Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination

Autores
Lario, Luciana Daniela; Botta, Pablo Eduardo; Casati, Paula; Spampinato, Claudia Patricia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The mismatch repair (MMR) system maintains genome integrity by correcting replication associated errors and inhibiting recombination between divergent DNA sequences. The basic features of the pathway have been highly conserved throughout evolution, although the nature and number of the proteins involved in this DNA repair system vary among organisms. Plants have an extra mismatch recognition protein, named MutS. The protein is a heterodimer of MSH2?MSH7. To further understand the in vivo role of MSH7, we present data from this protein in Arabidopsis thaliana plants. First, we generated transgenic plants that express the β-glucuronidase (GUS) under the control of the MSH7 promoter. Histochemical staining of the transgenic plants indicated that MSH7 is preferentially expressed in proliferating tissues. Then, we identified msh7 T-DNA insertion mutants. Plants deficient in MSH7 show increased levels of UV-B-induced cyclobutane pyrimidine dimers (CPDs) relative to wild-type (WT) plants. Consistent with the patterns of MSH7 expression, we next analyzed the role of the protein during somatic and meiotic recombination. The frequency of somatic recombination between homologous or homeologous repeats (divergence level of 1.6%) was monitored using a previously described GUS recombination reporter assay. Disruption of MSH7 has no effect on the rates of somatic homologous or homeologous recombination under control conditions or after UV-B exposure. However, the rate of meiotic recombination between two genetically linked seed-specific fluorescent markers was 97% higher in msh7 than in WT plants. Taken together, these results suggest that MSH7 is involved in UV-B-induced DNA damage recognition and in controlling meiotic recombination.
Fil: Lario, Luciana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Botta, Pablo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Materia
Arabidopsis Thaliana
Meiotic Recombination
Mismatch Repair
Mitotic Recombination
Sequence Divergence
Uv-B Radiation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7855
Acceder
id CONICETDig_d42eed04fadc716e57ccb8785b0b3be1
oai_identifier_str oai:ri.conicet.gov.ar:11336/7855
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of AtMSH7 in UV-B-induced DNA damage recognition and recombinationLario, Luciana DanielaBotta, Pablo EduardoCasati, PaulaSpampinato, Claudia PatriciaArabidopsis ThalianaMeiotic RecombinationMismatch RepairMitotic RecombinationSequence DivergenceUv-B Radiationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The mismatch repair (MMR) system maintains genome integrity by correcting replication associated errors and inhibiting recombination between divergent DNA sequences. The basic features of the pathway have been highly conserved throughout evolution, although the nature and number of the proteins involved in this DNA repair system vary among organisms. Plants have an extra mismatch recognition protein, named MutS. The protein is a heterodimer of MSH2?MSH7. To further understand the in vivo role of MSH7, we present data from this protein in Arabidopsis thaliana plants. First, we generated transgenic plants that express the β-glucuronidase (GUS) under the control of the MSH7 promoter. Histochemical staining of the transgenic plants indicated that MSH7 is preferentially expressed in proliferating tissues. Then, we identified msh7 T-DNA insertion mutants. Plants deficient in MSH7 show increased levels of UV-B-induced cyclobutane pyrimidine dimers (CPDs) relative to wild-type (WT) plants. Consistent with the patterns of MSH7 expression, we next analyzed the role of the protein during somatic and meiotic recombination. The frequency of somatic recombination between homologous or homeologous repeats (divergence level of 1.6%) was monitored using a previously described GUS recombination reporter assay. Disruption of MSH7 has no effect on the rates of somatic homologous or homeologous recombination under control conditions or after UV-B exposure. However, the rate of meiotic recombination between two genetically linked seed-specific fluorescent markers was 97% higher in msh7 than in WT plants. Taken together, these results suggest that MSH7 is involved in UV-B-induced DNA damage recognition and in controlling meiotic recombination.Fil: Lario, Luciana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Botta, Pablo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaOxford University Press2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7855Lario, Luciana Daniela; Botta, Pablo Eduardo; Casati, Paula; Spampinato, Claudia Patricia; Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination; Oxford University Press; Journal Of Experimental Botany; 66; 11; 12-2014; 3019-30260022-0957enginfo:eu-repo/semantics/altIdentifier/url/http://jxb.oxfordjournals.org/content/66/11/3019.abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/eru464info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:44:44Zoai:ri.conicet.gov.ar:11336/7855instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:44:45.146CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination
title Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination
spellingShingle Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination
Lario, Luciana Daniela
Arabidopsis Thaliana
Meiotic Recombination
Mismatch Repair
Mitotic Recombination
Sequence Divergence
Uv-B Radiation
title_short Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination
title_full Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination
title_fullStr Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination
title_full_unstemmed Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination
title_sort Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination
dc.creator.none.fl_str_mv Lario, Luciana Daniela
Botta, Pablo Eduardo
Casati, Paula
Spampinato, Claudia Patricia
author Lario, Luciana Daniela
author_facet Lario, Luciana Daniela
Botta, Pablo Eduardo
Casati, Paula
Spampinato, Claudia Patricia
author_role author
author2 Botta, Pablo Eduardo
Casati, Paula
Spampinato, Claudia Patricia
author2_role author
author
author
dc.subject.none.fl_str_mv Arabidopsis Thaliana
Meiotic Recombination
Mismatch Repair
Mitotic Recombination
Sequence Divergence
Uv-B Radiation
topic Arabidopsis Thaliana
Meiotic Recombination
Mismatch Repair
Mitotic Recombination
Sequence Divergence
Uv-B Radiation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The mismatch repair (MMR) system maintains genome integrity by correcting replication associated errors and inhibiting recombination between divergent DNA sequences. The basic features of the pathway have been highly conserved throughout evolution, although the nature and number of the proteins involved in this DNA repair system vary among organisms. Plants have an extra mismatch recognition protein, named MutS. The protein is a heterodimer of MSH2?MSH7. To further understand the in vivo role of MSH7, we present data from this protein in Arabidopsis thaliana plants. First, we generated transgenic plants that express the β-glucuronidase (GUS) under the control of the MSH7 promoter. Histochemical staining of the transgenic plants indicated that MSH7 is preferentially expressed in proliferating tissues. Then, we identified msh7 T-DNA insertion mutants. Plants deficient in MSH7 show increased levels of UV-B-induced cyclobutane pyrimidine dimers (CPDs) relative to wild-type (WT) plants. Consistent with the patterns of MSH7 expression, we next analyzed the role of the protein during somatic and meiotic recombination. The frequency of somatic recombination between homologous or homeologous repeats (divergence level of 1.6%) was monitored using a previously described GUS recombination reporter assay. Disruption of MSH7 has no effect on the rates of somatic homologous or homeologous recombination under control conditions or after UV-B exposure. However, the rate of meiotic recombination between two genetically linked seed-specific fluorescent markers was 97% higher in msh7 than in WT plants. Taken together, these results suggest that MSH7 is involved in UV-B-induced DNA damage recognition and in controlling meiotic recombination.
Fil: Lario, Luciana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Botta, Pablo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
description The mismatch repair (MMR) system maintains genome integrity by correcting replication associated errors and inhibiting recombination between divergent DNA sequences. The basic features of the pathway have been highly conserved throughout evolution, although the nature and number of the proteins involved in this DNA repair system vary among organisms. Plants have an extra mismatch recognition protein, named MutS. The protein is a heterodimer of MSH2?MSH7. To further understand the in vivo role of MSH7, we present data from this protein in Arabidopsis thaliana plants. First, we generated transgenic plants that express the β-glucuronidase (GUS) under the control of the MSH7 promoter. Histochemical staining of the transgenic plants indicated that MSH7 is preferentially expressed in proliferating tissues. Then, we identified msh7 T-DNA insertion mutants. Plants deficient in MSH7 show increased levels of UV-B-induced cyclobutane pyrimidine dimers (CPDs) relative to wild-type (WT) plants. Consistent with the patterns of MSH7 expression, we next analyzed the role of the protein during somatic and meiotic recombination. The frequency of somatic recombination between homologous or homeologous repeats (divergence level of 1.6%) was monitored using a previously described GUS recombination reporter assay. Disruption of MSH7 has no effect on the rates of somatic homologous or homeologous recombination under control conditions or after UV-B exposure. However, the rate of meiotic recombination between two genetically linked seed-specific fluorescent markers was 97% higher in msh7 than in WT plants. Taken together, these results suggest that MSH7 is involved in UV-B-induced DNA damage recognition and in controlling meiotic recombination.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7855
Lario, Luciana Daniela; Botta, Pablo Eduardo; Casati, Paula; Spampinato, Claudia Patricia; Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination; Oxford University Press; Journal Of Experimental Botany; 66; 11; 12-2014; 3019-3026
0022-0957
url http://hdl.handle.net/11336/7855
identifier_str_mv Lario, Luciana Daniela; Botta, Pablo Eduardo; Casati, Paula; Spampinato, Claudia Patricia; Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination; Oxford University Press; Journal Of Experimental Botany; 66; 11; 12-2014; 3019-3026
0022-0957
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jxb.oxfordjournals.org/content/66/11/3019.abstract
info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/eru464
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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