Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin

Autores
Carriquiriborde, Pedro; Marino, Damian Jose Gabriel; Giachero; Castro, Eduardo Alberto; Ronco, Alicia Estela
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The metabolic profile of Odontesthes bonariensis and its global response to the insecticide cypermethrin were studied using HPLC-MS-based metabolomics. Three experiments using either juveniles or adults of O. bonariensis were performed by exposing fish (6, 24, or 96h) to sublethal concentrations of cypermethrin (5 or 10μg/L). Metabolic profiling was performed on either whole bile or aqueous and organic extracts. Chromatography was performed using a C18 column and an ACN/H2O mobile phase. Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) interfaces were used in positive and negative modes. Full scan MS data were processed using the XCMS software, log-transformed, and analyzed using either regression analysis or principal component analysis (PCA). The highest amount of information (1163 peaks) was yielded by analyzing the whole bile with the ESI(-) interface. Complementary information, useful for metabolite confirmation, was obtained from the aqueous and organic extracts and using the ESI(+) and APCI interfaces. The bile metabolic profile of O. bonariensis was characterized by some abundant metabolite ions corresponding with taurine conjugated bile acids, which were useful as reference peaks. A characteristic global metabolic response to cypermethrin was identified in the bile of O. bonariensis. A ten-fold or higher variation in abundance was observed in the whole bile of exposed fish for a small group of peaks (32), and these peaks corresponded to an even smaller number of metabolites (nineteen). Both regression analysis and PCA were useful in identifying those peaks, better explaining differences between exposed and control groups, but slight differences were suggested by each of those methods. Using unsupervised PCA scores, we were able to distinguish organisms from each treatment on the basis of the metabolic changes induced by the cypermethrin, this variability being explained mainly by only one principal component (PC3, 17.7 percent total variance). Two cypermethrin metabolites were identified as major contributors within the augmented peaks: the known glucuronide of 4'-hydroxy-cypermethrin and the sulfate of 4'-hydroxycypermethrin, not previously reported in fish bile. The HPLC-MS-based metabolomic approach demonstrated to be a powerful ecotoxicological tool for identifying biological responses to pollutants, discovering new metabolic pathways and proposing specific biomarkers using non model organisms.
Fil: Carriquiriborde, Pedro. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marino, Damian Jose Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Giachero. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina
Fil: Castro, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Ronco, Alicia Estela. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Aquatic Toxicology
Biomarkers
Ecotoxicology
Fish Toxicology
Metabolomics
Pesticides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/76682

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network_name_str CONICET Digital (CONICET)
spelling Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrinCarriquiriborde, PedroMarino, Damian Jose GabrielGiacheroCastro, Eduardo AlbertoRonco, Alicia EstelaAquatic ToxicologyBiomarkersEcotoxicologyFish ToxicologyMetabolomicsPesticideshttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1The metabolic profile of Odontesthes bonariensis and its global response to the insecticide cypermethrin were studied using HPLC-MS-based metabolomics. Three experiments using either juveniles or adults of O. bonariensis were performed by exposing fish (6, 24, or 96h) to sublethal concentrations of cypermethrin (5 or 10μg/L). Metabolic profiling was performed on either whole bile or aqueous and organic extracts. Chromatography was performed using a C18 column and an ACN/H2O mobile phase. Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) interfaces were used in positive and negative modes. Full scan MS data were processed using the XCMS software, log-transformed, and analyzed using either regression analysis or principal component analysis (PCA). The highest amount of information (1163 peaks) was yielded by analyzing the whole bile with the ESI(-) interface. Complementary information, useful for metabolite confirmation, was obtained from the aqueous and organic extracts and using the ESI(+) and APCI interfaces. The bile metabolic profile of O. bonariensis was characterized by some abundant metabolite ions corresponding with taurine conjugated bile acids, which were useful as reference peaks. A characteristic global metabolic response to cypermethrin was identified in the bile of O. bonariensis. A ten-fold or higher variation in abundance was observed in the whole bile of exposed fish for a small group of peaks (32), and these peaks corresponded to an even smaller number of metabolites (nineteen). Both regression analysis and PCA were useful in identifying those peaks, better explaining differences between exposed and control groups, but slight differences were suggested by each of those methods. Using unsupervised PCA scores, we were able to distinguish organisms from each treatment on the basis of the metabolic changes induced by the cypermethrin, this variability being explained mainly by only one principal component (PC3, 17.7 percent total variance). Two cypermethrin metabolites were identified as major contributors within the augmented peaks: the known glucuronide of 4'-hydroxy-cypermethrin and the sulfate of 4'-hydroxycypermethrin, not previously reported in fish bile. The HPLC-MS-based metabolomic approach demonstrated to be a powerful ecotoxicological tool for identifying biological responses to pollutants, discovering new metabolic pathways and proposing specific biomarkers using non model organisms.Fil: Carriquiriborde, Pedro. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marino, Damian Jose Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Giachero. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; ArgentinaFil: Castro, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Ronco, Alicia Estela. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAcademic Press Inc Elsevier Science2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76682Carriquiriborde, Pedro; Marino, Damian Jose Gabriel; Giachero; Castro, Eduardo Alberto; Ronco, Alicia Estela; Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 76; 1; 2-2012; 46-540147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2011.07.039info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651311002570info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:14Zoai:ri.conicet.gov.ar:11336/76682instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:14.869CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin
title Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin
spellingShingle Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin
Carriquiriborde, Pedro
Aquatic Toxicology
Biomarkers
Ecotoxicology
Fish Toxicology
Metabolomics
Pesticides
title_short Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin
title_full Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin
title_fullStr Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin
title_full_unstemmed Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin
title_sort Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin
dc.creator.none.fl_str_mv Carriquiriborde, Pedro
Marino, Damian Jose Gabriel
Giachero
Castro, Eduardo Alberto
Ronco, Alicia Estela
author Carriquiriborde, Pedro
author_facet Carriquiriborde, Pedro
Marino, Damian Jose Gabriel
Giachero
Castro, Eduardo Alberto
Ronco, Alicia Estela
author_role author
author2 Marino, Damian Jose Gabriel
Giachero
Castro, Eduardo Alberto
Ronco, Alicia Estela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Aquatic Toxicology
Biomarkers
Ecotoxicology
Fish Toxicology
Metabolomics
Pesticides
topic Aquatic Toxicology
Biomarkers
Ecotoxicology
Fish Toxicology
Metabolomics
Pesticides
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The metabolic profile of Odontesthes bonariensis and its global response to the insecticide cypermethrin were studied using HPLC-MS-based metabolomics. Three experiments using either juveniles or adults of O. bonariensis were performed by exposing fish (6, 24, or 96h) to sublethal concentrations of cypermethrin (5 or 10μg/L). Metabolic profiling was performed on either whole bile or aqueous and organic extracts. Chromatography was performed using a C18 column and an ACN/H2O mobile phase. Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) interfaces were used in positive and negative modes. Full scan MS data were processed using the XCMS software, log-transformed, and analyzed using either regression analysis or principal component analysis (PCA). The highest amount of information (1163 peaks) was yielded by analyzing the whole bile with the ESI(-) interface. Complementary information, useful for metabolite confirmation, was obtained from the aqueous and organic extracts and using the ESI(+) and APCI interfaces. The bile metabolic profile of O. bonariensis was characterized by some abundant metabolite ions corresponding with taurine conjugated bile acids, which were useful as reference peaks. A characteristic global metabolic response to cypermethrin was identified in the bile of O. bonariensis. A ten-fold or higher variation in abundance was observed in the whole bile of exposed fish for a small group of peaks (32), and these peaks corresponded to an even smaller number of metabolites (nineteen). Both regression analysis and PCA were useful in identifying those peaks, better explaining differences between exposed and control groups, but slight differences were suggested by each of those methods. Using unsupervised PCA scores, we were able to distinguish organisms from each treatment on the basis of the metabolic changes induced by the cypermethrin, this variability being explained mainly by only one principal component (PC3, 17.7 percent total variance). Two cypermethrin metabolites were identified as major contributors within the augmented peaks: the known glucuronide of 4'-hydroxy-cypermethrin and the sulfate of 4'-hydroxycypermethrin, not previously reported in fish bile. The HPLC-MS-based metabolomic approach demonstrated to be a powerful ecotoxicological tool for identifying biological responses to pollutants, discovering new metabolic pathways and proposing specific biomarkers using non model organisms.
Fil: Carriquiriborde, Pedro. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marino, Damian Jose Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Giachero. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina
Fil: Castro, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Ronco, Alicia Estela. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The metabolic profile of Odontesthes bonariensis and its global response to the insecticide cypermethrin were studied using HPLC-MS-based metabolomics. Three experiments using either juveniles or adults of O. bonariensis were performed by exposing fish (6, 24, or 96h) to sublethal concentrations of cypermethrin (5 or 10μg/L). Metabolic profiling was performed on either whole bile or aqueous and organic extracts. Chromatography was performed using a C18 column and an ACN/H2O mobile phase. Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) interfaces were used in positive and negative modes. Full scan MS data were processed using the XCMS software, log-transformed, and analyzed using either regression analysis or principal component analysis (PCA). The highest amount of information (1163 peaks) was yielded by analyzing the whole bile with the ESI(-) interface. Complementary information, useful for metabolite confirmation, was obtained from the aqueous and organic extracts and using the ESI(+) and APCI interfaces. The bile metabolic profile of O. bonariensis was characterized by some abundant metabolite ions corresponding with taurine conjugated bile acids, which were useful as reference peaks. A characteristic global metabolic response to cypermethrin was identified in the bile of O. bonariensis. A ten-fold or higher variation in abundance was observed in the whole bile of exposed fish for a small group of peaks (32), and these peaks corresponded to an even smaller number of metabolites (nineteen). Both regression analysis and PCA were useful in identifying those peaks, better explaining differences between exposed and control groups, but slight differences were suggested by each of those methods. Using unsupervised PCA scores, we were able to distinguish organisms from each treatment on the basis of the metabolic changes induced by the cypermethrin, this variability being explained mainly by only one principal component (PC3, 17.7 percent total variance). Two cypermethrin metabolites were identified as major contributors within the augmented peaks: the known glucuronide of 4'-hydroxy-cypermethrin and the sulfate of 4'-hydroxycypermethrin, not previously reported in fish bile. The HPLC-MS-based metabolomic approach demonstrated to be a powerful ecotoxicological tool for identifying biological responses to pollutants, discovering new metabolic pathways and proposing specific biomarkers using non model organisms.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/76682
Carriquiriborde, Pedro; Marino, Damian Jose Gabriel; Giachero; Castro, Eduardo Alberto; Ronco, Alicia Estela; Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 76; 1; 2-2012; 46-54
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/76682
identifier_str_mv Carriquiriborde, Pedro; Marino, Damian Jose Gabriel; Giachero; Castro, Eduardo Alberto; Ronco, Alicia Estela; Global metabolic response in the bile of pejerrey (Odontesthes bonariensis, Pisces) sublethally exposed to the pyrethroid cypermethrin; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 76; 1; 2-2012; 46-54
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2011.07.039
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651311002570
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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