Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposes

Autores
Kulik, Tomasz; Brankovics, Balazs; Van Diepeningen, Anne D.; Bilska, Katarzyna; Zelechowski, Maciej; Myszczyński, Kamil; Molcan, Tomasz; Stakheev. Alexander; Stenglein, Sebastian Alberto; Beyer, Marco; Pasquali, Matias; Sawicki, Jakub; Baturo Cieśniewska, Anna
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Much of the mitogenome variation observed in fungal lineages seems driven by mobile genetic elements (MGEs), which have invaded their genomes throughout evolution. The variation in the distribution and nucleotide diversity of these elements appears to be the main distinction between different fungal taxa, making them promising candidates for diagnostic purposes. Fungi of the genus Fusarium display a high variation in MGE content, from MGE-poor (F. oxysporum and Fusarium fujikuroi species complex) to MGE-rich mitogenomes found in the important cereal pathogens F. culmorum and F. graminearum sensu stricto. In this study, we investigated the MGE variation in these latter two species by mitogenome analysis of geographically diverse strains. In addition, a smaller set of F. cerealis and F. pseudograminearum strains was included for comparison. Forty-seven introns harboring from 0 to 3 endonucleases (HEGs) were identified in the standard set of mitochondrial protein-coding genes. Most of them belonged to the group I intron family and harbored either LAGLIDADG or GIY-YIG HEGs. Among a total of 53 HEGs, 27 were shared by all fungal strains. Most of the optional HEGs were irregularly distributed among fungal strains/species indicating ancestral mosaicism in MGEs. However, among optional MGEs, one exhibited species-specific conservation in F. culmorum. While in F. graminearum s.s. MGE patterns in cox3 and in the intergenic spacer between cox2 and nad4L may facilitate the identification of this species. Thus, our results demonstrate distinctive traits of mitogenomes for diagnostic purposes of Fusaria.
Fil: Kulik, Tomasz. Department Of Botany And Nature Protection, University; Polonia
Fil: Brankovics, Balazs. Wageningen Plant Research, Wageningen University; Países Bajos
Fil: Van Diepeningen, Anne D.. Waneningen Plant Research; Países Bajos
Fil: Bilska, Katarzyna. Department Of Botany And Nature Protection, University; Polonia
Fil: Zelechowski, Maciej. Department Of Botany And Nature Protection, University; Polonia
Fil: Myszczyński, Kamil. Department Of Botany And Nature Protection, University; Polonia
Fil: Molcan, Tomasz. Faculty Of Biology And Biotechnology, University; Polonia
Fil: Stakheev. Alexander. Institute Of Bioorganic Chemistry (ras); Rusia
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Beyer, Marco. Luxembourg Institute Of Science And Technology; Luxemburgo
Fil: Pasquali, Matias. Faculty Of Agricultural And Food Sciences; Italia
Fil: Sawicki, Jakub. Department Of Botany And Nature Protection, University; Polonia
Fil: Baturo Cieśniewska, Anna. Baturo-cieśniewska; Polonia
Materia
FUSARIUM SPECIES
MITOGENOME
MITOCHONDRIAL INTRONS
HOMING ENDONUCLEASES
MOBIL GENETICS ELEMENTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/107090
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/107090
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposesKulik, TomaszBrankovics, BalazsVan Diepeningen, Anne D.Bilska, KatarzynaZelechowski, MaciejMyszczyński, KamilMolcan, TomaszStakheev. AlexanderStenglein, Sebastian AlbertoBeyer, MarcoPasquali, MatiasSawicki, JakubBaturo Cieśniewska, AnnaFUSARIUM SPECIESMITOGENOMEMITOCHONDRIAL INTRONSHOMING ENDONUCLEASESMOBIL GENETICS ELEMENTShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Much of the mitogenome variation observed in fungal lineages seems driven by mobile genetic elements (MGEs), which have invaded their genomes throughout evolution. The variation in the distribution and nucleotide diversity of these elements appears to be the main distinction between different fungal taxa, making them promising candidates for diagnostic purposes. Fungi of the genus Fusarium display a high variation in MGE content, from MGE-poor (F. oxysporum and Fusarium fujikuroi species complex) to MGE-rich mitogenomes found in the important cereal pathogens F. culmorum and F. graminearum sensu stricto. In this study, we investigated the MGE variation in these latter two species by mitogenome analysis of geographically diverse strains. In addition, a smaller set of F. cerealis and F. pseudograminearum strains was included for comparison. Forty-seven introns harboring from 0 to 3 endonucleases (HEGs) were identified in the standard set of mitochondrial protein-coding genes. Most of them belonged to the group I intron family and harbored either LAGLIDADG or GIY-YIG HEGs. Among a total of 53 HEGs, 27 were shared by all fungal strains. Most of the optional HEGs were irregularly distributed among fungal strains/species indicating ancestral mosaicism in MGEs. However, among optional MGEs, one exhibited species-specific conservation in F. culmorum. While in F. graminearum s.s. MGE patterns in cox3 and in the intergenic spacer between cox2 and nad4L may facilitate the identification of this species. Thus, our results demonstrate distinctive traits of mitogenomes for diagnostic purposes of Fusaria.Fil: Kulik, Tomasz. Department Of Botany And Nature Protection, University; PoloniaFil: Brankovics, Balazs. Wageningen Plant Research, Wageningen University; Países BajosFil: Van Diepeningen, Anne D.. Waneningen Plant Research; Países BajosFil: Bilska, Katarzyna. Department Of Botany And Nature Protection, University; PoloniaFil: Zelechowski, Maciej. Department Of Botany And Nature Protection, University; PoloniaFil: Myszczyński, Kamil. Department Of Botany And Nature Protection, University; PoloniaFil: Molcan, Tomasz. Faculty Of Biology And Biotechnology, University; PoloniaFil: Stakheev. Alexander. Institute Of Bioorganic Chemistry (ras); RusiaFil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaFil: Beyer, Marco. Luxembourg Institute Of Science And Technology; LuxemburgoFil: Pasquali, Matias. Faculty Of Agricultural And Food Sciences; ItaliaFil: Sawicki, Jakub. Department Of Botany And Nature Protection, University; PoloniaFil: Baturo Cieśniewska, Anna. Baturo-cieśniewska; PoloniaFrontiers2020-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/107090Kulik, Tomasz; Brankovics, Balazs; Van Diepeningen, Anne D.; Bilska, Katarzyna; Zelechowski, Maciej; et al.; Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposes; Frontiers; Frontiers in Microbiology; 4-2020; 1-141664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.01002info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2020.01002/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:17:45Zoai:ri.conicet.gov.ar:11336/107090instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:17:45.434CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposes
title Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposes
spellingShingle Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposes
Kulik, Tomasz
FUSARIUM SPECIES
MITOGENOME
MITOCHONDRIAL INTRONS
HOMING ENDONUCLEASES
MOBIL GENETICS ELEMENTS
title_short Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposes
title_full Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposes
title_fullStr Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposes
title_full_unstemmed Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposes
title_sort Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposes
dc.creator.none.fl_str_mv Kulik, Tomasz
Brankovics, Balazs
Van Diepeningen, Anne D.
Bilska, Katarzyna
Zelechowski, Maciej
Myszczyński, Kamil
Molcan, Tomasz
Stakheev. Alexander
Stenglein, Sebastian Alberto
Beyer, Marco
Pasquali, Matias
Sawicki, Jakub
Baturo Cieśniewska, Anna
author Kulik, Tomasz
author_facet Kulik, Tomasz
Brankovics, Balazs
Van Diepeningen, Anne D.
Bilska, Katarzyna
Zelechowski, Maciej
Myszczyński, Kamil
Molcan, Tomasz
Stakheev. Alexander
Stenglein, Sebastian Alberto
Beyer, Marco
Pasquali, Matias
Sawicki, Jakub
Baturo Cieśniewska, Anna
author_role author
author2 Brankovics, Balazs
Van Diepeningen, Anne D.
Bilska, Katarzyna
Zelechowski, Maciej
Myszczyński, Kamil
Molcan, Tomasz
Stakheev. Alexander
Stenglein, Sebastian Alberto
Beyer, Marco
Pasquali, Matias
Sawicki, Jakub
Baturo Cieśniewska, Anna
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FUSARIUM SPECIES
MITOGENOME
MITOCHONDRIAL INTRONS
HOMING ENDONUCLEASES
MOBIL GENETICS ELEMENTS
topic FUSARIUM SPECIES
MITOGENOME
MITOCHONDRIAL INTRONS
HOMING ENDONUCLEASES
MOBIL GENETICS ELEMENTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Much of the mitogenome variation observed in fungal lineages seems driven by mobile genetic elements (MGEs), which have invaded their genomes throughout evolution. The variation in the distribution and nucleotide diversity of these elements appears to be the main distinction between different fungal taxa, making them promising candidates for diagnostic purposes. Fungi of the genus Fusarium display a high variation in MGE content, from MGE-poor (F. oxysporum and Fusarium fujikuroi species complex) to MGE-rich mitogenomes found in the important cereal pathogens F. culmorum and F. graminearum sensu stricto. In this study, we investigated the MGE variation in these latter two species by mitogenome analysis of geographically diverse strains. In addition, a smaller set of F. cerealis and F. pseudograminearum strains was included for comparison. Forty-seven introns harboring from 0 to 3 endonucleases (HEGs) were identified in the standard set of mitochondrial protein-coding genes. Most of them belonged to the group I intron family and harbored either LAGLIDADG or GIY-YIG HEGs. Among a total of 53 HEGs, 27 were shared by all fungal strains. Most of the optional HEGs were irregularly distributed among fungal strains/species indicating ancestral mosaicism in MGEs. However, among optional MGEs, one exhibited species-specific conservation in F. culmorum. While in F. graminearum s.s. MGE patterns in cox3 and in the intergenic spacer between cox2 and nad4L may facilitate the identification of this species. Thus, our results demonstrate distinctive traits of mitogenomes for diagnostic purposes of Fusaria.
Fil: Kulik, Tomasz. Department Of Botany And Nature Protection, University; Polonia
Fil: Brankovics, Balazs. Wageningen Plant Research, Wageningen University; Países Bajos
Fil: Van Diepeningen, Anne D.. Waneningen Plant Research; Países Bajos
Fil: Bilska, Katarzyna. Department Of Botany And Nature Protection, University; Polonia
Fil: Zelechowski, Maciej. Department Of Botany And Nature Protection, University; Polonia
Fil: Myszczyński, Kamil. Department Of Botany And Nature Protection, University; Polonia
Fil: Molcan, Tomasz. Faculty Of Biology And Biotechnology, University; Polonia
Fil: Stakheev. Alexander. Institute Of Bioorganic Chemistry (ras); Rusia
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Beyer, Marco. Luxembourg Institute Of Science And Technology; Luxemburgo
Fil: Pasquali, Matias. Faculty Of Agricultural And Food Sciences; Italia
Fil: Sawicki, Jakub. Department Of Botany And Nature Protection, University; Polonia
Fil: Baturo Cieśniewska, Anna. Baturo-cieśniewska; Polonia
description Much of the mitogenome variation observed in fungal lineages seems driven by mobile genetic elements (MGEs), which have invaded their genomes throughout evolution. The variation in the distribution and nucleotide diversity of these elements appears to be the main distinction between different fungal taxa, making them promising candidates for diagnostic purposes. Fungi of the genus Fusarium display a high variation in MGE content, from MGE-poor (F. oxysporum and Fusarium fujikuroi species complex) to MGE-rich mitogenomes found in the important cereal pathogens F. culmorum and F. graminearum sensu stricto. In this study, we investigated the MGE variation in these latter two species by mitogenome analysis of geographically diverse strains. In addition, a smaller set of F. cerealis and F. pseudograminearum strains was included for comparison. Forty-seven introns harboring from 0 to 3 endonucleases (HEGs) were identified in the standard set of mitochondrial protein-coding genes. Most of them belonged to the group I intron family and harbored either LAGLIDADG or GIY-YIG HEGs. Among a total of 53 HEGs, 27 were shared by all fungal strains. Most of the optional HEGs were irregularly distributed among fungal strains/species indicating ancestral mosaicism in MGEs. However, among optional MGEs, one exhibited species-specific conservation in F. culmorum. While in F. graminearum s.s. MGE patterns in cox3 and in the intergenic spacer between cox2 and nad4L may facilitate the identification of this species. Thus, our results demonstrate distinctive traits of mitogenomes for diagnostic purposes of Fusaria.
publishDate 2020
dc.date.none.fl_str_mv 2020-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/107090
Kulik, Tomasz; Brankovics, Balazs; Van Diepeningen, Anne D.; Bilska, Katarzyna; Zelechowski, Maciej; et al.; Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposes; Frontiers; Frontiers in Microbiology; 4-2020; 1-14
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/107090
identifier_str_mv Kulik, Tomasz; Brankovics, Balazs; Van Diepeningen, Anne D.; Bilska, Katarzyna; Zelechowski, Maciej; et al.; Diversity of mobile genetic elements in the mitogenome of closely related Fusarium culmorum and F. graminearum sensu stricto strains ans its implication for diagnostic purposes; Frontiers; Frontiers in Microbiology; 4-2020; 1-14
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.01002
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2020.01002/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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