The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormones

Autores
Varas, Silvia Mabel; Jahn, Graciela Alma
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to examine, using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) the changes in mRNA expression of the two estrogen receptor (ER) subtypes, ERα and ERβ, prolactin receptor long and short form, and progesterone (Pg) receptor (PgR), in liver and mammary gland during gestation, early lactation, and weaning in both hyperthyroid (HT) and normal rats. Pregnancy increased long prolactin receptors (PRL-RL) and ERα mRNAs in liver and PRL-RL in mammary gland. Lactation decreased PRL-RL in liver and ERβ and PgR in mammary gland. HT decreased PRL-RL at the end of pregnancy (G21), ERα (in G21 and L1) in liver and PRL-RL in L1 as well as short prolactin receptors (PRL-RS) (G7, L1) and ERβ (G7, G14, L4) in mammary gland. In conclusion, our data indicated that 1 PRL-R1 and ERα expression levels are differentially regulated in the liver, and PgR and ERβ in mammary gland during pregnancy and lactation 2 ERβ is variably expressed depending on the state of thyroid hormones, however the ERα gene expression remained constant in mammary gland. 3 PRL-R1 mRNA expression is highly induced in the mammary gland during late pregnancy and abruptly declines on the first day of lactation for the HT rats.
Fil: Varas, Silvia Mabel. Universidad Nacional de San Luis; Argentina
Fil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
L-THYROXINE
LACTATION
LIVER
MAMMARY GLAND
PREGNANCY
RT-PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163920
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/163920
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormonesVaras, Silvia MabelJahn, Graciela AlmaL-THYROXINELACTATIONLIVERMAMMARY GLANDPREGNANCYRT-PCRhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The aim of this study was to examine, using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) the changes in mRNA expression of the two estrogen receptor (ER) subtypes, ERα and ERβ, prolactin receptor long and short form, and progesterone (Pg) receptor (PgR), in liver and mammary gland during gestation, early lactation, and weaning in both hyperthyroid (HT) and normal rats. Pregnancy increased long prolactin receptors (PRL-RL) and ERα mRNAs in liver and PRL-RL in mammary gland. Lactation decreased PRL-RL in liver and ERβ and PgR in mammary gland. HT decreased PRL-RL at the end of pregnancy (G21), ERα (in G21 and L1) in liver and PRL-RL in L1 as well as short prolactin receptors (PRL-RS) (G7, L1) and ERβ (G7, G14, L4) in mammary gland. In conclusion, our data indicated that 1 PRL-R1 and ERα expression levels are differentially regulated in the liver, and PgR and ERβ in mammary gland during pregnancy and lactation 2 ERβ is variably expressed depending on the state of thyroid hormones, however the ERα gene expression remained constant in mammary gland. 3 PRL-R1 mRNA expression is highly induced in the mammary gland during late pregnancy and abruptly declines on the first day of lactation for the HT rats.Fil: Varas, Silvia Mabel. Universidad Nacional de San Luis; ArgentinaFil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaTaylor & Francis2005-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163920Varas, Silvia Mabel; Jahn, Graciela Alma; The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormones; Taylor & Francis; Endocrine Research; 31; 4; 12-2005; 357-3700743-58001532-4206CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/07435800500454528info:eu-repo/semantics/altIdentifier/doi/10.1080/07435800500454528info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:00:36Zoai:ri.conicet.gov.ar:11336/163920instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:00:36.243CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormones
title The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormones
spellingShingle The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormones
Varas, Silvia Mabel
L-THYROXINE
LACTATION
LIVER
MAMMARY GLAND
PREGNANCY
RT-PCR
title_short The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormones
title_full The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormones
title_fullStr The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormones
title_full_unstemmed The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormones
title_sort The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormones
dc.creator.none.fl_str_mv Varas, Silvia Mabel
Jahn, Graciela Alma
author Varas, Silvia Mabel
author_facet Varas, Silvia Mabel
Jahn, Graciela Alma
author_role author
author2 Jahn, Graciela Alma
author2_role author
dc.subject.none.fl_str_mv L-THYROXINE
LACTATION
LIVER
MAMMARY GLAND
PREGNANCY
RT-PCR
topic L-THYROXINE
LACTATION
LIVER
MAMMARY GLAND
PREGNANCY
RT-PCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this study was to examine, using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) the changes in mRNA expression of the two estrogen receptor (ER) subtypes, ERα and ERβ, prolactin receptor long and short form, and progesterone (Pg) receptor (PgR), in liver and mammary gland during gestation, early lactation, and weaning in both hyperthyroid (HT) and normal rats. Pregnancy increased long prolactin receptors (PRL-RL) and ERα mRNAs in liver and PRL-RL in mammary gland. Lactation decreased PRL-RL in liver and ERβ and PgR in mammary gland. HT decreased PRL-RL at the end of pregnancy (G21), ERα (in G21 and L1) in liver and PRL-RL in L1 as well as short prolactin receptors (PRL-RS) (G7, L1) and ERβ (G7, G14, L4) in mammary gland. In conclusion, our data indicated that 1 PRL-R1 and ERα expression levels are differentially regulated in the liver, and PgR and ERβ in mammary gland during pregnancy and lactation 2 ERβ is variably expressed depending on the state of thyroid hormones, however the ERα gene expression remained constant in mammary gland. 3 PRL-R1 mRNA expression is highly induced in the mammary gland during late pregnancy and abruptly declines on the first day of lactation for the HT rats.
Fil: Varas, Silvia Mabel. Universidad Nacional de San Luis; Argentina
Fil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description The aim of this study was to examine, using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) the changes in mRNA expression of the two estrogen receptor (ER) subtypes, ERα and ERβ, prolactin receptor long and short form, and progesterone (Pg) receptor (PgR), in liver and mammary gland during gestation, early lactation, and weaning in both hyperthyroid (HT) and normal rats. Pregnancy increased long prolactin receptors (PRL-RL) and ERα mRNAs in liver and PRL-RL in mammary gland. Lactation decreased PRL-RL in liver and ERβ and PgR in mammary gland. HT decreased PRL-RL at the end of pregnancy (G21), ERα (in G21 and L1) in liver and PRL-RL in L1 as well as short prolactin receptors (PRL-RS) (G7, L1) and ERβ (G7, G14, L4) in mammary gland. In conclusion, our data indicated that 1 PRL-R1 and ERα expression levels are differentially regulated in the liver, and PgR and ERβ in mammary gland during pregnancy and lactation 2 ERβ is variably expressed depending on the state of thyroid hormones, however the ERα gene expression remained constant in mammary gland. 3 PRL-R1 mRNA expression is highly induced in the mammary gland during late pregnancy and abruptly declines on the first day of lactation for the HT rats.
publishDate 2005
dc.date.none.fl_str_mv 2005-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163920
Varas, Silvia Mabel; Jahn, Graciela Alma; The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormones; Taylor & Francis; Endocrine Research; 31; 4; 12-2005; 357-370
0743-5800
1532-4206
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163920
identifier_str_mv Varas, Silvia Mabel; Jahn, Graciela Alma; The expression of estrogen, prolactin, and progesterone receptors in mammary gland and liver of female rats during pregnancy and early postpartum: Regulation by thyroid hormones; Taylor & Francis; Endocrine Research; 31; 4; 12-2005; 357-370
0743-5800
1532-4206
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/07435800500454528
info:eu-repo/semantics/altIdentifier/doi/10.1080/07435800500454528
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782333199319040
score 12.982451

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