Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants

Autores
Pastor, Nicolás Alejandro; Rosas, Susana Beatriz; Luna, Maria Virginia; Rovera, Marisa
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of phosphate solubilizing plant growth-promoting microorganisms as inoculants assists in the hydrolysis of insoluble forms of phosphorus leading to increased plant growth. Pseudomonas putida PCI2 was evaluated for phosphatase activity and solubilization of AlPO4 and FePO4. The effect of different incubation temperatures, concentrations of NaCl and different pH on growth of PCI2 and P solubilization was studied. PCI2 proved to be positive for phosphatase activity, solubilized AlPO4 and hydrolyzed Ca3(PO4)2 even in medium with 5 % NaCl. In addition, PCI2 produced 45 % units of siderophores. The production of IAA by PCI2 was stimulated in vitro by the addition of different concentrations of L-tryptophan to the culture medium. Assays with tomato seedlings showed that the length of the root was reduced as the concentration of IAA increased. On the other hand, inoculation with PCI2 caused a clear growth-promoting effect on shoot growth in the presence of L-tryptophan. P. putida PCI2 is adapted to different environmental conditions and has potential to be developed and used as an inoculant for increasing the growth of tomato plants.
Fil: Pastor, Nicolás Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Rosas, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Luna, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Rovera, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Materia
Pseudomonas Putida Pci2
Phosphate Solubilization
Different Environmental Conditions
Indole-3-Acetic Acid
Tomato
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33950
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plantsPastor, Nicolás AlejandroRosas, Susana BeatrizLuna, Maria VirginiaRovera, MarisaPseudomonas Putida Pci2Phosphate SolubilizationDifferent Environmental ConditionsIndole-3-Acetic AcidTomatohttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The use of phosphate solubilizing plant growth-promoting microorganisms as inoculants assists in the hydrolysis of insoluble forms of phosphorus leading to increased plant growth. Pseudomonas putida PCI2 was evaluated for phosphatase activity and solubilization of AlPO4 and FePO4. The effect of different incubation temperatures, concentrations of NaCl and different pH on growth of PCI2 and P solubilization was studied. PCI2 proved to be positive for phosphatase activity, solubilized AlPO4 and hydrolyzed Ca3(PO4)2 even in medium with 5 % NaCl. In addition, PCI2 produced 45 % units of siderophores. The production of IAA by PCI2 was stimulated in vitro by the addition of different concentrations of L-tryptophan to the culture medium. Assays with tomato seedlings showed that the length of the root was reduced as the concentration of IAA increased. On the other hand, inoculation with PCI2 caused a clear growth-promoting effect on shoot growth in the presence of L-tryptophan. P. putida PCI2 is adapted to different environmental conditions and has potential to be developed and used as an inoculant for increasing the growth of tomato plants.Fil: Pastor, Nicolás Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Rosas, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Luna, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; ArgentinaFil: Rovera, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaSpringer2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33950Pastor, Nicolás Alejandro; Rosas, Susana Beatriz; Luna, Maria Virginia; Rovera, Marisa; Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants; Springer; Symbiosis; 62; 3; 3-2014; 157-1670334-5114CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s13199-014-0281-3info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs13199-014-0281-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:05Zoai:ri.conicet.gov.ar:11336/33950instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:05.762CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants
title Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants
spellingShingle Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants
Pastor, Nicolás Alejandro
Pseudomonas Putida Pci2
Phosphate Solubilization
Different Environmental Conditions
Indole-3-Acetic Acid
Tomato
title_short Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants
title_full Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants
title_fullStr Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants
title_full_unstemmed Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants
title_sort Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants
dc.creator.none.fl_str_mv Pastor, Nicolás Alejandro
Rosas, Susana Beatriz
Luna, Maria Virginia
Rovera, Marisa
author Pastor, Nicolás Alejandro
author_facet Pastor, Nicolás Alejandro
Rosas, Susana Beatriz
Luna, Maria Virginia
Rovera, Marisa
author_role author
author2 Rosas, Susana Beatriz
Luna, Maria Virginia
Rovera, Marisa
author2_role author
author
author
dc.subject.none.fl_str_mv Pseudomonas Putida Pci2
Phosphate Solubilization
Different Environmental Conditions
Indole-3-Acetic Acid
Tomato
topic Pseudomonas Putida Pci2
Phosphate Solubilization
Different Environmental Conditions
Indole-3-Acetic Acid
Tomato
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The use of phosphate solubilizing plant growth-promoting microorganisms as inoculants assists in the hydrolysis of insoluble forms of phosphorus leading to increased plant growth. Pseudomonas putida PCI2 was evaluated for phosphatase activity and solubilization of AlPO4 and FePO4. The effect of different incubation temperatures, concentrations of NaCl and different pH on growth of PCI2 and P solubilization was studied. PCI2 proved to be positive for phosphatase activity, solubilized AlPO4 and hydrolyzed Ca3(PO4)2 even in medium with 5 % NaCl. In addition, PCI2 produced 45 % units of siderophores. The production of IAA by PCI2 was stimulated in vitro by the addition of different concentrations of L-tryptophan to the culture medium. Assays with tomato seedlings showed that the length of the root was reduced as the concentration of IAA increased. On the other hand, inoculation with PCI2 caused a clear growth-promoting effect on shoot growth in the presence of L-tryptophan. P. putida PCI2 is adapted to different environmental conditions and has potential to be developed and used as an inoculant for increasing the growth of tomato plants.
Fil: Pastor, Nicolás Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Rosas, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Luna, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Rovera, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
description The use of phosphate solubilizing plant growth-promoting microorganisms as inoculants assists in the hydrolysis of insoluble forms of phosphorus leading to increased plant growth. Pseudomonas putida PCI2 was evaluated for phosphatase activity and solubilization of AlPO4 and FePO4. The effect of different incubation temperatures, concentrations of NaCl and different pH on growth of PCI2 and P solubilization was studied. PCI2 proved to be positive for phosphatase activity, solubilized AlPO4 and hydrolyzed Ca3(PO4)2 even in medium with 5 % NaCl. In addition, PCI2 produced 45 % units of siderophores. The production of IAA by PCI2 was stimulated in vitro by the addition of different concentrations of L-tryptophan to the culture medium. Assays with tomato seedlings showed that the length of the root was reduced as the concentration of IAA increased. On the other hand, inoculation with PCI2 caused a clear growth-promoting effect on shoot growth in the presence of L-tryptophan. P. putida PCI2 is adapted to different environmental conditions and has potential to be developed and used as an inoculant for increasing the growth of tomato plants.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33950
Pastor, Nicolás Alejandro; Rosas, Susana Beatriz; Luna, Maria Virginia; Rovera, Marisa; Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants; Springer; Symbiosis; 62; 3; 3-2014; 157-167
0334-5114
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33950
identifier_str_mv Pastor, Nicolás Alejandro; Rosas, Susana Beatriz; Luna, Maria Virginia; Rovera, Marisa; Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants; Springer; Symbiosis; 62; 3; 3-2014; 157-167
0334-5114
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs13199-014-0281-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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