Nucleosomes and neutrophil extracellular traps in septic and burn patients

Autores
Kaufman, Tomás; Magosevich, Débora; Moreno, María Carolina; Guzman, María Alejandra; D'Atri, Lina Paola; Carestia, Agostina; Fandiño, María Eugenia; Fondevila, Carlos; Schattner, Mirta Ana
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
NETosis is a host defense mechanism associated with inflammation and tissue damage. Experimental models show that platelets and von Willebrand factor (VWF) are key elements for intravascular NETosis. We determined NETosis in septic and burn patients at 1 and 4days post-admission (dpa). Nucleosomes were elevated in patients. In septics, they correlated with Human Neutrophil Elastase (HNE)-DNA complexes and SOFA score at 1dpa, and were associated with mortality. Patient´s neutrophils had spontaneous NETosis and were unresponsive to stimulation. Although platelet P-selectin and TNF-α were increased in both groups, higher platelet TLR4 expression, VWF levels and IL-6 were found in septics at 1dpa. Neither platelet activation markers nor cytokines correlated with nucleosomes or HNE-DNA. Nucleosomes could be indicators of organ damage and predictors of mortality in septic but not in burn patients. Platelet activation, VWF and cytokines do not appear to be key mediators of NETosis in these patient groups.
Fil: Kaufman, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Magosevich, Débora. Clínica Sagrado Corazón; Argentina
Fil: Moreno, María Carolina. Clínica Bazterrica; Argentina
Fil: Guzman, María Alejandra. Gobiernos de la Ciudad de Buenos Aires. Hospital de Quemados Dr. Arturo Umberto Illia; Argentina
Fil: D'Atri, Lina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Carestia, Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Fandiño, María Eugenia. Clínica Sagrado Corazón; Argentina
Fil: Fondevila, Carlos. Clínica Bazterrica; Argentina
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Materia
NEUTROPHILS
NETS
SEPSIS
INFLAMMATION
PLATELETS
BURN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41343
Acceder
id CONICETDig_4d83b56938f94348a269b208fd1c0334
oai_identifier_str oai:ri.conicet.gov.ar:11336/41343
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nucleosomes and neutrophil extracellular traps in septic and burn patientsKaufman, TomásMagosevich, DéboraMoreno, María CarolinaGuzman, María AlejandraD'Atri, Lina PaolaCarestia, AgostinaFandiño, María EugeniaFondevila, CarlosSchattner, Mirta AnaNEUTROPHILSNETSSEPSISINFLAMMATIONPLATELETSBURNhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3NETosis is a host defense mechanism associated with inflammation and tissue damage. Experimental models show that platelets and von Willebrand factor (VWF) are key elements for intravascular NETosis. We determined NETosis in septic and burn patients at 1 and 4days post-admission (dpa). Nucleosomes were elevated in patients. In septics, they correlated with Human Neutrophil Elastase (HNE)-DNA complexes and SOFA score at 1dpa, and were associated with mortality. Patient´s neutrophils had spontaneous NETosis and were unresponsive to stimulation. Although platelet P-selectin and TNF-α were increased in both groups, higher platelet TLR4 expression, VWF levels and IL-6 were found in septics at 1dpa. Neither platelet activation markers nor cytokines correlated with nucleosomes or HNE-DNA. Nucleosomes could be indicators of organ damage and predictors of mortality in septic but not in burn patients. Platelet activation, VWF and cytokines do not appear to be key mediators of NETosis in these patient groups.Fil: Kaufman, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Magosevich, Débora. Clínica Sagrado Corazón; ArgentinaFil: Moreno, María Carolina. Clínica Bazterrica; ArgentinaFil: Guzman, María Alejandra. Gobiernos de la Ciudad de Buenos Aires. Hospital de Quemados Dr. Arturo Umberto Illia; ArgentinaFil: D'Atri, Lina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Carestia, Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Fandiño, María Eugenia. Clínica Sagrado Corazón; ArgentinaFil: Fondevila, Carlos. Clínica Bazterrica; ArgentinaFil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaAcademic Press Inc Elsevier Science2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41343Kaufman, Tomás; Magosevich, Débora; Moreno, María Carolina; Guzman, María Alejandra; D'Atri, Lina Paola; et al.; Nucleosomes and neutrophil extracellular traps in septic and burn patients; Academic Press Inc Elsevier Science; Clinical Immunology; 183; 10-2017; 254-2621521-6616CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S152166161730339Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.clim.2017.08.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:14Zoai:ri.conicet.gov.ar:11336/41343instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:14.652CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nucleosomes and neutrophil extracellular traps in septic and burn patients
title Nucleosomes and neutrophil extracellular traps in septic and burn patients
spellingShingle Nucleosomes and neutrophil extracellular traps in septic and burn patients
Kaufman, Tomás
NEUTROPHILS
NETS
SEPSIS
INFLAMMATION
PLATELETS
BURN
title_short Nucleosomes and neutrophil extracellular traps in septic and burn patients
title_full Nucleosomes and neutrophil extracellular traps in septic and burn patients
title_fullStr Nucleosomes and neutrophil extracellular traps in septic and burn patients
title_full_unstemmed Nucleosomes and neutrophil extracellular traps in septic and burn patients
title_sort Nucleosomes and neutrophil extracellular traps in septic and burn patients
dc.creator.none.fl_str_mv Kaufman, Tomás
Magosevich, Débora
Moreno, María Carolina
Guzman, María Alejandra
D'Atri, Lina Paola
Carestia, Agostina
Fandiño, María Eugenia
Fondevila, Carlos
Schattner, Mirta Ana
author Kaufman, Tomás
author_facet Kaufman, Tomás
Magosevich, Débora
Moreno, María Carolina
Guzman, María Alejandra
D'Atri, Lina Paola
Carestia, Agostina
Fandiño, María Eugenia
Fondevila, Carlos
Schattner, Mirta Ana
author_role author
author2 Magosevich, Débora
Moreno, María Carolina
Guzman, María Alejandra
D'Atri, Lina Paola
Carestia, Agostina
Fandiño, María Eugenia
Fondevila, Carlos
Schattner, Mirta Ana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NEUTROPHILS
NETS
SEPSIS
INFLAMMATION
PLATELETS
BURN
topic NEUTROPHILS
NETS
SEPSIS
INFLAMMATION
PLATELETS
BURN
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv NETosis is a host defense mechanism associated with inflammation and tissue damage. Experimental models show that platelets and von Willebrand factor (VWF) are key elements for intravascular NETosis. We determined NETosis in septic and burn patients at 1 and 4days post-admission (dpa). Nucleosomes were elevated in patients. In septics, they correlated with Human Neutrophil Elastase (HNE)-DNA complexes and SOFA score at 1dpa, and were associated with mortality. Patient´s neutrophils had spontaneous NETosis and were unresponsive to stimulation. Although platelet P-selectin and TNF-α were increased in both groups, higher platelet TLR4 expression, VWF levels and IL-6 were found in septics at 1dpa. Neither platelet activation markers nor cytokines correlated with nucleosomes or HNE-DNA. Nucleosomes could be indicators of organ damage and predictors of mortality in septic but not in burn patients. Platelet activation, VWF and cytokines do not appear to be key mediators of NETosis in these patient groups.
Fil: Kaufman, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Magosevich, Débora. Clínica Sagrado Corazón; Argentina
Fil: Moreno, María Carolina. Clínica Bazterrica; Argentina
Fil: Guzman, María Alejandra. Gobiernos de la Ciudad de Buenos Aires. Hospital de Quemados Dr. Arturo Umberto Illia; Argentina
Fil: D'Atri, Lina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Carestia, Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Fandiño, María Eugenia. Clínica Sagrado Corazón; Argentina
Fil: Fondevila, Carlos. Clínica Bazterrica; Argentina
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
description NETosis is a host defense mechanism associated with inflammation and tissue damage. Experimental models show that platelets and von Willebrand factor (VWF) are key elements for intravascular NETosis. We determined NETosis in septic and burn patients at 1 and 4days post-admission (dpa). Nucleosomes were elevated in patients. In septics, they correlated with Human Neutrophil Elastase (HNE)-DNA complexes and SOFA score at 1dpa, and were associated with mortality. Patient´s neutrophils had spontaneous NETosis and were unresponsive to stimulation. Although platelet P-selectin and TNF-α were increased in both groups, higher platelet TLR4 expression, VWF levels and IL-6 were found in septics at 1dpa. Neither platelet activation markers nor cytokines correlated with nucleosomes or HNE-DNA. Nucleosomes could be indicators of organ damage and predictors of mortality in septic but not in burn patients. Platelet activation, VWF and cytokines do not appear to be key mediators of NETosis in these patient groups.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41343
Kaufman, Tomás; Magosevich, Débora; Moreno, María Carolina; Guzman, María Alejandra; D'Atri, Lina Paola; et al.; Nucleosomes and neutrophil extracellular traps in septic and burn patients; Academic Press Inc Elsevier Science; Clinical Immunology; 183; 10-2017; 254-262
1521-6616
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41343
identifier_str_mv Kaufman, Tomás; Magosevich, Débora; Moreno, María Carolina; Guzman, María Alejandra; D'Atri, Lina Paola; et al.; Nucleosomes and neutrophil extracellular traps in septic and burn patients; Academic Press Inc Elsevier Science; Clinical Immunology; 183; 10-2017; 254-262
1521-6616
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S152166161730339X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.clim.2017.08.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268652348375040
score 13.13397

Publicaciones similares