Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages

Autores
Suárez, Viviana Beatriz; Zago, Miriam; Quiberoni, Andrea del Lujan; Carminati, Domenico; Giraffa, Giorgio; Reinheimer, Jorge Alberto
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: Frequency of lysogeny in Lactobacillus delbrueckii strains (from commercial and natural starters) and preliminary characterization of temperate bacteriophages isolated from them. Methods and Results: Induction of strains (a total of 16) was made using mitomycin C (MC) (0.5 μg ml-1). For 37% of the MC-treated supernatants, it was possible to detect phage particles or presence of killing activity, but only two active bacteriophages were isolated. The two temperate phages isolated were prolate-headed phages which belonged to group c of Lact. delbrueckii bacteriophages classification. Different DNA restriction patterns were obtained for each phage, while the structural protein profiles and packaging sites were identical. Distinctive one-step growth curves were exhibited by each phage. An influence of calcium ions was observed for their lysis in broth but not on the adsorption levels. Conclusions: Our study showed that lysogeny is also present in Lact. delbrueckii strains, including commercial strains. Significance and Impact of the Study: Commercial strains could be lysogenic and this fact has a great practical importance since they could contribute to the dissemination of active-phage particles in industrial environments.
Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Zago, Miriam. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; Italia
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Carminati, Domenico. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; Italia
Fil: Giraffa, Giorgio. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; Italia
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Materia
BACTERIOPHAGE CLASSIFICATION
DAIRY PROCESSES
LACTOBACILLUS DELBRUECKII
LYSOGENY
PHAGE ADSORPTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/117183
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/117183
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophagesSuárez, Viviana BeatrizZago, MiriamQuiberoni, Andrea del LujanCarminati, DomenicoGiraffa, GiorgioReinheimer, Jorge AlbertoBACTERIOPHAGE CLASSIFICATIONDAIRY PROCESSESLACTOBACILLUS DELBRUECKIILYSOGENYPHAGE ADSORPTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: Frequency of lysogeny in Lactobacillus delbrueckii strains (from commercial and natural starters) and preliminary characterization of temperate bacteriophages isolated from them. Methods and Results: Induction of strains (a total of 16) was made using mitomycin C (MC) (0.5 μg ml-1). For 37% of the MC-treated supernatants, it was possible to detect phage particles or presence of killing activity, but only two active bacteriophages were isolated. The two temperate phages isolated were prolate-headed phages which belonged to group c of Lact. delbrueckii bacteriophages classification. Different DNA restriction patterns were obtained for each phage, while the structural protein profiles and packaging sites were identical. Distinctive one-step growth curves were exhibited by each phage. An influence of calcium ions was observed for their lysis in broth but not on the adsorption levels. Conclusions: Our study showed that lysogeny is also present in Lact. delbrueckii strains, including commercial strains. Significance and Impact of the Study: Commercial strains could be lysogenic and this fact has a great practical importance since they could contribute to the dissemination of active-phage particles in industrial environments.Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Zago, Miriam. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; ItaliaFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Carminati, Domenico. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; ItaliaFil: Giraffa, Giorgio. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; ItaliaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaWiley Blackwell Publishing, Inc2008-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/117183Suárez, Viviana Beatriz; Zago, Miriam; Quiberoni, Andrea del Lujan; Carminati, Domenico; Giraffa, Giorgio; et al.; Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 105; 5; 11-2008; 1402-14111364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2008.03876.xinfo:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2672.2008.03876.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:59:45Zoai:ri.conicet.gov.ar:11336/117183instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:59:46.063CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages
title Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages
spellingShingle Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages
Suárez, Viviana Beatriz
BACTERIOPHAGE CLASSIFICATION
DAIRY PROCESSES
LACTOBACILLUS DELBRUECKII
LYSOGENY
PHAGE ADSORPTION
title_short Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages
title_full Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages
title_fullStr Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages
title_full_unstemmed Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages
title_sort Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages
dc.creator.none.fl_str_mv Suárez, Viviana Beatriz
Zago, Miriam
Quiberoni, Andrea del Lujan
Carminati, Domenico
Giraffa, Giorgio
Reinheimer, Jorge Alberto
author Suárez, Viviana Beatriz
author_facet Suárez, Viviana Beatriz
Zago, Miriam
Quiberoni, Andrea del Lujan
Carminati, Domenico
Giraffa, Giorgio
Reinheimer, Jorge Alberto
author_role author
author2 Zago, Miriam
Quiberoni, Andrea del Lujan
Carminati, Domenico
Giraffa, Giorgio
Reinheimer, Jorge Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIOPHAGE CLASSIFICATION
DAIRY PROCESSES
LACTOBACILLUS DELBRUECKII
LYSOGENY
PHAGE ADSORPTION
topic BACTERIOPHAGE CLASSIFICATION
DAIRY PROCESSES
LACTOBACILLUS DELBRUECKII
LYSOGENY
PHAGE ADSORPTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aims: Frequency of lysogeny in Lactobacillus delbrueckii strains (from commercial and natural starters) and preliminary characterization of temperate bacteriophages isolated from them. Methods and Results: Induction of strains (a total of 16) was made using mitomycin C (MC) (0.5 μg ml-1). For 37% of the MC-treated supernatants, it was possible to detect phage particles or presence of killing activity, but only two active bacteriophages were isolated. The two temperate phages isolated were prolate-headed phages which belonged to group c of Lact. delbrueckii bacteriophages classification. Different DNA restriction patterns were obtained for each phage, while the structural protein profiles and packaging sites were identical. Distinctive one-step growth curves were exhibited by each phage. An influence of calcium ions was observed for their lysis in broth but not on the adsorption levels. Conclusions: Our study showed that lysogeny is also present in Lact. delbrueckii strains, including commercial strains. Significance and Impact of the Study: Commercial strains could be lysogenic and this fact has a great practical importance since they could contribute to the dissemination of active-phage particles in industrial environments.
Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Zago, Miriam. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; Italia
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Carminati, Domenico. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; Italia
Fil: Giraffa, Giorgio. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; Italia
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
description Aims: Frequency of lysogeny in Lactobacillus delbrueckii strains (from commercial and natural starters) and preliminary characterization of temperate bacteriophages isolated from them. Methods and Results: Induction of strains (a total of 16) was made using mitomycin C (MC) (0.5 μg ml-1). For 37% of the MC-treated supernatants, it was possible to detect phage particles or presence of killing activity, but only two active bacteriophages were isolated. The two temperate phages isolated were prolate-headed phages which belonged to group c of Lact. delbrueckii bacteriophages classification. Different DNA restriction patterns were obtained for each phage, while the structural protein profiles and packaging sites were identical. Distinctive one-step growth curves were exhibited by each phage. An influence of calcium ions was observed for their lysis in broth but not on the adsorption levels. Conclusions: Our study showed that lysogeny is also present in Lact. delbrueckii strains, including commercial strains. Significance and Impact of the Study: Commercial strains could be lysogenic and this fact has a great practical importance since they could contribute to the dissemination of active-phage particles in industrial environments.
publishDate 2008
dc.date.none.fl_str_mv 2008-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/117183
Suárez, Viviana Beatriz; Zago, Miriam; Quiberoni, Andrea del Lujan; Carminati, Domenico; Giraffa, Giorgio; et al.; Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 105; 5; 11-2008; 1402-1411
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/117183
identifier_str_mv Suárez, Viviana Beatriz; Zago, Miriam; Quiberoni, Andrea del Lujan; Carminati, Domenico; Giraffa, Giorgio; et al.; Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 105; 5; 11-2008; 1402-1411
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2008.03876.x
info:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2672.2008.03876.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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