Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri
- Autores
- Romero, Delfina Mercedes; Rios, Maria del Carmen; Juárez, Angela Beatriz
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a Chlorella kessleri tolerant strain by exposure to the herbicide. After 96h of exposure to increasing concentrations of the herbicide (0-70mgL-1) with alkylaryl polyglycol ether surfactant, growth was inhibited (EC50-96h 55.62mgL-1). Glyphosate increased protein and malondialdehyde content which was significantly higher than in the control at 50-70mgL-1. Superoxide dismutase and catalase activities and reduced glutathione levels increased in a concentration-dependant manner. Morphological studies showed increases in vacuolisation and in cell and sporangia sizes. The glyphosate formulation studied has a cytotoxic effect on C. kessleri through a mechanism that would involve the induction of oxidative stress. Upon glyphosate exposure, oxidative stress parameters such as SOD and CAT activities and MDA level could be more sensitive biomarkers than usually tested growth parameters in C. kessleri. © 2010 Elsevier Inc.
Fil: Romero, Delfina Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Enzimología, Estrés Oxidativo y Metabolismo; Argentina
Fil: Rios, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Enzimología, Estrés Oxidativo y Metabolismo; Argentina
Fil: Juárez, Angela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Enzimología, Estrés Oxidativo y Metabolismo; Argentina - Materia
-
Antioxidant Defences
Chlorella Kessleri
Glyphosate
Microalgae
Oxidative Stress - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/66456
Ver los metadatos del registro completo
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Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleriRomero, Delfina MercedesRios, Maria del CarmenJuárez, Angela BeatrizAntioxidant DefencesChlorella KessleriGlyphosateMicroalgaeOxidative Stresshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a Chlorella kessleri tolerant strain by exposure to the herbicide. After 96h of exposure to increasing concentrations of the herbicide (0-70mgL-1) with alkylaryl polyglycol ether surfactant, growth was inhibited (EC50-96h 55.62mgL-1). Glyphosate increased protein and malondialdehyde content which was significantly higher than in the control at 50-70mgL-1. Superoxide dismutase and catalase activities and reduced glutathione levels increased in a concentration-dependant manner. Morphological studies showed increases in vacuolisation and in cell and sporangia sizes. The glyphosate formulation studied has a cytotoxic effect on C. kessleri through a mechanism that would involve the induction of oxidative stress. Upon glyphosate exposure, oxidative stress parameters such as SOD and CAT activities and MDA level could be more sensitive biomarkers than usually tested growth parameters in C. kessleri. © 2010 Elsevier Inc.Fil: Romero, Delfina Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Enzimología, Estrés Oxidativo y Metabolismo; ArgentinaFil: Rios, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Enzimología, Estrés Oxidativo y Metabolismo; ArgentinaFil: Juárez, Angela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Enzimología, Estrés Oxidativo y Metabolismo; ArgentinaAcademic Press Inc Elsevier Science2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66456Romero, Delfina Mercedes; Rios, Maria del Carmen; Juárez, Angela Beatriz; Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 74; 4; 5-2011; 741-7470147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651310003398info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2010.10.034info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:18Zoai:ri.conicet.gov.ar:11336/66456instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:19.026CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
title |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
spellingShingle |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri Romero, Delfina Mercedes Antioxidant Defences Chlorella Kessleri Glyphosate Microalgae Oxidative Stress |
title_short |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
title_full |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
title_fullStr |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
title_full_unstemmed |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
title_sort |
Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri |
dc.creator.none.fl_str_mv |
Romero, Delfina Mercedes Rios, Maria del Carmen Juárez, Angela Beatriz |
author |
Romero, Delfina Mercedes |
author_facet |
Romero, Delfina Mercedes Rios, Maria del Carmen Juárez, Angela Beatriz |
author_role |
author |
author2 |
Rios, Maria del Carmen Juárez, Angela Beatriz |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Antioxidant Defences Chlorella Kessleri Glyphosate Microalgae Oxidative Stress |
topic |
Antioxidant Defences Chlorella Kessleri Glyphosate Microalgae Oxidative Stress |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
We studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a Chlorella kessleri tolerant strain by exposure to the herbicide. After 96h of exposure to increasing concentrations of the herbicide (0-70mgL-1) with alkylaryl polyglycol ether surfactant, growth was inhibited (EC50-96h 55.62mgL-1). Glyphosate increased protein and malondialdehyde content which was significantly higher than in the control at 50-70mgL-1. Superoxide dismutase and catalase activities and reduced glutathione levels increased in a concentration-dependant manner. Morphological studies showed increases in vacuolisation and in cell and sporangia sizes. The glyphosate formulation studied has a cytotoxic effect on C. kessleri through a mechanism that would involve the induction of oxidative stress. Upon glyphosate exposure, oxidative stress parameters such as SOD and CAT activities and MDA level could be more sensitive biomarkers than usually tested growth parameters in C. kessleri. © 2010 Elsevier Inc. Fil: Romero, Delfina Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Enzimología, Estrés Oxidativo y Metabolismo; Argentina Fil: Rios, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Enzimología, Estrés Oxidativo y Metabolismo; Argentina Fil: Juárez, Angela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Enzimología, Estrés Oxidativo y Metabolismo; Argentina |
description |
We studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a Chlorella kessleri tolerant strain by exposure to the herbicide. After 96h of exposure to increasing concentrations of the herbicide (0-70mgL-1) with alkylaryl polyglycol ether surfactant, growth was inhibited (EC50-96h 55.62mgL-1). Glyphosate increased protein and malondialdehyde content which was significantly higher than in the control at 50-70mgL-1. Superoxide dismutase and catalase activities and reduced glutathione levels increased in a concentration-dependant manner. Morphological studies showed increases in vacuolisation and in cell and sporangia sizes. The glyphosate formulation studied has a cytotoxic effect on C. kessleri through a mechanism that would involve the induction of oxidative stress. Upon glyphosate exposure, oxidative stress parameters such as SOD and CAT activities and MDA level could be more sensitive biomarkers than usually tested growth parameters in C. kessleri. © 2010 Elsevier Inc. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/66456 Romero, Delfina Mercedes; Rios, Maria del Carmen; Juárez, Angela Beatriz; Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 74; 4; 5-2011; 741-747 0147-6513 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/66456 |
identifier_str_mv |
Romero, Delfina Mercedes; Rios, Maria del Carmen; Juárez, Angela Beatriz; Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 74; 4; 5-2011; 741-747 0147-6513 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651310003398 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2010.10.034 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613333155577856 |
score |
13.070432 |