Genetic structure and domestication of carrot (Daucus carota subsp. sativus) (Apiaceae)

• Autores: Iorizzo, Massimo; Senalik, Douglas A.; Ellison, Shelby L.; Grzebelus, Dariusz; Cavagnaro, Pablo; Allender, Charlotte; Brunet, Johanne; Spooner, David M.; Deynze, Allen Van; Simon, Philipp W.
• Año de publicación: 2013
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: American Journal of Botany 100 (5) : 930-938 (May 2013)
• Materia: Zanahoria; Daucus Carota; Domesticación de Plantas; Genética; Variación Genética; Carrots; Plant Domestication; Genetics; Genetic Structures; Genetic Variation; Estructura Genética
• Institución: Instituto Nacional de Tecnología Agropecuaria

• Premise of the study: Analyses of genetic structure and phylogenetic relationships illuminate the origin and domestication of modern crops. Despite being an important worldwide vegetable, the genetic structure and domestication of carrot (Daucus carota) is poorly understood. We provide the first such study using a large data set of molecular markers and accessions that are widely dispersed around the world. • Methods: Sequencing data from the carrot transcriptome were used to develop 4000 single nucleotide polymorphisms (SNPs). Eighty‐four genotypes, including a geographically well‐distributed subset of wild and cultivated carrots, were genotyped using the KASPar assay. • Key results: Analysis of allelic diversity of SNP data revealed no reduction of genetic diversity in cultivated vs. wild accessions. Structure and phylogenetic analysis indicated a clear separation between wild and cultivated accessions as well as between eastern and western cultivated carrot. Among the wild carrots, those from Central Asia were genetically most similar to cultivated accessions. Furthermore, we found that wild carrots from North America were most closely related to European wild accessions. • Conclusions: Comparing the genetic diversity of wild and cultivated accessions suggested the absence of a genetic bottleneck during carrot domestication. In conjunction with historical documents, our results suggest an origin of domesticated carrot in Central Asia. Wild carrots from North America were likely introduced as weeds with European colonization. These results provide answers to long‐debated questions of carrot evolution and domestication and inform germplasm curators and breeders on genetic substructure of carrot genetic resources.
EEA La Consulta
Fil: Iorizzo, Massimo. University of Wisconsin. Department of Horticulture; Estados Unidos
Fil: Senalik, Douglas A. University of Wisconsin. Department of Horticulture; Estados Unidos. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos
Fil: Ellison, Shelby L. University of Wisconsin. Department of Horticulture; Estados Unidos
Fil: Grzebelus, Dariusz. University of Agriculture in Krakow. Department of Genetics, Plant Breeding and Seed Science; Polonia
Fil: Cavagnaro, Pablo Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Allender, Charlotte. University of Warwick. Warwick Crop Centre; Gran Bretaña
Fil: Brunet, Johanne. United States Department Of Agriculture. Agriculture Research Service; Vegetable Crops Research Unit; Estados Unidos. University of Wisconsin. Department of Entomology; Estados Unidos
Fil: Spooner, David M. University of Wisconsin. Department of Horticulture; Estados Unidos. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos
Fil: Deynze, Allen Van. University of California. Seed Biotechnology Center; Estados Unidos
Fil: Simon, Philipp W. University of Wisconsin. Department of Horticulture; Estados Unidos. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos