Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples

Autores
Ramirez, Eduardo Alejandro; Granero, Adrian Marcelo; Zon, María Alicia; Fernandez, Hector
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An amperometric biosensor based on Brassica napus hairy roots peroxidases (BHRP) to determine ochratoxin A (OTA) is proposed by the first time. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. The method employs a dialysis membrane covered, peroxidases entrapped and ferrocene-embedded carbon paste electrode (BHRP-Fc-CP) and is based on the decrease in the amount of H2O2 produced by the action of BHRP is proportional to the concentration of oxidised OTA in solution. BHRP-Fc-CP biosensors showed a very good stability during at least five days. The reproducibility and the repeatability were 7.7% and 5.4%, respectively, showing a good biosensor performance. Comparison between intra and inter assays showed a good accuracy. The calibration curve was linear in the OTA concentration range from 1 x 10-8 to 1.4 x 10-4 mol L-1. The lowest concentration experimentally measured for a signal to noise ratio of 3:1 was 5.7x10-9 mol L-1.
Fil: Ramirez, Eduardo Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Granero, Adrian Marcelo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Materia
OCHRATOXIN A
AMPEROMETRIC BIOSENSOR
BRASSICA NAPUS HAIRY ROOTS PEROXIDASES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195498
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195498
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samplesRamirez, Eduardo AlejandroGranero, Adrian MarceloZon, María AliciaFernandez, HectorOCHRATOXIN AAMPEROMETRIC BIOSENSORBRASSICA NAPUS HAIRY ROOTS PEROXIDASEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An amperometric biosensor based on Brassica napus hairy roots peroxidases (BHRP) to determine ochratoxin A (OTA) is proposed by the first time. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. The method employs a dialysis membrane covered, peroxidases entrapped and ferrocene-embedded carbon paste electrode (BHRP-Fc-CP) and is based on the decrease in the amount of H2O2 produced by the action of BHRP is proportional to the concentration of oxidised OTA in solution. BHRP-Fc-CP biosensors showed a very good stability during at least five days. The reproducibility and the repeatability were 7.7% and 5.4%, respectively, showing a good biosensor performance. Comparison between intra and inter assays showed a good accuracy. The calibration curve was linear in the OTA concentration range from 1 x 10-8 to 1.4 x 10-4 mol L-1. The lowest concentration experimentally measured for a signal to noise ratio of 3:1 was 5.7x10-9 mol L-1.Fil: Ramirez, Eduardo Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Granero, Adrian Marcelo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaOMICS GROUP2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195498Ramirez, Eduardo Alejandro; Granero, Adrian Marcelo; Zon, María Alicia; Fernandez, Hector; Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples; OMICS GROUP; Journal of Biosensors and Bioelectronics; s3; 11-2011; 1-62155-6210CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2155-6210.S3-001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:39:05Zoai:ri.conicet.gov.ar:11336/195498instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:39:05.383CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples
title Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples
spellingShingle Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples
Ramirez, Eduardo Alejandro
OCHRATOXIN A
AMPEROMETRIC BIOSENSOR
BRASSICA NAPUS HAIRY ROOTS PEROXIDASES
title_short Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples
title_full Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples
title_fullStr Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples
title_full_unstemmed Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples
title_sort Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples
dc.creator.none.fl_str_mv Ramirez, Eduardo Alejandro
Granero, Adrian Marcelo
Zon, María Alicia
Fernandez, Hector
author Ramirez, Eduardo Alejandro
author_facet Ramirez, Eduardo Alejandro
Granero, Adrian Marcelo
Zon, María Alicia
Fernandez, Hector
author_role author
author2 Granero, Adrian Marcelo
Zon, María Alicia
Fernandez, Hector
author2_role author
author
author
dc.subject.none.fl_str_mv OCHRATOXIN A
AMPEROMETRIC BIOSENSOR
BRASSICA NAPUS HAIRY ROOTS PEROXIDASES
topic OCHRATOXIN A
AMPEROMETRIC BIOSENSOR
BRASSICA NAPUS HAIRY ROOTS PEROXIDASES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An amperometric biosensor based on Brassica napus hairy roots peroxidases (BHRP) to determine ochratoxin A (OTA) is proposed by the first time. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. The method employs a dialysis membrane covered, peroxidases entrapped and ferrocene-embedded carbon paste electrode (BHRP-Fc-CP) and is based on the decrease in the amount of H2O2 produced by the action of BHRP is proportional to the concentration of oxidised OTA in solution. BHRP-Fc-CP biosensors showed a very good stability during at least five days. The reproducibility and the repeatability were 7.7% and 5.4%, respectively, showing a good biosensor performance. Comparison between intra and inter assays showed a good accuracy. The calibration curve was linear in the OTA concentration range from 1 x 10-8 to 1.4 x 10-4 mol L-1. The lowest concentration experimentally measured for a signal to noise ratio of 3:1 was 5.7x10-9 mol L-1.
Fil: Ramirez, Eduardo Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Granero, Adrian Marcelo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
description An amperometric biosensor based on Brassica napus hairy roots peroxidases (BHRP) to determine ochratoxin A (OTA) is proposed by the first time. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. The method employs a dialysis membrane covered, peroxidases entrapped and ferrocene-embedded carbon paste electrode (BHRP-Fc-CP) and is based on the decrease in the amount of H2O2 produced by the action of BHRP is proportional to the concentration of oxidised OTA in solution. BHRP-Fc-CP biosensors showed a very good stability during at least five days. The reproducibility and the repeatability were 7.7% and 5.4%, respectively, showing a good biosensor performance. Comparison between intra and inter assays showed a good accuracy. The calibration curve was linear in the OTA concentration range from 1 x 10-8 to 1.4 x 10-4 mol L-1. The lowest concentration experimentally measured for a signal to noise ratio of 3:1 was 5.7x10-9 mol L-1.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195498
Ramirez, Eduardo Alejandro; Granero, Adrian Marcelo; Zon, María Alicia; Fernandez, Hector; Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples; OMICS GROUP; Journal of Biosensors and Bioelectronics; s3; 11-2011; 1-6
2155-6210
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195498
identifier_str_mv Ramirez, Eduardo Alejandro; Granero, Adrian Marcelo; Zon, María Alicia; Fernandez, Hector; Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples; OMICS GROUP; Journal of Biosensors and Bioelectronics; s3; 11-2011; 1-6
2155-6210
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2155-6210.S3-001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv OMICS GROUP
publisher.none.fl_str_mv OMICS GROUP
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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