Mismatch recognition function of Arabidopsis thaliana MutS-gamma

Autores
Gomez, Rodrigo Lionel; Spampinato, Claudia Patricia
Año de publicación
2013
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Genetic stability depends in part on an efficient DNA lesion recognition and correction by the DNA mismatch repair (MMR) system. In eukaryotes, MMR is initiated by the binding of heterodimeric MutS homologue (MSH) complexes, MSH2/MSH6 and MSH2/MSH3, which recognize and bind mismatches and unpaired nucleotides. Plants encode another mismatch recognition protein, named MSH7. MSH7 forms a heterodimer with MSH2 and the protein complex is designated MutS-gamma. We here report the effect the expression of Arabidopsis MSH2 and MSH7 alone or in combination exert on the genomic stability of Saccharomyces cerevisiae. AtMSH2 and AtMutS-gamma proteins failed to complement the hypermutator phenotype of an msh2 deficient strain. However, overexpressing AtMutS-gamma in MMR proficient strains generated a 4-fold increase in CAN1 forward mutation rate, when compared to wild-type strains. CanR mutation spectrum analysis of AtMutS-gamma overproducing strains revealed a substantial increase in the frequency of base substitution mutations, including an increased accumulation of base pair changes from G:C to A:T and T:A to C:G, G:C or A:T. Taken together, these results suggest that AtMutS-gamma affects yeast genomic stability by recognizing specific mismatches and preventing correction by yeast MutS-alpha and MutS-beta, with subsequent inability to interact with yeast downstream proteins needed to complete MMR.
Fil: Gomez, Rodrigo Lionel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina
Fil: Spampinato, Claudia Patricia. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Materia
Mismatch Repair
Genome Stability
Mutation Rate
Arabidopsis Thaliana
Saccharomyces Cerevisiae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3420
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/3420
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mismatch recognition function of Arabidopsis thaliana MutS-gammaGomez, Rodrigo LionelSpampinato, Claudia PatriciaMismatch RepairGenome StabilityMutation RateArabidopsis ThalianaSaccharomyces Cerevisiaehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Genetic stability depends in part on an efficient DNA lesion recognition and correction by the DNA mismatch repair (MMR) system. In eukaryotes, MMR is initiated by the binding of heterodimeric MutS homologue (MSH) complexes, MSH2/MSH6 and MSH2/MSH3, which recognize and bind mismatches and unpaired nucleotides. Plants encode another mismatch recognition protein, named MSH7. MSH7 forms a heterodimer with MSH2 and the protein complex is designated MutS-gamma. We here report the effect the expression of Arabidopsis MSH2 and MSH7 alone or in combination exert on the genomic stability of Saccharomyces cerevisiae. AtMSH2 and AtMutS-gamma proteins failed to complement the hypermutator phenotype of an msh2 deficient strain. However, overexpressing AtMutS-gamma in MMR proficient strains generated a 4-fold increase in CAN1 forward mutation rate, when compared to wild-type strains. CanR mutation spectrum analysis of AtMutS-gamma overproducing strains revealed a substantial increase in the frequency of base substitution mutations, including an increased accumulation of base pair changes from G:C to A:T and T:A to C:G, G:C or A:T. Taken together, these results suggest that AtMutS-gamma affects yeast genomic stability by recognizing specific mismatches and preventing correction by yeast MutS-alpha and MutS-beta, with subsequent inability to interact with yeast downstream proteins needed to complete MMR.Fil: Gomez, Rodrigo Lionel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; ArgentinaFil: Spampinato, Claudia Patricia. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaElsevier Science2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3420Gomez, Rodrigo Lionel; Spampinato, Claudia Patricia; Mismatch recognition function of Arabidopsis thaliana MutS-gamma; Elsevier Science; Dna Repair; 12; 2-2013; 257-2641568-7864spainfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1568786413000189info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:46Zoai:ri.conicet.gov.ar:11336/3420instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:46.918CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mismatch recognition function of Arabidopsis thaliana MutS-gamma
title Mismatch recognition function of Arabidopsis thaliana MutS-gamma
spellingShingle Mismatch recognition function of Arabidopsis thaliana MutS-gamma
Gomez, Rodrigo Lionel
Mismatch Repair
Genome Stability
Mutation Rate
Arabidopsis Thaliana
Saccharomyces Cerevisiae
title_short Mismatch recognition function of Arabidopsis thaliana MutS-gamma
title_full Mismatch recognition function of Arabidopsis thaliana MutS-gamma
title_fullStr Mismatch recognition function of Arabidopsis thaliana MutS-gamma
title_full_unstemmed Mismatch recognition function of Arabidopsis thaliana MutS-gamma
title_sort Mismatch recognition function of Arabidopsis thaliana MutS-gamma
dc.creator.none.fl_str_mv Gomez, Rodrigo Lionel
Spampinato, Claudia Patricia
author Gomez, Rodrigo Lionel
author_facet Gomez, Rodrigo Lionel
Spampinato, Claudia Patricia
author_role author
author2 Spampinato, Claudia Patricia
author2_role author
dc.subject.none.fl_str_mv Mismatch Repair
Genome Stability
Mutation Rate
Arabidopsis Thaliana
Saccharomyces Cerevisiae
topic Mismatch Repair
Genome Stability
Mutation Rate
Arabidopsis Thaliana
Saccharomyces Cerevisiae
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Genetic stability depends in part on an efficient DNA lesion recognition and correction by the DNA mismatch repair (MMR) system. In eukaryotes, MMR is initiated by the binding of heterodimeric MutS homologue (MSH) complexes, MSH2/MSH6 and MSH2/MSH3, which recognize and bind mismatches and unpaired nucleotides. Plants encode another mismatch recognition protein, named MSH7. MSH7 forms a heterodimer with MSH2 and the protein complex is designated MutS-gamma. We here report the effect the expression of Arabidopsis MSH2 and MSH7 alone or in combination exert on the genomic stability of Saccharomyces cerevisiae. AtMSH2 and AtMutS-gamma proteins failed to complement the hypermutator phenotype of an msh2 deficient strain. However, overexpressing AtMutS-gamma in MMR proficient strains generated a 4-fold increase in CAN1 forward mutation rate, when compared to wild-type strains. CanR mutation spectrum analysis of AtMutS-gamma overproducing strains revealed a substantial increase in the frequency of base substitution mutations, including an increased accumulation of base pair changes from G:C to A:T and T:A to C:G, G:C or A:T. Taken together, these results suggest that AtMutS-gamma affects yeast genomic stability by recognizing specific mismatches and preventing correction by yeast MutS-alpha and MutS-beta, with subsequent inability to interact with yeast downstream proteins needed to complete MMR.
Fil: Gomez, Rodrigo Lionel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina
Fil: Spampinato, Claudia Patricia. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
description Genetic stability depends in part on an efficient DNA lesion recognition and correction by the DNA mismatch repair (MMR) system. In eukaryotes, MMR is initiated by the binding of heterodimeric MutS homologue (MSH) complexes, MSH2/MSH6 and MSH2/MSH3, which recognize and bind mismatches and unpaired nucleotides. Plants encode another mismatch recognition protein, named MSH7. MSH7 forms a heterodimer with MSH2 and the protein complex is designated MutS-gamma. We here report the effect the expression of Arabidopsis MSH2 and MSH7 alone or in combination exert on the genomic stability of Saccharomyces cerevisiae. AtMSH2 and AtMutS-gamma proteins failed to complement the hypermutator phenotype of an msh2 deficient strain. However, overexpressing AtMutS-gamma in MMR proficient strains generated a 4-fold increase in CAN1 forward mutation rate, when compared to wild-type strains. CanR mutation spectrum analysis of AtMutS-gamma overproducing strains revealed a substantial increase in the frequency of base substitution mutations, including an increased accumulation of base pair changes from G:C to A:T and T:A to C:G, G:C or A:T. Taken together, these results suggest that AtMutS-gamma affects yeast genomic stability by recognizing specific mismatches and preventing correction by yeast MutS-alpha and MutS-beta, with subsequent inability to interact with yeast downstream proteins needed to complete MMR.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3420
Gomez, Rodrigo Lionel; Spampinato, Claudia Patricia; Mismatch recognition function of Arabidopsis thaliana MutS-gamma; Elsevier Science; Dna Repair; 12; 2-2013; 257-264
1568-7864
url http://hdl.handle.net/11336/3420
identifier_str_mv Gomez, Rodrigo Lionel; Spampinato, Claudia Patricia; Mismatch recognition function of Arabidopsis thaliana MutS-gamma; Elsevier Science; Dna Repair; 12; 2-2013; 257-264
1568-7864
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1568786413000189
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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