Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation

Autores
Ibañez, Maria Silvina; Camadro, Elsa Lucila; Sala, Carlos A.; Masuelli, Ricardo Williams
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Daucus pusillus Michx. and Daucus montevidensis Link ex Spreng. are wild carrots from the Americas with unresolved taxonomic status. An investigation was carried out with accessions of D. pusillus and (or) D. montevidensis from Argentina for (i) morphological and molecular (amplified fragments length polymorphism (AFLP) and inter simple sequence repeat (ISSR)) characterization, (ii) analysis of congruence of morphological and molecular variation, and (iii) comparison of diversity for the internal transcribed spacer (ITS) region with that reported for a North American accession of D. pusillus. Twelve accessions of D. pusillus and (or) D. montevidensis ? representing their geographical distribution in Argentina ? and one accession each of wild Daucus carota L. and Daucus montanus Humb. & Bonpl. ex Schult. ? as outgroups ? were included. In the multivariate analysis of morphological diversity, two accessions were clearly differentiated; this result is not sustained by multivariate analysis of molecular diversity. Based on multivariate and molecular variance (AMOVA) analyses, D. pusillus and (or) D. montevidensis accessions were separated at the molecular level into two groups, associated with geographical origin. Because this result is not supported by morphology, the segregation into two taxa seems unjustified. In all accessions, ITS and 5.8S rDNA regions had identical sequences, which differ in one nucleotide from the corresponding sequence of the North American accession. According to the combined results, D. pusillus would be a single taxon distributed from North to South America, and D. montevidensis a nomenclatural synonym. Autogamy of D. pusillus and its highly structured genetic diversity (Fst = 0.86) allows the application of a geographically targeted approach for germplasm exploration, conservation and eventual use in pre-breeding.
Fil: Ibañez, Maria Silvina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sala, Carlos A.. Nidera S.A. Departamento de Biotecnología; Argentina
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Materia
Wild Carrots
Genetics Resources
Molecular Diversity
Morphological Diversity
Its Sequence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23017
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/23017
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network_name_str CONICET Digital (CONICET)
spelling Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservationIbañez, Maria SilvinaCamadro, Elsa LucilaSala, Carlos A.Masuelli, Ricardo WilliamsWild CarrotsGenetics ResourcesMolecular DiversityMorphological DiversityIts Sequencehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Daucus pusillus Michx. and Daucus montevidensis Link ex Spreng. are wild carrots from the Americas with unresolved taxonomic status. An investigation was carried out with accessions of D. pusillus and (or) D. montevidensis from Argentina for (i) morphological and molecular (amplified fragments length polymorphism (AFLP) and inter simple sequence repeat (ISSR)) characterization, (ii) analysis of congruence of morphological and molecular variation, and (iii) comparison of diversity for the internal transcribed spacer (ITS) region with that reported for a North American accession of D. pusillus. Twelve accessions of D. pusillus and (or) D. montevidensis ? representing their geographical distribution in Argentina ? and one accession each of wild Daucus carota L. and Daucus montanus Humb. & Bonpl. ex Schult. ? as outgroups ? were included. In the multivariate analysis of morphological diversity, two accessions were clearly differentiated; this result is not sustained by multivariate analysis of molecular diversity. Based on multivariate and molecular variance (AMOVA) analyses, D. pusillus and (or) D. montevidensis accessions were separated at the molecular level into two groups, associated with geographical origin. Because this result is not supported by morphology, the segregation into two taxa seems unjustified. In all accessions, ITS and 5.8S rDNA regions had identical sequences, which differ in one nucleotide from the corresponding sequence of the North American accession. According to the combined results, D. pusillus would be a single taxon distributed from North to South America, and D. montevidensis a nomenclatural synonym. Autogamy of D. pusillus and its highly structured genetic diversity (Fst = 0.86) allows the application of a geographically targeted approach for germplasm exploration, conservation and eventual use in pre-breeding.Fil: Ibañez, Maria Silvina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sala, Carlos A.. Nidera S.A. Departamento de Biotecnología; ArgentinaFil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; ArgentinaNatl Research Council Canada-n R C Research Press2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23017Ibañez, Maria Silvina; Camadro, Elsa Lucila; Sala, Carlos A.; Masuelli, Ricardo Williams; Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation; Natl Research Council Canada-n R C Research Press; Botany; 92; 5; 1-2014; 348-3591916-2790CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1139/cjb-2013-0241info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/abs/10.1139/cjb-2013-0241#.WaB7Bvk1-JAinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:28Zoai:ri.conicet.gov.ar:11336/23017instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:28.288CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation
title Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation
spellingShingle Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation
Ibañez, Maria Silvina
Wild Carrots
Genetics Resources
Molecular Diversity
Morphological Diversity
Its Sequence
title_short Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation
title_full Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation
title_fullStr Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation
title_full_unstemmed Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation
title_sort Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation
dc.creator.none.fl_str_mv Ibañez, Maria Silvina
Camadro, Elsa Lucila
Sala, Carlos A.
Masuelli, Ricardo Williams
author Ibañez, Maria Silvina
author_facet Ibañez, Maria Silvina
Camadro, Elsa Lucila
Sala, Carlos A.
Masuelli, Ricardo Williams
author_role author
author2 Camadro, Elsa Lucila
Sala, Carlos A.
Masuelli, Ricardo Williams
author2_role author
author
author
dc.subject.none.fl_str_mv Wild Carrots
Genetics Resources
Molecular Diversity
Morphological Diversity
Its Sequence
topic Wild Carrots
Genetics Resources
Molecular Diversity
Morphological Diversity
Its Sequence
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Daucus pusillus Michx. and Daucus montevidensis Link ex Spreng. are wild carrots from the Americas with unresolved taxonomic status. An investigation was carried out with accessions of D. pusillus and (or) D. montevidensis from Argentina for (i) morphological and molecular (amplified fragments length polymorphism (AFLP) and inter simple sequence repeat (ISSR)) characterization, (ii) analysis of congruence of morphological and molecular variation, and (iii) comparison of diversity for the internal transcribed spacer (ITS) region with that reported for a North American accession of D. pusillus. Twelve accessions of D. pusillus and (or) D. montevidensis ? representing their geographical distribution in Argentina ? and one accession each of wild Daucus carota L. and Daucus montanus Humb. & Bonpl. ex Schult. ? as outgroups ? were included. In the multivariate analysis of morphological diversity, two accessions were clearly differentiated; this result is not sustained by multivariate analysis of molecular diversity. Based on multivariate and molecular variance (AMOVA) analyses, D. pusillus and (or) D. montevidensis accessions were separated at the molecular level into two groups, associated with geographical origin. Because this result is not supported by morphology, the segregation into two taxa seems unjustified. In all accessions, ITS and 5.8S rDNA regions had identical sequences, which differ in one nucleotide from the corresponding sequence of the North American accession. According to the combined results, D. pusillus would be a single taxon distributed from North to South America, and D. montevidensis a nomenclatural synonym. Autogamy of D. pusillus and its highly structured genetic diversity (Fst = 0.86) allows the application of a geographically targeted approach for germplasm exploration, conservation and eventual use in pre-breeding.
Fil: Ibañez, Maria Silvina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sala, Carlos A.. Nidera S.A. Departamento de Biotecnología; Argentina
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
description Daucus pusillus Michx. and Daucus montevidensis Link ex Spreng. are wild carrots from the Americas with unresolved taxonomic status. An investigation was carried out with accessions of D. pusillus and (or) D. montevidensis from Argentina for (i) morphological and molecular (amplified fragments length polymorphism (AFLP) and inter simple sequence repeat (ISSR)) characterization, (ii) analysis of congruence of morphological and molecular variation, and (iii) comparison of diversity for the internal transcribed spacer (ITS) region with that reported for a North American accession of D. pusillus. Twelve accessions of D. pusillus and (or) D. montevidensis ? representing their geographical distribution in Argentina ? and one accession each of wild Daucus carota L. and Daucus montanus Humb. & Bonpl. ex Schult. ? as outgroups ? were included. In the multivariate analysis of morphological diversity, two accessions were clearly differentiated; this result is not sustained by multivariate analysis of molecular diversity. Based on multivariate and molecular variance (AMOVA) analyses, D. pusillus and (or) D. montevidensis accessions were separated at the molecular level into two groups, associated with geographical origin. Because this result is not supported by morphology, the segregation into two taxa seems unjustified. In all accessions, ITS and 5.8S rDNA regions had identical sequences, which differ in one nucleotide from the corresponding sequence of the North American accession. According to the combined results, D. pusillus would be a single taxon distributed from North to South America, and D. montevidensis a nomenclatural synonym. Autogamy of D. pusillus and its highly structured genetic diversity (Fst = 0.86) allows the application of a geographically targeted approach for germplasm exploration, conservation and eventual use in pre-breeding.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23017
Ibañez, Maria Silvina; Camadro, Elsa Lucila; Sala, Carlos A.; Masuelli, Ricardo Williams; Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation; Natl Research Council Canada-n R C Research Press; Botany; 92; 5; 1-2014; 348-359
1916-2790
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23017
identifier_str_mv Ibañez, Maria Silvina; Camadro, Elsa Lucila; Sala, Carlos A.; Masuelli, Ricardo Williams; Morphological and molecular diversity of the wild carrot Daucus pusillus: implications for classification and ex situ conservation; Natl Research Council Canada-n R C Research Press; Botany; 92; 5; 1-2014; 348-359
1916-2790
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1139/cjb-2013-0241
info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/abs/10.1139/cjb-2013-0241#.WaB7Bvk1-JA
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Natl Research Council Canada-n R C Research Press
publisher.none.fl_str_mv Natl Research Council Canada-n R C Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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