Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis

Autores
Roychoudhry, Suruchi; Greenberg, Jean T.; Cecchini, Nicolas Miguel
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Understanding the generation, movement, uptake, and perception of mobile defense signals is key for unraveling the systemic resistance programs in flowering plants against pathogens. Here, we present a protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid (AZA) in Arabidopsis thaliana. We describe steps to assess 14C-AZA uptake into leaf discs and its movement from local to systemic tissues. We also detail the assay for uptake and movement of 2H-AZA from roots to the shoot.
Fil: Roychoudhry, Suruchi. University Of Leeds.; Reino Unido
Fil: Greenberg, Jean T.. University of Chicago. Department of Molecular Genetics and Cell Biology; Estados Unidos
Fil: Cecchini, Nicolas Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Materia
Arabidopsis
systemic defense
immunity
azelaic acid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/256586
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in ArabidopsisRoychoudhry, SuruchiGreenberg, Jean T.Cecchini, Nicolas MiguelArabidopsissystemic defenseimmunityazelaic acidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Understanding the generation, movement, uptake, and perception of mobile defense signals is key for unraveling the systemic resistance programs in flowering plants against pathogens. Here, we present a protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid (AZA) in Arabidopsis thaliana. We describe steps to assess 14C-AZA uptake into leaf discs and its movement from local to systemic tissues. We also detail the assay for uptake and movement of 2H-AZA from roots to the shoot.Fil: Roychoudhry, Suruchi. University Of Leeds.; Reino UnidoFil: Greenberg, Jean T.. University of Chicago. Department of Molecular Genetics and Cell Biology; Estados UnidosFil: Cecchini, Nicolas Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaElsevier2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/256586Roychoudhry, Suruchi; Greenberg, Jean T.; Cecchini, Nicolas Miguel; Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis; Elsevier; STAR Protocols; 5; 2; 6-2024; 1-132666-1667CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2666166724001096info:eu-repo/semantics/altIdentifier/doi/10.1016/j.xpro.2024.102944info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:43Zoai:ri.conicet.gov.ar:11336/256586instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:43.641CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis
title Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis
spellingShingle Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis
Roychoudhry, Suruchi
Arabidopsis
systemic defense
immunity
azelaic acid
title_short Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis
title_full Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis
title_fullStr Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis
title_full_unstemmed Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis
title_sort Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis
dc.creator.none.fl_str_mv Roychoudhry, Suruchi
Greenberg, Jean T.
Cecchini, Nicolas Miguel
author Roychoudhry, Suruchi
author_facet Roychoudhry, Suruchi
Greenberg, Jean T.
Cecchini, Nicolas Miguel
author_role author
author2 Greenberg, Jean T.
Cecchini, Nicolas Miguel
author2_role author
author
dc.subject.none.fl_str_mv Arabidopsis
systemic defense
immunity
azelaic acid
topic Arabidopsis
systemic defense
immunity
azelaic acid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Understanding the generation, movement, uptake, and perception of mobile defense signals is key for unraveling the systemic resistance programs in flowering plants against pathogens. Here, we present a protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid (AZA) in Arabidopsis thaliana. We describe steps to assess 14C-AZA uptake into leaf discs and its movement from local to systemic tissues. We also detail the assay for uptake and movement of 2H-AZA from roots to the shoot.
Fil: Roychoudhry, Suruchi. University Of Leeds.; Reino Unido
Fil: Greenberg, Jean T.. University of Chicago. Department of Molecular Genetics and Cell Biology; Estados Unidos
Fil: Cecchini, Nicolas Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
description Understanding the generation, movement, uptake, and perception of mobile defense signals is key for unraveling the systemic resistance programs in flowering plants against pathogens. Here, we present a protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid (AZA) in Arabidopsis thaliana. We describe steps to assess 14C-AZA uptake into leaf discs and its movement from local to systemic tissues. We also detail the assay for uptake and movement of 2H-AZA from roots to the shoot.
publishDate 2024
dc.date.none.fl_str_mv 2024-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/256586
Roychoudhry, Suruchi; Greenberg, Jean T.; Cecchini, Nicolas Miguel; Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis; Elsevier; STAR Protocols; 5; 2; 6-2024; 1-13
2666-1667
CONICET Digital
CONICET
url http://hdl.handle.net/11336/256586
identifier_str_mv Roychoudhry, Suruchi; Greenberg, Jean T.; Cecchini, Nicolas Miguel; Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis; Elsevier; STAR Protocols; 5; 2; 6-2024; 1-13
2666-1667
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2666166724001096
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.xpro.2024.102944
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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