Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages
- Autores
- Suárez, Viviana Beatriz; Zago, Miriam; Quiberoni, Andrea del Lujan; Carminati, Domenico; Giraffa, Giorgio; Reinheimer, Jorge Alberto
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Aims: Frequency of lysogeny in Lactobacillus delbrueckii strains (from commercial and natural starters) and preliminary characterization of temperate bacteriophages isolated from them. Methods and Results: Induction of strains (a total of 16) was made using mitomycin C (MC) (0.5 μg ml-1). For 37% of the MC-treated supernatants, it was possible to detect phage particles or presence of killing activity, but only two active bacteriophages were isolated. The two temperate phages isolated were prolate-headed phages which belonged to group c of Lact. delbrueckii bacteriophages classification. Different DNA restriction patterns were obtained for each phage, while the structural protein profiles and packaging sites were identical. Distinctive one-step growth curves were exhibited by each phage. An influence of calcium ions was observed for their lysis in broth but not on the adsorption levels. Conclusions: Our study showed that lysogeny is also present in Lact. delbrueckii strains, including commercial strains. Significance and Impact of the Study: Commercial strains could be lysogenic and this fact has a great practical importance since they could contribute to the dissemination of active-phage particles in industrial environments.
Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Zago, Miriam. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; Italia
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Carminati, Domenico. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; Italia
Fil: Giraffa, Giorgio. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; Italia
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina - Materia
-
BACTERIOPHAGE CLASSIFICATION
DAIRY PROCESSES
LACTOBACILLUS DELBRUECKII
LYSOGENY
PHAGE ADSORPTION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/117183
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Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophagesSuárez, Viviana BeatrizZago, MiriamQuiberoni, Andrea del LujanCarminati, DomenicoGiraffa, GiorgioReinheimer, Jorge AlbertoBACTERIOPHAGE CLASSIFICATIONDAIRY PROCESSESLACTOBACILLUS DELBRUECKIILYSOGENYPHAGE ADSORPTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: Frequency of lysogeny in Lactobacillus delbrueckii strains (from commercial and natural starters) and preliminary characterization of temperate bacteriophages isolated from them. Methods and Results: Induction of strains (a total of 16) was made using mitomycin C (MC) (0.5 μg ml-1). For 37% of the MC-treated supernatants, it was possible to detect phage particles or presence of killing activity, but only two active bacteriophages were isolated. The two temperate phages isolated were prolate-headed phages which belonged to group c of Lact. delbrueckii bacteriophages classification. Different DNA restriction patterns were obtained for each phage, while the structural protein profiles and packaging sites were identical. Distinctive one-step growth curves were exhibited by each phage. An influence of calcium ions was observed for their lysis in broth but not on the adsorption levels. Conclusions: Our study showed that lysogeny is also present in Lact. delbrueckii strains, including commercial strains. Significance and Impact of the Study: Commercial strains could be lysogenic and this fact has a great practical importance since they could contribute to the dissemination of active-phage particles in industrial environments.Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Zago, Miriam. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; ItaliaFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Carminati, Domenico. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; ItaliaFil: Giraffa, Giorgio. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; ItaliaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaWiley Blackwell Publishing, Inc2008-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/117183Suárez, Viviana Beatriz; Zago, Miriam; Quiberoni, Andrea del Lujan; Carminati, Domenico; Giraffa, Giorgio; et al.; Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 105; 5; 11-2008; 1402-14111364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2008.03876.xinfo:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2672.2008.03876.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:12Zoai:ri.conicet.gov.ar:11336/117183instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:12.929CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages |
title |
Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages |
spellingShingle |
Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages Suárez, Viviana Beatriz BACTERIOPHAGE CLASSIFICATION DAIRY PROCESSES LACTOBACILLUS DELBRUECKII LYSOGENY PHAGE ADSORPTION |
title_short |
Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages |
title_full |
Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages |
title_fullStr |
Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages |
title_full_unstemmed |
Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages |
title_sort |
Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages |
dc.creator.none.fl_str_mv |
Suárez, Viviana Beatriz Zago, Miriam Quiberoni, Andrea del Lujan Carminati, Domenico Giraffa, Giorgio Reinheimer, Jorge Alberto |
author |
Suárez, Viviana Beatriz |
author_facet |
Suárez, Viviana Beatriz Zago, Miriam Quiberoni, Andrea del Lujan Carminati, Domenico Giraffa, Giorgio Reinheimer, Jorge Alberto |
author_role |
author |
author2 |
Zago, Miriam Quiberoni, Andrea del Lujan Carminati, Domenico Giraffa, Giorgio Reinheimer, Jorge Alberto |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
BACTERIOPHAGE CLASSIFICATION DAIRY PROCESSES LACTOBACILLUS DELBRUECKII LYSOGENY PHAGE ADSORPTION |
topic |
BACTERIOPHAGE CLASSIFICATION DAIRY PROCESSES LACTOBACILLUS DELBRUECKII LYSOGENY PHAGE ADSORPTION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Aims: Frequency of lysogeny in Lactobacillus delbrueckii strains (from commercial and natural starters) and preliminary characterization of temperate bacteriophages isolated from them. Methods and Results: Induction of strains (a total of 16) was made using mitomycin C (MC) (0.5 μg ml-1). For 37% of the MC-treated supernatants, it was possible to detect phage particles or presence of killing activity, but only two active bacteriophages were isolated. The two temperate phages isolated were prolate-headed phages which belonged to group c of Lact. delbrueckii bacteriophages classification. Different DNA restriction patterns were obtained for each phage, while the structural protein profiles and packaging sites were identical. Distinctive one-step growth curves were exhibited by each phage. An influence of calcium ions was observed for their lysis in broth but not on the adsorption levels. Conclusions: Our study showed that lysogeny is also present in Lact. delbrueckii strains, including commercial strains. Significance and Impact of the Study: Commercial strains could be lysogenic and this fact has a great practical importance since they could contribute to the dissemination of active-phage particles in industrial environments. Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Zago, Miriam. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; Italia Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Carminati, Domenico. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; Italia Fil: Giraffa, Giorgio. Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie, Settore di Ricerca “Lattiero‐Caseario; Italia Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina |
description |
Aims: Frequency of lysogeny in Lactobacillus delbrueckii strains (from commercial and natural starters) and preliminary characterization of temperate bacteriophages isolated from them. Methods and Results: Induction of strains (a total of 16) was made using mitomycin C (MC) (0.5 μg ml-1). For 37% of the MC-treated supernatants, it was possible to detect phage particles or presence of killing activity, but only two active bacteriophages were isolated. The two temperate phages isolated were prolate-headed phages which belonged to group c of Lact. delbrueckii bacteriophages classification. Different DNA restriction patterns were obtained for each phage, while the structural protein profiles and packaging sites were identical. Distinctive one-step growth curves were exhibited by each phage. An influence of calcium ions was observed for their lysis in broth but not on the adsorption levels. Conclusions: Our study showed that lysogeny is also present in Lact. delbrueckii strains, including commercial strains. Significance and Impact of the Study: Commercial strains could be lysogenic and this fact has a great practical importance since they could contribute to the dissemination of active-phage particles in industrial environments. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/117183 Suárez, Viviana Beatriz; Zago, Miriam; Quiberoni, Andrea del Lujan; Carminati, Domenico; Giraffa, Giorgio; et al.; Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 105; 5; 11-2008; 1402-1411 1364-5072 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/117183 |
identifier_str_mv |
Suárez, Viviana Beatriz; Zago, Miriam; Quiberoni, Andrea del Lujan; Carminati, Domenico; Giraffa, Giorgio; et al.; Lysogeny in Lactobacillus delbrueckii strains and characterization of two new temperate prolate-headed bacteriophages; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 105; 5; 11-2008; 1402-1411 1364-5072 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2008.03876.x info:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2672.2008.03876.x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |