Effectiveness of coproscopic concentration techniques

Autores
Taglioretti, Verónica; Sardella, Norma Haydee; Fugassa, Martín Horacio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to compare the effectiveness of the concentration techniques of flotation-centrifugation with zinc chloride (FZn) (δ = 1.45) with the spontaneous sedimentation (SSed) and the sucrose flotation-centrifugation (FSuc) (δ = 1.2) to recuperate gastrointestinal parasites from camelid fecal samples. The technique with more positive results for the detection of Nematodirus sp., Trichuris sp., strongyle-type eggs and Eimeria macusaniensis oocysts was the FZn. For Trichuris sp. and Eimeria macusaniensis, the higher coverglass counts were detected by FZn procedure. No significant differences were registered among centrifugation flotation techniques for Nematodirus spp. Coverglass count for strongyle-type eggs was significantly higher for FSuc than FZn (p = 0.0005) or SSed (p = 0.0005), being also significantly higher for FZn than for SSed (p = 0.008). FZn is a sensitive technique that allows the recovery of parasite elements with high density and it exerts low osmotic pressure avoiding parasite deformation.
Fil: Taglioretti, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sardella, Norma Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fugassa, Martín Horacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Materia
Concentration Techniques
Zinc Chloride
Parasite
Camelid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34883
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Effectiveness of coproscopic concentration techniquesTaglioretti, VerónicaSardella, Norma HaydeeFugassa, Martín HoracioConcentration TechniquesZinc ChlorideParasiteCamelidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of the present study was to compare the effectiveness of the concentration techniques of flotation-centrifugation with zinc chloride (FZn) (δ = 1.45) with the spontaneous sedimentation (SSed) and the sucrose flotation-centrifugation (FSuc) (δ = 1.2) to recuperate gastrointestinal parasites from camelid fecal samples. The technique with more positive results for the detection of Nematodirus sp., Trichuris sp., strongyle-type eggs and Eimeria macusaniensis oocysts was the FZn. For Trichuris sp. and Eimeria macusaniensis, the higher coverglass counts were detected by FZn procedure. No significant differences were registered among centrifugation flotation techniques for Nematodirus spp. Coverglass count for strongyle-type eggs was significantly higher for FSuc than FZn (p = 0.0005) or SSed (p = 0.0005), being also significantly higher for FZn than for SSed (p = 0.008). FZn is a sensitive technique that allows the recovery of parasite elements with high density and it exerts low osmotic pressure avoiding parasite deformation.Fil: Taglioretti, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Sardella, Norma Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fugassa, Martín Horacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaVersita2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34883Taglioretti, Verónica; Sardella, Norma Haydee; Fugassa, Martín Horacio; Effectiveness of coproscopic concentration techniques; Versita; Helminthologia; 51; 3; 9-2014; 210-2140440-6605CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2478/s11687-014-0231-xinfo:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/helm.2014.51.issue-3/s11687-014-0231-x/s11687-014-0231-x.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:58:22Zoai:ri.conicet.gov.ar:11336/34883instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:58:22.834CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effectiveness of coproscopic concentration techniques
title Effectiveness of coproscopic concentration techniques
spellingShingle Effectiveness of coproscopic concentration techniques
Taglioretti, Verónica
Concentration Techniques
Zinc Chloride
Parasite
Camelid
title_short Effectiveness of coproscopic concentration techniques
title_full Effectiveness of coproscopic concentration techniques
title_fullStr Effectiveness of coproscopic concentration techniques
title_full_unstemmed Effectiveness of coproscopic concentration techniques
title_sort Effectiveness of coproscopic concentration techniques
dc.creator.none.fl_str_mv Taglioretti, Verónica
Sardella, Norma Haydee
Fugassa, Martín Horacio
author Taglioretti, Verónica
author_facet Taglioretti, Verónica
Sardella, Norma Haydee
Fugassa, Martín Horacio
author_role author
author2 Sardella, Norma Haydee
Fugassa, Martín Horacio
author2_role author
author
dc.subject.none.fl_str_mv Concentration Techniques
Zinc Chloride
Parasite
Camelid
topic Concentration Techniques
Zinc Chloride
Parasite
Camelid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of the present study was to compare the effectiveness of the concentration techniques of flotation-centrifugation with zinc chloride (FZn) (δ = 1.45) with the spontaneous sedimentation (SSed) and the sucrose flotation-centrifugation (FSuc) (δ = 1.2) to recuperate gastrointestinal parasites from camelid fecal samples. The technique with more positive results for the detection of Nematodirus sp., Trichuris sp., strongyle-type eggs and Eimeria macusaniensis oocysts was the FZn. For Trichuris sp. and Eimeria macusaniensis, the higher coverglass counts were detected by FZn procedure. No significant differences were registered among centrifugation flotation techniques for Nematodirus spp. Coverglass count for strongyle-type eggs was significantly higher for FSuc than FZn (p = 0.0005) or SSed (p = 0.0005), being also significantly higher for FZn than for SSed (p = 0.008). FZn is a sensitive technique that allows the recovery of parasite elements with high density and it exerts low osmotic pressure avoiding parasite deformation.
Fil: Taglioretti, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sardella, Norma Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fugassa, Martín Horacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
description The aim of the present study was to compare the effectiveness of the concentration techniques of flotation-centrifugation with zinc chloride (FZn) (δ = 1.45) with the spontaneous sedimentation (SSed) and the sucrose flotation-centrifugation (FSuc) (δ = 1.2) to recuperate gastrointestinal parasites from camelid fecal samples. The technique with more positive results for the detection of Nematodirus sp., Trichuris sp., strongyle-type eggs and Eimeria macusaniensis oocysts was the FZn. For Trichuris sp. and Eimeria macusaniensis, the higher coverglass counts were detected by FZn procedure. No significant differences were registered among centrifugation flotation techniques for Nematodirus spp. Coverglass count for strongyle-type eggs was significantly higher for FSuc than FZn (p = 0.0005) or SSed (p = 0.0005), being also significantly higher for FZn than for SSed (p = 0.008). FZn is a sensitive technique that allows the recovery of parasite elements with high density and it exerts low osmotic pressure avoiding parasite deformation.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34883
Taglioretti, Verónica; Sardella, Norma Haydee; Fugassa, Martín Horacio; Effectiveness of coproscopic concentration techniques; Versita; Helminthologia; 51; 3; 9-2014; 210-214
0440-6605
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34883
identifier_str_mv Taglioretti, Verónica; Sardella, Norma Haydee; Fugassa, Martín Horacio; Effectiveness of coproscopic concentration techniques; Versita; Helminthologia; 51; 3; 9-2014; 210-214
0440-6605
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2478/s11687-014-0231-x
info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/helm.2014.51.issue-3/s11687-014-0231-x/s11687-014-0231-x.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Versita
publisher.none.fl_str_mv Versita
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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