Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activity

Autores
Chequer Charan, Daniela Maria; Huang, Wenqing; Gomez Casati, Maria Eugenia; Wedemeyer, Carolina; Elgoyhen, Ana Belen; Hua, Yunfeng; Di Guilmi, Mariano Nicolás
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The auditory system in many mammals is immature at birth but fine-tuned in adults. Spontaneous neural activity in the inner ear was proven to play a critical role in guiding this process. This is shaped by an efferent pathway that descends from the brainstem (the medial olivocochlear system, MOC) which transiently contacts the inner hair cells. In the knock-in mouse model with enhanced MOC activity (L9′T), altered functionality of auditory brainstem responses (Boero et al., 2019) and synaptic dysfunction of calyx of Held (CH) in the medial nucleus of the trapezoid body (MNTB) (Di Guilmi et al., 2019) have been observed at different developmental stages. Furthermore, our unpublished results of intracellular dye injection experiment revealed a delayed innervation refinement in L9'T MNTB principal cells during development. In this work, we set out a comprehensive structural investigation of the CH-type synapses from different tonotopic regions at P22-25 by means of large-scale 3D electron microscopy. We observed the presence of MNTB cells co-innervated by multiple CHs in both WT and L9’T mice with a slightly higher percentage of this phenotype in the L9’T (5/19 cells, 26%) than in the WT (4/22 cells, 18%). This was found mainly in the lateral MNTB region in the L9’T mice (Lat: 30%, 6/20 cells vs. Med: 26%, 5/19 cells). These results suggest a long-lasting impact of enhanced MOC innervation on the structural refinement of central auditory synapses persisting beyond hearing onset.
Fil: Chequer Charan, Daniela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Huang, Wenqing. Shanghai Institute of Precision Medicine; China
Fil: Gomez Casati, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Hua, Yunfeng. Shanghai Institute of Precision Medicine; China
Fil: Di Guilmi, Mariano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXXVI Reunion Anual de la Sociedad Argentina de Investigación en Neurociencias: SAN2021
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
SISTEMA EFERENTE OLIVOCOCLEAR
MNTB
MICROSCOPÍA ELECTRÓNICA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/203293
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/203293
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activityChequer Charan, Daniela MariaHuang, WenqingGomez Casati, Maria EugeniaWedemeyer, CarolinaElgoyhen, Ana BelenHua, YunfengDi Guilmi, Mariano NicolásSISTEMA EFERENTE OLIVOCOCLEARMNTBMICROSCOPÍA ELECTRÓNICAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The auditory system in many mammals is immature at birth but fine-tuned in adults. Spontaneous neural activity in the inner ear was proven to play a critical role in guiding this process. This is shaped by an efferent pathway that descends from the brainstem (the medial olivocochlear system, MOC) which transiently contacts the inner hair cells. In the knock-in mouse model with enhanced MOC activity (L9′T), altered functionality of auditory brainstem responses (Boero et al., 2019) and synaptic dysfunction of calyx of Held (CH) in the medial nucleus of the trapezoid body (MNTB) (Di Guilmi et al., 2019) have been observed at different developmental stages. Furthermore, our unpublished results of intracellular dye injection experiment revealed a delayed innervation refinement in L9'T MNTB principal cells during development. In this work, we set out a comprehensive structural investigation of the CH-type synapses from different tonotopic regions at P22-25 by means of large-scale 3D electron microscopy. We observed the presence of MNTB cells co-innervated by multiple CHs in both WT and L9’T mice with a slightly higher percentage of this phenotype in the L9’T (5/19 cells, 26%) than in the WT (4/22 cells, 18%). This was found mainly in the lateral MNTB region in the L9’T mice (Lat: 30%, 6/20 cells vs. Med: 26%, 5/19 cells). These results suggest a long-lasting impact of enhanced MOC innervation on the structural refinement of central auditory synapses persisting beyond hearing onset.Fil: Chequer Charan, Daniela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Huang, Wenqing. Shanghai Institute of Precision Medicine; ChinaFil: Gomez Casati, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; ArgentinaFil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Hua, Yunfeng. Shanghai Institute of Precision Medicine; ChinaFil: Di Guilmi, Mariano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaXXXVI Reunion Anual de la Sociedad Argentina de Investigación en Neurociencias: SAN2021Ciudad Autónoma de Buenos AiresArgentinaSociedad Argentina de Investigación en NeurocienciasSociedad Argentina de Investigación en Neurociencias2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/203293Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activity; XXXVI Reunion Anual de la Sociedad Argentina de Investigación en Neurociencias: SAN2021; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 74-74CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://csan2021.saneurociencias.org.ar/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:10Zoai:ri.conicet.gov.ar:11336/203293instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:10.951CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activity
title Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activity
spellingShingle Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activity
Chequer Charan, Daniela Maria
SISTEMA EFERENTE OLIVOCOCLEAR
MNTB
MICROSCOPÍA ELECTRÓNICA
title_short Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activity
title_full Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activity
title_fullStr Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activity
title_full_unstemmed Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activity
title_sort Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activity
dc.creator.none.fl_str_mv Chequer Charan, Daniela Maria
Huang, Wenqing
Gomez Casati, Maria Eugenia
Wedemeyer, Carolina
Elgoyhen, Ana Belen
Hua, Yunfeng
Di Guilmi, Mariano Nicolás
author Chequer Charan, Daniela Maria
author_facet Chequer Charan, Daniela Maria
Huang, Wenqing
Gomez Casati, Maria Eugenia
Wedemeyer, Carolina
Elgoyhen, Ana Belen
Hua, Yunfeng
Di Guilmi, Mariano Nicolás
author_role author
author2 Huang, Wenqing
Gomez Casati, Maria Eugenia
Wedemeyer, Carolina
Elgoyhen, Ana Belen
Hua, Yunfeng
Di Guilmi, Mariano Nicolás
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv SISTEMA EFERENTE OLIVOCOCLEAR
MNTB
MICROSCOPÍA ELECTRÓNICA
topic SISTEMA EFERENTE OLIVOCOCLEAR
MNTB
MICROSCOPÍA ELECTRÓNICA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The auditory system in many mammals is immature at birth but fine-tuned in adults. Spontaneous neural activity in the inner ear was proven to play a critical role in guiding this process. This is shaped by an efferent pathway that descends from the brainstem (the medial olivocochlear system, MOC) which transiently contacts the inner hair cells. In the knock-in mouse model with enhanced MOC activity (L9′T), altered functionality of auditory brainstem responses (Boero et al., 2019) and synaptic dysfunction of calyx of Held (CH) in the medial nucleus of the trapezoid body (MNTB) (Di Guilmi et al., 2019) have been observed at different developmental stages. Furthermore, our unpublished results of intracellular dye injection experiment revealed a delayed innervation refinement in L9'T MNTB principal cells during development. In this work, we set out a comprehensive structural investigation of the CH-type synapses from different tonotopic regions at P22-25 by means of large-scale 3D electron microscopy. We observed the presence of MNTB cells co-innervated by multiple CHs in both WT and L9’T mice with a slightly higher percentage of this phenotype in the L9’T (5/19 cells, 26%) than in the WT (4/22 cells, 18%). This was found mainly in the lateral MNTB region in the L9’T mice (Lat: 30%, 6/20 cells vs. Med: 26%, 5/19 cells). These results suggest a long-lasting impact of enhanced MOC innervation on the structural refinement of central auditory synapses persisting beyond hearing onset.
Fil: Chequer Charan, Daniela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Huang, Wenqing. Shanghai Institute of Precision Medicine; China
Fil: Gomez Casati, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Hua, Yunfeng. Shanghai Institute of Precision Medicine; China
Fil: Di Guilmi, Mariano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXXVI Reunion Anual de la Sociedad Argentina de Investigación en Neurociencias: SAN2021
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación en Neurociencias
description The auditory system in many mammals is immature at birth but fine-tuned in adults. Spontaneous neural activity in the inner ear was proven to play a critical role in guiding this process. This is shaped by an efferent pathway that descends from the brainstem (the medial olivocochlear system, MOC) which transiently contacts the inner hair cells. In the knock-in mouse model with enhanced MOC activity (L9′T), altered functionality of auditory brainstem responses (Boero et al., 2019) and synaptic dysfunction of calyx of Held (CH) in the medial nucleus of the trapezoid body (MNTB) (Di Guilmi et al., 2019) have been observed at different developmental stages. Furthermore, our unpublished results of intracellular dye injection experiment revealed a delayed innervation refinement in L9'T MNTB principal cells during development. In this work, we set out a comprehensive structural investigation of the CH-type synapses from different tonotopic regions at P22-25 by means of large-scale 3D electron microscopy. We observed the presence of MNTB cells co-innervated by multiple CHs in both WT and L9’T mice with a slightly higher percentage of this phenotype in the L9’T (5/19 cells, 26%) than in the WT (4/22 cells, 18%). This was found mainly in the lateral MNTB region in the L9’T mice (Lat: 30%, 6/20 cells vs. Med: 26%, 5/19 cells). These results suggest a long-lasting impact of enhanced MOC innervation on the structural refinement of central auditory synapses persisting beyond hearing onset.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/203293
Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activity; XXXVI Reunion Anual de la Sociedad Argentina de Investigación en Neurociencias: SAN2021; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 74-74
CONICET Digital
CONICET
url http://hdl.handle.net/11336/203293
identifier_str_mv Morphological alterations of a central auditory synapse in a mouse model with enhanced medial olivocochlear efferent activity; XXXVI Reunion Anual de la Sociedad Argentina de Investigación en Neurociencias: SAN2021; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 74-74
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://csan2021.saneurociencias.org.ar/
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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