HASTY-dependent miRNA cell-to-cell movement is required for systemic pathogen resistance in Arabidopsis

Autores
Musso, Manuel; Alanie, Nahir; Quevedo, Luciano; Trenchi, Alejandra; Cecchini, Nicolás Miguel; Lascano, Hernan Ramiro; Cambiagno, Damian Alejandro
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant defenses against pathogens are tightly regulated through complex gene expression control mecha- nisms. Precise activation and repression of defense-related genes are crucial for balancing the trade-off between growth and immunity. MicroRNAs (miRNAs) play a well-established role in the local regulation of plant–microbe interactions. While some miRNAs are also essential for systemic defense responses, their mechanisms of action, biogenesis, and long-distance mobility remain largely unexplored. Here, we show that HASTY (HST), a key factor in miRNA biogenesis and intercellular movement, is required for systemic defense activation. Impaired miRNA mobility in hst mutants correlates with the absence of systemic re- sponses. In infected tissues, HST may enhance the co-transcriptional processing of specific primary miR- NAs, thereby promoting cell-to-cell movement of their mature forms and contributing to systemic defense activation. Furthermore, two miRNAs that exhibit increased mobility during systemic defense induction are required for a proper systemic response. Notably, restoring miRNA mobility in the hst mutant without altering miRNA abundance, or complementing it with a version of HST expressed exclusively in companion cells, is sufficient to restore systemic defense induction, highlighting the critical role of miRNA cell-to-cell movement. Together, these findings elucidate the role of HST in plant immunity, linking miRNA biogenesis and mobility to the fine-tuned regulation of systemic defenses.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Musso, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Musso, Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Musso, Manuel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina
Fil: Alanie, Nahir. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina
Fil: Quevedo, Luciano. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Trenchi, Alejandra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina
Fil: Cecchini, Nicolás Miguel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina
Fil: Cecchini, Nicolás Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC); Argentina
Fil: Lascano, Hernan Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil:Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Cambiagno, Damian Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Cambiagno, Damian Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Cambiagno, Damian Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina
Fuente
Plant Communications 7 : 101777. (April 2026)
Materia
Arabidopsis
Immunity
Acquired Immunity
Plant Defence Reactions
Cell Communication
Inmunidad Adquirida
Reacción de Defensa de Plantas
Comunicación Celular
Inmunidad
HASTY
miRNA Mobility
Systemic Immunity
Cell-to-cell Signaling
miRNA Biogenesis
Plant-pathogen Interactions
microRNAs
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25706
Acceder
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oai_identifier_str oai:localhost:20.500.12123/25706
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling HASTY-dependent miRNA cell-to-cell movement is required for systemic pathogen resistance in ArabidopsisMusso, ManuelAlanie, NahirQuevedo, LucianoTrenchi, AlejandraCecchini, Nicolás MiguelLascano, Hernan RamiroCambiagno, Damian AlejandroArabidopsisImmunityAcquired ImmunityPlant Defence ReactionsCell CommunicationInmunidad AdquiridaReacción de Defensa de PlantasComunicación CelularInmunidadHASTYmiRNA MobilitySystemic ImmunityCell-to-cell SignalingmiRNA BiogenesisPlant-pathogen InteractionsmicroRNAsPlant defenses against pathogens are tightly regulated through complex gene expression control mecha- nisms. Precise activation and repression of defense-related genes are crucial for balancing the trade-off between growth and immunity. MicroRNAs (miRNAs) play a well-established role in the local regulation of plant–microbe interactions. While some miRNAs are also essential for systemic defense responses, their mechanisms of action, biogenesis, and long-distance mobility remain largely unexplored. Here, we show that HASTY (HST), a key factor in miRNA biogenesis and intercellular movement, is required for systemic defense activation. Impaired miRNA mobility in hst mutants correlates with the absence of systemic re- sponses. In infected tissues, HST may enhance the co-transcriptional processing of specific primary miR- NAs, thereby promoting cell-to-cell movement of their mature forms and contributing to systemic defense activation. Furthermore, two miRNAs that exhibit increased mobility during systemic defense induction are required for a proper systemic response. Notably, restoring miRNA mobility in the hst mutant without altering miRNA abundance, or complementing it with a version of HST expressed exclusively in companion cells, is sufficient to restore systemic defense induction, highlighting the critical role of miRNA cell-to-cell movement. Together, these findings elucidate the role of HST in plant immunity, linking miRNA biogenesis and mobility to the fine-tuned regulation of systemic defenses.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Musso, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Musso, Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Musso, Manuel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; ArgentinaFil: Alanie, Nahir. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; ArgentinaFil: Quevedo, Luciano. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Trenchi, Alejandra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal (IMBIV); ArgentinaFil: Cecchini, Nicolás Miguel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; ArgentinaFil: Cecchini, Nicolás Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC); ArgentinaFil: Lascano, Hernan Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil:Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Cambiagno, Damian Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Cambiagno, Damian Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Cambiagno, Damian Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; ArgentinaCell Press2026-04-08T09:49:58Z2026-04-08T09:49:58Z2026-02-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25706https://www.sciencedirect.com/science/article/pii/S25903462260008542590-3462https://doi.org/10.1016/j.xplc.2026.101777Plant Communications 7 : 101777. (April 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I083, Determinación de los mecanismos moleculares que rigen las interacciones entre plantas y patógenos y desarrollo de herramientas para contribuir al control de enfermedadesinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-04-16T09:53:30Zoai:localhost:20.500.12123/25706instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-04-16 09:53:31.208INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv HASTY-dependent miRNA cell-to-cell movement is required for systemic pathogen resistance in Arabidopsis
title HASTY-dependent miRNA cell-to-cell movement is required for systemic pathogen resistance in Arabidopsis
spellingShingle HASTY-dependent miRNA cell-to-cell movement is required for systemic pathogen resistance in Arabidopsis
Musso, Manuel
Arabidopsis
Immunity
Acquired Immunity
Plant Defence Reactions
Cell Communication
Inmunidad Adquirida
Reacción de Defensa de Plantas
Comunicación Celular
Inmunidad
HASTY
miRNA Mobility
Systemic Immunity
Cell-to-cell Signaling
miRNA Biogenesis
Plant-pathogen Interactions
microRNAs
title_short HASTY-dependent miRNA cell-to-cell movement is required for systemic pathogen resistance in Arabidopsis
title_full HASTY-dependent miRNA cell-to-cell movement is required for systemic pathogen resistance in Arabidopsis
title_fullStr HASTY-dependent miRNA cell-to-cell movement is required for systemic pathogen resistance in Arabidopsis
title_full_unstemmed HASTY-dependent miRNA cell-to-cell movement is required for systemic pathogen resistance in Arabidopsis
title_sort HASTY-dependent miRNA cell-to-cell movement is required for systemic pathogen resistance in Arabidopsis
dc.creator.none.fl_str_mv Musso, Manuel
Alanie, Nahir
Quevedo, Luciano
Trenchi, Alejandra
Cecchini, Nicolás Miguel
Lascano, Hernan Ramiro
Cambiagno, Damian Alejandro
author Musso, Manuel
author_facet Musso, Manuel
Alanie, Nahir
Quevedo, Luciano
Trenchi, Alejandra
Cecchini, Nicolás Miguel
Lascano, Hernan Ramiro
Cambiagno, Damian Alejandro
author_role author
author2 Alanie, Nahir
Quevedo, Luciano
Trenchi, Alejandra
Cecchini, Nicolás Miguel
Lascano, Hernan Ramiro
Cambiagno, Damian Alejandro
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis
Immunity
Acquired Immunity
Plant Defence Reactions
Cell Communication
Inmunidad Adquirida
Reacción de Defensa de Plantas
Comunicación Celular
Inmunidad
HASTY
miRNA Mobility
Systemic Immunity
Cell-to-cell Signaling
miRNA Biogenesis
Plant-pathogen Interactions
microRNAs
topic Arabidopsis
Immunity
Acquired Immunity
Plant Defence Reactions
Cell Communication
Inmunidad Adquirida
Reacción de Defensa de Plantas
Comunicación Celular
Inmunidad
HASTY
miRNA Mobility
Systemic Immunity
Cell-to-cell Signaling
miRNA Biogenesis
Plant-pathogen Interactions
microRNAs
dc.description.none.fl_txt_mv Plant defenses against pathogens are tightly regulated through complex gene expression control mecha- nisms. Precise activation and repression of defense-related genes are crucial for balancing the trade-off between growth and immunity. MicroRNAs (miRNAs) play a well-established role in the local regulation of plant–microbe interactions. While some miRNAs are also essential for systemic defense responses, their mechanisms of action, biogenesis, and long-distance mobility remain largely unexplored. Here, we show that HASTY (HST), a key factor in miRNA biogenesis and intercellular movement, is required for systemic defense activation. Impaired miRNA mobility in hst mutants correlates with the absence of systemic re- sponses. In infected tissues, HST may enhance the co-transcriptional processing of specific primary miR- NAs, thereby promoting cell-to-cell movement of their mature forms and contributing to systemic defense activation. Furthermore, two miRNAs that exhibit increased mobility during systemic defense induction are required for a proper systemic response. Notably, restoring miRNA mobility in the hst mutant without altering miRNA abundance, or complementing it with a version of HST expressed exclusively in companion cells, is sufficient to restore systemic defense induction, highlighting the critical role of miRNA cell-to-cell movement. Together, these findings elucidate the role of HST in plant immunity, linking miRNA biogenesis and mobility to the fine-tuned regulation of systemic defenses.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Musso, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Musso, Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Musso, Manuel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina
Fil: Alanie, Nahir. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina
Fil: Quevedo, Luciano. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Trenchi, Alejandra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal (IMBIV); Argentina
Fil: Cecchini, Nicolás Miguel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina
Fil: Cecchini, Nicolás Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC); Argentina
Fil: Lascano, Hernan Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil:Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Cambiagno, Damian Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Cambiagno, Damian Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Cambiagno, Damian Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina
description Plant defenses against pathogens are tightly regulated through complex gene expression control mecha- nisms. Precise activation and repression of defense-related genes are crucial for balancing the trade-off between growth and immunity. MicroRNAs (miRNAs) play a well-established role in the local regulation of plant–microbe interactions. While some miRNAs are also essential for systemic defense responses, their mechanisms of action, biogenesis, and long-distance mobility remain largely unexplored. Here, we show that HASTY (HST), a key factor in miRNA biogenesis and intercellular movement, is required for systemic defense activation. Impaired miRNA mobility in hst mutants correlates with the absence of systemic re- sponses. In infected tissues, HST may enhance the co-transcriptional processing of specific primary miR- NAs, thereby promoting cell-to-cell movement of their mature forms and contributing to systemic defense activation. Furthermore, two miRNAs that exhibit increased mobility during systemic defense induction are required for a proper systemic response. Notably, restoring miRNA mobility in the hst mutant without altering miRNA abundance, or complementing it with a version of HST expressed exclusively in companion cells, is sufficient to restore systemic defense induction, highlighting the critical role of miRNA cell-to-cell movement. Together, these findings elucidate the role of HST in plant immunity, linking miRNA biogenesis and mobility to the fine-tuned regulation of systemic defenses.
publishDate 2026
dc.date.none.fl_str_mv 2026-04-08T09:49:58Z
2026-04-08T09:49:58Z
2026-02-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25706
https://www.sciencedirect.com/science/article/pii/S2590346226000854
2590-3462
https://doi.org/10.1016/j.xplc.2026.101777
url http://hdl.handle.net/20.500.12123/25706
https://www.sciencedirect.com/science/article/pii/S2590346226000854
https://doi.org/10.1016/j.xplc.2026.101777
identifier_str_mv 2590-3462
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I083, Determinación de los mecanismos moleculares que rigen las interacciones entre plantas y patógenos y desarrollo de herramientas para contribuir al control de enfermedades
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv Plant Communications 7 : 101777. (April 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.692636

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