Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male rats
- Autores
- Wilda, Maximiliano; Grigera, Pablo Rafael
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Polysaccharide microparticles (PSMs) are a promising carrier system for the oral administration of antigens. They can protect antigens from degradation in the harsh environment of the gut. They can also improve the uptake of antigens by the immune system. Anti-GnRH vaccines work by blocking the binding of GnRH to its receptors on the cells of the hypothalamus and pituitary gland, preventing the release of LH and FSH and consequently suppress sex hormone production. Anti-GnRH injectable vaccines have been developed for a variety of production animals as an alternative to surgical castration. In this work we orally delivered polysaccharide microparticles containing a recombinant fusion protein as an antigen to develop Anti-GnRH response in male rat. We designed and expressed a recombinant 45 KDa peptide (GF45) consisting of a 4 tandem repeats from the GnRH sequence EHWSYGLRPG fused to an immunogenic carrier peptide which contains rickettsial Hsp70 and Hsp60 sequences. GF was encapsulated in alginate-chitosan particles by ionic reverse gelation. Particles containing 200 ug of recombinant peptide were orally administrated to a group of 4-week year old male Sprague-Dowley rats (n=10) in a prime-boost immunization scheme. Empty particles and injectable GF were used as controls. Serum testosterone and anti-GF45 antibodies were analysed by ELISA method along with the weight and volume of testicles. The GF45 orally treated group showed detectable titters of anti-GF45 antibodies and a 95% reduction of circulating testosterone in comparison to the control group. Testicles in treated group showed a reduction of 52% in weight and 50% in volume regarding to control. The encapsulation and oral delivery of the recombinant GF45 in alginate-chitosan microparticles generated autoimmune response capable of blocking the function of endogen GnRH. These particles are a suitable not only for generating non injectable or surgical control of GnRH but also to possibly deliver other recombinant antigens.
Fil: Wilda, Maximiliano. Ministerio de Produccion y Trabajo. Secretaria de Gobierno de Agroindustria. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Centro de Virologia Animal. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Virologia Animal.; Argentina
Fil: Grigera, Pablo Rafael. Ministerio de Produccion y Trabajo. Secretaria de Gobierno de Agroindustria. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Centro de Virologia Animal. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Virologia Animal.; Argentina
13th International Veterinary Immunology Symposium
Mpumalanga
Sudáfrica
International Union of Immunological Societies - Materia
-
microparticulas
vacunas orales
inmunogeno oral
GnRH - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/249500
Ver los metadatos del registro completo
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Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male ratsWilda, MaximilianoGrigera, Pablo Rafaelmicroparticulasvacunas oralesinmunogeno oralGnRHhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Polysaccharide microparticles (PSMs) are a promising carrier system for the oral administration of antigens. They can protect antigens from degradation in the harsh environment of the gut. They can also improve the uptake of antigens by the immune system. Anti-GnRH vaccines work by blocking the binding of GnRH to its receptors on the cells of the hypothalamus and pituitary gland, preventing the release of LH and FSH and consequently suppress sex hormone production. Anti-GnRH injectable vaccines have been developed for a variety of production animals as an alternative to surgical castration. In this work we orally delivered polysaccharide microparticles containing a recombinant fusion protein as an antigen to develop Anti-GnRH response in male rat. We designed and expressed a recombinant 45 KDa peptide (GF45) consisting of a 4 tandem repeats from the GnRH sequence EHWSYGLRPG fused to an immunogenic carrier peptide which contains rickettsial Hsp70 and Hsp60 sequences. GF was encapsulated in alginate-chitosan particles by ionic reverse gelation. Particles containing 200 ug of recombinant peptide were orally administrated to a group of 4-week year old male Sprague-Dowley rats (n=10) in a prime-boost immunization scheme. Empty particles and injectable GF were used as controls. Serum testosterone and anti-GF45 antibodies were analysed by ELISA method along with the weight and volume of testicles. The GF45 orally treated group showed detectable titters of anti-GF45 antibodies and a 95% reduction of circulating testosterone in comparison to the control group. Testicles in treated group showed a reduction of 52% in weight and 50% in volume regarding to control. The encapsulation and oral delivery of the recombinant GF45 in alginate-chitosan microparticles generated autoimmune response capable of blocking the function of endogen GnRH. These particles are a suitable not only for generating non injectable or surgical control of GnRH but also to possibly deliver other recombinant antigens.Fil: Wilda, Maximiliano. Ministerio de Produccion y Trabajo. Secretaria de Gobierno de Agroindustria. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Centro de Virologia Animal. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Virologia Animal.; ArgentinaFil: Grigera, Pablo Rafael. Ministerio de Produccion y Trabajo. Secretaria de Gobierno de Agroindustria. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Centro de Virologia Animal. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Virologia Animal.; Argentina13th International Veterinary Immunology SymposiumMpumalangaSudáfricaInternational Union of Immunological SocietiesInternational Union of Immunological Societies2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/249500Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male rats; 13th International Veterinary Immunology Symposium; Mpumalanga; Sudáfrica; 2023; 1-3CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ivis2023.org/wp-content/uploads/2024/05/IVIS2023-Book-of-Abstracts_17-November-1.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:01:52Zoai:ri.conicet.gov.ar:11336/249500instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:01:52.802CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male rats |
| title |
Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male rats |
| spellingShingle |
Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male rats Wilda, Maximiliano microparticulas vacunas orales inmunogeno oral GnRH |
| title_short |
Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male rats |
| title_full |
Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male rats |
| title_fullStr |
Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male rats |
| title_full_unstemmed |
Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male rats |
| title_sort |
Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male rats |
| dc.creator.none.fl_str_mv |
Wilda, Maximiliano Grigera, Pablo Rafael |
| author |
Wilda, Maximiliano |
| author_facet |
Wilda, Maximiliano Grigera, Pablo Rafael |
| author_role |
author |
| author2 |
Grigera, Pablo Rafael |
| author2_role |
author |
| dc.subject.none.fl_str_mv |
microparticulas vacunas orales inmunogeno oral GnRH |
| topic |
microparticulas vacunas orales inmunogeno oral GnRH |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.7 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Polysaccharide microparticles (PSMs) are a promising carrier system for the oral administration of antigens. They can protect antigens from degradation in the harsh environment of the gut. They can also improve the uptake of antigens by the immune system. Anti-GnRH vaccines work by blocking the binding of GnRH to its receptors on the cells of the hypothalamus and pituitary gland, preventing the release of LH and FSH and consequently suppress sex hormone production. Anti-GnRH injectable vaccines have been developed for a variety of production animals as an alternative to surgical castration. In this work we orally delivered polysaccharide microparticles containing a recombinant fusion protein as an antigen to develop Anti-GnRH response in male rat. We designed and expressed a recombinant 45 KDa peptide (GF45) consisting of a 4 tandem repeats from the GnRH sequence EHWSYGLRPG fused to an immunogenic carrier peptide which contains rickettsial Hsp70 and Hsp60 sequences. GF was encapsulated in alginate-chitosan particles by ionic reverse gelation. Particles containing 200 ug of recombinant peptide were orally administrated to a group of 4-week year old male Sprague-Dowley rats (n=10) in a prime-boost immunization scheme. Empty particles and injectable GF were used as controls. Serum testosterone and anti-GF45 antibodies were analysed by ELISA method along with the weight and volume of testicles. The GF45 orally treated group showed detectable titters of anti-GF45 antibodies and a 95% reduction of circulating testosterone in comparison to the control group. Testicles in treated group showed a reduction of 52% in weight and 50% in volume regarding to control. The encapsulation and oral delivery of the recombinant GF45 in alginate-chitosan microparticles generated autoimmune response capable of blocking the function of endogen GnRH. These particles are a suitable not only for generating non injectable or surgical control of GnRH but also to possibly deliver other recombinant antigens. Fil: Wilda, Maximiliano. Ministerio de Produccion y Trabajo. Secretaria de Gobierno de Agroindustria. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Centro de Virologia Animal. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Virologia Animal.; Argentina Fil: Grigera, Pablo Rafael. Ministerio de Produccion y Trabajo. Secretaria de Gobierno de Agroindustria. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Centro de Virologia Animal. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Virologia Animal.; Argentina 13th International Veterinary Immunology Symposium Mpumalanga Sudáfrica International Union of Immunological Societies |
| description |
Polysaccharide microparticles (PSMs) are a promising carrier system for the oral administration of antigens. They can protect antigens from degradation in the harsh environment of the gut. They can also improve the uptake of antigens by the immune system. Anti-GnRH vaccines work by blocking the binding of GnRH to its receptors on the cells of the hypothalamus and pituitary gland, preventing the release of LH and FSH and consequently suppress sex hormone production. Anti-GnRH injectable vaccines have been developed for a variety of production animals as an alternative to surgical castration. In this work we orally delivered polysaccharide microparticles containing a recombinant fusion protein as an antigen to develop Anti-GnRH response in male rat. We designed and expressed a recombinant 45 KDa peptide (GF45) consisting of a 4 tandem repeats from the GnRH sequence EHWSYGLRPG fused to an immunogenic carrier peptide which contains rickettsial Hsp70 and Hsp60 sequences. GF was encapsulated in alginate-chitosan particles by ionic reverse gelation. Particles containing 200 ug of recombinant peptide were orally administrated to a group of 4-week year old male Sprague-Dowley rats (n=10) in a prime-boost immunization scheme. Empty particles and injectable GF were used as controls. Serum testosterone and anti-GF45 antibodies were analysed by ELISA method along with the weight and volume of testicles. The GF45 orally treated group showed detectable titters of anti-GF45 antibodies and a 95% reduction of circulating testosterone in comparison to the control group. Testicles in treated group showed a reduction of 52% in weight and 50% in volume regarding to control. The encapsulation and oral delivery of the recombinant GF45 in alginate-chitosan microparticles generated autoimmune response capable of blocking the function of endogen GnRH. These particles are a suitable not only for generating non injectable or surgical control of GnRH but also to possibly deliver other recombinant antigens. |
| publishDate |
2023 |
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2023 |
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http://hdl.handle.net/11336/249500 Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male rats; 13th International Veterinary Immunology Symposium; Mpumalanga; Sudáfrica; 2023; 1-3 CONICET Digital CONICET |
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http://hdl.handle.net/11336/249500 |
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Polysaccharide microparticles as carriers of a recombinant antigen against GnRH in oral vaccination of male rats; 13th International Veterinary Immunology Symposium; Mpumalanga; Sudáfrica; 2023; 1-3 CONICET Digital CONICET |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/url/https://ivis2023.org/wp-content/uploads/2024/05/IVIS2023-Book-of-Abstracts_17-November-1.pdf |
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Internacional |
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International Union of Immunological Societies |
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International Union of Immunological Societies |
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