Determination of cytokeratins 1, 13 and 14 in oral lichen planus

Autores
Rivarola de Gutierrez, Emilce; Innocenti, Alicia Carolina; Cippitelli, Marìa José; Salomon, Susana; Vargas Roig, Laura Maria
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cytokeratins (CK) are molecules of the cytoskeleton that contribute to the cellular differenciation. We studied the expression of CK1, CK13 and CK14 in thirty-three patients with OLP. The biopsied lesions were located in the dorsal surface of the tongue, the palatal keratinized mucosa and the nonkeratinized buccal mucosa. Objectives: This study aimed to determine the expression of CK1, CK13 and CK14 in oral lichen planus (OLP) and its relations with: clinical patterns, prognosis, drugs and tobacco intake and histopathological features. Study Design: Immunohistochemical analysis, retrospective, descriptive, observational and no randomized study. Results: No significant difference was observed in the expression of CK1 in patients with or without drug treatment. No association was found with the amount of drugs intake or smoking nor with the histopathological features examined. Samples immunostained with CK13 were all positive in the suprabasal layers, and 13 of them in the basal layer. In these last ones, statistical analysis showed significance in the grade of vacuolization of the basal layer (p=0.023) and in the degree of exocytosis (p=0.0025), this, making the degree of affection higher for both parameters. Thirty-two tissue sections were immunostained with CK14. CK14 was expressed in the basal layer in 97% of samples and in the suprabasal layer in 94% of samples. Conclusions: The three CK were altered in OLP. CK1 does not have a direct connection with the presence of orthokeratosis. The finding of the CK13 in the basal layer is related to the agression of the lymphocytic infiltration in the epithelium, due to the basal stratum vacuolization and the increase in lymphocytic exocitosis. The presence of CK14 in the suprabasal stratums is not a parameter to predict malignancy. The CK in OLP do not follow the normal pattern of keratinized or non-keratinized mucosa.
Fil: Rivarola de Gutierrez, Emilce. Universidad Nacional de Cuyo. Facultad de Odontologia; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Innocenti, Alicia Carolina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Cippitelli, Marìa José. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Salomon, Susana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Universidad Nacional de Cuyo. Facultad de Odontologia; Argentina
Fil: Vargas Roig, Laura Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo; Argentina
Materia
CYTOKERATIN
ORAL LICHEN PLANUS
BASAL CELL VACUOLIZATION
LYMPHOCYTIC EXOCYTOSIS
CK1
CK13
CK14
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10413
Acceder
id CONICETDig_d9702820bc3d191307abcb9e3aeec029
oai_identifier_str oai:ri.conicet.gov.ar:11336/10413
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Determination of cytokeratins 1, 13 and 14 in oral lichen planusRivarola de Gutierrez, EmilceInnocenti, Alicia CarolinaCippitelli, Marìa JoséSalomon, SusanaVargas Roig, Laura MariaCYTOKERATINORAL LICHEN PLANUSBASAL CELL VACUOLIZATIONLYMPHOCYTIC EXOCYTOSISCK1CK13CK14https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Cytokeratins (CK) are molecules of the cytoskeleton that contribute to the cellular differenciation. We studied the expression of CK1, CK13 and CK14 in thirty-three patients with OLP. The biopsied lesions were located in the dorsal surface of the tongue, the palatal keratinized mucosa and the nonkeratinized buccal mucosa. Objectives: This study aimed to determine the expression of CK1, CK13 and CK14 in oral lichen planus (OLP) and its relations with: clinical patterns, prognosis, drugs and tobacco intake and histopathological features. Study Design: Immunohistochemical analysis, retrospective, descriptive, observational and no randomized study. Results: No significant difference was observed in the expression of CK1 in patients with or without drug treatment. No association was found with the amount of drugs intake or smoking nor with the histopathological features examined. Samples immunostained with CK13 were all positive in the suprabasal layers, and 13 of them in the basal layer. In these last ones, statistical analysis showed significance in the grade of vacuolization of the basal layer (p=0.023) and in the degree of exocytosis (p=0.0025), this, making the degree of affection higher for both parameters. Thirty-two tissue sections were immunostained with CK14. CK14 was expressed in the basal layer in 97% of samples and in the suprabasal layer in 94% of samples. Conclusions: The three CK were altered in OLP. CK1 does not have a direct connection with the presence of orthokeratosis. The finding of the CK13 in the basal layer is related to the agression of the lymphocytic infiltration in the epithelium, due to the basal stratum vacuolization and the increase in lymphocytic exocitosis. The presence of CK14 in the suprabasal stratums is not a parameter to predict malignancy. The CK in OLP do not follow the normal pattern of keratinized or non-keratinized mucosa.Fil: Rivarola de Gutierrez, Emilce. Universidad Nacional de Cuyo. Facultad de Odontologia; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Innocenti, Alicia Carolina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Cippitelli, Marìa José. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Salomon, Susana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Universidad Nacional de Cuyo. Facultad de Odontologia; ArgentinaFil: Vargas Roig, Laura Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo; ArgentinaMedicina Oral S L2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10413Rivarola de Gutierrez, Emilce; Innocenti, Alicia Carolina; Cippitelli, Marìa José; Salomon, Susana; Vargas Roig, Laura Maria; Determination of cytokeratins 1, 13 and 14 in oral lichen planus; Medicina Oral S L; Medicina Oral Patologia Oral y Cirugia Bucal; 19; 4; 7-2014; e359-e3651698-44471698-6946enginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4119311/info:eu-repo/semantics/altIdentifier/url/http://www.medicinaoral.com/pubmed/medoralv19_i4_p359.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:58Zoai:ri.conicet.gov.ar:11336/10413instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:58.719CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determination of cytokeratins 1, 13 and 14 in oral lichen planus
title Determination of cytokeratins 1, 13 and 14 in oral lichen planus
spellingShingle Determination of cytokeratins 1, 13 and 14 in oral lichen planus
Rivarola de Gutierrez, Emilce
CYTOKERATIN
ORAL LICHEN PLANUS
BASAL CELL VACUOLIZATION
LYMPHOCYTIC EXOCYTOSIS
CK1
CK13
CK14
title_short Determination of cytokeratins 1, 13 and 14 in oral lichen planus
title_full Determination of cytokeratins 1, 13 and 14 in oral lichen planus
title_fullStr Determination of cytokeratins 1, 13 and 14 in oral lichen planus
title_full_unstemmed Determination of cytokeratins 1, 13 and 14 in oral lichen planus
title_sort Determination of cytokeratins 1, 13 and 14 in oral lichen planus
dc.creator.none.fl_str_mv Rivarola de Gutierrez, Emilce
Innocenti, Alicia Carolina
Cippitelli, Marìa José
Salomon, Susana
Vargas Roig, Laura Maria
author Rivarola de Gutierrez, Emilce
author_facet Rivarola de Gutierrez, Emilce
Innocenti, Alicia Carolina
Cippitelli, Marìa José
Salomon, Susana
Vargas Roig, Laura Maria
author_role author
author2 Innocenti, Alicia Carolina
Cippitelli, Marìa José
Salomon, Susana
Vargas Roig, Laura Maria
author2_role author
author
author
author
dc.subject.none.fl_str_mv CYTOKERATIN
ORAL LICHEN PLANUS
BASAL CELL VACUOLIZATION
LYMPHOCYTIC EXOCYTOSIS
CK1
CK13
CK14
topic CYTOKERATIN
ORAL LICHEN PLANUS
BASAL CELL VACUOLIZATION
LYMPHOCYTIC EXOCYTOSIS
CK1
CK13
CK14
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Cytokeratins (CK) are molecules of the cytoskeleton that contribute to the cellular differenciation. We studied the expression of CK1, CK13 and CK14 in thirty-three patients with OLP. The biopsied lesions were located in the dorsal surface of the tongue, the palatal keratinized mucosa and the nonkeratinized buccal mucosa. Objectives: This study aimed to determine the expression of CK1, CK13 and CK14 in oral lichen planus (OLP) and its relations with: clinical patterns, prognosis, drugs and tobacco intake and histopathological features. Study Design: Immunohistochemical analysis, retrospective, descriptive, observational and no randomized study. Results: No significant difference was observed in the expression of CK1 in patients with or without drug treatment. No association was found with the amount of drugs intake or smoking nor with the histopathological features examined. Samples immunostained with CK13 were all positive in the suprabasal layers, and 13 of them in the basal layer. In these last ones, statistical analysis showed significance in the grade of vacuolization of the basal layer (p=0.023) and in the degree of exocytosis (p=0.0025), this, making the degree of affection higher for both parameters. Thirty-two tissue sections were immunostained with CK14. CK14 was expressed in the basal layer in 97% of samples and in the suprabasal layer in 94% of samples. Conclusions: The three CK were altered in OLP. CK1 does not have a direct connection with the presence of orthokeratosis. The finding of the CK13 in the basal layer is related to the agression of the lymphocytic infiltration in the epithelium, due to the basal stratum vacuolization and the increase in lymphocytic exocitosis. The presence of CK14 in the suprabasal stratums is not a parameter to predict malignancy. The CK in OLP do not follow the normal pattern of keratinized or non-keratinized mucosa.
Fil: Rivarola de Gutierrez, Emilce. Universidad Nacional de Cuyo. Facultad de Odontologia; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Innocenti, Alicia Carolina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Cippitelli, Marìa José. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Salomon, Susana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Universidad Nacional de Cuyo. Facultad de Odontologia; Argentina
Fil: Vargas Roig, Laura Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo; Argentina
description Cytokeratins (CK) are molecules of the cytoskeleton that contribute to the cellular differenciation. We studied the expression of CK1, CK13 and CK14 in thirty-three patients with OLP. The biopsied lesions were located in the dorsal surface of the tongue, the palatal keratinized mucosa and the nonkeratinized buccal mucosa. Objectives: This study aimed to determine the expression of CK1, CK13 and CK14 in oral lichen planus (OLP) and its relations with: clinical patterns, prognosis, drugs and tobacco intake and histopathological features. Study Design: Immunohistochemical analysis, retrospective, descriptive, observational and no randomized study. Results: No significant difference was observed in the expression of CK1 in patients with or without drug treatment. No association was found with the amount of drugs intake or smoking nor with the histopathological features examined. Samples immunostained with CK13 were all positive in the suprabasal layers, and 13 of them in the basal layer. In these last ones, statistical analysis showed significance in the grade of vacuolization of the basal layer (p=0.023) and in the degree of exocytosis (p=0.0025), this, making the degree of affection higher for both parameters. Thirty-two tissue sections were immunostained with CK14. CK14 was expressed in the basal layer in 97% of samples and in the suprabasal layer in 94% of samples. Conclusions: The three CK were altered in OLP. CK1 does not have a direct connection with the presence of orthokeratosis. The finding of the CK13 in the basal layer is related to the agression of the lymphocytic infiltration in the epithelium, due to the basal stratum vacuolization and the increase in lymphocytic exocitosis. The presence of CK14 in the suprabasal stratums is not a parameter to predict malignancy. The CK in OLP do not follow the normal pattern of keratinized or non-keratinized mucosa.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10413
Rivarola de Gutierrez, Emilce; Innocenti, Alicia Carolina; Cippitelli, Marìa José; Salomon, Susana; Vargas Roig, Laura Maria; Determination of cytokeratins 1, 13 and 14 in oral lichen planus; Medicina Oral S L; Medicina Oral Patologia Oral y Cirugia Bucal; 19; 4; 7-2014; e359-e365
1698-4447
1698-6946
url http://hdl.handle.net/11336/10413
identifier_str_mv Rivarola de Gutierrez, Emilce; Innocenti, Alicia Carolina; Cippitelli, Marìa José; Salomon, Susana; Vargas Roig, Laura Maria; Determination of cytokeratins 1, 13 and 14 in oral lichen planus; Medicina Oral S L; Medicina Oral Patologia Oral y Cirugia Bucal; 19; 4; 7-2014; e359-e365
1698-4447
1698-6946
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4119311/
info:eu-repo/semantics/altIdentifier/url/http://www.medicinaoral.com/pubmed/medoralv19_i4_p359.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Medicina Oral S L
publisher.none.fl_str_mv Medicina Oral S L
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614379426807808
score 13.070432

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