Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patients

Autores
Kampfer, C.; Spillner, S.; Spinnler, K.; Schwarzer, J. U.; Terradas, C.; Ponzio, R.; Puigdomenech, E.; Levalle, O.; Köhn, F. M.; Matzkin, Maria Eugenia; Calandra, Ricardo Saul; Frungieri, Monica Beatriz; Mayerhofer, Artur
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fibrosis, increased amounts of immune cells and expression of COX-2 in the testes of infertility patients provide circumstantial evidence for a specific testicular milieu, in which reactive oxygen species (ROS) could be increased. If ROS level increase and/or ROS scavengers decrease, the resulting testicular oxidative stress may contribute to human male infertility. Primary peritubular cells of the human testis, from men with normal spermatogenesis (HTPCs) and infertile patients (HTPC-Fs), previously allowed us to identify an end product of COX-2 action, a prostaglandin derivative (15dPGJ2), which acts via ROS to alter the phenotype of peritubular cells, at least in vitro. Using testicular biopsies we now found 15dPGJ2 in patients and hence we started exploring the ROS scavenger systems of the human testis. This system includes catalase, DJ-1, peroxiredoxin 1, SOD 1 and 2, glutathione-S-transferase and HMOX-1, which were identified by RT-PCR/sequencing in HTPCs and HTPC-Fs and whole testes. Catalase, DJ-1, peroxiredoxin 1 and SOD 2 were also detected by Western blots and in part by immunohistochemistry in testicular samples. Western blots of cultured cells further revealed that catalase levels, but not peroxiredoxin 1, SOD 2 or DJ-1 levels, are significantly higher in HTPC-Fs than in HTPCs. This particular difference is correlated with the improved ability of HTPC-Fs to handle ROS, which became evident when cells were exposed to 100 μm H2O2. H2O2 induced stronger responses in HTPCs than in HTPC-Fs, which correlates with the lower level of the H2O2-degrading defence enzyme catalase in HTPCs. The results provide evidence for an adaptation to elevated ROS levels, which must have occurred in vivo and which persist in vitro in HTPC-Fs. Thus, in infertile men with impaired spermatogenesis elevated ROS levels likely exist, at least in the tubular wall.
Fil: Kampfer, C.. Ludwig Maximilians Universitat; Alemania
Fil: Spillner, S.. Ludwig Maximilians Universitat; Alemania
Fil: Spinnler, K.. Ludwig Maximilians Universitat; Alemania
Fil: Schwarzer, J. U.. Praxis for Urology and Andrology; Alemania
Fil: Terradas, C.. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Carlos Durand; Argentina
Fil: Ponzio, R.. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Puigdomenech, E.. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Levalle, O.. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Carlos Durand; Argentina
Fil: Köhn, F. M.. Andrologicum Müenchen; Alemania
Fil: Matzkin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Frungieri, Monica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Mayerhofer, Artur. Ludwig Maximilians Universitat; Alemania
Materia
INFERTILITY
TESTICULAR BIOPSY
OXIDATIVE STRESS
PRIMATES
ANIMAL MODELS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/270182
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/270182
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patientsKampfer, C.Spillner, S.Spinnler, K.Schwarzer, J. U.Terradas, C.Ponzio, R.Puigdomenech, E.Levalle, O.Köhn, F. M.Matzkin, Maria EugeniaCalandra, Ricardo SaulFrungieri, Monica BeatrizMayerhofer, ArturINFERTILITYTESTICULAR BIOPSYOXIDATIVE STRESSPRIMATESANIMAL MODELShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fibrosis, increased amounts of immune cells and expression of COX-2 in the testes of infertility patients provide circumstantial evidence for a specific testicular milieu, in which reactive oxygen species (ROS) could be increased. If ROS level increase and/or ROS scavengers decrease, the resulting testicular oxidative stress may contribute to human male infertility. Primary peritubular cells of the human testis, from men with normal spermatogenesis (HTPCs) and infertile patients (HTPC-Fs), previously allowed us to identify an end product of COX-2 action, a prostaglandin derivative (15dPGJ2), which acts via ROS to alter the phenotype of peritubular cells, at least in vitro. Using testicular biopsies we now found 15dPGJ2 in patients and hence we started exploring the ROS scavenger systems of the human testis. This system includes catalase, DJ-1, peroxiredoxin 1, SOD 1 and 2, glutathione-S-transferase and HMOX-1, which were identified by RT-PCR/sequencing in HTPCs and HTPC-Fs and whole testes. Catalase, DJ-1, peroxiredoxin 1 and SOD 2 were also detected by Western blots and in part by immunohistochemistry in testicular samples. Western blots of cultured cells further revealed that catalase levels, but not peroxiredoxin 1, SOD 2 or DJ-1 levels, are significantly higher in HTPC-Fs than in HTPCs. This particular difference is correlated with the improved ability of HTPC-Fs to handle ROS, which became evident when cells were exposed to 100 μm H2O2. H2O2 induced stronger responses in HTPCs than in HTPC-Fs, which correlates with the lower level of the H2O2-degrading defence enzyme catalase in HTPCs. The results provide evidence for an adaptation to elevated ROS levels, which must have occurred in vivo and which persist in vitro in HTPC-Fs. Thus, in infertile men with impaired spermatogenesis elevated ROS levels likely exist, at least in the tubular wall.Fil: Kampfer, C.. Ludwig Maximilians Universitat; AlemaniaFil: Spillner, S.. Ludwig Maximilians Universitat; AlemaniaFil: Spinnler, K.. Ludwig Maximilians Universitat; AlemaniaFil: Schwarzer, J. U.. Praxis for Urology and Andrology; AlemaniaFil: Terradas, C.. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Carlos Durand; ArgentinaFil: Ponzio, R.. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Puigdomenech, E.. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Levalle, O.. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Carlos Durand; ArgentinaFil: Köhn, F. M.. Andrologicum Müenchen; AlemaniaFil: Matzkin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Frungieri, Monica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Mayerhofer, Artur. Ludwig Maximilians Universitat; AlemaniaWiley Blackwell Publishing, Inc2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/270182Kampfer, C.; Spillner, S.; Spinnler, K.; Schwarzer, J. U.; Terradas, C.; et al.; Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patients; Wiley Blackwell Publishing, Inc; International Journal Of Andrology; 35; 6; 5-2012; 793-8010105-6263CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2605.2012.01281.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2605.2012.01281.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:36:32Zoai:ri.conicet.gov.ar:11336/270182instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:36:33.111CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patients
title Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patients
spellingShingle Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patients
Kampfer, C.
INFERTILITY
TESTICULAR BIOPSY
OXIDATIVE STRESS
PRIMATES
ANIMAL MODELS
title_short Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patients
title_full Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patients
title_fullStr Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patients
title_full_unstemmed Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patients
title_sort Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patients
dc.creator.none.fl_str_mv Kampfer, C.
Spillner, S.
Spinnler, K.
Schwarzer, J. U.
Terradas, C.
Ponzio, R.
Puigdomenech, E.
Levalle, O.
Köhn, F. M.
Matzkin, Maria Eugenia
Calandra, Ricardo Saul
Frungieri, Monica Beatriz
Mayerhofer, Artur
author Kampfer, C.
author_facet Kampfer, C.
Spillner, S.
Spinnler, K.
Schwarzer, J. U.
Terradas, C.
Ponzio, R.
Puigdomenech, E.
Levalle, O.
Köhn, F. M.
Matzkin, Maria Eugenia
Calandra, Ricardo Saul
Frungieri, Monica Beatriz
Mayerhofer, Artur
author_role author
author2 Spillner, S.
Spinnler, K.
Schwarzer, J. U.
Terradas, C.
Ponzio, R.
Puigdomenech, E.
Levalle, O.
Köhn, F. M.
Matzkin, Maria Eugenia
Calandra, Ricardo Saul
Frungieri, Monica Beatriz
Mayerhofer, Artur
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv INFERTILITY
TESTICULAR BIOPSY
OXIDATIVE STRESS
PRIMATES
ANIMAL MODELS
topic INFERTILITY
TESTICULAR BIOPSY
OXIDATIVE STRESS
PRIMATES
ANIMAL MODELS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fibrosis, increased amounts of immune cells and expression of COX-2 in the testes of infertility patients provide circumstantial evidence for a specific testicular milieu, in which reactive oxygen species (ROS) could be increased. If ROS level increase and/or ROS scavengers decrease, the resulting testicular oxidative stress may contribute to human male infertility. Primary peritubular cells of the human testis, from men with normal spermatogenesis (HTPCs) and infertile patients (HTPC-Fs), previously allowed us to identify an end product of COX-2 action, a prostaglandin derivative (15dPGJ2), which acts via ROS to alter the phenotype of peritubular cells, at least in vitro. Using testicular biopsies we now found 15dPGJ2 in patients and hence we started exploring the ROS scavenger systems of the human testis. This system includes catalase, DJ-1, peroxiredoxin 1, SOD 1 and 2, glutathione-S-transferase and HMOX-1, which were identified by RT-PCR/sequencing in HTPCs and HTPC-Fs and whole testes. Catalase, DJ-1, peroxiredoxin 1 and SOD 2 were also detected by Western blots and in part by immunohistochemistry in testicular samples. Western blots of cultured cells further revealed that catalase levels, but not peroxiredoxin 1, SOD 2 or DJ-1 levels, are significantly higher in HTPC-Fs than in HTPCs. This particular difference is correlated with the improved ability of HTPC-Fs to handle ROS, which became evident when cells were exposed to 100 μm H2O2. H2O2 induced stronger responses in HTPCs than in HTPC-Fs, which correlates with the lower level of the H2O2-degrading defence enzyme catalase in HTPCs. The results provide evidence for an adaptation to elevated ROS levels, which must have occurred in vivo and which persist in vitro in HTPC-Fs. Thus, in infertile men with impaired spermatogenesis elevated ROS levels likely exist, at least in the tubular wall.
Fil: Kampfer, C.. Ludwig Maximilians Universitat; Alemania
Fil: Spillner, S.. Ludwig Maximilians Universitat; Alemania
Fil: Spinnler, K.. Ludwig Maximilians Universitat; Alemania
Fil: Schwarzer, J. U.. Praxis for Urology and Andrology; Alemania
Fil: Terradas, C.. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Carlos Durand; Argentina
Fil: Ponzio, R.. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Puigdomenech, E.. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Levalle, O.. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Carlos Durand; Argentina
Fil: Köhn, F. M.. Andrologicum Müenchen; Alemania
Fil: Matzkin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Frungieri, Monica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina
Fil: Mayerhofer, Artur. Ludwig Maximilians Universitat; Alemania
description Fibrosis, increased amounts of immune cells and expression of COX-2 in the testes of infertility patients provide circumstantial evidence for a specific testicular milieu, in which reactive oxygen species (ROS) could be increased. If ROS level increase and/or ROS scavengers decrease, the resulting testicular oxidative stress may contribute to human male infertility. Primary peritubular cells of the human testis, from men with normal spermatogenesis (HTPCs) and infertile patients (HTPC-Fs), previously allowed us to identify an end product of COX-2 action, a prostaglandin derivative (15dPGJ2), which acts via ROS to alter the phenotype of peritubular cells, at least in vitro. Using testicular biopsies we now found 15dPGJ2 in patients and hence we started exploring the ROS scavenger systems of the human testis. This system includes catalase, DJ-1, peroxiredoxin 1, SOD 1 and 2, glutathione-S-transferase and HMOX-1, which were identified by RT-PCR/sequencing in HTPCs and HTPC-Fs and whole testes. Catalase, DJ-1, peroxiredoxin 1 and SOD 2 were also detected by Western blots and in part by immunohistochemistry in testicular samples. Western blots of cultured cells further revealed that catalase levels, but not peroxiredoxin 1, SOD 2 or DJ-1 levels, are significantly higher in HTPC-Fs than in HTPCs. This particular difference is correlated with the improved ability of HTPC-Fs to handle ROS, which became evident when cells were exposed to 100 μm H2O2. H2O2 induced stronger responses in HTPCs than in HTPC-Fs, which correlates with the lower level of the H2O2-degrading defence enzyme catalase in HTPCs. The results provide evidence for an adaptation to elevated ROS levels, which must have occurred in vivo and which persist in vitro in HTPC-Fs. Thus, in infertile men with impaired spermatogenesis elevated ROS levels likely exist, at least in the tubular wall.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/270182
Kampfer, C.; Spillner, S.; Spinnler, K.; Schwarzer, J. U.; Terradas, C.; et al.; Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patients; Wiley Blackwell Publishing, Inc; International Journal Of Andrology; 35; 6; 5-2012; 793-801
0105-6263
CONICET Digital
CONICET
url http://hdl.handle.net/11336/270182
identifier_str_mv Kampfer, C.; Spillner, S.; Spinnler, K.; Schwarzer, J. U.; Terradas, C.; et al.; Evidence for an adaptation in ROS scavenging systems in human testicular peritubular cells from infertility patients; Wiley Blackwell Publishing, Inc; International Journal Of Andrology; 35; 6; 5-2012; 793-801
0105-6263
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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