The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeasts

Autores
Baker, Emily Clare; Wang, Bing; Bellora, Nicolás; Peris, David; Hulfachor, Amanda Beth; Koshalek, Justin A.; Adams, Marie; Libkind Frati, Diego; Hittinger, Chris Todd
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The dramatic phenotypic changes that occur in organisms during domestication leave indelible imprints on their genomes. Although many domesticated plants and animals have been systematically compared with their wild genetic stocks, the molecular and genomic processes underlying fungal domestication have received less attention. Here, we present a nearly complete genome assembly for the recently described yeast species Saccharomyces eubayanus and compare it to the genomes of multiple domesticated alloploid hybrids of S. eubayanus × S. cerevisiae (S. pastorianus syn. S. carlsbergensis), which are used to brew lager-style beers. We find that the S. eubayanus subgenomes of lager-brewing yeasts have experienced increased rates of evolution since hybridization, and that certain genes involved in metabolism may have been particularly affected. Interestingly, the S. eubayanus subgenome underwent an especially strong shift in selection regimes, consistent with more extensive domestication of the S. cerevisiae parent prior to hybridization. In contrast to recent proposals that lager-brewing yeasts were domesticated following a single hybridization event, the radically different neutral site divergences between the subgenomes of the two major lager yeast lineages strongly favor at least two independent origins for the S. cerevisiae × S. eubayanus hybrids that brew lager beers. Our findings demonstrate how this industrially important hybrid has been domesticated along similar evolutionary trajectories on multiple occasions.
Fil: Baker, Emily Clare. University Of Wisconsin; Estados Unidos
Fil: Wang, Bing. University Of Wisconsin; Estados Unidos
Fil: Bellora, Nicolás. Universidad Nacional del Comahue. Centro Regional Universidad de Bariloche. Departamento de Biologia. Laboratorio de Microbiologia Aplicada y Biotecnologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina
Fil: Peris, David. University Of Wisconsin; Estados Unidos
Fil: Hulfachor, Amanda Beth. University Of Wisconsin; Estados Unidos
Fil: Koshalek, Justin A.. University Of Wisconsin; Estados Unidos
Fil: Adams, Marie. University Of Wisconsin; Estados Unidos
Fil: Libkind Frati, Diego. Universidad Nacional del Comahue. Centro Regional Universidad de Bariloche. Departamento de Biologia. Laboratorio de Microbiologia Aplicada y Biotecnologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina
Fil: Hittinger, Chris Todd. University Of Wisconsin; Estados Unidos
Materia
Domestication
Hybridization
Saccharomyces eubayanus
Lager brewing
Genome assembly
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12214
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/12214
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeastsBaker, Emily ClareWang, BingBellora, NicolásPeris, DavidHulfachor, Amanda BethKoshalek, Justin A.Adams, MarieLibkind Frati, DiegoHittinger, Chris ToddDomesticationHybridizationSaccharomyces eubayanusLager brewingGenome assemblyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The dramatic phenotypic changes that occur in organisms during domestication leave indelible imprints on their genomes. Although many domesticated plants and animals have been systematically compared with their wild genetic stocks, the molecular and genomic processes underlying fungal domestication have received less attention. Here, we present a nearly complete genome assembly for the recently described yeast species Saccharomyces eubayanus and compare it to the genomes of multiple domesticated alloploid hybrids of S. eubayanus × S. cerevisiae (S. pastorianus syn. S. carlsbergensis), which are used to brew lager-style beers. We find that the S. eubayanus subgenomes of lager-brewing yeasts have experienced increased rates of evolution since hybridization, and that certain genes involved in metabolism may have been particularly affected. Interestingly, the S. eubayanus subgenome underwent an especially strong shift in selection regimes, consistent with more extensive domestication of the S. cerevisiae parent prior to hybridization. In contrast to recent proposals that lager-brewing yeasts were domesticated following a single hybridization event, the radically different neutral site divergences between the subgenomes of the two major lager yeast lineages strongly favor at least two independent origins for the S. cerevisiae × S. eubayanus hybrids that brew lager beers. Our findings demonstrate how this industrially important hybrid has been domesticated along similar evolutionary trajectories on multiple occasions.Fil: Baker, Emily Clare. University Of Wisconsin; Estados UnidosFil: Wang, Bing. University Of Wisconsin; Estados UnidosFil: Bellora, Nicolás. Universidad Nacional del Comahue. Centro Regional Universidad de Bariloche. Departamento de Biologia. Laboratorio de Microbiologia Aplicada y Biotecnologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; ArgentinaFil: Peris, David. University Of Wisconsin; Estados UnidosFil: Hulfachor, Amanda Beth. University Of Wisconsin; Estados UnidosFil: Koshalek, Justin A.. University Of Wisconsin; Estados UnidosFil: Adams, Marie. University Of Wisconsin; Estados UnidosFil: Libkind Frati, Diego. Universidad Nacional del Comahue. Centro Regional Universidad de Bariloche. Departamento de Biologia. Laboratorio de Microbiologia Aplicada y Biotecnologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; ArgentinaFil: Hittinger, Chris Todd. University Of Wisconsin; Estados UnidosOxford University Press2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12214Baker, Emily Clare; Wang, Bing; Bellora, Nicolás; Peris, David; Hulfachor, Amanda Beth; et al.; The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeasts; Oxford University Press; Molecular Biology And Evolution; 32; 11; 8-2015; 2818-28310737-40381537-1719enginfo:eu-repo/semantics/altIdentifier/doi/10.1093/molbev/msv168info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mbe/article/32/11/2818/982141/The-Genome-Sequence-of-Saccharomyces-eubayanus-andinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:43Zoai:ri.conicet.gov.ar:11336/12214instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:44.096CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeasts
title The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeasts
spellingShingle The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeasts
Baker, Emily Clare
Domestication
Hybridization
Saccharomyces eubayanus
Lager brewing
Genome assembly
title_short The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeasts
title_full The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeasts
title_fullStr The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeasts
title_full_unstemmed The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeasts
title_sort The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeasts
dc.creator.none.fl_str_mv Baker, Emily Clare
Wang, Bing
Bellora, Nicolás
Peris, David
Hulfachor, Amanda Beth
Koshalek, Justin A.
Adams, Marie
Libkind Frati, Diego
Hittinger, Chris Todd
author Baker, Emily Clare
author_facet Baker, Emily Clare
Wang, Bing
Bellora, Nicolás
Peris, David
Hulfachor, Amanda Beth
Koshalek, Justin A.
Adams, Marie
Libkind Frati, Diego
Hittinger, Chris Todd
author_role author
author2 Wang, Bing
Bellora, Nicolás
Peris, David
Hulfachor, Amanda Beth
Koshalek, Justin A.
Adams, Marie
Libkind Frati, Diego
Hittinger, Chris Todd
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Domestication
Hybridization
Saccharomyces eubayanus
Lager brewing
Genome assembly
topic Domestication
Hybridization
Saccharomyces eubayanus
Lager brewing
Genome assembly
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The dramatic phenotypic changes that occur in organisms during domestication leave indelible imprints on their genomes. Although many domesticated plants and animals have been systematically compared with their wild genetic stocks, the molecular and genomic processes underlying fungal domestication have received less attention. Here, we present a nearly complete genome assembly for the recently described yeast species Saccharomyces eubayanus and compare it to the genomes of multiple domesticated alloploid hybrids of S. eubayanus × S. cerevisiae (S. pastorianus syn. S. carlsbergensis), which are used to brew lager-style beers. We find that the S. eubayanus subgenomes of lager-brewing yeasts have experienced increased rates of evolution since hybridization, and that certain genes involved in metabolism may have been particularly affected. Interestingly, the S. eubayanus subgenome underwent an especially strong shift in selection regimes, consistent with more extensive domestication of the S. cerevisiae parent prior to hybridization. In contrast to recent proposals that lager-brewing yeasts were domesticated following a single hybridization event, the radically different neutral site divergences between the subgenomes of the two major lager yeast lineages strongly favor at least two independent origins for the S. cerevisiae × S. eubayanus hybrids that brew lager beers. Our findings demonstrate how this industrially important hybrid has been domesticated along similar evolutionary trajectories on multiple occasions.
Fil: Baker, Emily Clare. University Of Wisconsin; Estados Unidos
Fil: Wang, Bing. University Of Wisconsin; Estados Unidos
Fil: Bellora, Nicolás. Universidad Nacional del Comahue. Centro Regional Universidad de Bariloche. Departamento de Biologia. Laboratorio de Microbiologia Aplicada y Biotecnologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina
Fil: Peris, David. University Of Wisconsin; Estados Unidos
Fil: Hulfachor, Amanda Beth. University Of Wisconsin; Estados Unidos
Fil: Koshalek, Justin A.. University Of Wisconsin; Estados Unidos
Fil: Adams, Marie. University Of Wisconsin; Estados Unidos
Fil: Libkind Frati, Diego. Universidad Nacional del Comahue. Centro Regional Universidad de Bariloche. Departamento de Biologia. Laboratorio de Microbiologia Aplicada y Biotecnologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina
Fil: Hittinger, Chris Todd. University Of Wisconsin; Estados Unidos
description The dramatic phenotypic changes that occur in organisms during domestication leave indelible imprints on their genomes. Although many domesticated plants and animals have been systematically compared with their wild genetic stocks, the molecular and genomic processes underlying fungal domestication have received less attention. Here, we present a nearly complete genome assembly for the recently described yeast species Saccharomyces eubayanus and compare it to the genomes of multiple domesticated alloploid hybrids of S. eubayanus × S. cerevisiae (S. pastorianus syn. S. carlsbergensis), which are used to brew lager-style beers. We find that the S. eubayanus subgenomes of lager-brewing yeasts have experienced increased rates of evolution since hybridization, and that certain genes involved in metabolism may have been particularly affected. Interestingly, the S. eubayanus subgenome underwent an especially strong shift in selection regimes, consistent with more extensive domestication of the S. cerevisiae parent prior to hybridization. In contrast to recent proposals that lager-brewing yeasts were domesticated following a single hybridization event, the radically different neutral site divergences between the subgenomes of the two major lager yeast lineages strongly favor at least two independent origins for the S. cerevisiae × S. eubayanus hybrids that brew lager beers. Our findings demonstrate how this industrially important hybrid has been domesticated along similar evolutionary trajectories on multiple occasions.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12214
Baker, Emily Clare; Wang, Bing; Bellora, Nicolás; Peris, David; Hulfachor, Amanda Beth; et al.; The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeasts; Oxford University Press; Molecular Biology And Evolution; 32; 11; 8-2015; 2818-2831
0737-4038
1537-1719
url http://hdl.handle.net/11336/12214
identifier_str_mv Baker, Emily Clare; Wang, Bing; Bellora, Nicolás; Peris, David; Hulfachor, Amanda Beth; et al.; The genome sequence of Saccharomyces Eubayanus and the domestication of Lager-Brewing yeasts; Oxford University Press; Molecular Biology And Evolution; 32; 11; 8-2015; 2818-2831
0737-4038
1537-1719
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/molbev/msv168
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mbe/article/32/11/2818/982141/The-Genome-Sequence-of-Saccharomyces-eubayanus-and
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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