, Andrea Florencia; Cabrera, Gabriel Gustavo; Costa, Hector Luis; Meiss, Roberto Pablo; Piazzon, Margarita Isabel; Nepomnaschy, Irene
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cathepsin L (CTSL) is a ubiquitously expressed lysosomal cysteine peptidase with diverse and highly specific functions. The involvement of CTSL in thymic CD4+ T-cell positive selection has been well documented. Using CTSLnkt/nkt mice that lack CTSL activity, we have previously demonstrated that the absence of CTSL activity affects the homeostasis of the T-cell pool by decreasing CD4+ cell thymic production and increasing CD8+ thymocyte production. Herein we investigated the influence of CTSL activity on the homeostasis of peripheral B-cell populations and bone marrow (BM) B-cell maturation. B-cell numbers were increased in lymph nodes (LN), spleen and blood from CTSLnkt/nkt mice. Increases in splenic B-cell numbers were restricted to transitional T1 and T2 cells and to the marginal zone (MZ) cell subpopulation. No alterations in the proliferative or apoptosis levels were detected in peripheral B-cell populations from CTSLnkt/nkt mice. In the BM, the percentage and the absolute number of pre-pro-B, pro-B, pre-B, immature and mature B cells were not altered. However, and experiments showed that BM B-cell production was markedly increased in CTSLnkt/nkt mice. Besides, BM B-cell emigration to the spleen was increased in CTSLnkt/nkt mice. Colony-forming unit pre-B (CFU pre-B) assays in the presence of BM stromal cells (SC) and reciprocal BM chimeras revealed that both BM B-cell precursors and SC would contribute to sustain the increased B-cell hematopoiesis in CTSLnkt/nkt mice. Overall, our data clearly demonstrate that CTSL negatively regulates BM B-cell production and output therefore influencing the homeostasis of peripheral B cells.
Fil: Badano, Maria Noel. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Fil: Camicia, Gabriela Lorena. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Fil: Lombardi, Maria Gabriela. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Estudios Farmacologicos y Botánicos; Argentina;
Fil: Maglioco, Andrea Florencia. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Fil: Cabrera, Gabriel Gustavo. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Fil: Costa, Hector Luis. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Fil: Meiss, Roberto Pablo. Academia Nacional de Medicina de Buenos Aires; Argentina;
Fil: Piazzon, Margarita Isabel. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Fil: Nepomnaschy, Irene. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Materia
Cathepsin L
B cells
lymphopoiesis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1314
Acceder
id CONICETDig_dec5e1e1afdccd440e5e4fb9ea7cb64b
oai_identifier_str oai:ri.conicet.gov.ar:11336/1314
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling B-cell lymphopoiesis is influenced by cathepsin LBadano, Maria NoelCamicia, Gabriela LorenaLombardi, Maria GabrielaMaglioco, Andrea FlorenciaCabrera, Gabriel GustavoCosta, Hector LuisMeiss, Roberto PabloPiazzon, Margarita IsabelNepomnaschy, IreneCathepsin LB cellslymphopoiesishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Cathepsin L (CTSL) is a ubiquitously expressed lysosomal cysteine peptidase with diverse and highly specific functions. The involvement of CTSL in thymic CD4+ T-cell positive selection has been well documented. Using CTSLnkt/nkt mice that lack CTSL activity, we have previously demonstrated that the absence of CTSL activity affects the homeostasis of the T-cell pool by decreasing CD4+ cell thymic production and increasing CD8+ thymocyte production. Herein we investigated the influence of CTSL activity on the homeostasis of peripheral B-cell populations and bone marrow (BM) B-cell maturation. B-cell numbers were increased in lymph nodes (LN), spleen and blood from CTSLnkt/nkt mice. Increases in splenic B-cell numbers were restricted to transitional T1 and T2 cells and to the marginal zone (MZ) cell subpopulation. No alterations in the proliferative or apoptosis levels were detected in peripheral B-cell populations from CTSLnkt/nkt mice. In the BM, the percentage and the absolute number of pre-pro-B, pro-B, pre-B, immature and mature B cells were not altered. However, and experiments showed that BM B-cell production was markedly increased in CTSLnkt/nkt mice. Besides, BM B-cell emigration to the spleen was increased in CTSLnkt/nkt mice. Colony-forming unit pre-B (CFU pre-B) assays in the presence of BM stromal cells (SC) and reciprocal BM chimeras revealed that both BM B-cell precursors and SC would contribute to sustain the increased B-cell hematopoiesis in CTSLnkt/nkt mice. Overall, our data clearly demonstrate that CTSL negatively regulates BM B-cell production and output therefore influencing the homeostasis of peripheral B cells.Fil: Badano, Maria Noel. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;Fil: Camicia, Gabriela Lorena. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;Fil: Lombardi, Maria Gabriela. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Estudios Farmacologicos y Botánicos; Argentina;Fil: Maglioco, Andrea Florencia. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;Fil: Cabrera, Gabriel Gustavo. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;Fil: Costa, Hector Luis. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;Fil: Meiss, Roberto Pablo. Academia Nacional de Medicina de Buenos Aires; Argentina;Fil: Piazzon, Margarita Isabel. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;Fil: Nepomnaschy, Irene. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;Public Library Science2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1314Badano, Maria Noel; Camicia, Gabriela Lorena; Lombardi, Maria Gabriela; Maglioco, Andrea Florencia; Cabrera, Gabriel Gustavo; et al.; B-cell lymphopoiesis is influenced by cathepsin L; Public Library Science; Plos One; 8; 4; 4-2013; e613471932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.10.1371/journal.pone.0061347info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:48Zoai:ri.conicet.gov.ar:11336/1314instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:48.585CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv B-cell lymphopoiesis is influenced by cathepsin L
title B-cell lymphopoiesis is influenced by cathepsin L
spellingShingle B-cell lymphopoiesis is influenced by cathepsin L
Badano, Maria Noel
Cathepsin L
B cells
lymphopoiesis
title_short B-cell lymphopoiesis is influenced by cathepsin L
title_full B-cell lymphopoiesis is influenced by cathepsin L
title_fullStr B-cell lymphopoiesis is influenced by cathepsin L
title_full_unstemmed B-cell lymphopoiesis is influenced by cathepsin L
title_sort B-cell lymphopoiesis is influenced by cathepsin L
dc.creator.none.fl_str_mv Badano, Maria Noel
Camicia, Gabriela Lorena
Lombardi, Maria Gabriela
Maglioco, Andrea Florencia
Cabrera, Gabriel Gustavo
Costa, Hector Luis
Meiss, Roberto Pablo
Piazzon, Margarita Isabel
Nepomnaschy, Irene
author Badano, Maria Noel
author_facet Badano, Maria Noel
Camicia, Gabriela Lorena
Lombardi, Maria Gabriela
Maglioco, Andrea Florencia
Cabrera, Gabriel Gustavo
Costa, Hector Luis
Meiss, Roberto Pablo
Piazzon, Margarita Isabel
Nepomnaschy, Irene
author_role author
author2 Camicia, Gabriela Lorena
Lombardi, Maria Gabriela
Maglioco, Andrea Florencia
Cabrera, Gabriel Gustavo
Costa, Hector Luis
Meiss, Roberto Pablo
Piazzon, Margarita Isabel
Nepomnaschy, Irene
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cathepsin L
B cells
lymphopoiesis
topic Cathepsin L
B cells
lymphopoiesis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Cathepsin L (CTSL) is a ubiquitously expressed lysosomal cysteine peptidase with diverse and highly specific functions. The involvement of CTSL in thymic CD4+ T-cell positive selection has been well documented. Using CTSLnkt/nkt mice that lack CTSL activity, we have previously demonstrated that the absence of CTSL activity affects the homeostasis of the T-cell pool by decreasing CD4+ cell thymic production and increasing CD8+ thymocyte production. Herein we investigated the influence of CTSL activity on the homeostasis of peripheral B-cell populations and bone marrow (BM) B-cell maturation. B-cell numbers were increased in lymph nodes (LN), spleen and blood from CTSLnkt/nkt mice. Increases in splenic B-cell numbers were restricted to transitional T1 and T2 cells and to the marginal zone (MZ) cell subpopulation. No alterations in the proliferative or apoptosis levels were detected in peripheral B-cell populations from CTSLnkt/nkt mice. In the BM, the percentage and the absolute number of pre-pro-B, pro-B, pre-B, immature and mature B cells were not altered. However, and experiments showed that BM B-cell production was markedly increased in CTSLnkt/nkt mice. Besides, BM B-cell emigration to the spleen was increased in CTSLnkt/nkt mice. Colony-forming unit pre-B (CFU pre-B) assays in the presence of BM stromal cells (SC) and reciprocal BM chimeras revealed that both BM B-cell precursors and SC would contribute to sustain the increased B-cell hematopoiesis in CTSLnkt/nkt mice. Overall, our data clearly demonstrate that CTSL negatively regulates BM B-cell production and output therefore influencing the homeostasis of peripheral B cells.
Fil: Badano, Maria Noel. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Fil: Camicia, Gabriela Lorena. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Fil: Lombardi, Maria Gabriela. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Centro de Estudios Farmacologicos y Botánicos; Argentina;
Fil: Maglioco, Andrea Florencia. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Fil: Cabrera, Gabriel Gustavo. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Fil: Costa, Hector Luis. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Fil: Meiss, Roberto Pablo. Academia Nacional de Medicina de Buenos Aires; Argentina;
Fil: Piazzon, Margarita Isabel. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
Fil: Nepomnaschy, Irene. Consejo Nacional de Invest.cientif.y Tecnicas. Instituto de Medicina Experimental; Argentina;
description Cathepsin L (CTSL) is a ubiquitously expressed lysosomal cysteine peptidase with diverse and highly specific functions. The involvement of CTSL in thymic CD4+ T-cell positive selection has been well documented. Using CTSLnkt/nkt mice that lack CTSL activity, we have previously demonstrated that the absence of CTSL activity affects the homeostasis of the T-cell pool by decreasing CD4+ cell thymic production and increasing CD8+ thymocyte production. Herein we investigated the influence of CTSL activity on the homeostasis of peripheral B-cell populations and bone marrow (BM) B-cell maturation. B-cell numbers were increased in lymph nodes (LN), spleen and blood from CTSLnkt/nkt mice. Increases in splenic B-cell numbers were restricted to transitional T1 and T2 cells and to the marginal zone (MZ) cell subpopulation. No alterations in the proliferative or apoptosis levels were detected in peripheral B-cell populations from CTSLnkt/nkt mice. In the BM, the percentage and the absolute number of pre-pro-B, pro-B, pre-B, immature and mature B cells were not altered. However, and experiments showed that BM B-cell production was markedly increased in CTSLnkt/nkt mice. Besides, BM B-cell emigration to the spleen was increased in CTSLnkt/nkt mice. Colony-forming unit pre-B (CFU pre-B) assays in the presence of BM stromal cells (SC) and reciprocal BM chimeras revealed that both BM B-cell precursors and SC would contribute to sustain the increased B-cell hematopoiesis in CTSLnkt/nkt mice. Overall, our data clearly demonstrate that CTSL negatively regulates BM B-cell production and output therefore influencing the homeostasis of peripheral B cells.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1314
Badano, Maria Noel; Camicia, Gabriela Lorena; Lombardi, Maria Gabriela; Maglioco, Andrea Florencia; Cabrera, Gabriel Gustavo; et al.; B-cell lymphopoiesis is influenced by cathepsin L; Public Library Science; Plos One; 8; 4; 4-2013; e61347
1932-6203
url http://hdl.handle.net/11336/1314
identifier_str_mv Badano, Maria Noel; Camicia, Gabriela Lorena; Lombardi, Maria Gabriela; Maglioco, Andrea Florencia; Cabrera, Gabriel Gustavo; et al.; B-cell lymphopoiesis is influenced by cathepsin L; Public Library Science; Plos One; 8; 4; 4-2013; e61347
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dx.doi.10.1371/journal.pone.0061347
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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