Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cells

Autores
Santiago Valtierra, Florencia Ximena; Mateos, Melina Valeria; Aveldaño, Marta Isabel; Oresti, Gerardo Martin
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rat spermatogenic cells contain sphingomyelins (SM) and ceramides (Cer) with very long chain (VLC) PUFA, in nonhydroxylated (n-V) and 2-hydroxylated (h-V) forms. How these atypical species distribute among membrane fractions during differentiation was investigated here using a detergent-free procedure to isolate a small light, raft-like, low density (L) fraction and a large heavy (H) fraction, mostly derived from the plasma membrane, of spermatocytes, round, and late spermatids. The L fraction contained cholesterol, glycerophospholipids and SM with the same saturated fatty acids in all three stages. In the H fraction, as PUFA increased in the glycerophospholipid and VLCPUFA in SM from spermatocytes to spermatids, the concentration of cholesterol also augmented. The H fraction had mostly n-V SM in spermatocytes but accumulated h-V SM and h-V Cer in spermatids. A fraction containing intracellular membranes had less SM and more Cer than H, but in both fractions SM and Cer species with h-V increased over species with n-V with differentiation. This accretion of h-V was consistent with the differentiation-dependent expression of fatty acid 2-hydroxylase (Fa2h), as it increased significantly from spermatocytes to spermatids. The non-raft region of the plasma membrane is thus the main target of the dynamic lipid synthesis and remodeling that is involved in germ cell differentiation.
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Materia
2-HYDROXY FATTY ACIDS
CERAMIDES
FATTY ACID HYDROXYLASE
MEMBRANE DOMAINS
SPERMATOGENESIS
SPHINGOMYELINS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41016
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41016
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cellsSantiago Valtierra, Florencia XimenaMateos, Melina ValeriaAveldaño, Marta IsabelOresti, Gerardo Martin2-HYDROXY FATTY ACIDSCERAMIDESFATTY ACID HYDROXYLASEMEMBRANE DOMAINSSPERMATOGENESISSPHINGOMYELINShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rat spermatogenic cells contain sphingomyelins (SM) and ceramides (Cer) with very long chain (VLC) PUFA, in nonhydroxylated (n-V) and 2-hydroxylated (h-V) forms. How these atypical species distribute among membrane fractions during differentiation was investigated here using a detergent-free procedure to isolate a small light, raft-like, low density (L) fraction and a large heavy (H) fraction, mostly derived from the plasma membrane, of spermatocytes, round, and late spermatids. The L fraction contained cholesterol, glycerophospholipids and SM with the same saturated fatty acids in all three stages. In the H fraction, as PUFA increased in the glycerophospholipid and VLCPUFA in SM from spermatocytes to spermatids, the concentration of cholesterol also augmented. The H fraction had mostly n-V SM in spermatocytes but accumulated h-V SM and h-V Cer in spermatids. A fraction containing intracellular membranes had less SM and more Cer than H, but in both fractions SM and Cer species with h-V increased over species with n-V with differentiation. This accretion of h-V was consistent with the differentiation-dependent expression of fatty acid 2-hydroxylase (Fa2h), as it increased significantly from spermatocytes to spermatids. The non-raft region of the plasma membrane is thus the main target of the dynamic lipid synthesis and remodeling that is involved in germ cell differentiation.Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaAmerican Society for Biochemistry and Molecular Biology2017-01-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41016Santiago Valtierra, Florencia Ximena; Mateos, Melina Valeria; Aveldaño, Marta Isabel; Oresti, Gerardo Martin; Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cells; American Society for Biochemistry and Molecular Biology; Jlr Papers In Press; 58; 12-1-2017; 529-5420022-22751539-7262CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jlr.org/content/58/3/529info:eu-repo/semantics/altIdentifier/doi/10.1194/jlr.M072595info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:21:45Zoai:ri.conicet.gov.ar:11336/41016instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:21:45.468CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cells
title Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cells
spellingShingle Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cells
Santiago Valtierra, Florencia Ximena
2-HYDROXY FATTY ACIDS
CERAMIDES
FATTY ACID HYDROXYLASE
MEMBRANE DOMAINS
SPERMATOGENESIS
SPHINGOMYELINS
title_short Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cells
title_full Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cells
title_fullStr Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cells
title_full_unstemmed Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cells
title_sort Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cells
dc.creator.none.fl_str_mv Santiago Valtierra, Florencia Ximena
Mateos, Melina Valeria
Aveldaño, Marta Isabel
Oresti, Gerardo Martin
author Santiago Valtierra, Florencia Ximena
author_facet Santiago Valtierra, Florencia Ximena
Mateos, Melina Valeria
Aveldaño, Marta Isabel
Oresti, Gerardo Martin
author_role author
author2 Mateos, Melina Valeria
Aveldaño, Marta Isabel
Oresti, Gerardo Martin
author2_role author
author
author
dc.subject.none.fl_str_mv 2-HYDROXY FATTY ACIDS
CERAMIDES
FATTY ACID HYDROXYLASE
MEMBRANE DOMAINS
SPERMATOGENESIS
SPHINGOMYELINS
topic 2-HYDROXY FATTY ACIDS
CERAMIDES
FATTY ACID HYDROXYLASE
MEMBRANE DOMAINS
SPERMATOGENESIS
SPHINGOMYELINS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rat spermatogenic cells contain sphingomyelins (SM) and ceramides (Cer) with very long chain (VLC) PUFA, in nonhydroxylated (n-V) and 2-hydroxylated (h-V) forms. How these atypical species distribute among membrane fractions during differentiation was investigated here using a detergent-free procedure to isolate a small light, raft-like, low density (L) fraction and a large heavy (H) fraction, mostly derived from the plasma membrane, of spermatocytes, round, and late spermatids. The L fraction contained cholesterol, glycerophospholipids and SM with the same saturated fatty acids in all three stages. In the H fraction, as PUFA increased in the glycerophospholipid and VLCPUFA in SM from spermatocytes to spermatids, the concentration of cholesterol also augmented. The H fraction had mostly n-V SM in spermatocytes but accumulated h-V SM and h-V Cer in spermatids. A fraction containing intracellular membranes had less SM and more Cer than H, but in both fractions SM and Cer species with h-V increased over species with n-V with differentiation. This accretion of h-V was consistent with the differentiation-dependent expression of fatty acid 2-hydroxylase (Fa2h), as it increased significantly from spermatocytes to spermatids. The non-raft region of the plasma membrane is thus the main target of the dynamic lipid synthesis and remodeling that is involved in germ cell differentiation.
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
description Rat spermatogenic cells contain sphingomyelins (SM) and ceramides (Cer) with very long chain (VLC) PUFA, in nonhydroxylated (n-V) and 2-hydroxylated (h-V) forms. How these atypical species distribute among membrane fractions during differentiation was investigated here using a detergent-free procedure to isolate a small light, raft-like, low density (L) fraction and a large heavy (H) fraction, mostly derived from the plasma membrane, of spermatocytes, round, and late spermatids. The L fraction contained cholesterol, glycerophospholipids and SM with the same saturated fatty acids in all three stages. In the H fraction, as PUFA increased in the glycerophospholipid and VLCPUFA in SM from spermatocytes to spermatids, the concentration of cholesterol also augmented. The H fraction had mostly n-V SM in spermatocytes but accumulated h-V SM and h-V Cer in spermatids. A fraction containing intracellular membranes had less SM and more Cer than H, but in both fractions SM and Cer species with h-V increased over species with n-V with differentiation. This accretion of h-V was consistent with the differentiation-dependent expression of fatty acid 2-hydroxylase (Fa2h), as it increased significantly from spermatocytes to spermatids. The non-raft region of the plasma membrane is thus the main target of the dynamic lipid synthesis and remodeling that is involved in germ cell differentiation.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41016
Santiago Valtierra, Florencia Ximena; Mateos, Melina Valeria; Aveldaño, Marta Isabel; Oresti, Gerardo Martin; Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cells; American Society for Biochemistry and Molecular Biology; Jlr Papers In Press; 58; 12-1-2017; 529-542
0022-2275
1539-7262
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41016
identifier_str_mv Santiago Valtierra, Florencia Ximena; Mateos, Melina Valeria; Aveldaño, Marta Isabel; Oresti, Gerardo Martin; Sphingomyelins and ceramides with very-long-chain PUFA are excluded from lowdensity, raft-like domains in differentiating spermatogenic cells; American Society for Biochemistry and Molecular Biology; Jlr Papers In Press; 58; 12-1-2017; 529-542
0022-2275
1539-7262
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jlr.org/content/58/3/529
info:eu-repo/semantics/altIdentifier/doi/10.1194/jlr.M072595
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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