Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines

Autores
Gargiulo, Lucía; Copsel, Sabrina Natalia; Rivero, Ezequiel Mariano; Galés, Céline; Sénard, Jean Michel; Luthy, Isabel Alicia; Davio, Carlos Alberto; Bruzzone, Ariana
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Breast cancer is the most frequent malignancy in women. Several reports demonstrated that adrenergic receptors (ARs) are involved in breast cancer. Here we observed that epinephrine (Epi), an endogenous AR agonist, caused opposite effects in non-tumorigenic (MCF-10A and HBL-100) and tumor cells (MCF-7 and MDA-MB-231). Thus, Epi, in non-tumor breast cells, as well as isoproterenol (β-agonist), in all cell lines, maintained a benign phenotype, decreasing cell proliferation and migration, and stimulating cell adhesion. β-AR expression and cAMP levels were higher in MCF-10A than in MCF-7 cells. β₂-AR knock-down caused a significant increase of cell proliferation and migration, and a decrease of cell adhesion both in basal and in Iso-stimulated conditions. Coincidently, β₂-AR over-expression induced a significant decrease of cell proliferation and migration, and an increase of cell adhesion. Therefore, β₂-AR is implied in cell phenotype and its agonists or antagonists could eventually complement cancer therapy.
Fil: Gargiulo, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Copsel, Sabrina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
Fil: Rivero, Ezequiel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Galés, Céline. Inserm; Francia
Fil: Sénard, Jean Michel. Inserm; Francia
Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Davio, Carlos Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
Fil: Bruzzone, Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
HUMAN BREAST CANCER CELLS
NON-TUMORIGENIC BREAST CELLS
EPINEPHRINE
ADRENERGIC RECEPTORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22922
Acceder
id CONICETDig_6688e4550ac562341565b88957198e00
oai_identifier_str oai:ri.conicet.gov.ar:11336/22922
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell linesGargiulo, LucíaCopsel, Sabrina NataliaRivero, Ezequiel MarianoGalés, CélineSénard, Jean MichelLuthy, Isabel AliciaDavio, Carlos AlbertoBruzzone, ArianaHUMAN BREAST CANCER CELLSNON-TUMORIGENIC BREAST CELLSEPINEPHRINEADRENERGIC RECEPTORShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Breast cancer is the most frequent malignancy in women. Several reports demonstrated that adrenergic receptors (ARs) are involved in breast cancer. Here we observed that epinephrine (Epi), an endogenous AR agonist, caused opposite effects in non-tumorigenic (MCF-10A and HBL-100) and tumor cells (MCF-7 and MDA-MB-231). Thus, Epi, in non-tumor breast cells, as well as isoproterenol (β-agonist), in all cell lines, maintained a benign phenotype, decreasing cell proliferation and migration, and stimulating cell adhesion. β-AR expression and cAMP levels were higher in MCF-10A than in MCF-7 cells. β₂-AR knock-down caused a significant increase of cell proliferation and migration, and a decrease of cell adhesion both in basal and in Iso-stimulated conditions. Coincidently, β₂-AR over-expression induced a significant decrease of cell proliferation and migration, and an increase of cell adhesion. Therefore, β₂-AR is implied in cell phenotype and its agonists or antagonists could eventually complement cancer therapy.Fil: Gargiulo, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Copsel, Sabrina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; ArgentinaFil: Rivero, Ezequiel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Galés, Céline. Inserm; FranciaFil: Sénard, Jean Michel. Inserm; FranciaFil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Davio, Carlos Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; ArgentinaFil: Bruzzone, Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaImpact Journals2014-11-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22922Gargiulo, Lucía; Copsel, Sabrina Natalia; Rivero, Ezequiel Mariano; Galés, Céline; Sénard, Jean Michel; et al.; Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines; Impact Journals; Oncotarget; 5; 20; 7-11-2014; 10058-100691949-25531949-2553CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259405/info:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.2460info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:48Zoai:ri.conicet.gov.ar:11336/22922instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:48.473CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines
title Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines
spellingShingle Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines
Gargiulo, Lucía
HUMAN BREAST CANCER CELLS
NON-TUMORIGENIC BREAST CELLS
EPINEPHRINE
ADRENERGIC RECEPTORS
title_short Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines
title_full Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines
title_fullStr Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines
title_full_unstemmed Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines
title_sort Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines
dc.creator.none.fl_str_mv Gargiulo, Lucía
Copsel, Sabrina Natalia
Rivero, Ezequiel Mariano
Galés, Céline
Sénard, Jean Michel
Luthy, Isabel Alicia
Davio, Carlos Alberto
Bruzzone, Ariana
author Gargiulo, Lucía
author_facet Gargiulo, Lucía
Copsel, Sabrina Natalia
Rivero, Ezequiel Mariano
Galés, Céline
Sénard, Jean Michel
Luthy, Isabel Alicia
Davio, Carlos Alberto
Bruzzone, Ariana
author_role author
author2 Copsel, Sabrina Natalia
Rivero, Ezequiel Mariano
Galés, Céline
Sénard, Jean Michel
Luthy, Isabel Alicia
Davio, Carlos Alberto
Bruzzone, Ariana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HUMAN BREAST CANCER CELLS
NON-TUMORIGENIC BREAST CELLS
EPINEPHRINE
ADRENERGIC RECEPTORS
topic HUMAN BREAST CANCER CELLS
NON-TUMORIGENIC BREAST CELLS
EPINEPHRINE
ADRENERGIC RECEPTORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Breast cancer is the most frequent malignancy in women. Several reports demonstrated that adrenergic receptors (ARs) are involved in breast cancer. Here we observed that epinephrine (Epi), an endogenous AR agonist, caused opposite effects in non-tumorigenic (MCF-10A and HBL-100) and tumor cells (MCF-7 and MDA-MB-231). Thus, Epi, in non-tumor breast cells, as well as isoproterenol (β-agonist), in all cell lines, maintained a benign phenotype, decreasing cell proliferation and migration, and stimulating cell adhesion. β-AR expression and cAMP levels were higher in MCF-10A than in MCF-7 cells. β₂-AR knock-down caused a significant increase of cell proliferation and migration, and a decrease of cell adhesion both in basal and in Iso-stimulated conditions. Coincidently, β₂-AR over-expression induced a significant decrease of cell proliferation and migration, and an increase of cell adhesion. Therefore, β₂-AR is implied in cell phenotype and its agonists or antagonists could eventually complement cancer therapy.
Fil: Gargiulo, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Copsel, Sabrina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
Fil: Rivero, Ezequiel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Galés, Céline. Inserm; Francia
Fil: Sénard, Jean Michel. Inserm; Francia
Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Davio, Carlos Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
Fil: Bruzzone, Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Breast cancer is the most frequent malignancy in women. Several reports demonstrated that adrenergic receptors (ARs) are involved in breast cancer. Here we observed that epinephrine (Epi), an endogenous AR agonist, caused opposite effects in non-tumorigenic (MCF-10A and HBL-100) and tumor cells (MCF-7 and MDA-MB-231). Thus, Epi, in non-tumor breast cells, as well as isoproterenol (β-agonist), in all cell lines, maintained a benign phenotype, decreasing cell proliferation and migration, and stimulating cell adhesion. β-AR expression and cAMP levels were higher in MCF-10A than in MCF-7 cells. β₂-AR knock-down caused a significant increase of cell proliferation and migration, and a decrease of cell adhesion both in basal and in Iso-stimulated conditions. Coincidently, β₂-AR over-expression induced a significant decrease of cell proliferation and migration, and an increase of cell adhesion. Therefore, β₂-AR is implied in cell phenotype and its agonists or antagonists could eventually complement cancer therapy.
publishDate 2014
dc.date.none.fl_str_mv 2014-11-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22922
Gargiulo, Lucía; Copsel, Sabrina Natalia; Rivero, Ezequiel Mariano; Galés, Céline; Sénard, Jean Michel; et al.; Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines; Impact Journals; Oncotarget; 5; 20; 7-11-2014; 10058-10069
1949-2553
1949-2553
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22922
identifier_str_mv Gargiulo, Lucía; Copsel, Sabrina Natalia; Rivero, Ezequiel Mariano; Galés, Céline; Sénard, Jean Michel; et al.; Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines; Impact Journals; Oncotarget; 5; 20; 7-11-2014; 10058-10069
1949-2553
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259405/
info:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.2460
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Impact Journals
publisher.none.fl_str_mv Impact Journals
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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