P-selectin promotes neutrophil extracellular trap formation in mice

Autores
Etulain, Julia; Martinod, Kimberly; Wong, Siu Ling; Cifuni, Stephen M.; Schattner, Mirta Ana; Wagner, Denisa D.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neutrophil extracellular traps(NETs)canbereleased in thevasculature. Inaddition totrapping microbes, they promote inflammatory and thrombotic diseases. Considering that P-selectin induces prothrombotic and proinflammatory signaling, we studied the role of this selectin in NETformation.NETformation(NETosis)wasinducedbythrombin-activated platelets rosetting with neutrophils and was inhibited by anti-P-selectin aptamer or anti-P-selectin glycoprotein ligand-1 (PSGL-1) inhibitory antibody but was not induced by platelets from P-selectin2/2 mice. Moreover, NETosis was also promoted by P-selectinimmunoglobulin fusion protein butnotby control immunoglobulin.We isolatedneutrophils frommice engineered tooverproduce soluble P-selectin (P-selectinDCT/DCT mice). Although the levels of circulating DNA and nucleosomes (indicative of spontaneous NETosis) were normal in these mice, basal neutrophil histone citrullination and presence of P-selectin on circulating neutrophils were elevated. NET formationafter stimulationwithplatelet activatingfactor, ionomycin,orphorbol 12-myristate 13-acetatewassignificantlyenhanced, indicating that the P-selectinDCT/DCT neutrophils were primed for NETosis. In summary, P-selectin, cellular or soluble, through binding to PSGL-1, promotes NETosis, suggesting that this pathway is a potential therapeutic target for NET-related diseases.
Fil: Etulain, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Boston Children’s Hospital; Estados Unidos
Fil: Martinod, Kimberly. Boston Children’s Hospital; Estados Unidos. Harvard Medical School; Estados Unidos
Fil: Wong, Siu Ling. Boston Children’s Hospital; Estados Unidos. Harvard Medical School; Estados Unidos
Fil: Cifuni, Stephen M.. Boston Children’s Hospital; Estados Unidos
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Wagner, Denisa D.. Harvard Medical School; Estados Unidos. Boston Children’s Hospital; Estados Unidos
Materia
Inflammation
Nets
Platelets
P-Selectin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85010
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/85010
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling P-selectin promotes neutrophil extracellular trap formation in miceEtulain, JuliaMartinod, KimberlyWong, Siu LingCifuni, Stephen M.Schattner, Mirta AnaWagner, Denisa D.InflammationNetsPlateletsP-Selectinhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Neutrophil extracellular traps(NETs)canbereleased in thevasculature. Inaddition totrapping microbes, they promote inflammatory and thrombotic diseases. Considering that P-selectin induces prothrombotic and proinflammatory signaling, we studied the role of this selectin in NETformation.NETformation(NETosis)wasinducedbythrombin-activated platelets rosetting with neutrophils and was inhibited by anti-P-selectin aptamer or anti-P-selectin glycoprotein ligand-1 (PSGL-1) inhibitory antibody but was not induced by platelets from P-selectin2/2 mice. Moreover, NETosis was also promoted by P-selectinimmunoglobulin fusion protein butnotby control immunoglobulin.We isolatedneutrophils frommice engineered tooverproduce soluble P-selectin (P-selectinDCT/DCT mice). Although the levels of circulating DNA and nucleosomes (indicative of spontaneous NETosis) were normal in these mice, basal neutrophil histone citrullination and presence of P-selectin on circulating neutrophils were elevated. NET formationafter stimulationwithplatelet activatingfactor, ionomycin,orphorbol 12-myristate 13-acetatewassignificantlyenhanced, indicating that the P-selectinDCT/DCT neutrophils were primed for NETosis. In summary, P-selectin, cellular or soluble, through binding to PSGL-1, promotes NETosis, suggesting that this pathway is a potential therapeutic target for NET-related diseases.Fil: Etulain, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Boston Children’s Hospital; Estados UnidosFil: Martinod, Kimberly. Boston Children’s Hospital; Estados Unidos. Harvard Medical School; Estados UnidosFil: Wong, Siu Ling. Boston Children’s Hospital; Estados Unidos. Harvard Medical School; Estados UnidosFil: Cifuni, Stephen M.. Boston Children’s Hospital; Estados UnidosFil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Wagner, Denisa D.. Harvard Medical School; Estados Unidos. Boston Children’s Hospital; Estados UnidosAmerican Society of Hematology2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85010Etulain, Julia; Martinod, Kimberly; Wong, Siu Ling; Cifuni, Stephen M.; Schattner, Mirta Ana; et al.; P-selectin promotes neutrophil extracellular trap formation in mice; American Society of Hematology; Blood; 126; 2; 7-2015; 242-2460006-4971CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/www.bloodjournal.org/content/126/2/242info:eu-repo/semantics/altIdentifier/doi/10.1182/blood-2015-01-624023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:48Zoai:ri.conicet.gov.ar:11336/85010instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:49.11CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv P-selectin promotes neutrophil extracellular trap formation in mice
title P-selectin promotes neutrophil extracellular trap formation in mice
spellingShingle P-selectin promotes neutrophil extracellular trap formation in mice
Etulain, Julia
Inflammation
Nets
Platelets
P-Selectin
title_short P-selectin promotes neutrophil extracellular trap formation in mice
title_full P-selectin promotes neutrophil extracellular trap formation in mice
title_fullStr P-selectin promotes neutrophil extracellular trap formation in mice
title_full_unstemmed P-selectin promotes neutrophil extracellular trap formation in mice
title_sort P-selectin promotes neutrophil extracellular trap formation in mice
dc.creator.none.fl_str_mv Etulain, Julia
Martinod, Kimberly
Wong, Siu Ling
Cifuni, Stephen M.
Schattner, Mirta Ana
Wagner, Denisa D.
author Etulain, Julia
author_facet Etulain, Julia
Martinod, Kimberly
Wong, Siu Ling
Cifuni, Stephen M.
Schattner, Mirta Ana
Wagner, Denisa D.
author_role author
author2 Martinod, Kimberly
Wong, Siu Ling
Cifuni, Stephen M.
Schattner, Mirta Ana
Wagner, Denisa D.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Inflammation
Nets
Platelets
P-Selectin
topic Inflammation
Nets
Platelets
P-Selectin
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Neutrophil extracellular traps(NETs)canbereleased in thevasculature. Inaddition totrapping microbes, they promote inflammatory and thrombotic diseases. Considering that P-selectin induces prothrombotic and proinflammatory signaling, we studied the role of this selectin in NETformation.NETformation(NETosis)wasinducedbythrombin-activated platelets rosetting with neutrophils and was inhibited by anti-P-selectin aptamer or anti-P-selectin glycoprotein ligand-1 (PSGL-1) inhibitory antibody but was not induced by platelets from P-selectin2/2 mice. Moreover, NETosis was also promoted by P-selectinimmunoglobulin fusion protein butnotby control immunoglobulin.We isolatedneutrophils frommice engineered tooverproduce soluble P-selectin (P-selectinDCT/DCT mice). Although the levels of circulating DNA and nucleosomes (indicative of spontaneous NETosis) were normal in these mice, basal neutrophil histone citrullination and presence of P-selectin on circulating neutrophils were elevated. NET formationafter stimulationwithplatelet activatingfactor, ionomycin,orphorbol 12-myristate 13-acetatewassignificantlyenhanced, indicating that the P-selectinDCT/DCT neutrophils were primed for NETosis. In summary, P-selectin, cellular or soluble, through binding to PSGL-1, promotes NETosis, suggesting that this pathway is a potential therapeutic target for NET-related diseases.
Fil: Etulain, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Boston Children’s Hospital; Estados Unidos
Fil: Martinod, Kimberly. Boston Children’s Hospital; Estados Unidos. Harvard Medical School; Estados Unidos
Fil: Wong, Siu Ling. Boston Children’s Hospital; Estados Unidos. Harvard Medical School; Estados Unidos
Fil: Cifuni, Stephen M.. Boston Children’s Hospital; Estados Unidos
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Wagner, Denisa D.. Harvard Medical School; Estados Unidos. Boston Children’s Hospital; Estados Unidos
description Neutrophil extracellular traps(NETs)canbereleased in thevasculature. Inaddition totrapping microbes, they promote inflammatory and thrombotic diseases. Considering that P-selectin induces prothrombotic and proinflammatory signaling, we studied the role of this selectin in NETformation.NETformation(NETosis)wasinducedbythrombin-activated platelets rosetting with neutrophils and was inhibited by anti-P-selectin aptamer or anti-P-selectin glycoprotein ligand-1 (PSGL-1) inhibitory antibody but was not induced by platelets from P-selectin2/2 mice. Moreover, NETosis was also promoted by P-selectinimmunoglobulin fusion protein butnotby control immunoglobulin.We isolatedneutrophils frommice engineered tooverproduce soluble P-selectin (P-selectinDCT/DCT mice). Although the levels of circulating DNA and nucleosomes (indicative of spontaneous NETosis) were normal in these mice, basal neutrophil histone citrullination and presence of P-selectin on circulating neutrophils were elevated. NET formationafter stimulationwithplatelet activatingfactor, ionomycin,orphorbol 12-myristate 13-acetatewassignificantlyenhanced, indicating that the P-selectinDCT/DCT neutrophils were primed for NETosis. In summary, P-selectin, cellular or soluble, through binding to PSGL-1, promotes NETosis, suggesting that this pathway is a potential therapeutic target for NET-related diseases.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85010
Etulain, Julia; Martinod, Kimberly; Wong, Siu Ling; Cifuni, Stephen M.; Schattner, Mirta Ana; et al.; P-selectin promotes neutrophil extracellular trap formation in mice; American Society of Hematology; Blood; 126; 2; 7-2015; 242-246
0006-4971
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85010
identifier_str_mv Etulain, Julia; Martinod, Kimberly; Wong, Siu Ling; Cifuni, Stephen M.; Schattner, Mirta Ana; et al.; P-selectin promotes neutrophil extracellular trap formation in mice; American Society of Hematology; Blood; 126; 2; 7-2015; 242-246
0006-4971
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/www.bloodjournal.org/content/126/2/242
info:eu-repo/semantics/altIdentifier/doi/10.1182/blood-2015-01-624023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Hematology
publisher.none.fl_str_mv American Society of Hematology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269308425601024
score 13.13397

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