Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains

Autores
Molinet, Jennifer; Eizaguirre, Juan Ignacio; Quintrel, Pablo; Bellora, Nicolás; Villarroel, Carlos A.; Villarreal, Pablo; Benavides Parra, José; Nespolo, Roberto F.; Libkind Frati, Diego; Cubillos, Francisco A.
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The study of natural variation can untap novel alleles with immense value for biotechnological applications. Saccharomyces eubayanus Patagonian isolates exhibit differences in the diauxic shift between glucose and maltose, representing a suitable model to study their natural genetic variation for novel strains for brewing. However, little is known about the genetic variants and chromatin regulators responsible for these differences. Here, we show how genome-wide chromatin accessibility and gene expression differences underlie distinct diauxic shift profiles in S. eubayanus. We identified two strains with a rapid diauxic shift between glucose and maltose (CL467.1 and CBS12357) and one strain with a remarkably low fermentation efficiency and longer lag phase during diauxic shift (QC18). This is associated in the QC18 strain with lower transcriptional activity and chromatin accessibility of specific genes of maltose metabolism and higher expression levels of glucose transporters. These differences are governed by the HAP complex, which differentially regulates gene expression depending on the genetic background. We found in the QC18 strain a contrasting phenotype to those phenotypes described in S. cerevisiae, where hap4D, hap5D, and cin5D knockouts significantly improved the QC18 growth rate in the glucose-maltose shift. The most profound effects were found between CIN5 allelic variants, suggesting that Cin5p could strongly activate a repressor of the diauxic shift in the QC18 strain but not necessarily in the other strains. The differences between strains could originate from the tree host from which the strains were obtained, which might determine the sugar source preference and the brewing potential of the strain.
Fil: Molinet, Jennifer. Universidad de Santiago de Chile; Chile
Fil: Eizaguirre, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Quintrel, Pablo. Universidad de Santiago de Chile; Chile
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Instituto de Tecnologías Nucleares para la Salud; Argentina
Fil: Villarroel, Carlos A.. Universidad de Talca; Chile
Fil: Villarreal, Pablo. Universidad de Santiago de Chile; Chile
Fil: Benavides Parra, José. Universidad de Santiago de Chile; Chile
Fil: Nespolo, Roberto F.. Universidad Austral de Chile; Chile
Fil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Cubillos, Francisco A.. Universidad de Santiago de Chile; Chile
Materia
Diauxic Shift
Saccharomyces eubayanus
Patagonia
Lager beer
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214241
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/214241
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus StrainsMolinet, JenniferEizaguirre, Juan IgnacioQuintrel, PabloBellora, NicolásVillarroel, Carlos A.Villarreal, PabloBenavides Parra, JoséNespolo, Roberto F.Libkind Frati, DiegoCubillos, Francisco A.Diauxic ShiftSaccharomyces eubayanusPatagoniaLager beerhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The study of natural variation can untap novel alleles with immense value for biotechnological applications. Saccharomyces eubayanus Patagonian isolates exhibit differences in the diauxic shift between glucose and maltose, representing a suitable model to study their natural genetic variation for novel strains for brewing. However, little is known about the genetic variants and chromatin regulators responsible for these differences. Here, we show how genome-wide chromatin accessibility and gene expression differences underlie distinct diauxic shift profiles in S. eubayanus. We identified two strains with a rapid diauxic shift between glucose and maltose (CL467.1 and CBS12357) and one strain with a remarkably low fermentation efficiency and longer lag phase during diauxic shift (QC18). This is associated in the QC18 strain with lower transcriptional activity and chromatin accessibility of specific genes of maltose metabolism and higher expression levels of glucose transporters. These differences are governed by the HAP complex, which differentially regulates gene expression depending on the genetic background. We found in the QC18 strain a contrasting phenotype to those phenotypes described in S. cerevisiae, where hap4D, hap5D, and cin5D knockouts significantly improved the QC18 growth rate in the glucose-maltose shift. The most profound effects were found between CIN5 allelic variants, suggesting that Cin5p could strongly activate a repressor of the diauxic shift in the QC18 strain but not necessarily in the other strains. The differences between strains could originate from the tree host from which the strains were obtained, which might determine the sugar source preference and the brewing potential of the strain.Fil: Molinet, Jennifer. Universidad de Santiago de Chile; ChileFil: Eizaguirre, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Quintrel, Pablo. Universidad de Santiago de Chile; ChileFil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Instituto de Tecnologías Nucleares para la Salud; ArgentinaFil: Villarroel, Carlos A.. Universidad de Talca; ChileFil: Villarreal, Pablo. Universidad de Santiago de Chile; ChileFil: Benavides Parra, José. Universidad de Santiago de Chile; ChileFil: Nespolo, Roberto F.. Universidad Austral de Chile; ChileFil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Cubillos, Francisco A.. Universidad de Santiago de Chile; ChileAmerican Society for Microbiology2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214241Molinet, Jennifer; Eizaguirre, Juan Ignacio; Quintrel, Pablo; Bellora, Nicolás; Villarroel, Carlos A.; et al.; Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains; American Society for Microbiology; mSystems; 7; 6; 12-2022; 1-182379-5077CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/msystems.00640-22info:eu-repo/semantics/altIdentifier/doi/10.1128/msystems.00640-22info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:37Zoai:ri.conicet.gov.ar:11336/214241instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:37.232CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains
title Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains
spellingShingle Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains
Molinet, Jennifer
Diauxic Shift
Saccharomyces eubayanus
Patagonia
Lager beer
title_short Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains
title_full Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains
title_fullStr Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains
title_full_unstemmed Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains
title_sort Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains
dc.creator.none.fl_str_mv Molinet, Jennifer
Eizaguirre, Juan Ignacio
Quintrel, Pablo
Bellora, Nicolás
Villarroel, Carlos A.
Villarreal, Pablo
Benavides Parra, José
Nespolo, Roberto F.
Libkind Frati, Diego
Cubillos, Francisco A.
author Molinet, Jennifer
author_facet Molinet, Jennifer
Eizaguirre, Juan Ignacio
Quintrel, Pablo
Bellora, Nicolás
Villarroel, Carlos A.
Villarreal, Pablo
Benavides Parra, José
Nespolo, Roberto F.
Libkind Frati, Diego
Cubillos, Francisco A.
author_role author
author2 Eizaguirre, Juan Ignacio
Quintrel, Pablo
Bellora, Nicolás
Villarroel, Carlos A.
Villarreal, Pablo
Benavides Parra, José
Nespolo, Roberto F.
Libkind Frati, Diego
Cubillos, Francisco A.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Diauxic Shift
Saccharomyces eubayanus
Patagonia
Lager beer
topic Diauxic Shift
Saccharomyces eubayanus
Patagonia
Lager beer
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The study of natural variation can untap novel alleles with immense value for biotechnological applications. Saccharomyces eubayanus Patagonian isolates exhibit differences in the diauxic shift between glucose and maltose, representing a suitable model to study their natural genetic variation for novel strains for brewing. However, little is known about the genetic variants and chromatin regulators responsible for these differences. Here, we show how genome-wide chromatin accessibility and gene expression differences underlie distinct diauxic shift profiles in S. eubayanus. We identified two strains with a rapid diauxic shift between glucose and maltose (CL467.1 and CBS12357) and one strain with a remarkably low fermentation efficiency and longer lag phase during diauxic shift (QC18). This is associated in the QC18 strain with lower transcriptional activity and chromatin accessibility of specific genes of maltose metabolism and higher expression levels of glucose transporters. These differences are governed by the HAP complex, which differentially regulates gene expression depending on the genetic background. We found in the QC18 strain a contrasting phenotype to those phenotypes described in S. cerevisiae, where hap4D, hap5D, and cin5D knockouts significantly improved the QC18 growth rate in the glucose-maltose shift. The most profound effects were found between CIN5 allelic variants, suggesting that Cin5p could strongly activate a repressor of the diauxic shift in the QC18 strain but not necessarily in the other strains. The differences between strains could originate from the tree host from which the strains were obtained, which might determine the sugar source preference and the brewing potential of the strain.
Fil: Molinet, Jennifer. Universidad de Santiago de Chile; Chile
Fil: Eizaguirre, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Quintrel, Pablo. Universidad de Santiago de Chile; Chile
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Instituto de Tecnologías Nucleares para la Salud; Argentina
Fil: Villarroel, Carlos A.. Universidad de Talca; Chile
Fil: Villarreal, Pablo. Universidad de Santiago de Chile; Chile
Fil: Benavides Parra, José. Universidad de Santiago de Chile; Chile
Fil: Nespolo, Roberto F.. Universidad Austral de Chile; Chile
Fil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Cubillos, Francisco A.. Universidad de Santiago de Chile; Chile
description The study of natural variation can untap novel alleles with immense value for biotechnological applications. Saccharomyces eubayanus Patagonian isolates exhibit differences in the diauxic shift between glucose and maltose, representing a suitable model to study their natural genetic variation for novel strains for brewing. However, little is known about the genetic variants and chromatin regulators responsible for these differences. Here, we show how genome-wide chromatin accessibility and gene expression differences underlie distinct diauxic shift profiles in S. eubayanus. We identified two strains with a rapid diauxic shift between glucose and maltose (CL467.1 and CBS12357) and one strain with a remarkably low fermentation efficiency and longer lag phase during diauxic shift (QC18). This is associated in the QC18 strain with lower transcriptional activity and chromatin accessibility of specific genes of maltose metabolism and higher expression levels of glucose transporters. These differences are governed by the HAP complex, which differentially regulates gene expression depending on the genetic background. We found in the QC18 strain a contrasting phenotype to those phenotypes described in S. cerevisiae, where hap4D, hap5D, and cin5D knockouts significantly improved the QC18 growth rate in the glucose-maltose shift. The most profound effects were found between CIN5 allelic variants, suggesting that Cin5p could strongly activate a repressor of the diauxic shift in the QC18 strain but not necessarily in the other strains. The differences between strains could originate from the tree host from which the strains were obtained, which might determine the sugar source preference and the brewing potential of the strain.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214241
Molinet, Jennifer; Eizaguirre, Juan Ignacio; Quintrel, Pablo; Bellora, Nicolás; Villarroel, Carlos A.; et al.; Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains; American Society for Microbiology; mSystems; 7; 6; 12-2022; 1-18
2379-5077
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214241
identifier_str_mv Molinet, Jennifer; Eizaguirre, Juan Ignacio; Quintrel, Pablo; Bellora, Nicolás; Villarroel, Carlos A.; et al.; Natural Variation in Diauxic Shift between Patagonian Saccharomyces eubayanus Strains; American Society for Microbiology; mSystems; 7; 6; 12-2022; 1-18
2379-5077
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/msystems.00640-22
info:eu-repo/semantics/altIdentifier/doi/10.1128/msystems.00640-22
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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