Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum

Autores
Mardirosian, Mariana Noelia; Lascano, Cecilia Ines; Ferrari, Ana; Bongiovanni, Guillermina Azucena; Venturino, Andres
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Arsenic (As), a natural element of ecological relevance, is found in natural water sources throughout Argentina in concentrations between 0.01mg/L and 15mg/L. The autochthonous toad Rhinella arenarum was selected to study the acute toxicity of As and the biochemical responses elicited by the exposure to As in water during its embryonic development. The median lethal concentration (LC50) value averaged 24.3mg/L As and remained constant along the embryonic development. However, As toxicity drastically decreased when embryos were exposed from heartbeat-stage on day 4 of development, suggesting the onset of detoxification mechanisms. Given the environmental concentrations of As in Argentina, there is a probability of exceeding lethal levels at 1% of sites. Arsenic at sublethal concentrations caused a significant decrease in the total antioxidant potential but generated an increase in endogenous glutathione (GSH) content and glutathione S-transferase (GST) activity. This protective response might prevent a deeper decline in the antioxidant system and further oxidative damage. Alternatively, it might be linked to As conjugation with GSH for its excretion. The authors conclude that toad embryos are more sensitive to As during early developmental stages and that relatively high concentrations of this toxic element are required to elicit mortality, but oxidative stress may be an adverse effect at sublethal concentrations.
Fil: Mardirosian, Mariana Noelia. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lascano, Cecilia Ines. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrari, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas; Argentina
Fil: Bongiovanni, Guillermina Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas; Argentina
Fil: Venturino, Andres. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
AMPHIBIANS
ANTIOXIDANT POTENTIAL
AQUATIC TOXICOLOGY
BIOMARKERS
GLUTATHIONE S-TRANSFERASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37115
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarumMardirosian, Mariana NoeliaLascano, Cecilia InesFerrari, AnaBongiovanni, Guillermina AzucenaVenturino, AndresAMPHIBIANSANTIOXIDANT POTENTIALAQUATIC TOXICOLOGYBIOMARKERSGLUTATHIONE S-TRANSFERASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Arsenic (As), a natural element of ecological relevance, is found in natural water sources throughout Argentina in concentrations between 0.01mg/L and 15mg/L. The autochthonous toad Rhinella arenarum was selected to study the acute toxicity of As and the biochemical responses elicited by the exposure to As in water during its embryonic development. The median lethal concentration (LC50) value averaged 24.3mg/L As and remained constant along the embryonic development. However, As toxicity drastically decreased when embryos were exposed from heartbeat-stage on day 4 of development, suggesting the onset of detoxification mechanisms. Given the environmental concentrations of As in Argentina, there is a probability of exceeding lethal levels at 1% of sites. Arsenic at sublethal concentrations caused a significant decrease in the total antioxidant potential but generated an increase in endogenous glutathione (GSH) content and glutathione S-transferase (GST) activity. This protective response might prevent a deeper decline in the antioxidant system and further oxidative damage. Alternatively, it might be linked to As conjugation with GSH for its excretion. The authors conclude that toad embryos are more sensitive to As during early developmental stages and that relatively high concentrations of this toxic element are required to elicit mortality, but oxidative stress may be an adverse effect at sublethal concentrations.Fil: Mardirosian, Mariana Noelia. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lascano, Cecilia Ines. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferrari, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas; ArgentinaFil: Bongiovanni, Guillermina Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas; ArgentinaFil: Venturino, Andres. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSociety of Environmental Toxicology and Chemistry2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37115Mardirosian, Mariana Noelia; Lascano, Cecilia Ines; Ferrari, Ana; Bongiovanni, Guillermina Azucena; Venturino, Andres; Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum; Society of Environmental Toxicology and Chemistry; Environmental Toxicology and Chemistry; 34; 5; 5-2015; 1009-10140730-7268CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/etc.2856info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/etc.2856/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:50Zoai:ri.conicet.gov.ar:11336/37115instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:50.266CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum
title Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum
spellingShingle Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum
Mardirosian, Mariana Noelia
AMPHIBIANS
ANTIOXIDANT POTENTIAL
AQUATIC TOXICOLOGY
BIOMARKERS
GLUTATHIONE S-TRANSFERASE
title_short Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum
title_full Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum
title_fullStr Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum
title_full_unstemmed Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum
title_sort Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum
dc.creator.none.fl_str_mv Mardirosian, Mariana Noelia
Lascano, Cecilia Ines
Ferrari, Ana
Bongiovanni, Guillermina Azucena
Venturino, Andres
author Mardirosian, Mariana Noelia
author_facet Mardirosian, Mariana Noelia
Lascano, Cecilia Ines
Ferrari, Ana
Bongiovanni, Guillermina Azucena
Venturino, Andres
author_role author
author2 Lascano, Cecilia Ines
Ferrari, Ana
Bongiovanni, Guillermina Azucena
Venturino, Andres
author2_role author
author
author
author
dc.subject.none.fl_str_mv AMPHIBIANS
ANTIOXIDANT POTENTIAL
AQUATIC TOXICOLOGY
BIOMARKERS
GLUTATHIONE S-TRANSFERASE
topic AMPHIBIANS
ANTIOXIDANT POTENTIAL
AQUATIC TOXICOLOGY
BIOMARKERS
GLUTATHIONE S-TRANSFERASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Arsenic (As), a natural element of ecological relevance, is found in natural water sources throughout Argentina in concentrations between 0.01mg/L and 15mg/L. The autochthonous toad Rhinella arenarum was selected to study the acute toxicity of As and the biochemical responses elicited by the exposure to As in water during its embryonic development. The median lethal concentration (LC50) value averaged 24.3mg/L As and remained constant along the embryonic development. However, As toxicity drastically decreased when embryos were exposed from heartbeat-stage on day 4 of development, suggesting the onset of detoxification mechanisms. Given the environmental concentrations of As in Argentina, there is a probability of exceeding lethal levels at 1% of sites. Arsenic at sublethal concentrations caused a significant decrease in the total antioxidant potential but generated an increase in endogenous glutathione (GSH) content and glutathione S-transferase (GST) activity. This protective response might prevent a deeper decline in the antioxidant system and further oxidative damage. Alternatively, it might be linked to As conjugation with GSH for its excretion. The authors conclude that toad embryos are more sensitive to As during early developmental stages and that relatively high concentrations of this toxic element are required to elicit mortality, but oxidative stress may be an adverse effect at sublethal concentrations.
Fil: Mardirosian, Mariana Noelia. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lascano, Cecilia Ines. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrari, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas; Argentina
Fil: Bongiovanni, Guillermina Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas; Argentina
Fil: Venturino, Andres. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Arsenic (As), a natural element of ecological relevance, is found in natural water sources throughout Argentina in concentrations between 0.01mg/L and 15mg/L. The autochthonous toad Rhinella arenarum was selected to study the acute toxicity of As and the biochemical responses elicited by the exposure to As in water during its embryonic development. The median lethal concentration (LC50) value averaged 24.3mg/L As and remained constant along the embryonic development. However, As toxicity drastically decreased when embryos were exposed from heartbeat-stage on day 4 of development, suggesting the onset of detoxification mechanisms. Given the environmental concentrations of As in Argentina, there is a probability of exceeding lethal levels at 1% of sites. Arsenic at sublethal concentrations caused a significant decrease in the total antioxidant potential but generated an increase in endogenous glutathione (GSH) content and glutathione S-transferase (GST) activity. This protective response might prevent a deeper decline in the antioxidant system and further oxidative damage. Alternatively, it might be linked to As conjugation with GSH for its excretion. The authors conclude that toad embryos are more sensitive to As during early developmental stages and that relatively high concentrations of this toxic element are required to elicit mortality, but oxidative stress may be an adverse effect at sublethal concentrations.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37115
Mardirosian, Mariana Noelia; Lascano, Cecilia Ines; Ferrari, Ana; Bongiovanni, Guillermina Azucena; Venturino, Andres; Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum; Society of Environmental Toxicology and Chemistry; Environmental Toxicology and Chemistry; 34; 5; 5-2015; 1009-1014
0730-7268
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37115
identifier_str_mv Mardirosian, Mariana Noelia; Lascano, Cecilia Ines; Ferrari, Ana; Bongiovanni, Guillermina Azucena; Venturino, Andres; Acute toxicity of arsenic and oxidative stress responses in the embryonic development of the common South American toad Rhinella arenarum; Society of Environmental Toxicology and Chemistry; Environmental Toxicology and Chemistry; 34; 5; 5-2015; 1009-1014
0730-7268
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/etc.2856
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/etc.2856/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Society of Environmental Toxicology and Chemistry
publisher.none.fl_str_mv Society of Environmental Toxicology and Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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