Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows

Autores
Minuti, Andrea; Zhou, Zheng; Graugnard, Daniel E; Rodriguez Zas, Sandra L.; Palladino, Rafael Alejandro; Cardoso, Felipe C.; Trevisi, Erminio; Loor, Juan J
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The study investigated the effect of an intramammary lipopolysaccharide (LPS) challenge on the bovine mammary and liver transcriptome and its consequences on metabolic biomarkers and liver tissue composition. At 7 days of lactation, 7 cows served as controls (CTR) and 7 cows (LPS) received an intramammary Escherichia coli LPS challenge. The mammary and liver tissues for transcriptomic profiling were biopsied at 2.5 h from challenge. Liver composition was evaluated at 2.5 h and 7 days after challenge, and blood biomarkers were analyzed at 2, 3, 7 and 14 days from challenge. In mammary tissue, the LPS challenge resulted in 189 differentially expressed genes (DEG), with 20 down-regulated and 169 up-regulated. In liver tissue, there were 107 DEG in LPS compared with CTR with 42 down-regulated and 65 up-regulated. In mammary, bioinformatics analysis highlighted that LPS led to activation of NOD-like receptor signaling, Toll-like receptor signaling, RIG-I-like receptor signaling and apoptosis pathways. In liver, LPS resulted in an overall inhibition of fatty acid elongation in mitochondria and activation of the p53 signaling pathway. The LPS challenge induced changes in liver lipid composition, a systemic inflammation (rise of blood ceruloplasmin and bilirubin), and an increase in body fat mobilization. The data suggest that cells within the inflamed mammary gland respond by activating mechanisms of pathogen recognition. However, in the liver the response likely depends on mediators originating from the udder that affect liver functionality and specifically fatty acid metabolism (b-oxidation, ketogenesis, and lipoprotein synthesis).
Fil: Minuti, Andrea. Universita Cattolica del Sacro Cuore; Italia
Fil: Zhou, Zheng. University Of Illinois At Urbana; Estados Unidos
Fil: Graugnard, Daniel E. University Of Illinois At Urbana; Estados Unidos
Fil: Rodriguez Zas, Sandra L.. University Of Illinois At Urbana; Estados Unidos
Fil: Palladino, Rafael Alejandro. Universidad de Buenos Aires. Facultad de Agronomia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardoso, Felipe C.. University Of Illinois At Urbana; Estados Unidos
Fil: Trevisi, Erminio. Universita Cattolica del Sacro Cuore; Italia
Fil: Loor, Juan J. University Of Illinois At Urbana; Estados Unidos
Materia
MAMMARY
LIVER
LPS
EARLY LACTATION COWS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16818
Acceder
id CONICETDig_d892f8ea5ceff3cf128c0681c406f2fd
oai_identifier_str oai:ri.conicet.gov.ar:11336/16818
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cowsMinuti, AndreaZhou, ZhengGraugnard, Daniel ERodriguez Zas, Sandra L.Palladino, Rafael AlejandroCardoso, Felipe C.Trevisi, ErminioLoor, Juan JMAMMARYLIVERLPSEARLY LACTATION COWShttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4The study investigated the effect of an intramammary lipopolysaccharide (LPS) challenge on the bovine mammary and liver transcriptome and its consequences on metabolic biomarkers and liver tissue composition. At 7 days of lactation, 7 cows served as controls (CTR) and 7 cows (LPS) received an intramammary Escherichia coli LPS challenge. The mammary and liver tissues for transcriptomic profiling were biopsied at 2.5 h from challenge. Liver composition was evaluated at 2.5 h and 7 days after challenge, and blood biomarkers were analyzed at 2, 3, 7 and 14 days from challenge. In mammary tissue, the LPS challenge resulted in 189 differentially expressed genes (DEG), with 20 down-regulated and 169 up-regulated. In liver tissue, there were 107 DEG in LPS compared with CTR with 42 down-regulated and 65 up-regulated. In mammary, bioinformatics analysis highlighted that LPS led to activation of NOD-like receptor signaling, Toll-like receptor signaling, RIG-I-like receptor signaling and apoptosis pathways. In liver, LPS resulted in an overall inhibition of fatty acid elongation in mitochondria and activation of the p53 signaling pathway. The LPS challenge induced changes in liver lipid composition, a systemic inflammation (rise of blood ceruloplasmin and bilirubin), and an increase in body fat mobilization. The data suggest that cells within the inflamed mammary gland respond by activating mechanisms of pathogen recognition. However, in the liver the response likely depends on mediators originating from the udder that affect liver functionality and specifically fatty acid metabolism (b-oxidation, ketogenesis, and lipoprotein synthesis).Fil: Minuti, Andrea. Universita Cattolica del Sacro Cuore; ItaliaFil: Zhou, Zheng. University Of Illinois At Urbana; Estados UnidosFil: Graugnard, Daniel E. University Of Illinois At Urbana; Estados UnidosFil: Rodriguez Zas, Sandra L.. University Of Illinois At Urbana; Estados UnidosFil: Palladino, Rafael Alejandro. Universidad de Buenos Aires. Facultad de Agronomia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cardoso, Felipe C.. University Of Illinois At Urbana; Estados UnidosFil: Trevisi, Erminio. Universita Cattolica del Sacro Cuore; ItaliaFil: Loor, Juan J. University Of Illinois At Urbana; Estados UnidosAmerican Physiological Society2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16818Minuti, Andrea; Zhou, Zheng; Graugnard, Daniel E; Rodriguez Zas, Sandra L.; Palladino, Rafael Alejandro; et al.; Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows; American Physiological Society; Physiological Reports; 3; 4; 4-2015; 1-12; e123882051-817Xenginfo:eu-repo/semantics/altIdentifier/doi/10.14814/phy2.12388info:eu-repo/semantics/altIdentifier/url/http://physreports.physiology.org/content/3/4/e12388info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:29Zoai:ri.conicet.gov.ar:11336/16818instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:29.471CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows
title Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows
spellingShingle Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows
Minuti, Andrea
MAMMARY
LIVER
LPS
EARLY LACTATION COWS
title_short Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows
title_full Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows
title_fullStr Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows
title_full_unstemmed Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows
title_sort Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows
dc.creator.none.fl_str_mv Minuti, Andrea
Zhou, Zheng
Graugnard, Daniel E
Rodriguez Zas, Sandra L.
Palladino, Rafael Alejandro
Cardoso, Felipe C.
Trevisi, Erminio
Loor, Juan J
author Minuti, Andrea
author_facet Minuti, Andrea
Zhou, Zheng
Graugnard, Daniel E
Rodriguez Zas, Sandra L.
Palladino, Rafael Alejandro
Cardoso, Felipe C.
Trevisi, Erminio
Loor, Juan J
author_role author
author2 Zhou, Zheng
Graugnard, Daniel E
Rodriguez Zas, Sandra L.
Palladino, Rafael Alejandro
Cardoso, Felipe C.
Trevisi, Erminio
Loor, Juan J
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MAMMARY
LIVER
LPS
EARLY LACTATION COWS
topic MAMMARY
LIVER
LPS
EARLY LACTATION COWS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The study investigated the effect of an intramammary lipopolysaccharide (LPS) challenge on the bovine mammary and liver transcriptome and its consequences on metabolic biomarkers and liver tissue composition. At 7 days of lactation, 7 cows served as controls (CTR) and 7 cows (LPS) received an intramammary Escherichia coli LPS challenge. The mammary and liver tissues for transcriptomic profiling were biopsied at 2.5 h from challenge. Liver composition was evaluated at 2.5 h and 7 days after challenge, and blood biomarkers were analyzed at 2, 3, 7 and 14 days from challenge. In mammary tissue, the LPS challenge resulted in 189 differentially expressed genes (DEG), with 20 down-regulated and 169 up-regulated. In liver tissue, there were 107 DEG in LPS compared with CTR with 42 down-regulated and 65 up-regulated. In mammary, bioinformatics analysis highlighted that LPS led to activation of NOD-like receptor signaling, Toll-like receptor signaling, RIG-I-like receptor signaling and apoptosis pathways. In liver, LPS resulted in an overall inhibition of fatty acid elongation in mitochondria and activation of the p53 signaling pathway. The LPS challenge induced changes in liver lipid composition, a systemic inflammation (rise of blood ceruloplasmin and bilirubin), and an increase in body fat mobilization. The data suggest that cells within the inflamed mammary gland respond by activating mechanisms of pathogen recognition. However, in the liver the response likely depends on mediators originating from the udder that affect liver functionality and specifically fatty acid metabolism (b-oxidation, ketogenesis, and lipoprotein synthesis).
Fil: Minuti, Andrea. Universita Cattolica del Sacro Cuore; Italia
Fil: Zhou, Zheng. University Of Illinois At Urbana; Estados Unidos
Fil: Graugnard, Daniel E. University Of Illinois At Urbana; Estados Unidos
Fil: Rodriguez Zas, Sandra L.. University Of Illinois At Urbana; Estados Unidos
Fil: Palladino, Rafael Alejandro. Universidad de Buenos Aires. Facultad de Agronomia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardoso, Felipe C.. University Of Illinois At Urbana; Estados Unidos
Fil: Trevisi, Erminio. Universita Cattolica del Sacro Cuore; Italia
Fil: Loor, Juan J. University Of Illinois At Urbana; Estados Unidos
description The study investigated the effect of an intramammary lipopolysaccharide (LPS) challenge on the bovine mammary and liver transcriptome and its consequences on metabolic biomarkers and liver tissue composition. At 7 days of lactation, 7 cows served as controls (CTR) and 7 cows (LPS) received an intramammary Escherichia coli LPS challenge. The mammary and liver tissues for transcriptomic profiling were biopsied at 2.5 h from challenge. Liver composition was evaluated at 2.5 h and 7 days after challenge, and blood biomarkers were analyzed at 2, 3, 7 and 14 days from challenge. In mammary tissue, the LPS challenge resulted in 189 differentially expressed genes (DEG), with 20 down-regulated and 169 up-regulated. In liver tissue, there were 107 DEG in LPS compared with CTR with 42 down-regulated and 65 up-regulated. In mammary, bioinformatics analysis highlighted that LPS led to activation of NOD-like receptor signaling, Toll-like receptor signaling, RIG-I-like receptor signaling and apoptosis pathways. In liver, LPS resulted in an overall inhibition of fatty acid elongation in mitochondria and activation of the p53 signaling pathway. The LPS challenge induced changes in liver lipid composition, a systemic inflammation (rise of blood ceruloplasmin and bilirubin), and an increase in body fat mobilization. The data suggest that cells within the inflamed mammary gland respond by activating mechanisms of pathogen recognition. However, in the liver the response likely depends on mediators originating from the udder that affect liver functionality and specifically fatty acid metabolism (b-oxidation, ketogenesis, and lipoprotein synthesis).
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16818
Minuti, Andrea; Zhou, Zheng; Graugnard, Daniel E; Rodriguez Zas, Sandra L.; Palladino, Rafael Alejandro; et al.; Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows; American Physiological Society; Physiological Reports; 3; 4; 4-2015; 1-12; e12388
2051-817X
url http://hdl.handle.net/11336/16818
identifier_str_mv Minuti, Andrea; Zhou, Zheng; Graugnard, Daniel E; Rodriguez Zas, Sandra L.; Palladino, Rafael Alejandro; et al.; Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows; American Physiological Society; Physiological Reports; 3; 4; 4-2015; 1-12; e12388
2051-817X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.14814/phy2.12388
info:eu-repo/semantics/altIdentifier/url/http://physreports.physiology.org/content/3/4/e12388
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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