Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633)

Autores
Liffourrena, Andres Sebastian; Lucchesi, Gloria Ines
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pseudomonas putida A (ATCC 12633) capable of degrading trimethylamine (TMA) was immobilized in calcium alginate. The TMA-degrading capacity of immobilized cells was compared to free cells in batch culture by changing temperature, pH, nutrient supplementation, and initial TMA concentration. Although immobilized cells showed good removal efficiencies in wider ranges of temperature (15 °C–35 °C) and pH (6.5–8.5) than free cells, the optimal conditions for TMA removal for both free and immobilized cells were 30 °C, in buffered medium (pH 7.5) without addition of nutrients. Immobilized cells degraded up 885 mg l−1 of TMA completely whereas the free cells degraded only 295 mg l−1. For all the TMA concentrations evaluated with immobilized cells (295–885 mg l−1), after 24 h, the degradation rate started to decline. This effect was attributed to the accumulation of intracellular TMA into the cells (8.3–15.3 mg l−1) which is sufficient to inhibit the first enzyme of the aerobic degradation of TMA by P. putida, the TMA dehydrogenase. The fact that the immobilized cells showed a high stability as regards their viability and degradability of high TMA concentrations revealed a good potential of this system for treating of TMA-contaminated site.
Fil: Liffourrena, Andres Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Lucchesi, Gloria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Materia
Trimethylamine
Pseudomonas Putida
Biodegradation
Alginate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33938
Acceder
id CONICETDig_bd3ce1e10f24a6a9bdf879f633a36131
oai_identifier_str oai:ri.conicet.gov.ar:11336/33938
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633)Liffourrena, Andres SebastianLucchesi, Gloria InesTrimethylaminePseudomonas PutidaBiodegradationAlginatehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Pseudomonas putida A (ATCC 12633) capable of degrading trimethylamine (TMA) was immobilized in calcium alginate. The TMA-degrading capacity of immobilized cells was compared to free cells in batch culture by changing temperature, pH, nutrient supplementation, and initial TMA concentration. Although immobilized cells showed good removal efficiencies in wider ranges of temperature (15 °C–35 °C) and pH (6.5–8.5) than free cells, the optimal conditions for TMA removal for both free and immobilized cells were 30 °C, in buffered medium (pH 7.5) without addition of nutrients. Immobilized cells degraded up 885 mg l−1 of TMA completely whereas the free cells degraded only 295 mg l−1. For all the TMA concentrations evaluated with immobilized cells (295–885 mg l−1), after 24 h, the degradation rate started to decline. This effect was attributed to the accumulation of intracellular TMA into the cells (8.3–15.3 mg l−1) which is sufficient to inhibit the first enzyme of the aerobic degradation of TMA by P. putida, the TMA dehydrogenase. The fact that the immobilized cells showed a high stability as regards their viability and degradability of high TMA concentrations revealed a good potential of this system for treating of TMA-contaminated site.Fil: Liffourrena, Andres Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Lucchesi, Gloria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaElsevier2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33938Liffourrena, Andres Sebastian; Lucchesi, Gloria Ines; Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633); Elsevier; International Biodeterioration and Biodegradation; 90; 3-2014; 88-920964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2014.02.008info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830514000535info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:20Zoai:ri.conicet.gov.ar:11336/33938instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:20.525CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633)
title Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633)
spellingShingle Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633)
Liffourrena, Andres Sebastian
Trimethylamine
Pseudomonas Putida
Biodegradation
Alginate
title_short Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633)
title_full Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633)
title_fullStr Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633)
title_full_unstemmed Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633)
title_sort Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633)
dc.creator.none.fl_str_mv Liffourrena, Andres Sebastian
Lucchesi, Gloria Ines
author Liffourrena, Andres Sebastian
author_facet Liffourrena, Andres Sebastian
Lucchesi, Gloria Ines
author_role author
author2 Lucchesi, Gloria Ines
author2_role author
dc.subject.none.fl_str_mv Trimethylamine
Pseudomonas Putida
Biodegradation
Alginate
topic Trimethylamine
Pseudomonas Putida
Biodegradation
Alginate
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Pseudomonas putida A (ATCC 12633) capable of degrading trimethylamine (TMA) was immobilized in calcium alginate. The TMA-degrading capacity of immobilized cells was compared to free cells in batch culture by changing temperature, pH, nutrient supplementation, and initial TMA concentration. Although immobilized cells showed good removal efficiencies in wider ranges of temperature (15 °C–35 °C) and pH (6.5–8.5) than free cells, the optimal conditions for TMA removal for both free and immobilized cells were 30 °C, in buffered medium (pH 7.5) without addition of nutrients. Immobilized cells degraded up 885 mg l−1 of TMA completely whereas the free cells degraded only 295 mg l−1. For all the TMA concentrations evaluated with immobilized cells (295–885 mg l−1), after 24 h, the degradation rate started to decline. This effect was attributed to the accumulation of intracellular TMA into the cells (8.3–15.3 mg l−1) which is sufficient to inhibit the first enzyme of the aerobic degradation of TMA by P. putida, the TMA dehydrogenase. The fact that the immobilized cells showed a high stability as regards their viability and degradability of high TMA concentrations revealed a good potential of this system for treating of TMA-contaminated site.
Fil: Liffourrena, Andres Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Lucchesi, Gloria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
description Pseudomonas putida A (ATCC 12633) capable of degrading trimethylamine (TMA) was immobilized in calcium alginate. The TMA-degrading capacity of immobilized cells was compared to free cells in batch culture by changing temperature, pH, nutrient supplementation, and initial TMA concentration. Although immobilized cells showed good removal efficiencies in wider ranges of temperature (15 °C–35 °C) and pH (6.5–8.5) than free cells, the optimal conditions for TMA removal for both free and immobilized cells were 30 °C, in buffered medium (pH 7.5) without addition of nutrients. Immobilized cells degraded up 885 mg l−1 of TMA completely whereas the free cells degraded only 295 mg l−1. For all the TMA concentrations evaluated with immobilized cells (295–885 mg l−1), after 24 h, the degradation rate started to decline. This effect was attributed to the accumulation of intracellular TMA into the cells (8.3–15.3 mg l−1) which is sufficient to inhibit the first enzyme of the aerobic degradation of TMA by P. putida, the TMA dehydrogenase. The fact that the immobilized cells showed a high stability as regards their viability and degradability of high TMA concentrations revealed a good potential of this system for treating of TMA-contaminated site.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33938
Liffourrena, Andres Sebastian; Lucchesi, Gloria Ines; Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633); Elsevier; International Biodeterioration and Biodegradation; 90; 3-2014; 88-92
0964-8305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33938
identifier_str_mv Liffourrena, Andres Sebastian; Lucchesi, Gloria Ines; Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633); Elsevier; International Biodeterioration and Biodegradation; 90; 3-2014; 88-92
0964-8305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2014.02.008
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830514000535
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268852165017600
score 13.13397

Publicaciones similares