First report of Penicillium allii as a field pathogen of garlic (Allium sativum)

Autores
Valdez, Jorge Gustavo; Makuch, M. A.; Ordovini, A. F.; Masuelli, Ricardo Williams; Overy, D. P.; Piccolo, Ricardo
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Argentina is the world´s second largest exporter of garlic (Allium sativum) and blue mold rot is annually responsible for significant crop losses. Diseased plants appear stunted and chlorotic with withered leaves and a reduction in bulb size as well as bulbs often being covered with blue/green conidial masses. To identify the pathogen, symptomatic plants (Figure 1) were sampled in the field at La Consulta station (33°45´ S, 69°02´ W). Visible fungi were cultured and identified as P. allii. Representative strains IBT# 26466, 26467, 26511 and 26512 were accessioned into the IBT collection, BioCentrum-DTU. Identifications were confirmed by comparing RP-HPLC secondary metabolite profiles with those of standard and ex Type P. allii strains (IBT 3772, 4112 and 21503). To confirm pathogenicity sterilized garlic cloves inoculated with the P. allii strains (1.5 mm deep, 5 µL of a suspension with 5x106 con.mL-1) were planted in a randomized design (4 replications plus an un-inoculated control) in a field not previously cultivated with garlic. The survival rate of inoculated plants was 68% with an overall yield of 30% marketable bulbs. P. allii was re-isolated from symptomatic field plants. Micro-morphology and yellow exudate production (Onion, 1981), were previously used to identify P. viridicatum as a pathogen of garlic in Argentina (Gatica and Oriolani, 1984); however at that time P. allii was not yet characterized. P. allii is micro-morphologically similar to P. viridicatum and also produces yellow exudate (Vincent & Pitt, 1989). To compare pathogenic ability, P. viridicatum (IBT 16939 and IBT 15053) and P. allii strains were inoculated into sterilized garlic cloves and incubated for 12 d. P. viridicatum strains were not pathogenic meanwhile P. allii strains sporulated on the garlic cloves. P. allii has been reported as an aggressive pathogen of garlic in pathogenicity trials conducted in moisture chambers (Overy et al., 2005).
Fil: Valdez, Jorge Gustavo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Makuch, M. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Ordovini, A. F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Overy, D. P.. Technical University of Denmark; Dinamarca. University of Wales; Reino Unido
Fil: Piccolo, Ricardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Materia
Garlic
Plant Pathology
Penicillium
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147639
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/147639
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First report of Penicillium allii as a field pathogen of garlic (Allium sativum)Valdez, Jorge GustavoMakuch, M. A.Ordovini, A. F.Masuelli, Ricardo WilliamsOvery, D. P.Piccolo, RicardoGarlicPlant PathologyPenicilliumhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Argentina is the world´s second largest exporter of garlic (Allium sativum) and blue mold rot is annually responsible for significant crop losses. Diseased plants appear stunted and chlorotic with withered leaves and a reduction in bulb size as well as bulbs often being covered with blue/green conidial masses. To identify the pathogen, symptomatic plants (Figure 1) were sampled in the field at La Consulta station (33°45´ S, 69°02´ W). Visible fungi were cultured and identified as P. allii. Representative strains IBT# 26466, 26467, 26511 and 26512 were accessioned into the IBT collection, BioCentrum-DTU. Identifications were confirmed by comparing RP-HPLC secondary metabolite profiles with those of standard and ex Type P. allii strains (IBT 3772, 4112 and 21503). To confirm pathogenicity sterilized garlic cloves inoculated with the P. allii strains (1.5 mm deep, 5 µL of a suspension with 5x106 con.mL-1) were planted in a randomized design (4 replications plus an un-inoculated control) in a field not previously cultivated with garlic. The survival rate of inoculated plants was 68% with an overall yield of 30% marketable bulbs. P. allii was re-isolated from symptomatic field plants. Micro-morphology and yellow exudate production (Onion, 1981), were previously used to identify P. viridicatum as a pathogen of garlic in Argentina (Gatica and Oriolani, 1984); however at that time P. allii was not yet characterized. P. allii is micro-morphologically similar to P. viridicatum and also produces yellow exudate (Vincent & Pitt, 1989). To compare pathogenic ability, P. viridicatum (IBT 16939 and IBT 15053) and P. allii strains were inoculated into sterilized garlic cloves and incubated for 12 d. P. viridicatum strains were not pathogenic meanwhile P. allii strains sporulated on the garlic cloves. P. allii has been reported as an aggressive pathogen of garlic in pathogenicity trials conducted in moisture chambers (Overy et al., 2005).Fil: Valdez, Jorge Gustavo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Makuch, M. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Ordovini, A. F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Overy, D. P.. Technical University of Denmark; Dinamarca. University of Wales; Reino UnidoFil: Piccolo, Ricardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; ArgentinaWiley Blackwell Publishing, Inc2006-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147639Valdez, Jorge Gustavo; Makuch, M. A.; Ordovini, A. F.; Masuelli, Ricardo Williams; Overy, D. P.; et al.; First report of Penicillium allii as a field pathogen of garlic (Allium sativum); Wiley Blackwell Publishing, Inc; Plant Pathology; 55; 4; 8-2006; 583-5830032-0862CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2006.01411.x/fullinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-3059.2006.01411.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:55Zoai:ri.conicet.gov.ar:11336/147639instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:56.056CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First report of Penicillium allii as a field pathogen of garlic (Allium sativum)
title First report of Penicillium allii as a field pathogen of garlic (Allium sativum)
spellingShingle First report of Penicillium allii as a field pathogen of garlic (Allium sativum)
Valdez, Jorge Gustavo
Garlic
Plant Pathology
Penicillium
title_short First report of Penicillium allii as a field pathogen of garlic (Allium sativum)
title_full First report of Penicillium allii as a field pathogen of garlic (Allium sativum)
title_fullStr First report of Penicillium allii as a field pathogen of garlic (Allium sativum)
title_full_unstemmed First report of Penicillium allii as a field pathogen of garlic (Allium sativum)
title_sort First report of Penicillium allii as a field pathogen of garlic (Allium sativum)
dc.creator.none.fl_str_mv Valdez, Jorge Gustavo
Makuch, M. A.
Ordovini, A. F.
Masuelli, Ricardo Williams
Overy, D. P.
Piccolo, Ricardo
author Valdez, Jorge Gustavo
author_facet Valdez, Jorge Gustavo
Makuch, M. A.
Ordovini, A. F.
Masuelli, Ricardo Williams
Overy, D. P.
Piccolo, Ricardo
author_role author
author2 Makuch, M. A.
Ordovini, A. F.
Masuelli, Ricardo Williams
Overy, D. P.
Piccolo, Ricardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Garlic
Plant Pathology
Penicillium
topic Garlic
Plant Pathology
Penicillium
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Argentina is the world´s second largest exporter of garlic (Allium sativum) and blue mold rot is annually responsible for significant crop losses. Diseased plants appear stunted and chlorotic with withered leaves and a reduction in bulb size as well as bulbs often being covered with blue/green conidial masses. To identify the pathogen, symptomatic plants (Figure 1) were sampled in the field at La Consulta station (33°45´ S, 69°02´ W). Visible fungi were cultured and identified as P. allii. Representative strains IBT# 26466, 26467, 26511 and 26512 were accessioned into the IBT collection, BioCentrum-DTU. Identifications were confirmed by comparing RP-HPLC secondary metabolite profiles with those of standard and ex Type P. allii strains (IBT 3772, 4112 and 21503). To confirm pathogenicity sterilized garlic cloves inoculated with the P. allii strains (1.5 mm deep, 5 µL of a suspension with 5x106 con.mL-1) were planted in a randomized design (4 replications plus an un-inoculated control) in a field not previously cultivated with garlic. The survival rate of inoculated plants was 68% with an overall yield of 30% marketable bulbs. P. allii was re-isolated from symptomatic field plants. Micro-morphology and yellow exudate production (Onion, 1981), were previously used to identify P. viridicatum as a pathogen of garlic in Argentina (Gatica and Oriolani, 1984); however at that time P. allii was not yet characterized. P. allii is micro-morphologically similar to P. viridicatum and also produces yellow exudate (Vincent & Pitt, 1989). To compare pathogenic ability, P. viridicatum (IBT 16939 and IBT 15053) and P. allii strains were inoculated into sterilized garlic cloves and incubated for 12 d. P. viridicatum strains were not pathogenic meanwhile P. allii strains sporulated on the garlic cloves. P. allii has been reported as an aggressive pathogen of garlic in pathogenicity trials conducted in moisture chambers (Overy et al., 2005).
Fil: Valdez, Jorge Gustavo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Makuch, M. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Ordovini, A. F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Overy, D. P.. Technical University of Denmark; Dinamarca. University of Wales; Reino Unido
Fil: Piccolo, Ricardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
description Argentina is the world´s second largest exporter of garlic (Allium sativum) and blue mold rot is annually responsible for significant crop losses. Diseased plants appear stunted and chlorotic with withered leaves and a reduction in bulb size as well as bulbs often being covered with blue/green conidial masses. To identify the pathogen, symptomatic plants (Figure 1) were sampled in the field at La Consulta station (33°45´ S, 69°02´ W). Visible fungi were cultured and identified as P. allii. Representative strains IBT# 26466, 26467, 26511 and 26512 were accessioned into the IBT collection, BioCentrum-DTU. Identifications were confirmed by comparing RP-HPLC secondary metabolite profiles with those of standard and ex Type P. allii strains (IBT 3772, 4112 and 21503). To confirm pathogenicity sterilized garlic cloves inoculated with the P. allii strains (1.5 mm deep, 5 µL of a suspension with 5x106 con.mL-1) were planted in a randomized design (4 replications plus an un-inoculated control) in a field not previously cultivated with garlic. The survival rate of inoculated plants was 68% with an overall yield of 30% marketable bulbs. P. allii was re-isolated from symptomatic field plants. Micro-morphology and yellow exudate production (Onion, 1981), were previously used to identify P. viridicatum as a pathogen of garlic in Argentina (Gatica and Oriolani, 1984); however at that time P. allii was not yet characterized. P. allii is micro-morphologically similar to P. viridicatum and also produces yellow exudate (Vincent & Pitt, 1989). To compare pathogenic ability, P. viridicatum (IBT 16939 and IBT 15053) and P. allii strains were inoculated into sterilized garlic cloves and incubated for 12 d. P. viridicatum strains were not pathogenic meanwhile P. allii strains sporulated on the garlic cloves. P. allii has been reported as an aggressive pathogen of garlic in pathogenicity trials conducted in moisture chambers (Overy et al., 2005).
publishDate 2006
dc.date.none.fl_str_mv 2006-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147639
Valdez, Jorge Gustavo; Makuch, M. A.; Ordovini, A. F.; Masuelli, Ricardo Williams; Overy, D. P.; et al.; First report of Penicillium allii as a field pathogen of garlic (Allium sativum); Wiley Blackwell Publishing, Inc; Plant Pathology; 55; 4; 8-2006; 583-583
0032-0862
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147639
identifier_str_mv Valdez, Jorge Gustavo; Makuch, M. A.; Ordovini, A. F.; Masuelli, Ricardo Williams; Overy, D. P.; et al.; First report of Penicillium allii as a field pathogen of garlic (Allium sativum); Wiley Blackwell Publishing, Inc; Plant Pathology; 55; 4; 8-2006; 583-583
0032-0862
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2006.01411.x/full
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-3059.2006.01411.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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