Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida

Autores
Boeris, Paola Sabrina; Agustín, María del Rosario; Acevedo, Diego Fernando; Lucchesi, Gloria Ines
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Living and non-living biomass of Pseudomonas putida A (ATCC 12633) was used as biosorbent for the removing of Al3+ from aqueous solutions. The process was stable with time, efficient at pH 4.3 and between 15 °C and 42 °C. Two isotherms models were applied to describe the interaction between the biosorbent and Al3+. Non-living biomass of P. putida A (ATCC 12633) was found to be the most efficient at adsorbing Al3+ with a maximum sorption capacity of 0.55 mg Al3+/gr adsorbent and with 36 × 105 binding sites of Al3+/microorganisms. Infrared spectroscopy analysis shows that the biosorbent present some vibrational band of functional groups that change in presence of Al3+: hydroxyl, carboxyl and phosphate. Considering that Al3+ binds to the phosphate group of phosphatidylcholine, non-viable biomass of P. putida PB01 (mutant lacking phosphatidylcholine) was used. Aluminum adsorption of the parental strain was 30 times higher than values registered in P. putida PB01 (36 × 105 sites/microorganism vs 1.2 × 105 sites/microorganism, respectively). This result evidenced that the absence of phosphatidylcholine significantly affected the availability of the binding sites and consequently the efficiency of the biomass to adsorb Al3+.
Fil: Boeris, Paola Sabrina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Agustín, María del Rosario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Acevedo, Diego Fernando. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Lucchesi, Gloria Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
ALUMINUM
BIOSORPTION
PSEUDOMONAS PUTIDA
TOXIC METALS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/179997
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/179997
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putidaBoeris, Paola SabrinaAgustín, María del RosarioAcevedo, Diego FernandoLucchesi, Gloria InesALUMINUMBIOSORPTIONPSEUDOMONAS PUTIDATOXIC METALShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Living and non-living biomass of Pseudomonas putida A (ATCC 12633) was used as biosorbent for the removing of Al3+ from aqueous solutions. The process was stable with time, efficient at pH 4.3 and between 15 °C and 42 °C. Two isotherms models were applied to describe the interaction between the biosorbent and Al3+. Non-living biomass of P. putida A (ATCC 12633) was found to be the most efficient at adsorbing Al3+ with a maximum sorption capacity of 0.55 mg Al3+/gr adsorbent and with 36 × 105 binding sites of Al3+/microorganisms. Infrared spectroscopy analysis shows that the biosorbent present some vibrational band of functional groups that change in presence of Al3+: hydroxyl, carboxyl and phosphate. Considering that Al3+ binds to the phosphate group of phosphatidylcholine, non-viable biomass of P. putida PB01 (mutant lacking phosphatidylcholine) was used. Aluminum adsorption of the parental strain was 30 times higher than values registered in P. putida PB01 (36 × 105 sites/microorganism vs 1.2 × 105 sites/microorganism, respectively). This result evidenced that the absence of phosphatidylcholine significantly affected the availability of the binding sites and consequently the efficiency of the biomass to adsorb Al3+.Fil: Boeris, Paola Sabrina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Agustín, María del Rosario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Acevedo, Diego Fernando. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Lucchesi, Gloria Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaElsevier Science2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/179997Boeris, Paola Sabrina; Agustín, María del Rosario; Acevedo, Diego Fernando; Lucchesi, Gloria Ines; Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida; Elsevier Science; Journal of Biotechnology; 236; 7-2016; 57-630168-1656CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0168165616314365info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jbiotec.2016.07.026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:39:39Zoai:ri.conicet.gov.ar:11336/179997instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:39:39.388CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida
title Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida
spellingShingle Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida
Boeris, Paola Sabrina
ALUMINUM
BIOSORPTION
PSEUDOMONAS PUTIDA
TOXIC METALS
title_short Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida
title_full Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida
title_fullStr Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida
title_full_unstemmed Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida
title_sort Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida
dc.creator.none.fl_str_mv Boeris, Paola Sabrina
Agustín, María del Rosario
Acevedo, Diego Fernando
Lucchesi, Gloria Ines
author Boeris, Paola Sabrina
author_facet Boeris, Paola Sabrina
Agustín, María del Rosario
Acevedo, Diego Fernando
Lucchesi, Gloria Ines
author_role author
author2 Agustín, María del Rosario
Acevedo, Diego Fernando
Lucchesi, Gloria Ines
author2_role author
author
author
dc.subject.none.fl_str_mv ALUMINUM
BIOSORPTION
PSEUDOMONAS PUTIDA
TOXIC METALS
topic ALUMINUM
BIOSORPTION
PSEUDOMONAS PUTIDA
TOXIC METALS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Living and non-living biomass of Pseudomonas putida A (ATCC 12633) was used as biosorbent for the removing of Al3+ from aqueous solutions. The process was stable with time, efficient at pH 4.3 and between 15 °C and 42 °C. Two isotherms models were applied to describe the interaction between the biosorbent and Al3+. Non-living biomass of P. putida A (ATCC 12633) was found to be the most efficient at adsorbing Al3+ with a maximum sorption capacity of 0.55 mg Al3+/gr adsorbent and with 36 × 105 binding sites of Al3+/microorganisms. Infrared spectroscopy analysis shows that the biosorbent present some vibrational band of functional groups that change in presence of Al3+: hydroxyl, carboxyl and phosphate. Considering that Al3+ binds to the phosphate group of phosphatidylcholine, non-viable biomass of P. putida PB01 (mutant lacking phosphatidylcholine) was used. Aluminum adsorption of the parental strain was 30 times higher than values registered in P. putida PB01 (36 × 105 sites/microorganism vs 1.2 × 105 sites/microorganism, respectively). This result evidenced that the absence of phosphatidylcholine significantly affected the availability of the binding sites and consequently the efficiency of the biomass to adsorb Al3+.
Fil: Boeris, Paola Sabrina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Agustín, María del Rosario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Acevedo, Diego Fernando. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Lucchesi, Gloria Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description Living and non-living biomass of Pseudomonas putida A (ATCC 12633) was used as biosorbent for the removing of Al3+ from aqueous solutions. The process was stable with time, efficient at pH 4.3 and between 15 °C and 42 °C. Two isotherms models were applied to describe the interaction between the biosorbent and Al3+. Non-living biomass of P. putida A (ATCC 12633) was found to be the most efficient at adsorbing Al3+ with a maximum sorption capacity of 0.55 mg Al3+/gr adsorbent and with 36 × 105 binding sites of Al3+/microorganisms. Infrared spectroscopy analysis shows that the biosorbent present some vibrational band of functional groups that change in presence of Al3+: hydroxyl, carboxyl and phosphate. Considering that Al3+ binds to the phosphate group of phosphatidylcholine, non-viable biomass of P. putida PB01 (mutant lacking phosphatidylcholine) was used. Aluminum adsorption of the parental strain was 30 times higher than values registered in P. putida PB01 (36 × 105 sites/microorganism vs 1.2 × 105 sites/microorganism, respectively). This result evidenced that the absence of phosphatidylcholine significantly affected the availability of the binding sites and consequently the efficiency of the biomass to adsorb Al3+.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/179997
Boeris, Paola Sabrina; Agustín, María del Rosario; Acevedo, Diego Fernando; Lucchesi, Gloria Ines; Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida; Elsevier Science; Journal of Biotechnology; 236; 7-2016; 57-63
0168-1656
CONICET Digital
CONICET
url http://hdl.handle.net/11336/179997
identifier_str_mv Boeris, Paola Sabrina; Agustín, María del Rosario; Acevedo, Diego Fernando; Lucchesi, Gloria Ines; Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida; Elsevier Science; Journal of Biotechnology; 236; 7-2016; 57-63
0168-1656
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0168165616314365
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jbiotec.2016.07.026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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