Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples
- Autores
- Ramirez, Eduardo Alejandro; Granero, Adrian Marcelo; Zon, María Alicia; Fernandez, Hector
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- An amperometric biosensor based on Brassica napus hairy roots peroxidases (BHRP) to determine ochratoxin A (OTA) is proposed by the first time. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. The method employs a dialysis membrane covered, peroxidases entrapped and ferrocene-embedded carbon paste electrode (BHRP-Fc-CP) and is based on the decrease in the amount of H2O2 produced by the action of BHRP is proportional to the concentration of oxidised OTA in solution. BHRP-Fc-CP biosensors showed a very good stability during at least five days. The reproducibility and the repeatability were 7.7% and 5.4%, respectively, showing a good biosensor performance. Comparison between intra and inter assays showed a good accuracy. The calibration curve was linear in the OTA concentration range from 1 x 10-8 to 1.4 x 10-4 mol L-1. The lowest concentration experimentally measured for a signal to noise ratio of 3:1 was 5.7x10-9 mol L-1.
Fil: Ramirez, Eduardo Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Granero, Adrian Marcelo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina - Materia
-
OCHRATOXIN A
AMPEROMETRIC BIOSENSOR
BRASSICA NAPUS HAIRY ROOTS PEROXIDASES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/195498
Ver los metadatos del registro completo
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Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samplesRamirez, Eduardo AlejandroGranero, Adrian MarceloZon, María AliciaFernandez, HectorOCHRATOXIN AAMPEROMETRIC BIOSENSORBRASSICA NAPUS HAIRY ROOTS PEROXIDASEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An amperometric biosensor based on Brassica napus hairy roots peroxidases (BHRP) to determine ochratoxin A (OTA) is proposed by the first time. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. The method employs a dialysis membrane covered, peroxidases entrapped and ferrocene-embedded carbon paste electrode (BHRP-Fc-CP) and is based on the decrease in the amount of H2O2 produced by the action of BHRP is proportional to the concentration of oxidised OTA in solution. BHRP-Fc-CP biosensors showed a very good stability during at least five days. The reproducibility and the repeatability were 7.7% and 5.4%, respectively, showing a good biosensor performance. Comparison between intra and inter assays showed a good accuracy. The calibration curve was linear in the OTA concentration range from 1 x 10-8 to 1.4 x 10-4 mol L-1. The lowest concentration experimentally measured for a signal to noise ratio of 3:1 was 5.7x10-9 mol L-1.Fil: Ramirez, Eduardo Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Granero, Adrian Marcelo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaOMICS GROUP2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195498Ramirez, Eduardo Alejandro; Granero, Adrian Marcelo; Zon, María Alicia; Fernandez, Hector; Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples; OMICS GROUP; Journal of Biosensors and Bioelectronics; s3; 11-2011; 1-62155-6210CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2155-6210.S3-001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:11Zoai:ri.conicet.gov.ar:11336/195498instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:11.722CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples |
title |
Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples |
spellingShingle |
Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples Ramirez, Eduardo Alejandro OCHRATOXIN A AMPEROMETRIC BIOSENSOR BRASSICA NAPUS HAIRY ROOTS PEROXIDASES |
title_short |
Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples |
title_full |
Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples |
title_fullStr |
Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples |
title_full_unstemmed |
Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples |
title_sort |
Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples |
dc.creator.none.fl_str_mv |
Ramirez, Eduardo Alejandro Granero, Adrian Marcelo Zon, María Alicia Fernandez, Hector |
author |
Ramirez, Eduardo Alejandro |
author_facet |
Ramirez, Eduardo Alejandro Granero, Adrian Marcelo Zon, María Alicia Fernandez, Hector |
author_role |
author |
author2 |
Granero, Adrian Marcelo Zon, María Alicia Fernandez, Hector |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
OCHRATOXIN A AMPEROMETRIC BIOSENSOR BRASSICA NAPUS HAIRY ROOTS PEROXIDASES |
topic |
OCHRATOXIN A AMPEROMETRIC BIOSENSOR BRASSICA NAPUS HAIRY ROOTS PEROXIDASES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
An amperometric biosensor based on Brassica napus hairy roots peroxidases (BHRP) to determine ochratoxin A (OTA) is proposed by the first time. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. The method employs a dialysis membrane covered, peroxidases entrapped and ferrocene-embedded carbon paste electrode (BHRP-Fc-CP) and is based on the decrease in the amount of H2O2 produced by the action of BHRP is proportional to the concentration of oxidised OTA in solution. BHRP-Fc-CP biosensors showed a very good stability during at least five days. The reproducibility and the repeatability were 7.7% and 5.4%, respectively, showing a good biosensor performance. Comparison between intra and inter assays showed a good accuracy. The calibration curve was linear in the OTA concentration range from 1 x 10-8 to 1.4 x 10-4 mol L-1. The lowest concentration experimentally measured for a signal to noise ratio of 3:1 was 5.7x10-9 mol L-1. Fil: Ramirez, Eduardo Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Granero, Adrian Marcelo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina |
description |
An amperometric biosensor based on Brassica napus hairy roots peroxidases (BHRP) to determine ochratoxin A (OTA) is proposed by the first time. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. The method employs a dialysis membrane covered, peroxidases entrapped and ferrocene-embedded carbon paste electrode (BHRP-Fc-CP) and is based on the decrease in the amount of H2O2 produced by the action of BHRP is proportional to the concentration of oxidised OTA in solution. BHRP-Fc-CP biosensors showed a very good stability during at least five days. The reproducibility and the repeatability were 7.7% and 5.4%, respectively, showing a good biosensor performance. Comparison between intra and inter assays showed a good accuracy. The calibration curve was linear in the OTA concentration range from 1 x 10-8 to 1.4 x 10-4 mol L-1. The lowest concentration experimentally measured for a signal to noise ratio of 3:1 was 5.7x10-9 mol L-1. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/195498 Ramirez, Eduardo Alejandro; Granero, Adrian Marcelo; Zon, María Alicia; Fernandez, Hector; Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples; OMICS GROUP; Journal of Biosensors and Bioelectronics; s3; 11-2011; 1-6 2155-6210 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/195498 |
identifier_str_mv |
Ramirez, Eduardo Alejandro; Granero, Adrian Marcelo; Zon, María Alicia; Fernandez, Hector; Development of an amperometric biosensor based on peroxidases from Brassicas napus for the determination of Ochratoxin a content in peanut samples; OMICS GROUP; Journal of Biosensors and Bioelectronics; s3; 11-2011; 1-6 2155-6210 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.4172/2155-6210.S3-001 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
OMICS GROUP |
publisher.none.fl_str_mv |
OMICS GROUP |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |