Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neurons

Autores
Heidenreich, Matthias; Tzingounis, Anastassios V.; Kharkovets, Tatjana; Spitzmaul, Guillermo Federico; Nicoll, Roger A.; Jentsch, Thomas J.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Benign familial neonatal convulsion (BNFC) is a neurological disorder caused by mutations in the potassium channel genes KCNQ2 and KCNQ3, which contribute to the medium afterhyperpolarization current (ImAHP) and slow afterhyperpolarization current (IsAHP) in hippocampal neurons. KCNQ5 is not yet linked to any human disease but is broadly expressed in the brain similar to KCNQ2 and KCNQ3. To investigate the role of KCNQ5 in the brain we generated a KCNQ5 dominantnegative (Kcnq5dn/dn) mouse. Histological analysis did not reveal structural brain abnormalities. Western blots of total brain proteins revealed that there is no detectable influence of the mutated KCNQ5 protein on overall KCNQ2, KCNQ3 and KCNQ5 levels. In addition, the subcellular localization of KCNQ2 and KCNQ3 is not altered in Kcnq5dn/dn mice. Using electrophysiological analysis we could show that KCNQ5 contributes to the ImAHP and IsAHP in a subset of hippocampal neurons where KCNQ5 is highly expressed. Therefore, our study is a direct demonstration that in addition to KCNQ2 and KCNQ3, KCNQ5 channels contribute to the ImAHP and IsAHP.
Fil: Heidenreich, Matthias. Max Delbruk Center For Molecular Medicine; Alemania. Humboldt-Universität zu Berlin; Alemania
Fil: Tzingounis, Anastassios V.. University of California; Estados Unidos
Fil: Kharkovets, Tatjana. Max Delbruk Center For Molecular Medicine; Alemania. Humboldt-Universität zu Berlin; Alemania
Fil: Spitzmaul, Guillermo Federico. Max Delbruk Center For Molecular Medicine; Alemania. Humboldt-Universität zu Berlin; Alemania
Fil: Nicoll, Roger A.. University of California; Estados Unidos
Fil: Jentsch, Thomas J.. Max Delbruk Center For Molecular Medicine; Alemania. Humboldt-Universität zu Berlin; Alemania
Berlin Brain Days 2009
Berlin
Alemania
Berlin School of Mind and Brain
Humboldt-Universität zu Berlin
Materia
KCNQ5
HIPPOCAMPUS
AFTERHYPERPOLARIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242325
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neuronsHeidenreich, MatthiasTzingounis, Anastassios V.Kharkovets, TatjanaSpitzmaul, Guillermo FedericoNicoll, Roger A.Jentsch, Thomas J.KCNQ5HIPPOCAMPUSAFTERHYPERPOLARIZATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Benign familial neonatal convulsion (BNFC) is a neurological disorder caused by mutations in the potassium channel genes KCNQ2 and KCNQ3, which contribute to the medium afterhyperpolarization current (ImAHP) and slow afterhyperpolarization current (IsAHP) in hippocampal neurons. KCNQ5 is not yet linked to any human disease but is broadly expressed in the brain similar to KCNQ2 and KCNQ3. To investigate the role of KCNQ5 in the brain we generated a KCNQ5 dominantnegative (Kcnq5dn/dn) mouse. Histological analysis did not reveal structural brain abnormalities. Western blots of total brain proteins revealed that there is no detectable influence of the mutated KCNQ5 protein on overall KCNQ2, KCNQ3 and KCNQ5 levels. In addition, the subcellular localization of KCNQ2 and KCNQ3 is not altered in Kcnq5dn/dn mice. Using electrophysiological analysis we could show that KCNQ5 contributes to the ImAHP and IsAHP in a subset of hippocampal neurons where KCNQ5 is highly expressed. Therefore, our study is a direct demonstration that in addition to KCNQ2 and KCNQ3, KCNQ5 channels contribute to the ImAHP and IsAHP.Fil: Heidenreich, Matthias. Max Delbruk Center For Molecular Medicine; Alemania. Humboldt-Universität zu Berlin; AlemaniaFil: Tzingounis, Anastassios V.. University of California; Estados UnidosFil: Kharkovets, Tatjana. Max Delbruk Center For Molecular Medicine; Alemania. Humboldt-Universität zu Berlin; AlemaniaFil: Spitzmaul, Guillermo Federico. Max Delbruk Center For Molecular Medicine; Alemania. Humboldt-Universität zu Berlin; AlemaniaFil: Nicoll, Roger A.. University of California; Estados UnidosFil: Jentsch, Thomas J.. Max Delbruk Center For Molecular Medicine; Alemania. Humboldt-Universität zu Berlin; AlemaniaBerlin Brain Days 2009BerlinAlemaniaBerlin School of Mind and BrainHumboldt-Universität zu BerlinMax Delbrück Centrum für Molekulare Medizin2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242325Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neurons; Berlin Brain Days 2009; Berlin; Alemania; 2009; 67-67CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mind-and-brain.de/news/detail/berlin-brain-daysNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:12:21Zoai:ri.conicet.gov.ar:11336/242325instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:12:21.348CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neurons
title Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neurons
spellingShingle Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neurons
Heidenreich, Matthias
KCNQ5
HIPPOCAMPUS
AFTERHYPERPOLARIZATION
title_short Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neurons
title_full Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neurons
title_fullStr Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neurons
title_full_unstemmed Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neurons
title_sort Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neurons
dc.creator.none.fl_str_mv Heidenreich, Matthias
Tzingounis, Anastassios V.
Kharkovets, Tatjana
Spitzmaul, Guillermo Federico
Nicoll, Roger A.
Jentsch, Thomas J.
author Heidenreich, Matthias
author_facet Heidenreich, Matthias
Tzingounis, Anastassios V.
Kharkovets, Tatjana
Spitzmaul, Guillermo Federico
Nicoll, Roger A.
Jentsch, Thomas J.
author_role author
author2 Tzingounis, Anastassios V.
Kharkovets, Tatjana
Spitzmaul, Guillermo Federico
Nicoll, Roger A.
Jentsch, Thomas J.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv KCNQ5
HIPPOCAMPUS
AFTERHYPERPOLARIZATION
topic KCNQ5
HIPPOCAMPUS
AFTERHYPERPOLARIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Benign familial neonatal convulsion (BNFC) is a neurological disorder caused by mutations in the potassium channel genes KCNQ2 and KCNQ3, which contribute to the medium afterhyperpolarization current (ImAHP) and slow afterhyperpolarization current (IsAHP) in hippocampal neurons. KCNQ5 is not yet linked to any human disease but is broadly expressed in the brain similar to KCNQ2 and KCNQ3. To investigate the role of KCNQ5 in the brain we generated a KCNQ5 dominantnegative (Kcnq5dn/dn) mouse. Histological analysis did not reveal structural brain abnormalities. Western blots of total brain proteins revealed that there is no detectable influence of the mutated KCNQ5 protein on overall KCNQ2, KCNQ3 and KCNQ5 levels. In addition, the subcellular localization of KCNQ2 and KCNQ3 is not altered in Kcnq5dn/dn mice. Using electrophysiological analysis we could show that KCNQ5 contributes to the ImAHP and IsAHP in a subset of hippocampal neurons where KCNQ5 is highly expressed. Therefore, our study is a direct demonstration that in addition to KCNQ2 and KCNQ3, KCNQ5 channels contribute to the ImAHP and IsAHP.
Fil: Heidenreich, Matthias. Max Delbruk Center For Molecular Medicine; Alemania. Humboldt-Universität zu Berlin; Alemania
Fil: Tzingounis, Anastassios V.. University of California; Estados Unidos
Fil: Kharkovets, Tatjana. Max Delbruk Center For Molecular Medicine; Alemania. Humboldt-Universität zu Berlin; Alemania
Fil: Spitzmaul, Guillermo Federico. Max Delbruk Center For Molecular Medicine; Alemania. Humboldt-Universität zu Berlin; Alemania
Fil: Nicoll, Roger A.. University of California; Estados Unidos
Fil: Jentsch, Thomas J.. Max Delbruk Center For Molecular Medicine; Alemania. Humboldt-Universität zu Berlin; Alemania
Berlin Brain Days 2009
Berlin
Alemania
Berlin School of Mind and Brain
Humboldt-Universität zu Berlin
description Benign familial neonatal convulsion (BNFC) is a neurological disorder caused by mutations in the potassium channel genes KCNQ2 and KCNQ3, which contribute to the medium afterhyperpolarization current (ImAHP) and slow afterhyperpolarization current (IsAHP) in hippocampal neurons. KCNQ5 is not yet linked to any human disease but is broadly expressed in the brain similar to KCNQ2 and KCNQ3. To investigate the role of KCNQ5 in the brain we generated a KCNQ5 dominantnegative (Kcnq5dn/dn) mouse. Histological analysis did not reveal structural brain abnormalities. Western blots of total brain proteins revealed that there is no detectable influence of the mutated KCNQ5 protein on overall KCNQ2, KCNQ3 and KCNQ5 levels. In addition, the subcellular localization of KCNQ2 and KCNQ3 is not altered in Kcnq5dn/dn mice. Using electrophysiological analysis we could show that KCNQ5 contributes to the ImAHP and IsAHP in a subset of hippocampal neurons where KCNQ5 is highly expressed. Therefore, our study is a direct demonstration that in addition to KCNQ2 and KCNQ3, KCNQ5 channels contribute to the ImAHP and IsAHP.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Simposio
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242325
Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neurons; Berlin Brain Days 2009; Berlin; Alemania; 2009; 67-67
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242325
identifier_str_mv Contribution of KCNQ5 to the medium and slow afterhyperpolarization currents in hippocampal neurons; Berlin Brain Days 2009; Berlin; Alemania; 2009; 67-67
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mind-and-brain.de/news/detail/berlin-brain-days
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Max Delbrück Centrum für Molekulare Medizin
publisher.none.fl_str_mv Max Delbrück Centrum für Molekulare Medizin
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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