Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin
- Autores
- Carriquiriborde, Pedro; Marino, Damian Jose Gabriel; Giachero; Castro, Eduardo Alberto; Ronco, Alicia Estela
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The metabolic profile of Odontesthes bonariensis and its global response to the insecticide cypermethrin were studied using HPLC-MS-based metabolomics. Three experiments using either juveniles or adults of O. bonariensis were performed by exposing fish (6, 24, or 96h) to sublethal concentrations of cypermethrin (5 or 10μg/L). Metabolic profiling was performed on either whole bile or aqueous and organic extracts. Chromatography was performed using a C18 column and an ACN/H2O mobile phase. Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) interfaces were used in positive and negative modes. Full scan MS data were processed using the XCMS software, log-transformed, and analyzed using either regression analysis or principal component analysis (PCA). The highest amount of information (1163 peaks) was yielded by analyzing the whole bile with the ESI(-) interface. Complementary information, useful for metabolite confirmation, was obtained from the aqueous and organic extracts and using the ESI(+) and APCI interfaces. The bile metabolic profile of O. bonariensis was characterized by some abundant metabolite ions corresponding with taurine conjugated bile acids, which were useful as reference peaks. A characteristic global metabolic response to cypermethrin was identified in the bile of O. bonariensis. A ten-fold or higher variation in abundance was observed in the whole bile of exposed fish for a small group of peaks (32), and these peaks corresponded to an even smaller number of metabolites (nineteen). Both regression analysis and PCA were useful in identifying those peaks, better explaining differences between exposed and control groups, but slight differences were suggested by each of those methods. Using unsupervised PCA scores, we were able to distinguish organisms from each treatment on the basis of the metabolic changes induced by the cypermethrin, this variability being explained mainly by only one principal component (PC3, 17.7 percent total variance). Two cypermethrin metabolites were identified as major contributors within the augmented peaks: the known glucuronide of 4'-hydroxy-cypermethrin and the sulfate of 4'-hydroxycypermethrin, not previously reported in fish bile. The HPLC-MS-based metabolomic approach demonstrated to be a powerful ecotoxicological tool for identifying biological responses to pollutants, discovering new metabolic pathways and proposing specific biomarkers using non model organisms.
Fil: Carriquiriborde, Pedro. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marino, Damian Jose Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Giachero. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina
Fil: Castro, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Ronco, Alicia Estela. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Aquatic Toxicology
Biomarkers
Ecotoxicology
Fish Toxicology
Metabolomics
Pesticides - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/76682
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Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrinCarriquiriborde, PedroMarino, Damian Jose GabrielGiacheroCastro, Eduardo AlbertoRonco, Alicia EstelaAquatic ToxicologyBiomarkersEcotoxicologyFish ToxicologyMetabolomicsPesticideshttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1The metabolic profile of Odontesthes bonariensis and its global response to the insecticide cypermethrin were studied using HPLC-MS-based metabolomics. Three experiments using either juveniles or adults of O. bonariensis were performed by exposing fish (6, 24, or 96h) to sublethal concentrations of cypermethrin (5 or 10μg/L). Metabolic profiling was performed on either whole bile or aqueous and organic extracts. Chromatography was performed using a C18 column and an ACN/H2O mobile phase. Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) interfaces were used in positive and negative modes. Full scan MS data were processed using the XCMS software, log-transformed, and analyzed using either regression analysis or principal component analysis (PCA). The highest amount of information (1163 peaks) was yielded by analyzing the whole bile with the ESI(-) interface. Complementary information, useful for metabolite confirmation, was obtained from the aqueous and organic extracts and using the ESI(+) and APCI interfaces. The bile metabolic profile of O. bonariensis was characterized by some abundant metabolite ions corresponding with taurine conjugated bile acids, which were useful as reference peaks. A characteristic global metabolic response to cypermethrin was identified in the bile of O. bonariensis. A ten-fold or higher variation in abundance was observed in the whole bile of exposed fish for a small group of peaks (32), and these peaks corresponded to an even smaller number of metabolites (nineteen). Both regression analysis and PCA were useful in identifying those peaks, better explaining differences between exposed and control groups, but slight differences were suggested by each of those methods. Using unsupervised PCA scores, we were able to distinguish organisms from each treatment on the basis of the metabolic changes induced by the cypermethrin, this variability being explained mainly by only one principal component (PC3, 17.7 percent total variance). Two cypermethrin metabolites were identified as major contributors within the augmented peaks: the known glucuronide of 4'-hydroxy-cypermethrin and the sulfate of 4'-hydroxycypermethrin, not previously reported in fish bile. The HPLC-MS-based metabolomic approach demonstrated to be a powerful ecotoxicological tool for identifying biological responses to pollutants, discovering new metabolic pathways and proposing specific biomarkers using non model organisms.Fil: Carriquiriborde, Pedro. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marino, Damian Jose Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Giachero. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; ArgentinaFil: Castro, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Ronco, Alicia Estela. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAcademic Press Inc Elsevier Science2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76682Carriquiriborde, Pedro; Marino, Damian Jose Gabriel; Giachero; Castro, Eduardo Alberto; Ronco, Alicia Estela; Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 76; 1; 2-2012; 46-540147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2011.07.039info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651311002570info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:14Zoai:ri.conicet.gov.ar:11336/76682instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:14.869CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin |
title |
Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin |
spellingShingle |
Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin Carriquiriborde, Pedro Aquatic Toxicology Biomarkers Ecotoxicology Fish Toxicology Metabolomics Pesticides |
title_short |
Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin |
title_full |
Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin |
title_fullStr |
Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin |
title_full_unstemmed |
Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin |
title_sort |
Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin |
dc.creator.none.fl_str_mv |
Carriquiriborde, Pedro Marino, Damian Jose Gabriel Giachero Castro, Eduardo Alberto Ronco, Alicia Estela |
author |
Carriquiriborde, Pedro |
author_facet |
Carriquiriborde, Pedro Marino, Damian Jose Gabriel Giachero Castro, Eduardo Alberto Ronco, Alicia Estela |
author_role |
author |
author2 |
Marino, Damian Jose Gabriel Giachero Castro, Eduardo Alberto Ronco, Alicia Estela |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Aquatic Toxicology Biomarkers Ecotoxicology Fish Toxicology Metabolomics Pesticides |
topic |
Aquatic Toxicology Biomarkers Ecotoxicology Fish Toxicology Metabolomics Pesticides |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.5 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The metabolic profile of Odontesthes bonariensis and its global response to the insecticide cypermethrin were studied using HPLC-MS-based metabolomics. Three experiments using either juveniles or adults of O. bonariensis were performed by exposing fish (6, 24, or 96h) to sublethal concentrations of cypermethrin (5 or 10μg/L). Metabolic profiling was performed on either whole bile or aqueous and organic extracts. Chromatography was performed using a C18 column and an ACN/H2O mobile phase. Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) interfaces were used in positive and negative modes. Full scan MS data were processed using the XCMS software, log-transformed, and analyzed using either regression analysis or principal component analysis (PCA). The highest amount of information (1163 peaks) was yielded by analyzing the whole bile with the ESI(-) interface. Complementary information, useful for metabolite confirmation, was obtained from the aqueous and organic extracts and using the ESI(+) and APCI interfaces. The bile metabolic profile of O. bonariensis was characterized by some abundant metabolite ions corresponding with taurine conjugated bile acids, which were useful as reference peaks. A characteristic global metabolic response to cypermethrin was identified in the bile of O. bonariensis. A ten-fold or higher variation in abundance was observed in the whole bile of exposed fish for a small group of peaks (32), and these peaks corresponded to an even smaller number of metabolites (nineteen). Both regression analysis and PCA were useful in identifying those peaks, better explaining differences between exposed and control groups, but slight differences were suggested by each of those methods. Using unsupervised PCA scores, we were able to distinguish organisms from each treatment on the basis of the metabolic changes induced by the cypermethrin, this variability being explained mainly by only one principal component (PC3, 17.7 percent total variance). Two cypermethrin metabolites were identified as major contributors within the augmented peaks: the known glucuronide of 4'-hydroxy-cypermethrin and the sulfate of 4'-hydroxycypermethrin, not previously reported in fish bile. The HPLC-MS-based metabolomic approach demonstrated to be a powerful ecotoxicological tool for identifying biological responses to pollutants, discovering new metabolic pathways and proposing specific biomarkers using non model organisms. Fil: Carriquiriborde, Pedro. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marino, Damian Jose Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Giachero. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina Fil: Castro, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Ronco, Alicia Estela. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
The metabolic profile of Odontesthes bonariensis and its global response to the insecticide cypermethrin were studied using HPLC-MS-based metabolomics. Three experiments using either juveniles or adults of O. bonariensis were performed by exposing fish (6, 24, or 96h) to sublethal concentrations of cypermethrin (5 or 10μg/L). Metabolic profiling was performed on either whole bile or aqueous and organic extracts. Chromatography was performed using a C18 column and an ACN/H2O mobile phase. Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) interfaces were used in positive and negative modes. Full scan MS data were processed using the XCMS software, log-transformed, and analyzed using either regression analysis or principal component analysis (PCA). The highest amount of information (1163 peaks) was yielded by analyzing the whole bile with the ESI(-) interface. Complementary information, useful for metabolite confirmation, was obtained from the aqueous and organic extracts and using the ESI(+) and APCI interfaces. The bile metabolic profile of O. bonariensis was characterized by some abundant metabolite ions corresponding with taurine conjugated bile acids, which were useful as reference peaks. A characteristic global metabolic response to cypermethrin was identified in the bile of O. bonariensis. A ten-fold or higher variation in abundance was observed in the whole bile of exposed fish for a small group of peaks (32), and these peaks corresponded to an even smaller number of metabolites (nineteen). Both regression analysis and PCA were useful in identifying those peaks, better explaining differences between exposed and control groups, but slight differences were suggested by each of those methods. Using unsupervised PCA scores, we were able to distinguish organisms from each treatment on the basis of the metabolic changes induced by the cypermethrin, this variability being explained mainly by only one principal component (PC3, 17.7 percent total variance). Two cypermethrin metabolites were identified as major contributors within the augmented peaks: the known glucuronide of 4'-hydroxy-cypermethrin and the sulfate of 4'-hydroxycypermethrin, not previously reported in fish bile. The HPLC-MS-based metabolomic approach demonstrated to be a powerful ecotoxicological tool for identifying biological responses to pollutants, discovering new metabolic pathways and proposing specific biomarkers using non model organisms. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/76682 Carriquiriborde, Pedro; Marino, Damian Jose Gabriel; Giachero; Castro, Eduardo Alberto; Ronco, Alicia Estela; Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 76; 1; 2-2012; 46-54 0147-6513 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/76682 |
identifier_str_mv |
Carriquiriborde, Pedro; Marino, Damian Jose Gabriel; Giachero; Castro, Eduardo Alberto; Ronco, Alicia Estela; Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 76; 1; 2-2012; 46-54 0147-6513 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2011.07.039 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651311002570 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268779798593536 |
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13.13397 |