Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy
- Autores
- Passucci, Victoria; Thomas Chemin, Ophélie; Dib, Omar; Assaf, Antony Ali; Durand, Marie José; Dague, Etienne; Areco, María del Mar; Cécile, Formosa-dague
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Microalgae, such as Parachlorella kessleri, have significant potential for environmental remediation, especially in removing heavy metals like zinc from water. This study investigates how P. kessleri, isolated from a polluted river in Argentina, can remediate zinc. Using atomic force microscopy (AFM), the research examined the interactions between Zn particles and cells grown with different nitrogen sources—nitrate or ammonium. The results showed that cells grown with nitrate produced extracellular polymeric substances (EPS), while those grown with ammonium did not. Raman spectroscopy revealed distinct metabolic responses based on the nitrogen source, with nitrate-grown cells showing altered profiles after zinc exposure. Zinc exposure also changed the surface roughness and nanomechanical properties of the cells, particularly in those producing EPS. AFM force spectroscopy experiments then confirmed strong Zn binding to EPS in nitrate-grown cells, while interactions were weaker in ammonium-grown cells that lacked EPS. Overall, our results elucidate the critical role of EPS in Zn removal by P. kessleri cells and show that Zn remediation by P. kessleri is mediated by EPS adsorption. This study underscores the significance of regulating nitrogen sources to stimulate EPS production, offering insights that are essential for subsequent bioremediation applications.
Fil: Passucci, Victoria. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina
Fil: Thomas Chemin, Ophélie. Instituto Polytechnique de Toulouse; Francia
Fil: Dib, Omar. Universite de Nantes; Francia
Fil: Assaf, Antony Ali. Universite de Nantes; Francia
Fil: Durand, Marie José. Universite de Nantes; Francia
Fil: Dague, Etienne. Instituto Polytechnique de Toulouse; Francia
Fil: Areco, María del Mar. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina
Fil: Cécile, Formosa-dague. Instituto Polytechnique de Toulouse; Francia - Materia
-
Parachlorella kessleri
BIOREMEDIATION
HEAVY METAL
EXTRACELLULAR POLYMERIC SUBSTANCES
ATOMIC FORCE MICROSCOPY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/266909
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
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Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopyPassucci, VictoriaThomas Chemin, OphélieDib, OmarAssaf, Antony AliDurand, Marie JoséDague, EtienneAreco, María del MarCécile, Formosa-dagueParachlorella kessleriBIOREMEDIATIONHEAVY METALEXTRACELLULAR POLYMERIC SUBSTANCESATOMIC FORCE MICROSCOPYhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Microalgae, such as Parachlorella kessleri, have significant potential for environmental remediation, especially in removing heavy metals like zinc from water. This study investigates how P. kessleri, isolated from a polluted river in Argentina, can remediate zinc. Using atomic force microscopy (AFM), the research examined the interactions between Zn particles and cells grown with different nitrogen sources—nitrate or ammonium. The results showed that cells grown with nitrate produced extracellular polymeric substances (EPS), while those grown with ammonium did not. Raman spectroscopy revealed distinct metabolic responses based on the nitrogen source, with nitrate-grown cells showing altered profiles after zinc exposure. Zinc exposure also changed the surface roughness and nanomechanical properties of the cells, particularly in those producing EPS. AFM force spectroscopy experiments then confirmed strong Zn binding to EPS in nitrate-grown cells, while interactions were weaker in ammonium-grown cells that lacked EPS. Overall, our results elucidate the critical role of EPS in Zn removal by P. kessleri cells and show that Zn remediation by P. kessleri is mediated by EPS adsorption. This study underscores the significance of regulating nitrogen sources to stimulate EPS production, offering insights that are essential for subsequent bioremediation applications.Fil: Passucci, Victoria. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; ArgentinaFil: Thomas Chemin, Ophélie. Instituto Polytechnique de Toulouse; FranciaFil: Dib, Omar. Universite de Nantes; FranciaFil: Assaf, Antony Ali. Universite de Nantes; FranciaFil: Durand, Marie José. Universite de Nantes; FranciaFil: Dague, Etienne. Instituto Polytechnique de Toulouse; FranciaFil: Areco, María del Mar. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; ArgentinaFil: Cécile, Formosa-dague. Instituto Polytechnique de Toulouse; FranciaElsevier2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266909Passucci, Victoria; Thomas Chemin, Ophélie; Dib, Omar; Assaf, Antony Ali; Durand, Marie José; et al.; Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy; Elsevier; Environmental Pollution; 363; 10-2024; 1-290269-7491CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0269749124017998info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2024.125082info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:53Zoai:ri.conicet.gov.ar:11336/266909instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:54.15CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy |
title |
Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy |
spellingShingle |
Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy Passucci, Victoria Parachlorella kessleri BIOREMEDIATION HEAVY METAL EXTRACELLULAR POLYMERIC SUBSTANCES ATOMIC FORCE MICROSCOPY |
title_short |
Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy |
title_full |
Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy |
title_fullStr |
Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy |
title_full_unstemmed |
Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy |
title_sort |
Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy |
dc.creator.none.fl_str_mv |
Passucci, Victoria Thomas Chemin, Ophélie Dib, Omar Assaf, Antony Ali Durand, Marie José Dague, Etienne Areco, María del Mar Cécile, Formosa-dague |
author |
Passucci, Victoria |
author_facet |
Passucci, Victoria Thomas Chemin, Ophélie Dib, Omar Assaf, Antony Ali Durand, Marie José Dague, Etienne Areco, María del Mar Cécile, Formosa-dague |
author_role |
author |
author2 |
Thomas Chemin, Ophélie Dib, Omar Assaf, Antony Ali Durand, Marie José Dague, Etienne Areco, María del Mar Cécile, Formosa-dague |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Parachlorella kessleri BIOREMEDIATION HEAVY METAL EXTRACELLULAR POLYMERIC SUBSTANCES ATOMIC FORCE MICROSCOPY |
topic |
Parachlorella kessleri BIOREMEDIATION HEAVY METAL EXTRACELLULAR POLYMERIC SUBSTANCES ATOMIC FORCE MICROSCOPY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Microalgae, such as Parachlorella kessleri, have significant potential for environmental remediation, especially in removing heavy metals like zinc from water. This study investigates how P. kessleri, isolated from a polluted river in Argentina, can remediate zinc. Using atomic force microscopy (AFM), the research examined the interactions between Zn particles and cells grown with different nitrogen sources—nitrate or ammonium. The results showed that cells grown with nitrate produced extracellular polymeric substances (EPS), while those grown with ammonium did not. Raman spectroscopy revealed distinct metabolic responses based on the nitrogen source, with nitrate-grown cells showing altered profiles after zinc exposure. Zinc exposure also changed the surface roughness and nanomechanical properties of the cells, particularly in those producing EPS. AFM force spectroscopy experiments then confirmed strong Zn binding to EPS in nitrate-grown cells, while interactions were weaker in ammonium-grown cells that lacked EPS. Overall, our results elucidate the critical role of EPS in Zn removal by P. kessleri cells and show that Zn remediation by P. kessleri is mediated by EPS adsorption. This study underscores the significance of regulating nitrogen sources to stimulate EPS production, offering insights that are essential for subsequent bioremediation applications. Fil: Passucci, Victoria. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina Fil: Thomas Chemin, Ophélie. Instituto Polytechnique de Toulouse; Francia Fil: Dib, Omar. Universite de Nantes; Francia Fil: Assaf, Antony Ali. Universite de Nantes; Francia Fil: Durand, Marie José. Universite de Nantes; Francia Fil: Dague, Etienne. Instituto Polytechnique de Toulouse; Francia Fil: Areco, María del Mar. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina Fil: Cécile, Formosa-dague. Instituto Polytechnique de Toulouse; Francia |
description |
Microalgae, such as Parachlorella kessleri, have significant potential for environmental remediation, especially in removing heavy metals like zinc from water. This study investigates how P. kessleri, isolated from a polluted river in Argentina, can remediate zinc. Using atomic force microscopy (AFM), the research examined the interactions between Zn particles and cells grown with different nitrogen sources—nitrate or ammonium. The results showed that cells grown with nitrate produced extracellular polymeric substances (EPS), while those grown with ammonium did not. Raman spectroscopy revealed distinct metabolic responses based on the nitrogen source, with nitrate-grown cells showing altered profiles after zinc exposure. Zinc exposure also changed the surface roughness and nanomechanical properties of the cells, particularly in those producing EPS. AFM force spectroscopy experiments then confirmed strong Zn binding to EPS in nitrate-grown cells, while interactions were weaker in ammonium-grown cells that lacked EPS. Overall, our results elucidate the critical role of EPS in Zn removal by P. kessleri cells and show that Zn remediation by P. kessleri is mediated by EPS adsorption. This study underscores the significance of regulating nitrogen sources to stimulate EPS production, offering insights that are essential for subsequent bioremediation applications. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/266909 Passucci, Victoria; Thomas Chemin, Ophélie; Dib, Omar; Assaf, Antony Ali; Durand, Marie José; et al.; Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy; Elsevier; Environmental Pollution; 363; 10-2024; 1-29 0269-7491 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/266909 |
identifier_str_mv |
Passucci, Victoria; Thomas Chemin, Ophélie; Dib, Omar; Assaf, Antony Ali; Durand, Marie José; et al.; Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy; Elsevier; Environmental Pollution; 363; 10-2024; 1-29 0269-7491 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0269749124017998 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2024.125082 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614060991053824 |
score |
13.070432 |