Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem

Autores
Chequer Charan, Daniela Maria; Hua, Yunfeng; Wang, Haoyu; Huang, Wenqing; Wang, Fangfang; Elgoyhen, Ana Belen; Boergens, Kevin M.; Di Guilmi, Mariano Nicolás
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The medial nucleus of the trapezoid body (MNTB) is an integral component of the auditory brainstem circuitry involved in sound localization. The giant presynaptic nerve terminal with multiple active zones, the calyx of Held (CH), is a hallmark of this nucleus, which mediates fast and synchronized glutamatergic synaptic transmission. To delineate how these synaptic structures adapt to reduced auditory afferents due to aging, we acquired and reconstructed circuitry-level volumes of mouse MNTB at different ages (3 weeks, 6, 18, and 24 months) using serial block-face electron microscopy. We used C57BL/6J, the most widely inbred mouse strain used for transgenic lines, which displays a type of age-related hearing loss. We found that MNTB neurons reduce in density with age. Surprisingly we observed an average of approximately 10% of poly-innervated MNTB neurons along the mouse lifespan, with prevalence in the low frequency region. Moreover, a tonotopy-dependent heterogeneity in CH morphology was observed in young but not in older mice. In conclusion, our data support the notion that age-related hearing impairments can be in part a direct consequence of several structural alterations and circuit remodeling in the brainstem.
Fil: Chequer Charan, Daniela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Hua, Yunfeng. Shanghai Institute Of Precision Medicine; China
Fil: Wang, Haoyu. Shanghai Institute Of Precision Medicine; China
Fil: Huang, Wenqing. Shanghai Institute Of Precision Medicine; China
Fil: Wang, Fangfang. Shanghai Institute Of Precision Medicine; China
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Boergens, Kevin M.. University of Illinois; Estados Unidos
Fil: Di Guilmi, Mariano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
MNTB
Aging
Serial block electron microscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201416
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstemChequer Charan, Daniela MariaHua, YunfengWang, HaoyuHuang, WenqingWang, FangfangElgoyhen, Ana BelenBoergens, Kevin M.Di Guilmi, Mariano NicolásMNTBAgingSerial block electron microscopyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The medial nucleus of the trapezoid body (MNTB) is an integral component of the auditory brainstem circuitry involved in sound localization. The giant presynaptic nerve terminal with multiple active zones, the calyx of Held (CH), is a hallmark of this nucleus, which mediates fast and synchronized glutamatergic synaptic transmission. To delineate how these synaptic structures adapt to reduced auditory afferents due to aging, we acquired and reconstructed circuitry-level volumes of mouse MNTB at different ages (3 weeks, 6, 18, and 24 months) using serial block-face electron microscopy. We used C57BL/6J, the most widely inbred mouse strain used for transgenic lines, which displays a type of age-related hearing loss. We found that MNTB neurons reduce in density with age. Surprisingly we observed an average of approximately 10% of poly-innervated MNTB neurons along the mouse lifespan, with prevalence in the low frequency region. Moreover, a tonotopy-dependent heterogeneity in CH morphology was observed in young but not in older mice. In conclusion, our data support the notion that age-related hearing impairments can be in part a direct consequence of several structural alterations and circuit remodeling in the brainstem.Fil: Chequer Charan, Daniela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Hua, Yunfeng. Shanghai Institute Of Precision Medicine; ChinaFil: Wang, Haoyu. Shanghai Institute Of Precision Medicine; ChinaFil: Huang, Wenqing. Shanghai Institute Of Precision Medicine; ChinaFil: Wang, Fangfang. Shanghai Institute Of Precision Medicine; ChinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Boergens, Kevin M.. University of Illinois; Estados UnidosFil: Di Guilmi, Mariano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFrontiers Media2022-12-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201416Chequer Charan, Daniela Maria; Hua, Yunfeng; Wang, Haoyu; Huang, Wenqing; Wang, Fangfang; et al.; Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem; Frontiers Media; Frontiers in Cellular Neuroscience; 16; 15-12-2022; 1-161662-51021662-5102CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fncel.2022.1070438/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fncel.2022.1070438info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:45Zoai:ri.conicet.gov.ar:11336/201416instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:46.195CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem
title Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem
spellingShingle Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem
Chequer Charan, Daniela Maria
MNTB
Aging
Serial block electron microscopy
title_short Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem
title_full Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem
title_fullStr Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem
title_full_unstemmed Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem
title_sort Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem
dc.creator.none.fl_str_mv Chequer Charan, Daniela Maria
Hua, Yunfeng
Wang, Haoyu
Huang, Wenqing
Wang, Fangfang
Elgoyhen, Ana Belen
Boergens, Kevin M.
Di Guilmi, Mariano Nicolás
author Chequer Charan, Daniela Maria
author_facet Chequer Charan, Daniela Maria
Hua, Yunfeng
Wang, Haoyu
Huang, Wenqing
Wang, Fangfang
Elgoyhen, Ana Belen
Boergens, Kevin M.
Di Guilmi, Mariano Nicolás
author_role author
author2 Hua, Yunfeng
Wang, Haoyu
Huang, Wenqing
Wang, Fangfang
Elgoyhen, Ana Belen
Boergens, Kevin M.
Di Guilmi, Mariano Nicolás
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MNTB
Aging
Serial block electron microscopy
topic MNTB
Aging
Serial block electron microscopy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The medial nucleus of the trapezoid body (MNTB) is an integral component of the auditory brainstem circuitry involved in sound localization. The giant presynaptic nerve terminal with multiple active zones, the calyx of Held (CH), is a hallmark of this nucleus, which mediates fast and synchronized glutamatergic synaptic transmission. To delineate how these synaptic structures adapt to reduced auditory afferents due to aging, we acquired and reconstructed circuitry-level volumes of mouse MNTB at different ages (3 weeks, 6, 18, and 24 months) using serial block-face electron microscopy. We used C57BL/6J, the most widely inbred mouse strain used for transgenic lines, which displays a type of age-related hearing loss. We found that MNTB neurons reduce in density with age. Surprisingly we observed an average of approximately 10% of poly-innervated MNTB neurons along the mouse lifespan, with prevalence in the low frequency region. Moreover, a tonotopy-dependent heterogeneity in CH morphology was observed in young but not in older mice. In conclusion, our data support the notion that age-related hearing impairments can be in part a direct consequence of several structural alterations and circuit remodeling in the brainstem.
Fil: Chequer Charan, Daniela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Hua, Yunfeng. Shanghai Institute Of Precision Medicine; China
Fil: Wang, Haoyu. Shanghai Institute Of Precision Medicine; China
Fil: Huang, Wenqing. Shanghai Institute Of Precision Medicine; China
Fil: Wang, Fangfang. Shanghai Institute Of Precision Medicine; China
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Boergens, Kevin M.. University of Illinois; Estados Unidos
Fil: Di Guilmi, Mariano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description The medial nucleus of the trapezoid body (MNTB) is an integral component of the auditory brainstem circuitry involved in sound localization. The giant presynaptic nerve terminal with multiple active zones, the calyx of Held (CH), is a hallmark of this nucleus, which mediates fast and synchronized glutamatergic synaptic transmission. To delineate how these synaptic structures adapt to reduced auditory afferents due to aging, we acquired and reconstructed circuitry-level volumes of mouse MNTB at different ages (3 weeks, 6, 18, and 24 months) using serial block-face electron microscopy. We used C57BL/6J, the most widely inbred mouse strain used for transgenic lines, which displays a type of age-related hearing loss. We found that MNTB neurons reduce in density with age. Surprisingly we observed an average of approximately 10% of poly-innervated MNTB neurons along the mouse lifespan, with prevalence in the low frequency region. Moreover, a tonotopy-dependent heterogeneity in CH morphology was observed in young but not in older mice. In conclusion, our data support the notion that age-related hearing impairments can be in part a direct consequence of several structural alterations and circuit remodeling in the brainstem.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201416
Chequer Charan, Daniela Maria; Hua, Yunfeng; Wang, Haoyu; Huang, Wenqing; Wang, Fangfang; et al.; Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem; Frontiers Media; Frontiers in Cellular Neuroscience; 16; 15-12-2022; 1-16
1662-5102
1662-5102
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201416
identifier_str_mv Chequer Charan, Daniela Maria; Hua, Yunfeng; Wang, Haoyu; Huang, Wenqing; Wang, Fangfang; et al.; Volume electron microscopy reveals age-related circuit remodeling in the auditory brainstem; Frontiers Media; Frontiers in Cellular Neuroscience; 16; 15-12-2022; 1-16
1662-5102
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fncel.2022.1070438/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fncel.2022.1070438
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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