Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cells

Autores
Rossi, Soledad Paola; Walenta, Lena; Rey Ares, Veronica; Köhn, Frank-Michael; Ulrich Schwarzer, J.; Welter, Harald; Calandra, Ricardo Saul; Frungieri, Monica Beatriz; Mayerhofer, Artur
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Stress activates the sympathetic nervous system and is linked to impaired fertility in man. We hypothesized that catecholamines by acting on testicular cells have a role in these events, possibly by fostering an inflammatory environment. The cells of the wall of seminiferous tubules, human testicular peritubular cells (HTPCs), express adrenergic receptors (ADRs) α1B, α1D, β1 and β2. A selective α1-ADR agonist, phenylephrine, increased intracellular Ca 2+ -levels in cultured HTPCs and induced COX-2, IL-6 and MCP-1 mRNA expression without affecting IL-1β mRNA. These changes were paralleled by a significant increase in the secretion of IL-6 and MCP-1. Epinephrine was also effective, but salbutamol, a selective β2-ADR agonist was not. Our results suggest that stress-associated elevation of catecholamines may be able to promote inflammatory events by targeting peritubular cells in the human testis. Blockage of α1-ADRs may therefore be a novel way to interfere with stress-related impairment of male reproductive functions.
Fil: Rossi, Soledad Paola. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Walenta, Lena. Ludwig Maximilians Universitat; Alemania
Fil: Rey Ares, Veronica. Ludwig Maximilians Universitat; Alemania
Fil: Köhn, Frank-Michael. Andrologicum; Alemania
Fil: Ulrich Schwarzer, J.. Andrology Center Munich; Alemania
Fil: Welter, Harald. Ludwig Maximilians Universitat; Alemania
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Frungieri, Monica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Mayerhofer, Artur. Ludwig Maximilians Universitat; Alemania
Materia
ADRENERGIC RECEPTORS
EPINEPHRINE
INFERTILITY
INFLAMMATION
MALE FERTILITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/91072
Acceder
id CONICETDig_d8cde83942002ee7be83b13553be3925
oai_identifier_str oai:ri.conicet.gov.ar:11336/91072
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cellsRossi, Soledad PaolaWalenta, LenaRey Ares, VeronicaKöhn, Frank-MichaelUlrich Schwarzer, J.Welter, HaraldCalandra, Ricardo SaulFrungieri, Monica BeatrizMayerhofer, ArturADRENERGIC RECEPTORSEPINEPHRINEINFERTILITYINFLAMMATIONMALE FERTILITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Stress activates the sympathetic nervous system and is linked to impaired fertility in man. We hypothesized that catecholamines by acting on testicular cells have a role in these events, possibly by fostering an inflammatory environment. The cells of the wall of seminiferous tubules, human testicular peritubular cells (HTPCs), express adrenergic receptors (ADRs) α1B, α1D, β1 and β2. A selective α1-ADR agonist, phenylephrine, increased intracellular Ca 2+ -levels in cultured HTPCs and induced COX-2, IL-6 and MCP-1 mRNA expression without affecting IL-1β mRNA. These changes were paralleled by a significant increase in the secretion of IL-6 and MCP-1. Epinephrine was also effective, but salbutamol, a selective β2-ADR agonist was not. Our results suggest that stress-associated elevation of catecholamines may be able to promote inflammatory events by targeting peritubular cells in the human testis. Blockage of α1-ADRs may therefore be a novel way to interfere with stress-related impairment of male reproductive functions.Fil: Rossi, Soledad Paola. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Walenta, Lena. Ludwig Maximilians Universitat; AlemaniaFil: Rey Ares, Veronica. Ludwig Maximilians Universitat; AlemaniaFil: Köhn, Frank-Michael. Andrologicum; AlemaniaFil: Ulrich Schwarzer, J.. Andrology Center Munich; AlemaniaFil: Welter, Harald. Ludwig Maximilians Universitat; AlemaniaFil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Frungieri, Monica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Mayerhofer, Artur. Ludwig Maximilians Universitat; AlemaniaElsevier Ireland2018-10-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91072Rossi, Soledad Paola; Walenta, Lena; Rey Ares, Veronica; Köhn, Frank-Michael; Ulrich Schwarzer, J.; et al.; Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cells; Elsevier Ireland; Molecular and Cellular Endocrinology; 474; 15-10-2018; 1-90303-7207CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0303720718300509?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2018.01.027info:eu-repo/semantics/altIdentifier/pmid/https://www.ncbi.nlm.nih.gov/pubmed/29407194info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:50:45Zoai:ri.conicet.gov.ar:11336/91072instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:50:45.704CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cells
title Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cells
spellingShingle Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cells
Rossi, Soledad Paola
ADRENERGIC RECEPTORS
EPINEPHRINE
INFERTILITY
INFLAMMATION
MALE FERTILITY
title_short Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cells
title_full Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cells
title_fullStr Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cells
title_full_unstemmed Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cells
title_sort Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cells
dc.creator.none.fl_str_mv Rossi, Soledad Paola
Walenta, Lena
Rey Ares, Veronica
Köhn, Frank-Michael
Ulrich Schwarzer, J.
Welter, Harald
Calandra, Ricardo Saul
Frungieri, Monica Beatriz
Mayerhofer, Artur
author Rossi, Soledad Paola
author_facet Rossi, Soledad Paola
Walenta, Lena
Rey Ares, Veronica
Köhn, Frank-Michael
Ulrich Schwarzer, J.
Welter, Harald
Calandra, Ricardo Saul
Frungieri, Monica Beatriz
Mayerhofer, Artur
author_role author
author2 Walenta, Lena
Rey Ares, Veronica
Köhn, Frank-Michael
Ulrich Schwarzer, J.
Welter, Harald
Calandra, Ricardo Saul
Frungieri, Monica Beatriz
Mayerhofer, Artur
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ADRENERGIC RECEPTORS
EPINEPHRINE
INFERTILITY
INFLAMMATION
MALE FERTILITY
topic ADRENERGIC RECEPTORS
EPINEPHRINE
INFERTILITY
INFLAMMATION
MALE FERTILITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Stress activates the sympathetic nervous system and is linked to impaired fertility in man. We hypothesized that catecholamines by acting on testicular cells have a role in these events, possibly by fostering an inflammatory environment. The cells of the wall of seminiferous tubules, human testicular peritubular cells (HTPCs), express adrenergic receptors (ADRs) α1B, α1D, β1 and β2. A selective α1-ADR agonist, phenylephrine, increased intracellular Ca 2+ -levels in cultured HTPCs and induced COX-2, IL-6 and MCP-1 mRNA expression without affecting IL-1β mRNA. These changes were paralleled by a significant increase in the secretion of IL-6 and MCP-1. Epinephrine was also effective, but salbutamol, a selective β2-ADR agonist was not. Our results suggest that stress-associated elevation of catecholamines may be able to promote inflammatory events by targeting peritubular cells in the human testis. Blockage of α1-ADRs may therefore be a novel way to interfere with stress-related impairment of male reproductive functions.
Fil: Rossi, Soledad Paola. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Walenta, Lena. Ludwig Maximilians Universitat; Alemania
Fil: Rey Ares, Veronica. Ludwig Maximilians Universitat; Alemania
Fil: Köhn, Frank-Michael. Andrologicum; Alemania
Fil: Ulrich Schwarzer, J.. Andrology Center Munich; Alemania
Fil: Welter, Harald. Ludwig Maximilians Universitat; Alemania
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Frungieri, Monica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Mayerhofer, Artur. Ludwig Maximilians Universitat; Alemania
description Stress activates the sympathetic nervous system and is linked to impaired fertility in man. We hypothesized that catecholamines by acting on testicular cells have a role in these events, possibly by fostering an inflammatory environment. The cells of the wall of seminiferous tubules, human testicular peritubular cells (HTPCs), express adrenergic receptors (ADRs) α1B, α1D, β1 and β2. A selective α1-ADR agonist, phenylephrine, increased intracellular Ca 2+ -levels in cultured HTPCs and induced COX-2, IL-6 and MCP-1 mRNA expression without affecting IL-1β mRNA. These changes were paralleled by a significant increase in the secretion of IL-6 and MCP-1. Epinephrine was also effective, but salbutamol, a selective β2-ADR agonist was not. Our results suggest that stress-associated elevation of catecholamines may be able to promote inflammatory events by targeting peritubular cells in the human testis. Blockage of α1-ADRs may therefore be a novel way to interfere with stress-related impairment of male reproductive functions.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/91072
Rossi, Soledad Paola; Walenta, Lena; Rey Ares, Veronica; Köhn, Frank-Michael; Ulrich Schwarzer, J.; et al.; Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cells; Elsevier Ireland; Molecular and Cellular Endocrinology; 474; 15-10-2018; 1-9
0303-7207
CONICET Digital
CONICET
url http://hdl.handle.net/11336/91072
identifier_str_mv Rossi, Soledad Paola; Walenta, Lena; Rey Ares, Veronica; Köhn, Frank-Michael; Ulrich Schwarzer, J.; et al.; Alpha 1 adrenergic receptor-mediated inflammatory responses in human testicular peritubular cells; Elsevier Ireland; Molecular and Cellular Endocrinology; 474; 15-10-2018; 1-9
0303-7207
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0303720718300509?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2018.01.027
info:eu-repo/semantics/altIdentifier/pmid/https://www.ncbi.nlm.nih.gov/pubmed/29407194
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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