Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors

Autores
Grynberg, Michaël; Pierre, Alice; Rey, Rodolfo Alberto; Leclerc, Arnaud; Arouche, Nassim; Hesters, Laetitia; Catteau Jonard, Sophie; Frydman, René; Picard, Jean Yves; Fanchin, Renato; Veitia, Reiner; Di Clemente, Nathalie; Taieb, Joëlle
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Anti-Müllerian hormone (AMH) is a member of the TGF-β family, which limits follicle maturation. Recently serum AMH has been recognized as a useful diagnostic and prognostic tool in human reproductive endocrinology. Objective: The aim of this study was to investigate the regulation of human ovarian AMH by estradiol and FSH. Methods: AMH mRNA were quantified by real time RT-PCR in human granulosa cells (GC). AMH transcription was studied in KK1 GC cotransfected with estrogen receptors (ER)-β or ERα, and normal human AMH promoter-luciferase construct (hAMH-luc) or mutated AMH promoter reporter constructs. Binding sites for estradiol (estrogen response element half-site) and steroidogenic factor 1 were disrupted by targeted mutagenesis. The level of ER in GC was determined by quantitative RT-PCR and Western blotting. Results: In KK1 cells, estradiol up-regulated and inhibited hAMH-luc in the presence of ERα and ERβrespectively. Disruption of estrogen response element half-site and/or steroidogenic factor 1 binding sites did not modify ERβ-mediated effect of estradiol on hAMH-luc, whereas it affected that conveyed by ERα. The FSH enhancement of hAMH-luc was abolished by estradiol in cells overexpressing ERβ. When both ER were transfected, estradiol inhibited hAMH-luc or had no effect. Estradiol repressed AMH mRNAs in human GC, which express a little more ERα than ERβ mRNA. Conclusions: Our results show that AMH expression can be differentially regulated by estradiol depending on the ER and suggest that its decrease in GC of growing follicles, which mainly express ERβ, and during controlled ovarian hyperstimulation is due to the effect of estradiol.
Fil: Grynberg, Michaël. Inserm; Francia
Fil: Pierre, Alice. Inserm; Francia
Fil: Rey, Rodolfo Alberto. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leclerc, Arnaud. Inserm; Francia
Fil: Arouche, Nassim. Inserm; Francia
Fil: Hesters, Laetitia. Inserm; Francia
Fil: Catteau Jonard, Sophie. Universite Lille; Francia
Fil: Frydman, René. Inserm; Francia
Fil: Picard, Jean Yves. Inserm; Francia
Fil: Fanchin, Renato. Inserm; Francia
Fil: Veitia, Reiner. Centre National de la Recherche Scientifique; Francia
Fil: Di Clemente, Nathalie. Inserm; Francia
Fil: Taieb, Joëlle. Inserm; Francia
Materia
Anti-Müllerian Hormone
Ovary
Estrogen Receptor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/68131
Acceder
id CONICETDig_d87a40a4af822c2e1750f23b66638391
oai_identifier_str oai:ri.conicet.gov.ar:11336/68131
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptorsGrynberg, MichaëlPierre, AliceRey, Rodolfo AlbertoLeclerc, ArnaudArouche, NassimHesters, LaetitiaCatteau Jonard, SophieFrydman, RenéPicard, Jean YvesFanchin, RenatoVeitia, ReinerDi Clemente, NathalieTaieb, JoëlleAnti-Müllerian HormoneOvaryEstrogen Receptorhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Anti-Müllerian hormone (AMH) is a member of the TGF-β family, which limits follicle maturation. Recently serum AMH has been recognized as a useful diagnostic and prognostic tool in human reproductive endocrinology. Objective: The aim of this study was to investigate the regulation of human ovarian AMH by estradiol and FSH. Methods: AMH mRNA were quantified by real time RT-PCR in human granulosa cells (GC). AMH transcription was studied in KK1 GC cotransfected with estrogen receptors (ER)-β or ERα, and normal human AMH promoter-luciferase construct (hAMH-luc) or mutated AMH promoter reporter constructs. Binding sites for estradiol (estrogen response element half-site) and steroidogenic factor 1 were disrupted by targeted mutagenesis. The level of ER in GC was determined by quantitative RT-PCR and Western blotting. Results: In KK1 cells, estradiol up-regulated and inhibited hAMH-luc in the presence of ERα and ERβrespectively. Disruption of estrogen response element half-site and/or steroidogenic factor 1 binding sites did not modify ERβ-mediated effect of estradiol on hAMH-luc, whereas it affected that conveyed by ERα. The FSH enhancement of hAMH-luc was abolished by estradiol in cells overexpressing ERβ. When both ER were transfected, estradiol inhibited hAMH-luc or had no effect. Estradiol repressed AMH mRNAs in human GC, which express a little more ERα than ERβ mRNA. Conclusions: Our results show that AMH expression can be differentially regulated by estradiol depending on the ER and suggest that its decrease in GC of growing follicles, which mainly express ERβ, and during controlled ovarian hyperstimulation is due to the effect of estradiol.Fil: Grynberg, Michaël. Inserm; FranciaFil: Pierre, Alice. Inserm; FranciaFil: Rey, Rodolfo Alberto. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leclerc, Arnaud. Inserm; FranciaFil: Arouche, Nassim. Inserm; FranciaFil: Hesters, Laetitia. Inserm; FranciaFil: Catteau Jonard, Sophie. Universite Lille; FranciaFil: Frydman, René. Inserm; FranciaFil: Picard, Jean Yves. Inserm; FranciaFil: Fanchin, Renato. Inserm; FranciaFil: Veitia, Reiner. Centre National de la Recherche Scientifique; FranciaFil: Di Clemente, Nathalie. Inserm; FranciaFil: Taieb, Joëlle. Inserm; FranciaEndocrine Society2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68131Grynberg, Michaël; Pierre, Alice; Rey, Rodolfo Alberto; Leclerc, Arnaud; Arouche, Nassim; et al.; Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors; Endocrine Society; Journal of Clinical Endocrinology and Metabolism; 97; 9; 9-2012; 1649-16570021-972XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1210/jc.2011-3133info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jcem/article/97/9/E1649/2536530info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:31Zoai:ri.conicet.gov.ar:11336/68131instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:32.09CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors
title Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors
spellingShingle Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors
Grynberg, Michaël
Anti-Müllerian Hormone
Ovary
Estrogen Receptor
title_short Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors
title_full Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors
title_fullStr Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors
title_full_unstemmed Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors
title_sort Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors
dc.creator.none.fl_str_mv Grynberg, Michaël
Pierre, Alice
Rey, Rodolfo Alberto
Leclerc, Arnaud
Arouche, Nassim
Hesters, Laetitia
Catteau Jonard, Sophie
Frydman, René
Picard, Jean Yves
Fanchin, Renato
Veitia, Reiner
Di Clemente, Nathalie
Taieb, Joëlle
author Grynberg, Michaël
author_facet Grynberg, Michaël
Pierre, Alice
Rey, Rodolfo Alberto
Leclerc, Arnaud
Arouche, Nassim
Hesters, Laetitia
Catteau Jonard, Sophie
Frydman, René
Picard, Jean Yves
Fanchin, Renato
Veitia, Reiner
Di Clemente, Nathalie
Taieb, Joëlle
author_role author
author2 Pierre, Alice
Rey, Rodolfo Alberto
Leclerc, Arnaud
Arouche, Nassim
Hesters, Laetitia
Catteau Jonard, Sophie
Frydman, René
Picard, Jean Yves
Fanchin, Renato
Veitia, Reiner
Di Clemente, Nathalie
Taieb, Joëlle
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anti-Müllerian Hormone
Ovary
Estrogen Receptor
topic Anti-Müllerian Hormone
Ovary
Estrogen Receptor
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Anti-Müllerian hormone (AMH) is a member of the TGF-β family, which limits follicle maturation. Recently serum AMH has been recognized as a useful diagnostic and prognostic tool in human reproductive endocrinology. Objective: The aim of this study was to investigate the regulation of human ovarian AMH by estradiol and FSH. Methods: AMH mRNA were quantified by real time RT-PCR in human granulosa cells (GC). AMH transcription was studied in KK1 GC cotransfected with estrogen receptors (ER)-β or ERα, and normal human AMH promoter-luciferase construct (hAMH-luc) or mutated AMH promoter reporter constructs. Binding sites for estradiol (estrogen response element half-site) and steroidogenic factor 1 were disrupted by targeted mutagenesis. The level of ER in GC was determined by quantitative RT-PCR and Western blotting. Results: In KK1 cells, estradiol up-regulated and inhibited hAMH-luc in the presence of ERα and ERβrespectively. Disruption of estrogen response element half-site and/or steroidogenic factor 1 binding sites did not modify ERβ-mediated effect of estradiol on hAMH-luc, whereas it affected that conveyed by ERα. The FSH enhancement of hAMH-luc was abolished by estradiol in cells overexpressing ERβ. When both ER were transfected, estradiol inhibited hAMH-luc or had no effect. Estradiol repressed AMH mRNAs in human GC, which express a little more ERα than ERβ mRNA. Conclusions: Our results show that AMH expression can be differentially regulated by estradiol depending on the ER and suggest that its decrease in GC of growing follicles, which mainly express ERβ, and during controlled ovarian hyperstimulation is due to the effect of estradiol.
Fil: Grynberg, Michaël. Inserm; Francia
Fil: Pierre, Alice. Inserm; Francia
Fil: Rey, Rodolfo Alberto. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leclerc, Arnaud. Inserm; Francia
Fil: Arouche, Nassim. Inserm; Francia
Fil: Hesters, Laetitia. Inserm; Francia
Fil: Catteau Jonard, Sophie. Universite Lille; Francia
Fil: Frydman, René. Inserm; Francia
Fil: Picard, Jean Yves. Inserm; Francia
Fil: Fanchin, Renato. Inserm; Francia
Fil: Veitia, Reiner. Centre National de la Recherche Scientifique; Francia
Fil: Di Clemente, Nathalie. Inserm; Francia
Fil: Taieb, Joëlle. Inserm; Francia
description Background: Anti-Müllerian hormone (AMH) is a member of the TGF-β family, which limits follicle maturation. Recently serum AMH has been recognized as a useful diagnostic and prognostic tool in human reproductive endocrinology. Objective: The aim of this study was to investigate the regulation of human ovarian AMH by estradiol and FSH. Methods: AMH mRNA were quantified by real time RT-PCR in human granulosa cells (GC). AMH transcription was studied in KK1 GC cotransfected with estrogen receptors (ER)-β or ERα, and normal human AMH promoter-luciferase construct (hAMH-luc) or mutated AMH promoter reporter constructs. Binding sites for estradiol (estrogen response element half-site) and steroidogenic factor 1 were disrupted by targeted mutagenesis. The level of ER in GC was determined by quantitative RT-PCR and Western blotting. Results: In KK1 cells, estradiol up-regulated and inhibited hAMH-luc in the presence of ERα and ERβrespectively. Disruption of estrogen response element half-site and/or steroidogenic factor 1 binding sites did not modify ERβ-mediated effect of estradiol on hAMH-luc, whereas it affected that conveyed by ERα. The FSH enhancement of hAMH-luc was abolished by estradiol in cells overexpressing ERβ. When both ER were transfected, estradiol inhibited hAMH-luc or had no effect. Estradiol repressed AMH mRNAs in human GC, which express a little more ERα than ERβ mRNA. Conclusions: Our results show that AMH expression can be differentially regulated by estradiol depending on the ER and suggest that its decrease in GC of growing follicles, which mainly express ERβ, and during controlled ovarian hyperstimulation is due to the effect of estradiol.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68131
Grynberg, Michaël; Pierre, Alice; Rey, Rodolfo Alberto; Leclerc, Arnaud; Arouche, Nassim; et al.; Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors; Endocrine Society; Journal of Clinical Endocrinology and Metabolism; 97; 9; 9-2012; 1649-1657
0021-972X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68131
identifier_str_mv Grynberg, Michaël; Pierre, Alice; Rey, Rodolfo Alberto; Leclerc, Arnaud; Arouche, Nassim; et al.; Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors; Endocrine Society; Journal of Clinical Endocrinology and Metabolism; 97; 9; 9-2012; 1649-1657
0021-972X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1210/jc.2011-3133
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jcem/article/97/9/E1649/2536530
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Endocrine Society
publisher.none.fl_str_mv Endocrine Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269291021336576
score 13.13397

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