Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy

Autores
Passucci, Victoria; Thomas Chemin, Ophélie; Dib, Omar; Assaf, Antony Ali; Durand, Marie José; Dague, Etienne; Areco, María del Mar; Cécile, Formosa-dague
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microalgae, such as Parachlorella kessleri, have significant potential for environmental remediation, especially in removing heavy metals like zinc from water. This study investigates how P. kessleri, isolated from a polluted river in Argentina, can remediate zinc. Using atomic force microscopy (AFM), the research examined the interactions between Zn particles and cells grown with different nitrogen sources—nitrate or ammonium. The results showed that cells grown with nitrate produced extracellular polymeric substances (EPS), while those grown with ammonium did not. Raman spectroscopy revealed distinct metabolic responses based on the nitrogen source, with nitrate-grown cells showing altered profiles after zinc exposure. Zinc exposure also changed the surface roughness and nanomechanical properties of the cells, particularly in those producing EPS. AFM force spectroscopy experiments then confirmed strong Zn binding to EPS in nitrate-grown cells, while interactions were weaker in ammonium-grown cells that lacked EPS. Overall, our results elucidate the critical role of EPS in Zn removal by P. kessleri cells and show that Zn remediation by P. kessleri is mediated by EPS adsorption. This study underscores the significance of regulating nitrogen sources to stimulate EPS production, offering insights that are essential for subsequent bioremediation applications.
Fil: Passucci, Victoria. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina
Fil: Thomas Chemin, Ophélie. Instituto Polytechnique de Toulouse; Francia
Fil: Dib, Omar. Universite de Nantes; Francia
Fil: Assaf, Antony Ali. Universite de Nantes; Francia
Fil: Durand, Marie José. Universite de Nantes; Francia
Fil: Dague, Etienne. Instituto Polytechnique de Toulouse; Francia
Fil: Areco, María del Mar. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina
Fil: Cécile, Formosa-dague. Instituto Polytechnique de Toulouse; Francia
Materia
Parachlorella kessleri
BIOREMEDIATION
HEAVY METAL
EXTRACELLULAR POLYMERIC SUBSTANCES
ATOMIC FORCE MICROSCOPY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266909
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/266909
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopyPassucci, VictoriaThomas Chemin, OphélieDib, OmarAssaf, Antony AliDurand, Marie JoséDague, EtienneAreco, María del MarCécile, Formosa-dagueParachlorella kessleriBIOREMEDIATIONHEAVY METALEXTRACELLULAR POLYMERIC SUBSTANCESATOMIC FORCE MICROSCOPYhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Microalgae, such as Parachlorella kessleri, have significant potential for environmental remediation, especially in removing heavy metals like zinc from water. This study investigates how P. kessleri, isolated from a polluted river in Argentina, can remediate zinc. Using atomic force microscopy (AFM), the research examined the interactions between Zn particles and cells grown with different nitrogen sources—nitrate or ammonium. The results showed that cells grown with nitrate produced extracellular polymeric substances (EPS), while those grown with ammonium did not. Raman spectroscopy revealed distinct metabolic responses based on the nitrogen source, with nitrate-grown cells showing altered profiles after zinc exposure. Zinc exposure also changed the surface roughness and nanomechanical properties of the cells, particularly in those producing EPS. AFM force spectroscopy experiments then confirmed strong Zn binding to EPS in nitrate-grown cells, while interactions were weaker in ammonium-grown cells that lacked EPS. Overall, our results elucidate the critical role of EPS in Zn removal by P. kessleri cells and show that Zn remediation by P. kessleri is mediated by EPS adsorption. This study underscores the significance of regulating nitrogen sources to stimulate EPS production, offering insights that are essential for subsequent bioremediation applications.Fil: Passucci, Victoria. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; ArgentinaFil: Thomas Chemin, Ophélie. Instituto Polytechnique de Toulouse; FranciaFil: Dib, Omar. Universite de Nantes; FranciaFil: Assaf, Antony Ali. Universite de Nantes; FranciaFil: Durand, Marie José. Universite de Nantes; FranciaFil: Dague, Etienne. Instituto Polytechnique de Toulouse; FranciaFil: Areco, María del Mar. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; ArgentinaFil: Cécile, Formosa-dague. Instituto Polytechnique de Toulouse; FranciaElsevier2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266909Passucci, Victoria; Thomas Chemin, Ophélie; Dib, Omar; Assaf, Antony Ali; Durand, Marie José; et al.; Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy; Elsevier; Environmental Pollution; 363; 10-2024; 1-290269-7491CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0269749124017998info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2024.125082info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:43:53Zoai:ri.conicet.gov.ar:11336/266909instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:43:53.469CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy
title Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy
spellingShingle Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy
Passucci, Victoria
Parachlorella kessleri
BIOREMEDIATION
HEAVY METAL
EXTRACELLULAR POLYMERIC SUBSTANCES
ATOMIC FORCE MICROSCOPY
title_short Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy
title_full Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy
title_fullStr Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy
title_full_unstemmed Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy
title_sort Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy
dc.creator.none.fl_str_mv Passucci, Victoria
Thomas Chemin, Ophélie
Dib, Omar
Assaf, Antony Ali
Durand, Marie José
Dague, Etienne
Areco, María del Mar
Cécile, Formosa-dague
author Passucci, Victoria
author_facet Passucci, Victoria
Thomas Chemin, Ophélie
Dib, Omar
Assaf, Antony Ali
Durand, Marie José
Dague, Etienne
Areco, María del Mar
Cécile, Formosa-dague
author_role author
author2 Thomas Chemin, Ophélie
Dib, Omar
Assaf, Antony Ali
Durand, Marie José
Dague, Etienne
Areco, María del Mar
Cécile, Formosa-dague
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Parachlorella kessleri
BIOREMEDIATION
HEAVY METAL
EXTRACELLULAR POLYMERIC SUBSTANCES
ATOMIC FORCE MICROSCOPY
topic Parachlorella kessleri
BIOREMEDIATION
HEAVY METAL
EXTRACELLULAR POLYMERIC SUBSTANCES
ATOMIC FORCE MICROSCOPY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Microalgae, such as Parachlorella kessleri, have significant potential for environmental remediation, especially in removing heavy metals like zinc from water. This study investigates how P. kessleri, isolated from a polluted river in Argentina, can remediate zinc. Using atomic force microscopy (AFM), the research examined the interactions between Zn particles and cells grown with different nitrogen sources—nitrate or ammonium. The results showed that cells grown with nitrate produced extracellular polymeric substances (EPS), while those grown with ammonium did not. Raman spectroscopy revealed distinct metabolic responses based on the nitrogen source, with nitrate-grown cells showing altered profiles after zinc exposure. Zinc exposure also changed the surface roughness and nanomechanical properties of the cells, particularly in those producing EPS. AFM force spectroscopy experiments then confirmed strong Zn binding to EPS in nitrate-grown cells, while interactions were weaker in ammonium-grown cells that lacked EPS. Overall, our results elucidate the critical role of EPS in Zn removal by P. kessleri cells and show that Zn remediation by P. kessleri is mediated by EPS adsorption. This study underscores the significance of regulating nitrogen sources to stimulate EPS production, offering insights that are essential for subsequent bioremediation applications.
Fil: Passucci, Victoria. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina
Fil: Thomas Chemin, Ophélie. Instituto Polytechnique de Toulouse; Francia
Fil: Dib, Omar. Universite de Nantes; Francia
Fil: Assaf, Antony Ali. Universite de Nantes; Francia
Fil: Durand, Marie José. Universite de Nantes; Francia
Fil: Dague, Etienne. Instituto Polytechnique de Toulouse; Francia
Fil: Areco, María del Mar. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina
Fil: Cécile, Formosa-dague. Instituto Polytechnique de Toulouse; Francia
description Microalgae, such as Parachlorella kessleri, have significant potential for environmental remediation, especially in removing heavy metals like zinc from water. This study investigates how P. kessleri, isolated from a polluted river in Argentina, can remediate zinc. Using atomic force microscopy (AFM), the research examined the interactions between Zn particles and cells grown with different nitrogen sources—nitrate or ammonium. The results showed that cells grown with nitrate produced extracellular polymeric substances (EPS), while those grown with ammonium did not. Raman spectroscopy revealed distinct metabolic responses based on the nitrogen source, with nitrate-grown cells showing altered profiles after zinc exposure. Zinc exposure also changed the surface roughness and nanomechanical properties of the cells, particularly in those producing EPS. AFM force spectroscopy experiments then confirmed strong Zn binding to EPS in nitrate-grown cells, while interactions were weaker in ammonium-grown cells that lacked EPS. Overall, our results elucidate the critical role of EPS in Zn removal by P. kessleri cells and show that Zn remediation by P. kessleri is mediated by EPS adsorption. This study underscores the significance of regulating nitrogen sources to stimulate EPS production, offering insights that are essential for subsequent bioremediation applications.
publishDate 2024
dc.date.none.fl_str_mv 2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266909
Passucci, Victoria; Thomas Chemin, Ophélie; Dib, Omar; Assaf, Antony Ali; Durand, Marie José; et al.; Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy; Elsevier; Environmental Pollution; 363; 10-2024; 1-29
0269-7491
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266909
identifier_str_mv Passucci, Victoria; Thomas Chemin, Ophélie; Dib, Omar; Assaf, Antony Ali; Durand, Marie José; et al.; Investigating the role of extracellular polymeric substances produced by Parachlorella kessleri in Zn(II) bioremediation using atomic force microscopy; Elsevier; Environmental Pollution; 363; 10-2024; 1-29
0269-7491
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0269749124017998
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2024.125082
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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