Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's disease

Autores
Leiva, Natalia Lorena; Bonaccorso Marinelli, María Paula; Carvelli, Flavia Lorena; Sosa Escudero, Miguel Angel; Cabrera Kreiker, Ricardo Jorge
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Parkinson´s disease (PD) is a neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons from substantia nigra parscompacta (SNc). A genetic study identified 24 loci that are associated with PD; 11 of them are involved in/or disrupt various functions of theautophagic-lysosomal pathway. Lysosomes participate in the degradation of macromolecules from endocytosis and autophagy processes.Epidemiological and clinical studies reveal a difference in the development of PD between genders, giving sex hormones a neuroprotective functionand making them an interesting therapeutic proposal. The objective of this study is to analyze the effect of estrogens on the expression of lysosomalproteins in a rat model with the PD phenotype. Two-month-old male Sprague-Dawley rats were subjected to stereotaxic surgery to administer 6-hydroxydopamine (6-OHDA) or artificial cerebrospinal fluid (V) to the left striatum. After 7 days, they received chronic treatment for 10 days with17-β-estradiol (E) or V. The groups were made up of C (V lesion); E (V + E injury); HP (6-OHDA injury) and HPE (6-OHDA + E injury). After thetreatments, the animals were sacrificed, and the substantia nigra and prefrontal cortex were extracted and homogenized. Membranous and cytosolicfractions of the prefrontal cortex were obtained by differential centrifugation. The samples were processed for immunoblotting using antibodiesagainst cathepsin D (CatD) and actin. Preliminary results show that chronic treatment with estrogens increases the expression of CatD and actin inthe substantia nigra, and in the prefrontal cortex both proteins are increased in the cytosolic fraction. Since CatD reduces the α-synucleinconcentration in PD, the results suggest that an increase of the lysosomal function would exert neuroprotective action on cells affected by the disease.Likewise, it is worth mentioning that estrogens could also modulate the organization of the cytoskeleton, as a stage in neuromodulation.
Fil: Leiva, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Nodo Simulaciones Numericas, Modelado y Sistemas Complejos.; Argentina
Fil: Bonaccorso Marinelli, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Carvelli, Flavia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
IV Reunión Conjunta de Sociedades de Biología de la República Argentina
Mendoza
Argentina
Sociedad de Biología de Cuyo
Asociación de Biología de Tucumán
Sociedad de Biología de Córdoba
Sociedad de Biología de Rosario
Sociedad Argentina de Biología
Materia
PARKISON ' S DISEASE
CATHEPSIN D
ESTROGENS
LYSOSOMES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/200156
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/200156
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's diseaseLeiva, Natalia LorenaBonaccorso Marinelli, María PaulaCarvelli, Flavia LorenaSosa Escudero, Miguel AngelCabrera Kreiker, Ricardo JorgePARKISON ' S DISEASECATHEPSIN DESTROGENSLYSOSOMEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Parkinson´s disease (PD) is a neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons from substantia nigra parscompacta (SNc). A genetic study identified 24 loci that are associated with PD; 11 of them are involved in/or disrupt various functions of theautophagic-lysosomal pathway. Lysosomes participate in the degradation of macromolecules from endocytosis and autophagy processes.Epidemiological and clinical studies reveal a difference in the development of PD between genders, giving sex hormones a neuroprotective functionand making them an interesting therapeutic proposal. The objective of this study is to analyze the effect of estrogens on the expression of lysosomalproteins in a rat model with the PD phenotype. Two-month-old male Sprague-Dawley rats were subjected to stereotaxic surgery to administer 6-hydroxydopamine (6-OHDA) or artificial cerebrospinal fluid (V) to the left striatum. After 7 days, they received chronic treatment for 10 days with17-β-estradiol (E) or V. The groups were made up of C (V lesion); E (V + E injury); HP (6-OHDA injury) and HPE (6-OHDA + E injury). After thetreatments, the animals were sacrificed, and the substantia nigra and prefrontal cortex were extracted and homogenized. Membranous and cytosolicfractions of the prefrontal cortex were obtained by differential centrifugation. The samples were processed for immunoblotting using antibodiesagainst cathepsin D (CatD) and actin. Preliminary results show that chronic treatment with estrogens increases the expression of CatD and actin inthe substantia nigra, and in the prefrontal cortex both proteins are increased in the cytosolic fraction. Since CatD reduces the α-synucleinconcentration in PD, the results suggest that an increase of the lysosomal function would exert neuroprotective action on cells affected by the disease.Likewise, it is worth mentioning that estrogens could also modulate the organization of the cytoskeleton, as a stage in neuromodulation.Fil: Leiva, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Nodo Simulaciones Numericas, Modelado y Sistemas Complejos.; ArgentinaFil: Bonaccorso Marinelli, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Carvelli, Flavia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaIV Reunión Conjunta de Sociedades de Biología de la República ArgentinaMendozaArgentinaSociedad de Biología de CuyoAsociación de Biología de TucumánSociedad de Biología de CórdobaSociedad de Biología de RosarioSociedad Argentina de BiologíaTech Science Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/200156Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's disease; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; Mendoza; Argentina; 2020; 24-240327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.biologia.org.ar/jornadas-anteriores/info:eu-repo/semantics/altIdentifier/url/https://biologia.org.ar/downloads/BIOCELL-Vol_45-Suppl_3-2021.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:18:34Zoai:ri.conicet.gov.ar:11336/200156instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:18:34.343CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's disease
title Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's disease
spellingShingle Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's disease
Leiva, Natalia Lorena
PARKISON ' S DISEASE
CATHEPSIN D
ESTROGENS
LYSOSOMES
title_short Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's disease
title_full Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's disease
title_fullStr Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's disease
title_full_unstemmed Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's disease
title_sort Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's disease
dc.creator.none.fl_str_mv Leiva, Natalia Lorena
Bonaccorso Marinelli, María Paula
Carvelli, Flavia Lorena
Sosa Escudero, Miguel Angel
Cabrera Kreiker, Ricardo Jorge
author Leiva, Natalia Lorena
author_facet Leiva, Natalia Lorena
Bonaccorso Marinelli, María Paula
Carvelli, Flavia Lorena
Sosa Escudero, Miguel Angel
Cabrera Kreiker, Ricardo Jorge
author_role author
author2 Bonaccorso Marinelli, María Paula
Carvelli, Flavia Lorena
Sosa Escudero, Miguel Angel
Cabrera Kreiker, Ricardo Jorge
author2_role author
author
author
author
dc.subject.none.fl_str_mv PARKISON ' S DISEASE
CATHEPSIN D
ESTROGENS
LYSOSOMES
topic PARKISON ' S DISEASE
CATHEPSIN D
ESTROGENS
LYSOSOMES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Parkinson´s disease (PD) is a neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons from substantia nigra parscompacta (SNc). A genetic study identified 24 loci that are associated with PD; 11 of them are involved in/or disrupt various functions of theautophagic-lysosomal pathway. Lysosomes participate in the degradation of macromolecules from endocytosis and autophagy processes.Epidemiological and clinical studies reveal a difference in the development of PD between genders, giving sex hormones a neuroprotective functionand making them an interesting therapeutic proposal. The objective of this study is to analyze the effect of estrogens on the expression of lysosomalproteins in a rat model with the PD phenotype. Two-month-old male Sprague-Dawley rats were subjected to stereotaxic surgery to administer 6-hydroxydopamine (6-OHDA) or artificial cerebrospinal fluid (V) to the left striatum. After 7 days, they received chronic treatment for 10 days with17-β-estradiol (E) or V. The groups were made up of C (V lesion); E (V + E injury); HP (6-OHDA injury) and HPE (6-OHDA + E injury). After thetreatments, the animals were sacrificed, and the substantia nigra and prefrontal cortex were extracted and homogenized. Membranous and cytosolicfractions of the prefrontal cortex were obtained by differential centrifugation. The samples were processed for immunoblotting using antibodiesagainst cathepsin D (CatD) and actin. Preliminary results show that chronic treatment with estrogens increases the expression of CatD and actin inthe substantia nigra, and in the prefrontal cortex both proteins are increased in the cytosolic fraction. Since CatD reduces the α-synucleinconcentration in PD, the results suggest that an increase of the lysosomal function would exert neuroprotective action on cells affected by the disease.Likewise, it is worth mentioning that estrogens could also modulate the organization of the cytoskeleton, as a stage in neuromodulation.
Fil: Leiva, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Nodo Simulaciones Numericas, Modelado y Sistemas Complejos.; Argentina
Fil: Bonaccorso Marinelli, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Carvelli, Flavia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
IV Reunión Conjunta de Sociedades de Biología de la República Argentina
Mendoza
Argentina
Sociedad de Biología de Cuyo
Asociación de Biología de Tucumán
Sociedad de Biología de Córdoba
Sociedad de Biología de Rosario
Sociedad Argentina de Biología
description Parkinson´s disease (PD) is a neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons from substantia nigra parscompacta (SNc). A genetic study identified 24 loci that are associated with PD; 11 of them are involved in/or disrupt various functions of theautophagic-lysosomal pathway. Lysosomes participate in the degradation of macromolecules from endocytosis and autophagy processes.Epidemiological and clinical studies reveal a difference in the development of PD between genders, giving sex hormones a neuroprotective functionand making them an interesting therapeutic proposal. The objective of this study is to analyze the effect of estrogens on the expression of lysosomalproteins in a rat model with the PD phenotype. Two-month-old male Sprague-Dawley rats were subjected to stereotaxic surgery to administer 6-hydroxydopamine (6-OHDA) or artificial cerebrospinal fluid (V) to the left striatum. After 7 days, they received chronic treatment for 10 days with17-β-estradiol (E) or V. The groups were made up of C (V lesion); E (V + E injury); HP (6-OHDA injury) and HPE (6-OHDA + E injury). After thetreatments, the animals were sacrificed, and the substantia nigra and prefrontal cortex were extracted and homogenized. Membranous and cytosolicfractions of the prefrontal cortex were obtained by differential centrifugation. The samples were processed for immunoblotting using antibodiesagainst cathepsin D (CatD) and actin. Preliminary results show that chronic treatment with estrogens increases the expression of CatD and actin inthe substantia nigra, and in the prefrontal cortex both proteins are increased in the cytosolic fraction. Since CatD reduces the α-synucleinconcentration in PD, the results suggest that an increase of the lysosomal function would exert neuroprotective action on cells affected by the disease.Likewise, it is worth mentioning that estrogens could also modulate the organization of the cytoskeleton, as a stage in neuromodulation.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/200156
Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's disease; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; Mendoza; Argentina; 2020; 24-24
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/200156
identifier_str_mv Estrogens modulate expression of cathepsin D and actin in a rat model of parkinson's disease; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; Mendoza; Argentina; 2020; 24-24
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.biologia.org.ar/jornadas-anteriores/
info:eu-repo/semantics/altIdentifier/url/https://biologia.org.ar/downloads/BIOCELL-Vol_45-Suppl_3-2021.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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